The influence of nitrogen and phosphorus on the growth of a diatom Skeletonema costatum (Greville) Cleve

Nitrogen and phosphorus requirements of a chain-forming diatom, Skeletonema costatum (Greville) Cleve, collected from Yatsushiro Sea, Japan, were investigated in a laboratory culture experiment. Sodium nitrate and sodium glycerophosphate were used as nitrogen and phosphorus sources, respectively. Cultures were grown in modified Provasoli's ASP2NTA medium (Provasoli et al. 1957) at 25±1°C, light intensity 60 µE mˉ² secˉ¹ and photoperiod 12:12-h, L:D cycle. Optimum growth was observed at nitrate concentrations of 3-10 mglˉ¹ and phosphate concentrations of 1.5-15 mglˉ¹. Adequate growth was also found at the nitrate concentration of up to as high as 300 mglˉ¹. Significantly poorer growth was found at lower nitrate (<3.0 mglˉ¹) and higher phosphate (>15 mglˉ¹) concentrations. From the present study, it is concluded that S. costatum can grow well at wide ranges of nitrate concentrations but is sensitive to higher phosphate concentrations.

Physical soil characteristics of waterlogged and saline lands of five districts of Haryana, India

Physical characteristics of waterlogged and saline soils of five districts, namely Rohtak, Faridabad, Gurgaon, Sonipat and Jhajjar, of Haryana were studied to evaluate the aquaculture potential. The soil samples from 76 sites were procured and analysed for pH, electrical conductivity, soil particles and water retention capacity, since the knowledge of these parameters is essential for the preliminary evaluation of a site for aqua-farming. Six soil types were identified and clay-loam was observed to be the maximum at 45 (59.21%) sites, followed by silty-clay loam at 14 (18.42%), sandy-clay loam at six (7.89%), silty-loam at four (5.26%), sandy-loam at four (5.26%) and loam at three (3.94%) sites. The pH of soil indicated moderate to high alkaline conditions ranging between 8.01and 9.00 at 53 (69.73%) sites, 9.01 and 10.00 at 17 (22.36%) sites, and low between 7.01 and 8.00 at six (7.89%) sites. The electrical conductivity was found to range between <1 and 10 mmho cmˉ¹ with the value of <1.0 mmho cmˉ¹ at 36 (47.36%) sites. The water retention capacity was observed mostly to be moderate. The paper describes the interrelationship between these parameters with reference to the suitability for aqua-farming.