Wirkungsbezogene Detektion von toxischen Substanzen in Fisch und Umwelt

The detection of unknown and the quantification of known toxic substances is a challenge for the modern chemical and biological analysis. Effect-related in vitro methods, e.g. AH-receptor or estrogene-receptor mediated, offer some advantages compared to the classical chemical analysis. It could be shown that two cell lines exhibit different sensitivities to 2,3,7,8-tetrachlorodibenzodioxin (TCDD), although the cells contain the identical dioxin-receptor. The application and limitations of these methods in envi-ronmental analysis are discussed.

Horizontale Trennblätter in Trawls zur Verringerung von Discards in der Ostsee

A primary objective of the Common Fishery Policy of the European Union is the reduction of discards and unwanted by-catches in the fishery. In principle this could be achieved if the catching methods were optimised for this. Still high numbers of undersized flatfish are caught in the bottom trawls. Although EU regulations make the use of the BACOMA codend mandatory in the Baltic Sea cod areable to escape through square mesh escape window of the BACOMA net the whereas flatfish still remain in the cod-end. Gear experiments have been carried out with the aim to better separate cod from the flatfish fraction already when entering the rear belly, making use of the natural behaviour of the fish, i. e. the preferred swimming distance from the bottom of the net in the funnel. As cod have a natural tendency to keep a relativly great distance from the bottom, flatfish tend to stay close to it. It was attempted to separate both fractions by splitting the funnel into an upper and lower part with a horizontal panel. This wastested for two different nets, a cod trawl to separate cod from flatfish, and an eel-trawl to separate cod and flatfish from eel. Cod and flatfish separation is best at a panel distance of 50 cm from the bottom. Thus, 74 % of the cod were found in the upper panel, whereas 75 % of the flounder were in the lower section. A separation of eel from cod was however not possible, since eel tend to rise to the upper part of the net, together with cod.

Die Anwendung molekulargenetischer Verfahren in der fischereiwissenschaftlichen Forschung - Trennung von Fischpopulationen

The discrimination of stocks and separate reproductive units within fish species to facilitate fisheries management based on biological data has always been a challenge to fisheries biologists. We describe the use of three different molecular genetic techniques to detect genetic differences between stocks and closely related species. Direct sequencing of the mitochondrial ND3 gene describes the relationship between different aquaculture strains and natural populations of rainbow trout and revealed genetic homogeneity within the hatchery strains. Microsatellite analyses were used to explore the differences between redfish species from the genus Sebastes and to verify populations structure within S. mentella and S. marinus. This lead to an un equivocal discrimination of the species and an indication of populations structure within those species in the North Atlantic. The Amplified Fragment Length Polymorphisum (AFLP) methodology revealed genetic differences between Baltic and North Sea dap (Limanda limanda)and a possible population structure within the North Sea.

Der PAH-Metabolit 1-Hydroxypyren in der Galle von Klieschen (Limanda limanda) aus Nord- und Ostsee

Due to their carcinogenic properties, polycyclic aromatic hydrocarbons (PAH) are important environmental contaminants which may lead to increased levels of neoplastic aberrations in fish liver. To measure the PAH contamination of fish, concentrations of PAH metabolites were determined in the bile. The main metabolite 1-hydroxypyrene was determined in concentrations from < 0.8 to 189 ng/ml in bile of dab caught in the North Sea and the Baltic Sea re-spectively. The concentrations are comparable to levels of 1-hydroxypyrene measured in cod and herring from the Baltic Sea. The highest concentrations in dab bile were measured in fish from the inner German Bight and the Kiel Bight.

Die Qualitäts-Index-Methode (QIM), ein verlässliches Instrument zur sensorischen Bewertung der Frische von Fisch

Fish research institutes in Europe have made considerable effort in developing rapid, objective sensory methods for evaluation of fish freshness. The Quality Index Method(QIM) has been recommended for a European initiative regarding standardisation and harmonisation of sensory evaluation of fish. QIM-schemes have been developed for various common European fish species. Research has now provided the industry with a convenient, objective and powerful tool for measuring freshness of fish kept in ice Further research is needed to evaluate the applicability of QIM for fish handled, stored and processed under different conditions. However, for progress and development of QIM it is now very important that the fish sector implements QIM in fish auctions and the quality management system of the fish processing plants.

Einfluss von Reifung und technologischen Behandlungen auf die Proteine des Rogens von Regenbogenforellen (Oncorhynchus mykiss)

The use of differential scanning calorimetry for investigating the yolk proteins of the roe of rainbow trout allows to monitor the influence of maturation and vitellogenesis which modifies the DSC curves with regard to transition temperature and enthalpy. Two endothermic peaks become more and more pronounced with increasing maturation of the roe. However, it is still not known which protein fraction is represented by each of the peaks. DSC curves of yolk protein depend on fish species. They are also influenced by technological treatments. Further investigation is necessary to discover whether or not yolk proteins of other fish species are influenced in the same manner by maturation and vitellogenesis and which protein fraction represent the individual peaks.

Untersuchungen zur Aufzucht von Barramundi

Lates calcarifer (Barramundi) ist ein naher Verwandter des bei uns inzwischen seit Jahren bekannten und auf vielen Wochenmärkten zu findenden Viktoriabarsches(Lates niloticus), dem er auch im Aussehen ähnelt. Das Verbreitungsgebiet dieses euryhalinen Fisches sind die Küstenregionen mitsamt ihren Flußgebietssystemen des nördlichen Indischen und des westlichen tropischen Pazifischen Ozeans. Bei einer derartigen weiten Verbreitung ist es nicht verwunderlich, daß sich viele Bestände ausgebildet haben, die durchaus unterschiedliche Leistungen z. B. in Hinblick auf die Reproduktionsbiologie zeigen.

ICES-Arbeitsgruppentreffen zum "Lebenszyklus und Fischerei von Nordseegarnelen" (WGCRAN)

Two years after their last meeting, scientists from North Sea neighbouring countries working on aspects of brown shrimp biology and fisheries gathered in Oostende, Belgium, to exchange information and results of their research. The group was established ten years ago by the International Council of the Exploration of the Sea (ICES)during the Warnemünde Annual Science Conference. Data on brown shrimp landings, fishing effort and resulting landings per unit effort were compiled. For the first time a computer model was demonstrated simulating the life cycle of C. crangon on basis of experimental and field data available. It will provide a means for testing different possible scenarios and their effect on the brown shrimp stocks. Catch predictions are not possible by this, as no stock assessments can yet be achieved for brown shrimp, and a number of topics have to be addressed by further research programmes. However, an approach of estimating the level of landings on basis of preceding climatological and hydrographical data seemed promising. Furthermore selectivity experiments and electric gear types were reported giving reason to assume, that progress is possible in the further reduction of bycatches. The assembling of already existing data in various countries and their evaluation was recommended besides the pursuing of the mentioned fields of research and proper reporting of EU log book data by all countries.