Effects of weapons procurement stretch-outs on costs and schedules

Item 1005-C

How changes in fiscal policy affect the budget : the feedback issue.

Mode of access: Internet.

Space science research in the United States.

"OTA-TM-STI-19"--P. [4] of cover.

Reference listing of 1992 federal tax forms and publications.

Includes indexes.

The grazing bulletin.

Lithographed, March, 1936-

How do we affect taxpayer behavior? : the case for positive incentives, assistance, or enforcement /

"Catalog #64956T"--Cover.

Characteristics of food stamp work registrants.

"May 1987."

Works of Robert Burns: with an account of his life, and criticism on his writings. to which are prefixed, some observtions on the character and condition of the Scottish peasantry.

Mode of access: Internet.

Transportation of hazardous materials.

Mode of access: Internet.

Vista de la bahía de Alicante [Material gráfico] = Vue de la rade d'Alicante = View of the harbour of Alicant

Resumen: Descrición: vista de la bahía de la ciudad de Alicante, en segundo término varios barcos

Functional and biophysical analysis of the C-terminus of the CGRP-receptor; a family B GPCR

G-protein coupled receptors (GPCRs) typically have a functionally important C-terminus which, in the largest subfamily (family A), includes a membrane-parallel eighth helix. Mutations of this region are associated with several diseases. There are few C-terminal studies on the family B GPCRs and no data supporting the existence of a similar eighth helix in this second major subfamily, which has little or no sequence homology to family A GPCRs. Here we show that the C-terminus of a family B GPCR (CLR) has a disparate region from N400 to C436 required for CGRP-mediated internalization, and a proximal region of twelve residues (from G388 to W399), in a similar position to the family A eighth helix, required for receptor localization at the cell surface. A combination of circular and linear dichroism, fluorescence and modified waterLOGSY NMR spectroscopy (SALMON) demonstrated that a peptide mimetic of this domain readily forms a membrane-parallel helix anchored to the liposome by an interfacial tryptophan residue. The study reveals two key functions held within the C-terminus of a family B GPCR and presents support for an eighth helical region with striking topological similarity to the nonhomologous family A receptor. This helix structure appears to be found in most other family B GPCRs.

A qualitative evaluation of the Scottish Staff and Associate Specialist Development Programme

Acknowledgements Our thanks to all those who took part in the interviews, and to Gillian Campbell for her help in organising these interviews. Our thanks to NHS Education for Scotland for commissioning us to carry out this project. Funding Our thanks to NHS Education for Scotland (NES) for funding this research. JC and PJ received no financial support for the research, authorship, and/or publication of this article.