Effect of blocking Rac1 expression in cholangiocarcinoma QBC939 cells

Cholangiocarcinomas (CCs) are malignant tumors that originate from epithelial cells lining the biliary tree and gallbladder. Ras correlative C3 creotoxin substrate 1 (Rac1), a small guanosine triphosphatase, is a critical mediator of various aspects of endothelial cell functions. The objective of the present investigation was to study the effect of blocking Rac1 expression in CCs. Seventy-four extrahepatic CC (ECC) specimens and matched adjacent normal mucosa were obtained from the Department of Pathology, Inner Mongolia Medicine Hospital, between 2007 and 2009. Our results showed that the expression of Rac1 was significantly higher (53.12%) in tumor tissues than in normal tissues. Western blotting data indicated a significant reduction in Rac1-miRNA cell protein levels. Rac1-miRNA cell growth rate was significantly different at 24, 48, and 72 h after transfection. Flow cytometry analysis showed that Rac1-miRNA cells undergo apoptosis more effectively than control QBC939 cells. Blocking Rac1 expression by RNAi effectively inhibits the growth of CCs. miRNA silencing of the Rac1 gene suppresses proliferation and induces apoptosis of QBC939 cells. These results suggest that Rac1 may be a new gene therapy target for CC. Blocking Rac1 expression in CC cells induces apoptosis of these tumor cells and may thus represent a new therapeutic approach.

De insulis

Invokaatio: Q.D.B.V!

Skhedárion quaternionis meletematum, e venerando et augusto sapientiae archivo erutorum

Invokaatio: Sun to Theo mono athanato.

Natura prudentiae

Variantti A.

De majestate

Variantti A.

Super eloquentia

Invokaatio: Α.Ω.

Vindicias Providentiae divinae contra exceptiones deistarum, continens

Invokaatio: D.D.

De nova stella mobili

Painovuosi nimekkeestä.

Methodus inveniendi tempus verum ex observatis aequalibus diversarum stellarum altitudinibus 1

Dedikaatio: Carl Gustaf Wünsch [ruots. pr.], Adam Reinh. Tammelander [ruots. pr.], Anna Christina Wünsch [ruots. pr.].

De quadratura parabolae

Arkit: A-B4 C3. - S. [22] tyhjä.

De exceptione metus

Invokaatio: A.G.D.

Gene transfer of Chlorella vulgaris n-3 fatty acid desaturase optimizes the fatty acid composition of human breast cancer cells

Chlorella vulgaris has the gene of n-3 fatty acid desaturase (CvFad3), which can synthesize the precursor of n-3 polyunsaturated fatty acids (PUFAs) or convert n-6 to n-3 PUFAs. The objective of the present study was to examine whether the CvFad3 gene from C. vulgaris can be functionally and efficiently expressed in human breast cancer cells and whether its expression can exert a significant effect on cell fatty acid composition. We inserted the CvFad3 gene into the plasmid pEGFP-C3 to construct the eukaryotic expression vector pEGFP-C3-n-3 and to express the n-3 Fad gene in human breast cancer cells (MCF-7 cells). Transfection of MCF-7 cells with the recombinant vector resulted in a high expression of n-3 fatty acid desaturase. Lipid analysis indicated that the ratio of n-6/n-3 PUFAs was decreased from 6:1 in the control cells to about 1:1 in the cells expressing the n-3 fatty acid desaturase. Accordingly, the CvFad3 gene significantly decreased the ratio of n-6/n-3 PUFAs of the MCF-7 cell membrane. The expression of the CvFad3 gene can decrease cell proliferation and promote cell apoptosis. This study demonstrates that the CvFad3 gene can dramatically balance the ratio of n-6/n-3 PUFAs and may provide an effective approach to the modification of the fatty acid composition of mammalian cells, also providing a basis for potential applications of its transfer in experimental and clinical settings.

Dissfrtatio [!] philologica ... fidem Jobi ex cap. XIX:25, 26, 27. Considerans, qvam ex decrete celeberrimi consistorii Acad. Aboens. adprobante ampliss. facult. phil. publico bonorum examini sistit mag. Matthias Hallenius, facult. theol. adjunctus & pastor in Lundå, respondente Gabriele Tålpo, Aboensi, ad diem Deo volente, XXVII Julii, anni MDCCXLI. In auditorio maximo, horis ante meridiem solitis

Invokaatio: In nomine Jesu!

De caussa elasticitatis

Invokaatio: Q.F.F.S.

De insulis

Invokaatio: Q.D.B.V.