Historia Bibliothecae Regiae Academiae Aboensis 16

Invokaatio: D.A.G.

De bibliothecariis Academiae Aboënsis 16

Arkit: 1 arkintunnukseton lehti, U4.

Apologeticus Dei 16

Invokaatio: In nomine Jesu!

Lytrodoxia Jesu 16

Variantti B.

Enhanced anti-tumor effect of a gene gun-delivered DNA vaccine encoding the human papillomavirus type 16 oncoproteins genetically fused to the herpes simplex virus glycoprotein D

Anti-cancer DNA vaccines have attracted growing interest as a simple and non-invasive method for both the treatment and prevention of tumors induced by human papillomaviruses. Nonetheless, the low immunogenicity of parenterally administered vaccines, particularly regarding the activation of cytotoxic CD8+ T cell responses, suggests that further improvements in both vaccine composition and administration routes are still required. In the present study, we report the immune responses and anti-tumor effects of a DNA vaccine (pgD-E7E6E5) expressing three proteins (E7, E6, and E5) of the human papillomavirus type 16 genetically fused to the glycoprotein D of the human herpes simplex virus type 1, which was administered to mice by the intradermal (id) route using a gene gun. A single id dose of pgD-E7E6E5 (2 µg/dose) induced a strong activation of E7-specific interferon-γ (INF-γ)-producing CD8+ T cells and full prophylactic anti-tumor effects in the vaccinated mice. Three vaccine doses inhibited tumor growth in 70% of the mice with established tumors. In addition, a single vaccine dose consisting of the co-administration of pgD-E7E6E5 and the vector encoding interleukin-12 or granulocyte-macrophage colony-stimulating factor further enhanced the therapeutic anti-tumor effects and conferred protection to 60 and 50% of the vaccinated mice, respectively. In conclusion, id administration of pgD-E7E6E5 significantly enhanced the immunogenicity and anti-tumor effects of the DNA vaccine, representing a promising administration route for future clinical trials.

Disquisitiones practicae 16-17

Nimekettä edeltää hepreankielinen invokaatio.

Zohar hattebhah Gen. VI:16. seu fenestra in arca diluviana

Invokaatio: I.N.J.

Chronicon episcoporum Finlandensium 16

Arkit: 1 arkintunnukseton lehti, 2H-2I4.

Placental hydroxymethylation vsmethylation at the imprinting control region 2 on chromosome 11p15.5

In addition to methylated cytosines (5-mCs), hydroxymethylcytosines (5-hmCs) are present in CpG dinucleotide-enriched regions and some transcription regulator binding sites. Unlike methylation, hydroxymethylation does not result in silencing of gene expression, and the most commonly used methods to study methylation, such as techniques based on restriction enzymatic digestion and/or bisulfite modification, are unable to distinguish between them. Genomic imprinting is a process of gene regulation where only one member of an allelic pair is expressed depending on the parental origin. Chromosome 11p15.5 has an imprinting control region (ICR2) that includes a differentially methylated region (KvDMR1) that guarantees parent-specific gene expression. The objective of the present study was to determine the presence of 5-hmC at the KvDMR1 in human placentas. We analyzed 16 third-trimester normal human placentas (chorionic villi). We compared two different methods based on real-time PCR after enzymatic digestion. The first method distinguished methylation from hydroxymethylation, while the other method did not. Unlike other methylation studies, subtle variations of methylation in ICRs could represent a drastic deregulation of the expression of imprinted genes, leading to important phenotypic consequences, and the presence of hydroxymethylation could interfere with the results of many studies. We observed agreement between the results of both methods, indicating the absence of hydroxymethylation at the KvDMR1 in third-trimester placentas. To the best of our knowledge, this is the first study describing the investigation of hydroxymethylation in human placenta using a genomic imprinting model.

Expositio transformationis lacrymarum Heliadum in electrum, exercitatione academica adumbrata, atque ex consensu ampliss. facult. philos. In regia ad Auram Academia, praeside viro celeberrimo Andrea Prysz, poës. prof. reg. & ordin. Bonorum censurae subjecta â Johanne Franseen, Kexh. Carel. In auditorio majori die 16 Junij, anni 1708

Invokaatio: I.N.J.

Insecta Fennica enumerans 1:16

Painovuosi nimekkeestä.

Theses (1824-06-16)

Painovuosi nimekkeestä.

Sensus dicti, Luc. XVI: vers. 16. eruens

Invokaatio: Q.F.F.Q.S.

De justificatione Christi in spiritu, occasione dicti I. Tim. III: comm. 16

Invokaatio: D.D.

De virtute evangelii Christi, Rom. I: 16

Invokaatio: I.N.J.