Lectina da esponja marinha Tedania ignis: purificação, caracterização e interação com leishmanias

Lectin obtained from the marine sponge Tedania ignis was purified and characterized by extraction of soluble proteins (crude extract) in 50mM Borax, pH 7.5. The purification procedure was carried out by crude extract precipitation with ammonium sulfate 30% (FI). The precipitated was resuspended in the same buffer and fractionated with acetone 1.0 volume (F1.0). A lectin was purified from this specific fraction by using an affinity chromatography Sepharose 6B. This lectin preferentially agglutinated human erythrocytes from B type previously treated with papain enzyme. The hemagglutinating activity lectin was dependent of divalent Mn2+ cation and was inhibited by the carbohydrates galactose, xylose and fructose. SDS-PAGE analysis indicated a molecular mass of the lectin around 45 kDa. This protein showed stability until 40°C for 1 h. Further, it showed activity between pH 2.5 and 11.5, with an enhanced activity at pH 7.5. Leishmania chagasi promastigotes stained with Coomassie brilliant blue R-250 were agglutinated by F1,0 and in the presence of galactose this interaction was abolished. These results show that this lectin could be implicated in defense procedures and it will can be used as biological tools in studies with this protozoon

“Dasein” ansiedade perante a morte e imortalidade simbólica em idosos institucionalizados

Dissertação de Mestrado apresentada ao Instituto Superior de Psicologia Aplicada para obtenção de grau de Mestre na especialidade de Psicologia Clínica.

Interação de microorganismos na solubilização de fósforo e potássio de rochas para produção de biofertilizantes

he present model of agriculture is based on intensive use of industrial inputs, due to its rapid response, but it brings harmful consequences to the environment, and it is necessary the use of modern inputs. And an alternative is the use of rock biofertilizers in agriculture, a product easy to use, with higher residual effect and does not harm the environment. The objective of study was to evaluate the inoculation and co-inoculation of different microorganisms in the solubilization of rock phosphate and potash ground microbial evaluating the best performance in the production of biofertilizers comparing with rocks pure in soil chemical properties and, verify effect of inoculation of the bacterium Paenibacillus polymyxa in the absorption of minerals dissolved in the development of cowpea (Vigna unguiculata [L.] Walp.). The first bioassay was conducted in Laboratory (UFRN) for 72 days in Petri dishes, where the rock powder was increased by 10% and sulfur co-inoculated and inoculated with bacterial suspension of Paenibacillus polymyxa grown in medium tryptone soy broth, Ralstonia solanacearum in medium Kelman, Cromobacterium violaceum in medium Luria-Bertani and Acidithiobacillus thiooxidans in medium Tuovinen and Kelly,and fungi Trichoderma humatum and Penicillium fellutanum in malt extract. Every 12 days, samples were removed in order to build up the release curve of minerals. The second bioassay was conducted in a greenhouse of the Agricultural Research Corporation of Rio Grande do Norte in experimental delineation in randomized block designs, was used 10 kg of an Yellow Argissolo Dystrophic per pot with the addition of treatments super phosphate simple (SS), potassium chloride (KCl), pure rock, biofertilizers in doses 40, 70, 100 and 200% of the recommendation for SS and KCl, and a control, or not inoculated with bacteria P. polymyxa. Were used seeds of cowpea BRS Potiguar and co-inoculated with the bacterial suspension of Bradyrhizobium japonicum and P. polymyxa. The first crop was harvested 45 days after planting, were evaluated in the dry matter (ADM), macronutrients (N, P, K, Ca, Mg) and micronutrients (Zn, Fe, Mn) in ADM. And the second at 75 days assessing levels of macro end micronutrients in plants and soil, and the maximum adsorption capacity of P in soil. The results showed synergism in co-inoculations with P. polymyxa+R. solanacearum and, P. polymyxa+C. violaceum solubilizations providing higher P and K, respectively, and better solubilization time at 36 days. The pH was lower in biofertilizers higher doses, but there was better with their addition to P at the highest dose. Significant reduction of maximum adsorption capacity of phosphorus with increasing dose of biofertilizer. For K and Ca was better with SS+KCl, and Mg to pure rock. There was an effect of fertilization on the absorption, with better results for P, K and ADM with SS+KCL, and N, Ca and Mg for biofertilizers. Generally, the P. polymyxa not influence the absorption of the elements in the plant. In treatments with the uninoculated P. polymyxa chemical fertilizer had an average significantly higher for weight and number of grains. And in the presence of the bacteria, biofertilizers and chemical fertilizers had positive values in relation to rock and control. The data show that the rocks and biofertilizers could meet the need of nutrients the plants revealed as potential for sustainable agriculture

Trabalho e família: um estudo da interação de papéis com secretários executivos

The balance between the demands of two important spheres of human life, work and family, has become a challenge due to the pressures of the contemporary that is expanding around the difficulties of reconciling these two comínios. In this sense, this research aimed to understand the work-family interaction in the perception of executive secretaries. The analysis approach used was qualitative research, by worrying about a reality that can not be quantified due to the subjectivity of his goal. The data collection technique used was the semistructured interview to twenty executive secretaries, servants of a Federal Institution of Higher Education. For the understanding and interpretation of the data, we used the technique of content analysis. The results of both analyzes identified the existence of conflict as enrichment in this interaction. The time was identified as the largest generator of conflict work. The overload, relationship stress and conflict emerged as elements common to both domains. As main implications of labor disputes, were revealed: problamas health for secretaries and stress. As main implications of family conflicts emerged: motivation for work, lower performance and lack of concentration. The attempt at balance was identified as the strategy most used by secretaries to minimize work-family conflict. The work-family enrichment was seen as resources that contribute to improving the lives of the secretary in both domains. The opportunity to add knowledge was highlighted as enriching element of work and family values ​​as elements enriching family. The support and experience emerged as enrichment items common to both domains. Regarding the implications of enrichment resulting from the interaction of work and family, the more perceived by respondents were: increased knowledge and skills, material and psychological benefits, improved quality of life and personal and professional fulfillment. From the perception of executive secretaries, work and family spheres of human life are essential and complementary, and that help is contrary, however, this relationship is the primary management of conflicts, ie, how the individual sees and manages the negative side of the work-family

Ensaio sobre a cegueira : as regras do método jornalístico e a reprodução simbólica da realidade nos enquadramentos das notícias sobre a crise econômica em Portugal

Tese (doutorado)—Universidade de Brasília, Instituto de Ciências Sociais, Departamento de Sociologia, 2014.

Agrupamentos verticais: a interação dos professores do ensino básico

Dissertação de mest. em Observação e Análise da Relação Educativa, Faculdade de Ciências Humanas e Sociais, Univ. do Algarve, 2004

Subjetividade e interação nos fóruns online reflexões sobre a permanência em educação a distância

Este artigo foi desenvolvido com objetivo de produzir indicadores que possam melhorar a funcionalidade dos fóruns online e contribuir numa maior permanência dos estudantes da Educação a Distância. Foi realizada uma análise, orientada pela Epistemologia Qualitativa, dos processos subjetivos e interacionais produzidos nos fóruns de apresentação e fóruns temáticos de duas disciplinas de formação pedagógica – (1) Estratégias de Ensino e Aprendizagem e (2) A Psicologia e a Construção do Conhecimento – ofertadas nos cursos de Licenciatura em Teatro, Música e Artes Visuais, UAB/UnB. As informações produzidas apontam para a necessidade de reconhecimento e valorização do estudante como sujeito na aprendizagem, a consolidação da presença pedagógica do tutor, a valorização dos fóruns como espaços de aprendizagem e a produção de espaços sociais de pertencimento. ______________________________________________________________________________ ABSTRACT

Análise da interação entre moléculas desenhadas a partir do ácido anacárdico e a proteína PPAR usando ferramentas in silico

Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Biologia Molecular, 2016.

Avaliação do glifosato na interação entre Phakopsora pachyrhizi e Glycine max por meio do ácido Chiquímico. II: efeito curativo.

2009

Avaliação do glifosato na interação entre Phakopsora pachyrhizi e Glycine max por meio do ácido Chiquímico. I: efeito preventivo

2009

Gestão compartilhada de P&D em petróleo:a interação entre a Petrobrás e a Universidade Federal do Rio Grande do Norte

This work addresses the relationship between University-Firm aims to understand the model of shared management of R&D in petroleum of Petrobras with UFRN. This is a case study which sought to investigate whether the model of cooperation established by the two institutions brings innovation to generate technical-scientific knowledge and contribute to the coordination with other actors in the promotion of technological innovation. In addition to desk research the necessary data for analysis were obtained by sending questionnaires to the coordinators of projects in R&D at the company and university. Also, interviews were conducted with subjects who participated in the study since its inception to the present day. This case study were analysed through the Resource-Based View and Interorganizational Networks theory. The sample data also stands that: searches were aligned to the strategic planning and that 29% of R&D projects have been successful on the scope of the proposed objectives (of which 11% were incorporated into business processes); which was produced technical and scientific knowledge caracterized by hundreds of national and international publications; thesis, dissertations, eleven patents, and radical and incremental innovations; the partnership has also brought benefits to the academic processes induced by the improved infrastructure UFRN and changing the "attitude" of the university (currently with national prominence in research and staff training for the oil sector). As for the model, the technical point of view, although it has some problems, it follows that it is appropriate. From the viewpoint of the management model is criticized for containing an excess of bureaucracy. From the standpoint of strategic allocation of resources from the legal framework needs to be reassessed, because it is focused only on the college level and it is understood that should also reach the high school given the new reality of the oil sector in Brazil. For this it is desirable to add the local government to this partnership. The set of information leads to the conclusion that the model is identified and named as a innovation of organizational arrangement here known as Shared Management of R&D in petroleum of Petrobras with UFRN. It is said that the shared management model it is possible to exist, which is a simple and effective way to manage partnerships between firms and Science and Technology Institutions. It was created by contingencies arising from regulatory stand points and resource dependence. The partnership is the result of a process of Convergence, Construction and Evaluation supported by the tripod Simplicity, Systematization and Continuity, important factors for its consolidation. In practice an organizational arrangement was built to manage innovative university-industry partnership that is defined by a dyadic relationship on two levels (institutional and technical, therefore governance is hybrid), by measuring the quarterly meetings of systematic and standardized financial contribution proportional to the advancement of research. These details have led to the establishment of a point of interaction between the scientific and technological-business dimension, demystifying they are two worlds apart

Proposta de modelo de interação da Universidade Federal do Rio Grande do Norte e empresas do setor de petróleo

Operational procedures may negatively interfere in negotiation and execution regarding universities and business companies. In some cases it may even derail business interaction. Thus, aiming to overcome this and other barriers a university-industry interaction model was structured. The model enhances the appropriation of technological solutions on behalf of enterprises, as well as aim to improve the quality of teaching and research done at the university. In order to conduct a case study, sampling considering the Federal University of Rio Grande do Norte (UFRN) was made as well as the Oil and Gas sector. For data collection questionnaires, classroom observation, document analysis, semi-structured interviews were used. The study describes the companies as well as the internal organization of UFRN in their interaction context. The diagnosis related to past interactions as well as the expectations of the companies and the university s internal subjects regarding the university-industry relationship were also studied. Thus, specific questionnaires were applied for the three types of groups: researchers, managers and business companies. These subjects pointed out that the great deal of barriers they identified were related to issues regarding the university internal management. Given these barriers, the critical factors were then identified in order to overcome this reality. Among the nine critical factors only one belongs to the macro environment, while the remaining factors are related to organizational issues present in the university context. It was possible to formulate a university-business interaction model one the researched focused on the case study results and contribution from a theoretical framework that was enabled trough literature review. The model considers all business collaboration mechanisms; it focuses on a particular strategic productive sector and provides a co-evolution vision over time, according to the sector´s development strategy. The need for institutionalizing the relationship with the companies involved is pointed out. The proposed model considers all the critical factors identified by the research; it aims long-term relationship with the company and integrates teaching, research and extension actions. The model implementation was also considered. It was seen that it must be done in three phases. The phases will be defined by the level of maturity in the relationship between the university and the companies. Thus, a framework was developed in order to assess the interaction level regarding company institutionalization. Whilst structuring the model was a concern with replication came up. It was pointed out that this model should not only serve to this specific case study situation. So the final result is a model of university-industry relationship appropriate in the first instance, for UFRN, but has applicability, in general, to any Brazilian university

Interação Médico Veterinário – Enfermeiro Veterinário no exercício da profissão

A procura do Curso de Medicina Veterinária pelas classes jovens prende-se essencialmente com o gosto pela área da saúde e o interesse pelos animais. A inexistência de alternativa à Medicina Veterinária, único Curso no nosso país em 2002, para quem gostasse de tratar e cuidar de animais, num âmbito diferente do da produção, fez com que muitos jovens passassem pela frustração de se dedicarem a outras áreas. Com o objetivo de colmatar essa lacuna do panorama Veterinário nacional, proporcionando aos amantes dos animais uma opção alternativa a quem aspirava poder praticar cuidados de saúde veterinários e não tinha possibilidade de aceder à carreira de Medicina Veterinária, fazia sentido preencher este vazio com a criação de uma nova carreira intermédia, de índole técnico, profissionalizante e superior e, enquanto por todo o país se optava pela criação de mais cursos de Medicina Veterinária, os Politécnicos despertaram para este novo nicho de mercado promissor, inspirados pela Europa, que permitiria a formação de quadros bacharéis, integráveis em equipas Médico-veterinárias e às centenas de profissionais que já exerciam essas funções, por todo o país, sem formação adequada, a oportunidade de dignificação da sua profissão. O Curso de Enfermagem Veterinária foi aprovado em Portugal em 2002/03 para funcionar na Escola Superior Agrária de Elvas e reprovado para funcionar na Escola Superior Agrária de Viseu, no mesmo ano, sendo na altura Ministro da Ciência e Ensino Superior o Doutor Pedro Lynce Faria. Em 2003/04 funcionava o seu primeiro ano na Escola Superior Agrária de Elvas, quando finalmente a Escola Superior Agrária de Viseu também obteve a aprovação para a proposta enviada no ano anterior, colocando o curso em funcionamento um ano mais tarde (2004/05). Posteriormente, viria a entrar em funcionamento nas Escolas Superiores Agrárias de Bragança (Tecnologia Veterinária 2005/06; Enfermagem Veterinária 2010/11), Castelo Branco (2006/07) e Ponte de Lima (2006/07). Até então cabia exclusivamente ao Médico Veterinário uma grande diversidade de funções, que incluíam o atendimento ao público, a receção dos animais e seus proprietários, a procura e preenchimento de fichas clínicas, a contenção e pesagem, o cálculo e administração de dosagens terapêuticas, a monitorização anestésica, a preparação e disposição de material cirúrgico, a preparação dos animais para cirurgia e a colheita de amostras. Quando não podia realizar todas estas funções, recorria a pessoal não especializado que o próprio tinha que formar e preparar, despendendo tempo e recursos, em detrimento do atendimento e prestação de serviços Médico-Veterinários mais especializados. A nível Europeu o Curso de Enfermagem Veterinária foi contemplado no tratado de Bolonha e a nível internacional, vários países consideravam, desde há muito, que a Enfermagem Veterinária, a par com os colégios de especialidade, seriam a chave para o desenvolvimento da boa prática dos cuidados veterinários e para a evolução da Medicina Veterinária, permitindo ao Médico Veterinário uma dedicação maior à clínica da especialidade, concentrando-se exclusivamente no diagnóstico, tratamento e na cirurgia em si. A 11 de Outubro de 2012, a Enfermagem Veterinária no Reino Unido fazia 50 anos, celebrados com pompa e circunstância na Casa dos Comuns, enquanto em Portugal, esta jovem profissão, a dar os seus primeiros passos, era vista como uma ameaça por muitos, incluindo Médicos Veterinários, Ordem dos Enfermeiros, entre outros, e nem sequer era ainda reconhecida nos Centros de Emprego. Contudo, foi o advento desta nova profissão que levou ao surgimento da equipa Médico-veterinária que pressupõe a existência de vários profissionais, com competências distintas mas que devem interagir em prol do objetivo comum da prestação de cuidados especializados de saúde e de bem-estar ao paciente animal. Em 2014, o curso de Enfermagem Veterinária da Escola Superior Agrária de Viseu obteve a acreditação europeia pela ACOVENE e Portugal junta-se assim à Bélgica, Holanda, Itália, Noruega e Reino Unido, os países onde são atualmente acreditados cursos de Enfermagem Veterinária pela ACOVENE.

Interface virtual com sensor 3D para interação de pessoas acamadas

A interação homem-máquina tem evoluído significativamente nos últimos anos, a ponto de permitir desenvolver soluções adequadas para apoio a pessoas que possuem um certo tipo de limitação física ou cognitiva. O desenvolvimento de técnicas naturais e intuitivas de interação, as chamadas Natural User Interface (NUI), permitem, hoje, que pessoas que estejam acamadas e/ou com incapacidade motora possam executar um conjunto de ações por intermédio de gestos, aumentando assim a sua qualidade de vida. A solução implementada neste projecto é baseada em processamento de imagem e visão por computador através do sensor 3D Kinect e consiste numa interface natural para o desenvolvimento de uma aplicação que reconheça gestos efetuados por uma mão humana. Os gestos identificados pela aplicação acionam um conjunto de ações adequados a uma pessoa acamada, como, por exemplo, acionar a emergência, ligar ou desligar a TV ou controlar a inclinação da cama. O processo de desenvolvimento deste projeto implicou várias etapas. Inicialmente houve um trabalho intenso de investigação sobre as técnicas e tecnologias consideradas importantes para a realização do trabalho - a etapa de investigação, a qual acompanhou praticamente todo o processo. A segunda etapa consistiu na configuração do sistema ao nível do hardware e do software. Após a configuração do sistema, obtiveram-se os primeiros dados do sensor 3D Kinect, os quais foram convertidos num formato mais apropriado ao seu posterior tratamento. A segmentação da mão permitiu posteriormente o reconhecimento de gestos através da técnica de matching para os seis gestos implementados. Os resultados obtidos são satisfatórios, tendo-se contabilizado cerca de 96% de resultados válidos. A área da saúde e bem-estar tem necessidade de aplicações que melhorem a qualidade de vida de pessoas acamadas, nesse sentido, o protótipo desenvolvido faz todo o sentido na sociedade actual, onde se verifica o envelhecimento da população.