Inhibition of Protease Activity in Muscle Extracts and Surimi from Pacific Whiting, Merluccius productus, and Arrowtooth Flounder, Atheresthes stomias

Muscle extracts of Pacific whiting, Merluccius productus, and arrowtooth flounder, Atheresthes stomias, were assayed for proteolytic activity using azocasein as a substrate. Pacific whiting extracts showed maximum activity at pH 5.0-5.2 and a temperature of 50°C, while arrowtooth flounder extracts had maximum activity at pH 5.5 and 55°C. Three sources of inhibitors (potatoes, egg white, beef plasma protein) were evaluated in vitro for inhibition of protease activity. All three were found to be effective inhibitors in crude muscle extracts. Further studies utilizing these inhibitors in surimi showed that potato was equivalent to both egg white and beef plasma protein in preserving the gel forming characteristics ofheated kamaboko in both species.

The Fisheries of Hawaii and U.S.-associated Pacific Islands

In the late 1980's and early 1990's, significant changes occurred in the fisheries of Hawaii. Expansion and diversification of pelagic fisheries and growth (including industrialization) of fisheries that, in at least some cases, had been largely recreational or artisanall ed to fear of overfishing and problems in allocation among fishery sectors. Combined with establishment of limited entry programs in Hawaii fisheries (bottomfish, longline, and lobster), this led to anticipation that similar growth might occur in Guam, the Northern Marianas, and American Samoa.

Fisheries and Marine Resources of Hawaii and the U.S.-associated Pacific Islands: An Introduction

The fisheries of Hawaii and other U.S.-associated islands in the Pacific Ocean are characterized by high diversity, both in the species exploited and the human cultures that exploit them. The commercial sector has undergone rapid growth in recent years, but recreational and subsistence sectors remain important. Information on these fisheries is generally not available in published form. This paper presents an overview and introduction to a volume of papers describing fisheries in the region, with the goal of making the information available to scientists and the general public. A great deal remains to be learned about the dynamics of these fisheries as well as the associated issues in biological research, fisheries management, and environmental protection.

Precious Coral Fisheries of Hawaii and the U.S. Pacific Islands

The precious coral fishery in Hawaii and the Western Pacific consists of one industry but two distinct and separate fisheries. The first is the harvest of black coral by scuba divers from depths of 30-100 m. The second is a fishery for pink and gold coral at depths between 400 and 1500 m and employs either a human-operated submersible that permits selective harvest or tangle net dredges which are nonselective. The modern history of these fisheries date from 1958 until the present. In this paper the ecology, life history. and management of the dominant species that make up these fisheries are reviewed. Research needs of the fisheries and the economic and future prospects of the precious coral industry are also described. At the present, the precious coral jewelry industry in Hawaii (all species) is valued at about $25 million at the retail level.

The Western Pacific Fishery Information Network: A Fisheries Information System

The Western Pacific Fishery Information Network (WPACFlN) is an intergovernmental agency cooperative program sponsored by the National Marine Fisheries Service (NMFS) to help participating island fisheries agencies carry out data collection, analysis, reporting programs, and data management activities to better support fisheries management under the Magnuson Fishery Conservation and Management Act; and to help meet local fisheries information and management needs. The WPACFlN is the central source of information for Federal fisheries management of most fisheries in American Samoa, Guam, and the Northern Mariana Islands, and it plays an important role in acquiring fisheries data in Hawaii. This paper describes the development and status of this fishery information system.

Club Penguin: a produção de subjetividade capitalística na narrativa interativa nos videogames

Na pesquisa Club Penguin: a produção de subjetividade capitalística na narrativa interativa nos videogames vamos realizar a cartografia (Deleuze & Guattari) do gameplay do Club Penguin, em sua plataforma principal, online. É uma pesquisa em andamento, já que o jogo não tem um objetivo único a ser alcançado e sua narrativa não é conclusiva, pois depende integralmente do agenciamento entre formas de pensar, agir e sentir do usuário com a programação do jogo, produzindo um rizoma que nunca se fecha. Para poder verificar seus efeitos na produção da subjetividade capitalística das primeiras pistas que seguimos foi a presença das tecnologias de informação e comunicação, que, ao mesmo tempo em que permitem a autonomia do usuário, exercem uma vigilância e um controle consentidos, produzindo dados que servem de base para a manutenção e preservação da sociedade de controle (Gilles Deleuze). Nossa segunda pista está ligada às práticas e laços que se formam no decorrer do jogo, que refletem os efeitos das relações presentes no capitalismo contemporâneo, que tem como base o consumo e a produção de signos e afetos, tal como descritas por Hardt & Negri. O percurso da terceira pista está ligado às linhas de enunciação e visibilidade cujos efeitos se refletem nos processos de subjetivação das crianças que participam do jogo, visto que o público alvo deste jogo está na faixa dos 6 aos 14 anos de idade.

Obra-jogo: processos, visualidades e tessituras

Esta dissertação trata das visualidades buscadas no campo da arte contemporânea utilizando como linguagem o videogame e sua estética, que se formam como transfigurações das ideias de um mundo estruturado em códigos, uma informação entre os signos que convivem no cotidiano do homem comum. Neste caráter, o trabalho artístico aliado ao videogame propõe estados de percepção estética e interação com o espectador como forma estrutural da obra, como a obra em si. Unir a arte com o videogame para alcançar a mistura entre artista, obra e espectador

Differences in Dolphin Mortality Rates in Night and Day Sets for the U.S. Eastern Tropical Pacific Tuna Purse Seine Fishery

Because dolphins sometimes travel with yellowfin tuna, Thunnus albacares, in the eastern tropical Pacific (ETP), purse seiners use the dolphins to locate and capture tuna schools. During the process of setting the purse seine nets, dolphins often become entangled and drown before they can be released. Data for the U.S. purse seine fleet in the ETP during 1979-88 show that dolphin mortality rates in sets made during the night are higher than mortality rates in sets made during the day. Even with efforts to reduce nightset mortality rates through the use of high intensity floodlights, night set mortality rates remain higher. The data are also used to simulate a regulation on the fishery aimed at eliminating night sets and show that dolphin mortality rates would decrease.

Pacific Salmon, Oncorhynchus spp., and the Definition of "Species" Under the Endangered Species Act

For purposes ofthe Endangered Species Act (ESA), a "species" is defined to include "any distinct population segment of any species of vertebrate fish or wildlife which interbreeds when mature. "Federal agencies charged with carrying out the provisions of the ESA have struggled for over a decade to develop a consistent approach for interpreting the term "distinct population segment." This paper outlines such an approach and explains in some detail how it can be applied to ESA evaluations of anadromous Pacific salmonids. The following definition is proposed: A population (or group of populations) will be considered "distinct" (and hence a "species ")for purposes of the ESA if it represents an evolutionarily significant unit (ESU) of the biological species. A population must satisfy two criteria to be considered an ESU: 1) It must be substantially reproductively isolated from other conspecific population units, and 2) It must represent an important component in the evolutionary legacy of the species. Isolation does not have to be absolute, but it must be strong enough to permit evolutionarily important differences to accrue in different population units. The second criterion would be met if the population contributes substantially to the ecological/genetic diversity of the species as a whole. Insights into the extent of reproductive isolation can be provided by movements of tagged fish, natural recolonization rates observed in other populations, measurements of genetic differences between populations, and evaluations of the efficacy of natural barriers. Each of these methods has its limitations. Identification of physical barriers to genetic exchange can help define the geographic extent of distinct populations, but reliance on physical features alone can be misleading in the absence of supporting biological information. Physical tags provide information about the movements of individual fish but not the genetic consequences of migration. Furthermore, measurements ofc urrent straying or recolonization rates provide no direct information about the magnitude or consistency of such rates in the past. In this respect, data from protein electrophoresis or DNA analyses can be very useful because they reflect levels of gene flow that have occurred over evolutionary time scales. The best strategy is to use all available lines of evidence for or against reproductive isolation, recognizing the limitations of each and taking advantage of the often complementary nature of the different types of information. If available evidence indicates significant reproductive isolation, the next step is to determine whether the population in question is of substantial ecological/genetic importance to the species as a whole. In other words, if the population became extinct, would this event represent a significant loss to the ecological/genetic diversity of thes pecies? In making this determination, the following questions are relevant: 1) Is the population genetically distinct from other conspecific populations? 2) Does the population occupy unusual or distinctive habitat? 3) Does the population show evidence of unusual or distinctive adaptation to its environment? Several types of information are useful in addressing these questions. Again, the strengths and limitations of each should be kept in mind in making the evaluation. Phenotypic/life-history traits such as size, fecundity, and age and time of spawning may reflect local adaptations of evolutionary importance, but interpretation of these traits is complicated by their sensitivity to environmental conditions. Data from protein electrophoresis or DNA analyses provide valuable insight into theprocessofgenetic differentiation among populations but little direct information regarding the extent of adaptive genetic differences. Habitat differences suggest the possibility for local adaptations but do not prove that such adaptations exist. The framework suggested here provides a focal point for accomplishing the majorgoal of the Act-to conserve the genetic diversity of species and the ecosystems they inhabit. At the same time, it allows discretion in the listing of populations by requiring that they represent units of real evolutionary significance to the species. Further, this framework provides a means of addressing several issues of particular concern for Pacific salmon, including anadromous/nonanadromous population segments, differences in run-timing, groups of populations, introduced populations, and the role of hatchery fish.

Biology and Potential Use of Pacific Grenadier, Coryphaenoides acrolepis, off California

Grenadiers (family Macrouridae) are the most abundant fish on most continental slope areas worldwide. Off California the Pacific grenadier, Coryphaenoides acrolepis, occurs in relatively large numbers and may have marketing potential. This repon provides information on the biology of the species and catch results from a number of scientific cruises. Catch data on several other species found together with Pacific grenadier, panicularly sablefish, Anoplopoma fimbria, are also given. The fish were caught with a bottom trawl (15 trawls), and with free-vehicle longline gear (117 sets). The latter was a hook and line system in which the gear was dropped to the seafloor untethered to the fishing vessel, and floated to the surface, with the catch, when detachable weights were automatically released. Sablefish dominated longline catches in depths of 200-600fm (334-1,098m), while Pacific grenadier was most abundant between 600 and 1,OOOfm (1,098-1,830m). Best trawl catches of Pacific grenadier were made at depths between 615 and 675fm (1,125 and 1,235 m) and at 760fm (1,391 m). Ripe females were absent from our samples, but spent females were found during the entire year with highest numbers in the spring and early summer. Only one larva was found despite extensive sampling with plankton nets. Pacific grenadier was found to have good edible qualities by a taste-test panel, although the protein content (15 percent) and flesh yield (24 percent) were significantly lower than those of other fishes. A second species, the giant grenadier, Albatrossia pectoralis, was found to have exceptionally poor eating qualities and even lower protein content.

A Review of the South Pacific Tuna Baitfisheries: Small Pelagic Fisheries Associated with Coral-Reefs

A review is given of current information concerning small pelagic fishes exploited for tuna bait in the South Pacific. These fishes are usually caught over or near coral reefs using light attraction and lift nets. The most common and widespread species are anchovies (Engraulidae), sprats (Clupeidae), silversides (Atherinidae), and herrings (Clupeidae). Recorded yields ranged from 0.5 to 2.6t/km2, and methods are described to estimate potential yields empirically in the absence of catch data. Environmental effects on small pelagic fish production are discussed, and evidence is presented to suggest that rainfall markedly affects stolephorid anchovy production. Some species of small pelagic fish, such as Selar spp., Decapterus spp., and Herklotsichthys sp., have been fished traditionally by artisanal fishermen, but anchovy and sprat stocks were probably unexploited prior to pole-and-line tuna fishing in the South Pacific.

U.S. Fisheries Management and Foreign Trade Linkages: Policy Implications for Groundfish Fisheries in the North Pacific EEZ

The groundfish resources of the U.S. Exclusive Economic Zone (EEZ) off Alaska, dominated by Alaska or walleye pollock, Theragra chalcogramma, Pacific cod, Gadus macrocephalus, and flatfishes, Pleuronectidae, can sustain annual commercial harvests well in excess of 2 million metric tons (t). As recently as 1979, foreign fisheries took 99 percent of the annual harvest supported by these resources. This has changed dramatically during the 1980's. The foreign fisheries have received rapidly decreasing allocations, first as joint venture fisheries expanded and, more recently, as the domestic fisheries have grown. Joint venture fisheries are fisheries in which domestic fishing vessels deliver their catch directly to foreign processing vessels in the EEZ. By 1986, the joint venture and domestic fisheries accounted for 66 percent and 8 percent, respectively, of the annual harvest. The preliminary corresponding figures for 1987 are 78 and 18 percent.