Molecular differentiation between Salmonella enterica subsp enterica serovar Pullorum and Salmonella enterica subsp enterica serovar Gallinarum

A S. Pullorum (SP) é muito semelhante à S. Gallinarum (SG), agentes da Pulorose e Tifo aviário, respectivamente, sendo que as duas enfermidades são responsáveis por perdas econômicas no setor avícola. SP e SG são de difícil diferenciação em procedimento laboratorial rotineiro, mas uma prova bioquímica muito utilizada na distinção das duas refere-se à capacidade de assimilar o aminoácido ornitina: SP descarboxila este aminoácido enquanto SG não. No entanto, o isolamento de cepas com comportamento bioquímico atípico, tem dificultado tal diferenciação. Um dos genes relacionados à assimilação do aminoácido ornitina, denomina-se gene speC, o qual está presente nos dois sorovares. Analisando 21 amostras de SP e 15 de SG com a utilização da PCR não foi possível realizar a diferenciação dos dois sorovares pois os fragmentos gerados eram idênticos. Posteriormente, com o uso da técnica de tratamento enzimático com a enzima de restrição Eco RI, foi possível observar que o padrão de bandas gerado em cada sorovar era diferente, mesmo quando amostras que apresentavam comportamento bioquímico atípico eram analisadas. Tal fato permitiu a padronização da técnica para ser utilizada na diferenciação entre os sorovares Pullorum e Gallinarum de maneira rápida e segura.

A Tessitura de sentidos: representação social de licenciados do curso deletras da UFRN sobre o ensinar

En esta investigación, se busca comprender los procesos de formación de pregrado del Curso de Cartas da UFRN, de estas representaciones sociales acerca de los estudiantes de enseñanza. Por lo tanto, el objetivo fue conocer y analizar las representaciones sociales de curso de pregrado UFRN Cartas sobre la enseñanza. Por lo tanto, se aplica la Técnica de Libre de Palabras Asociación (TALP) con 20 participantes, después de haber sido probados individualmente, utilizando como estímulo palabra "enseñar" y limitar el número de invocaciones a 03 palabras por participante. Esta técnica fue elegida porque es una técnica proyectiva que permite la reducción y racionalización permite acceder al campo semántico de las representaciones sociales, en nuestro caso, en la enseñanza del de pregrado do Curso de Cartas da UFRN. Por lo tanto, llegamos a total de 60 llamadas, análisis de frecuencia después de 41 y 24 después de un análisis del significado que se utilizaron en otro procedimiento aplicado en esta investigación, las calificaciones de Procedimiento de Múltiples Calificaciones (PCM). Estas palabras, sobre la base de las justificaciones de las evocaciones y significados que atribuyen participantes en la investigación, se dividieron en 4 campos semánticos: Campo 1: Aspectos técnicos de la enseñanza, Campo 2: aspectos volitivos, Campo 3: aspectos relacionales de la enseñanza y de Campo 4: Elementos del campo. El PCM se aplicó de forma individual, con 50 estudiantes. Para el análisis de los datos generados, utilizamos Análisis Multidimensional (MDS) para la Escala Multidimensional de Evaluación Análisis gratuito (MSA: Multidimensional Scalogram Analysis) y el análisis de clasificación selectiva de los espacios menores (SSA: Smallest Space Analysis). El mapa generado por el análisis de MSA fue dividido en tres regiones: Aspectos de los aspectos de enseñanza, capacitación y asistencia técnica de los promotores de la profesionalización, la primera región que tiene una sub-regionalización llamada dimensión negativa. El mapa de la SSA análisis se dividió en dos partes y un subfaceta, llamado el aspecto faceta técnica de la enseñanza y la formación, la segunda faceta llamado Vínculo entre el campo y la enseñanza específica y su nombre subfaceta aspecto relacional. Del análisis del contenido de las justificaciones de la PCM, llegó a 5 categorías, a saber: Hacer la enseñanza, la faceta negativa, Escuela, Formación y faceta afectiva del profesor. El TALP aporta pruebas de que algunos de estos estudiantes ve la oportunidad de adquirir unos conocimientos en esta área, la formación en un nivel superior, como quieran, simplemente tienen un título universitario para realizar licitación abierta. El PCM nos ha hecho comprender que el contenido representacional en cuestión se configura desde el universo consensual conectado a la profesión y la formación. Por lo tanto, podemos decir que los titulares de licencias con respecto al tema estudiado anclar la representación social de "enseñar" a la imagen de ser un maestro y objetivar la imagen de la profesión

A Escola sob o olhar da família: relações que se compreendem e se praticam no cotidiano

This study approaches the relations of family and school, from the perspective of the family. It aims to analyze the meanings attributed by family to the school as a space for plural relationships, and understand the relations that these mothers constitute culturally with schools and their everyday pedagogical practices. When we seek to understand this problematic, it was established as guiding principles of the search in/from/with everyday life. Accordingly, we focused on, as main interlocutors, mothers and authors such as Certeau (1994), Morin (2000) and Freire (1978), among others, on the understanding that cultural diversity is an essential element of the complex relationship between family and public school, because they come from different cultural contexts. In the course of the research it was made the use of the procedure of dialogic conversation as a production process and information analysis. The current analysis highlights that families observe the difference between cultural and report that they are from different socio-political context set by the school, they came from rural areas and their lives were marked by the struggle for survival, and the work activity has been present since their childhood. For the families that participated in this research the school is/was not part of the cultural repertoire significance in their lives and it feels that they attribute to them, are in negotiations with their symbolic universe. The mothers hold a speech of a schoolar binding and pertencing, but the school has the time as possible in their daily lives. Thus, the dynamics of family and school relationships, is configured as complex, and the ambiguity in maternal speech marks a thinking/doing about the school in which they demonstrate ways of making the common man, involving the art of duplicity of the saying and doing, gimmnicks and antidiscipline

Comparação de Meios de Enriquecimento e de Plaqueamento Utilizados na Pesquisa de Salmonella em Carcaças de Frango e Fezes de Aves

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Narrativas de formação de professoras dos anos iniciais do ensino fundamental: Concepções e construções de necessidades formativas na Geografia Escolar

This article is about a study about the training needs for teachers of elementary school in the field of Geography. It is our objective to grasp their conceptions of training needs and reflect about their formative needs to teach geography. We consider the training as reflective process that involves the movement of changes and improvement beyond of formal learning, considering its numerous dimensions. We reflected about formative needs in light of the readings of Rodrigues Esteves (1993), Silva (2000), Roberts (2006), Vieira (2010). The discussions about conceptions were based on Ferreira (2007). The empirical reference constitutes to a private school in the city of Ceará-Mirim/RN, SECAT Centro de Ensino. The social subjects of our research are five teachers who work in the initial years of elementary school. We resorted a survey (auto) biographical, based on the studies of Passeggi (2011), Delory (2008), Bertaux (2010) e Josso (2010), since it is our intention to turn to the historicity of the subject and the learning, recognizing the links between him and the world and the experiences based on for learning and adult training. As technical and methodological procedure we will use the Training Narratives, whose application allows the understanding of memories and stories of schooling teachers, since are reported events occurred during the development of the individual through seminars Biographical. We observed on the narratives constructed by the teachers the absence assignments of meanings to a reworking of the theoretical formative needs and questions of its organizing principles. However, we notice the teachers were able to develop senses and means to conceive the phenomenon in study, in a descriptive way, through articulated enunciations, including aspects and opportunities linked to their teaching practices and future formative prospects. Regarding School Geography, we based our studies in the reflections of Smith Junior (1994, 2000), Tonini (2003), Vesentini (2004) and Vlach (1991), among others. We verified that the needs evidenced by the teachers to teach geography were constructed from the contexts of their teaching practices present in their school and professional trajectories. Therefore, we noticed the need for formal pedagogical qualification so that we can conceive the phenomenon in study beyond its descriptive character, understanding that it is necessary to point out reflections and questions about the dynamics of production of global capital, which conveys its interests in the contexts that often emerge formative needs of the educational system.