Stimulation of polygalacturonase production in an immobilized system by Aspergillus sp.: effect of pectin and glucose

The synthesis of polygalacturonases (PG) is known to be influenced by Aspergillus growth conditions, namely, environmental factors and pectin content in the cultivation medium containing a mixed carbon source. Optimal conditions were attained at a temperature of 30 A degrees C and an initial pH of 4.5. PG activity (3.29 and 2.48 U/mL) was determined after a two-day culture of Aspergillus sp. HC1 and Aspergillus sp. CC1, respectively, in a basic medium containing 2% citrus pectin as the sole carbon source. The addition of glucose (2% w/v) to the basic medium led to a 2-fold increase in PG production. However, enzyme synthesis was repressed when a higher concentration of glucose was used in the medium containing the mixed carbon source. Spores from the two fungi were immobilized in a 3% Ca-alginate system and the mechanical strength of the gel beads allowed the use of this process system 6-fold longer (288 h) than the free culture. In the Aspergillus sp. CC1 immobilized system, PG production increased nearly 10-fold in the medium with 2% glucose added (5.95 U/mL) in comparison to the medium without sugar (0.55 U/mL). The results demonstrate that a different response in activity was produced by free and entrapped spore systems. PG production remained approximately constant throughout the six 48 h cycles in the medium containing citrus pectin (2% w/v) as the sole carbon source.

Relationships among Pratylenchus jaehni and P. coffeae populations from Brazil.

Wilcken, S.R.S., E.S. Mori, M. Bacci, L.C.C.B. Ferraz, C.M.G. Oliveira & M.M. Inomoto. 2008. Relationships among Pratylenchus jaehni and P. coffeae populations from Brazil. The relationships among Pratylenchus jaehni (C) and six amphimitic Pratylenchus populations from Brazil (three from banana, PcB1, PcB2, and PcB3; one from Aglaonema sp., M2; one from coffee, K5; and one from citrus, C) were studied through morphological and molecular analysis, and pathogenicity test. The analysis of morphometric characteristics of PcB1, M2, C2 and K5, compared with P. jaehni and P. coffeae (K6) data obtained from literature, delineated three groups: P. coffeae (K6), PcB1 and M2; P. jaehni (C1) and C2; and K5. RAPD analysis of PcB1, PcB2, PcB3, M2, C2, K5 and P.jaehni (C1) demonstrated that these populations form three distinct genetic groups: PcB1, PcB2, PcB3 and M2; P.jaehni and C2; and K5. In pathogenicity test, K5 reproduced well on coffee and caused extensive root necrosis, but C2 did not. Population C2 reproduced well on Rangpur lime, which was previously rated as poor host to K5. Therefore, the results demonstrated that C2 is P. jaehni, K5 is an undescribed species of Pratylenchus, and PcB1, PcB2 and PcB3 are P. coffeae. The taxonomic status of M2 remains inconclusive.

Doenças respiratórias em aves atendidas no laboratório de oriento- Patologia da FMVZ-UNESP/Botucatu-SP, Brasil, nos anos de 2005 A 2006

The respiratory tract of birds shows anatomical and physiological characteristics that facilitates the occurrence of disease, mainly those of microbiological origin. This article analyzes the frequency of respiratory diseases in domestic and wild birds treated during the years 2005-2006 at the Ornitopathology Laboratory of the Veterinary Hospital, Faculty of Veterinary Medicine and Zootecnics, São Paulo State University (FMVZUNESP). Diagnoses were obtained through clinical and radiographic exams as well as haemogram, microbiological culture, parasitological tests and necropsy, being septicemic processes, aspergillosis and mycoplasmosis the most diagnosed diseases. No zoonotic threats were identified, so the entities were described as of low-impact on public health. However, the above mentioned agents can provoke death of birds and difficult for treating and eradication as well.

Avaliação Comparativa da Agressividade de Meloidogyne javanica e M. incognita à Variedade SP 911049 de Cana-de-açúcar

Nematodes cause extensive losses to sugarcane in Brazil and also in other producing regions. Meloidogyne incognita, M. javanica and Pratylenchus zeae are the key species for this culture worldwide. In the present study, the aggressiveness of M. javanica and M. incognita to sugarcane variety SP 911049 was evaluated comparatively,. The following parameters were compared: reproduction factor (RF) of these nematodes, effect of nematodes in the natural incidence of pests, and the influence on the development and technological characteristics of sugarcane. Considering the data of RF, biometrics, natural infestation of pests, mortality of plants, and technological variables, it was concluded that M. javanica was more aggressive to sugarcane, although its rate of multiplication was much smaller than the one of M. incognita.

Baixa diversidade de zooplâncton na desembocadura de uma represa eutrófica (SP, Brasil): reflexo da poluição?

The Tiete River (in São Paulo state) receives most of the efuents of the metropolitan region of São Paulo; and the Barra Bonita reservoir, 200 km distant downstream, receives and recycle most of that pollution load. This work studied the ecological features of the zooplankton (Rotifera, Cladocera and Copepoda) sampled in a point of Tiete River's mouth. Monthly samples were collected, from April, 2001 to February, 2008. The hypothesis currently tested was that the composition and availability of zooplankton were similar to the others reservoir regions (middle and lacustrine sensu Thornton) and to other reservoirs of the Upper Parana River basin, supporting the idea that these organisms manage to stand the strong pollution. The reservoir shows a typical variation of accumulation, with wide variation of seasonal altimetric grading and large fow in the rainy pe- riod, infuenced by the rainfall regime, with efects upon zooplankton assemblage. As a whole, 24 species were registered: 12 Rotifera, 8 Cladocera and 4 Copepoda. Data showed that the hypothesis formulated must be rejected, due the variation trends of zooplankton environmental attributes not being regular, with relatively high abundance values for a lotic environment in specifc months, and low organism abundance or absence in other months. Those facts can be attributed to pollution efects conducting to low dissolved oxygen concentration (> 4mg.l-1) in some months, which causes the zooplankton community to be very much afected. [K]

Caracteristicas de padroes de drenagem de areas com entalhes erosivos no municipio de Botucatu - SP

Descriptive and quantitative characteristics of the drainage pattern of two places very susceptible to erosive process, located in the municipality of Botucatu-SP, were studied through photo-interpretative methods. The analysis and discussion of the obtained results led to the conclusion that the geologic control in the area B, masking the effect of the relief and terrain permeability, increases the drainage density, the length of channels and the angularity of the channels of 3rd and 4th order. -from English summary

Probabilidade de ocorrencia de periodos secos e chuvosos em Rio Claro - SP.

Using the daily rainfall data from 1961 through 1980 the frequency of dry and wet periods was determined. The results of the frequency distribution of dry and wet periods indicated that observed data fit very closely an equation of the type Y = aebn. -after English summary

Mapeamento das formacoes superficiais da Regiao de Sao Pedro- Piracicaba (SP, Brasil).

As criteria in photointerpretation slope features, tonality, texture, structure, vegetation density and gullies were utilized. The origin of the surficial formations is related to the weathering, erosion and deposition processes and little diagenesis.-after English summary

Analise espacial de populacao: o caso da depressao demografica na Regiao da Alta Paulistra (Marilia-SP).

The population redistribution processes considered at a micro level, such as that for the Upper-Paulista Railroad micro-region (Marilia) in Brazil, shows that for nearly 50 years there was accelerated demographic growth followed immediately afterwards by varied declines involving the rural and urban populations.-from English summary

Intrusao alcalina de Jaboticabal, SP.

Five magnetic anomalies were found and mapped in the Faculdade de Ciencias Agrarias e Veterinarias, UNESP, campus of Jaboticabal, SP. The study showed that those anomalies represent intrusive alkaline bodies linked to magmatic manifestation with regional expression. -English summary

Cobertura vegetal do Municipio de Taquaritinga, SP, Antes da expansao da cultura da cana-de-acucar.

Occurrence and distribution of 12 natural and anthropic communities were mapped.-from English summary

Spontaneous triploidy and NOR activity in Eigenmannia sp. (Pisces, Sternopygidae) from the Amazon basin

The occurrence of a natural triploid among diploid individuals of Eigenmannia sp from the Amazon basin is described. The diploid individuals had 2n=31 and 32 chromosomes, the difference in chromosome number being due to a Robertsonian rearrangement. The triploid individual presented 2n=46 and had two fused acrocentrics. NOR activity was present in the three homologues of triploid and a NOR polymorphism was detected in the triploid and diploids. © 1985 Dr W. Junk Publishers.

Numero de dias de ocorrencia de orvalho em Botucatu, SP.

This paper analyses the dew occurrence in Botucatu, Sao Paulo State, Brazil, in the period from 1971 to 1984. The phenomenon was observed on the grass plot of the Climatological Station in the 'Presidente Medici' Experimental Farm. The station is installed on soil 'Terra Roxa Estruturada', on a middle slope, declivity of 8% and east exposure. Days with dew were summed for the following periods: month, year, season, no rainy period and rainy period. For each period the following values were calculated: mean, mean standard errors and mean confidence interval. Also the absolute extreme values and the amplitude of variation were determined. The confidence interval of the mean were calculated by the 't' statistic, at the level of 5% of error probability. The means were compared by using the 't' statistic, at the level of 5% of error probability. The following table of values corresponds to the regime of dew occurrence in Botucatu, expressed in number of days with dew. The dew occurrence regime in Botucatu was analysed comparatively with the regime at Itatinga, in Sao Paulo State and with the regime of Rio Grande, in Rio Grande do Sul State. -English summary

Condicionamento geologico da agua subterranea em Sao Jose,do Rio Preto, SP.

A study of the geological characteristics of the Buaru Group basaltic substratum and stratigraphical unities was carried out, taking into account the groundwater exploration in S.Jose do Rio Preto area. The presence of the tectonic structures in this substratum exercised a profound influence on the sedimentation of the Bauru Group and on the variations in the hydrological properties of the sediments of the area.-English summary

Consequencias da modernizacao da agricultura: o uso indiscriminado de praguicidas no municipio de Rio Claro (SP)

The modernization process has been introduced in the agricultural sector without any technical control. This process has provoked loss of food quality, serious damage to the worker's health and environmental degradation. The lack of technical assistance is total and farmers are not interested in the hazards involved. -after English summary