New insights into the karyotypic relationships of Chinese muntjac (Muntiacus reevesi), forest musk deer (Moschus berezovskii) and gayal (Bos frontalis)

To investigate the karyotypic relationships between Chinese muntjac (Muntiacus reevesi), forest musk deer (Moschus berezovskii) and gayal (Bos frontalis), a complete set of Chinese muntjac chromosome-specific painting probes has been assigned to G-banded chromosomes of these three species. Sixteen autosomal probes (i.e. 6-10, 12-22) of the Chinese muntjac each delineated one pair of conserved segments in the forest musk deer and gayal, respectively. The remaining six autosomal probes (1-5, and 11) each delineated two to five pairs of conserved segments. In total, the 22 autosomal painting probes of Chinese muntjac delineated 33 and 34 conserved chromosomal segments in the genomes of forest musk deer and gayal, respectively. The combined analysis of comparative chromosome painting and G-band comparison reveals that most interspecific homologous segments show a high degree of conservation in G-banding patterns. Eleven chromosome fissions and five chromosome fusions differentiate the karyotypes of Chinese muntjac and forest musk deer; twelve chromosome fissions and six fusions are required to convert the Chinese muntjac karyotype to that of gayal; one chromosome fission and one fusion separate the forest musk deer and gayal. The musk deer has retained a highly conserved karyotype that closely resembles the proposed ancestral pecoran karyotype but shares none of the rearrangements characteristic for the Cervidae and Bovidae. Our results substantiate that chromosomes 1-5 and 11 of Chinese muntjac originated through exclusive centromere-to-telomere fusions of ancestral acrocentric chromosomes. Copyright (C) 2005 S. Karger AG, Basel.

An investigation of the kinetics of CO2 uptake by a synthetic calcium based sorbent

This study examines the kinetics of carbonation by CO2 at temperatures of ca. 750 °C of a synthetic sorbent composed of 15 wt% mayenite (Ca12Al14O33) and CaO, designated HA-85-850, and draws comparisons with the carbonation of a calcined limestone. In-situ XRD has verified the inertness of mayenite, which neither interacts with the active CaO nor does it significantly alter the CaO carbonation–calcination equilibrium. An overlapping grain model was developed to predict the rate and extent of carbonation of HA-85-850 and limestone. In the model, the initial microstructure of the sorbent was defined by a discretised grain size distribution, assuming spherical grains. The initial input to the model – the size distribution of grains – was a fitted parameter, which was in good agreement with measurements made with mercury porosimetry and by the analysis of SEM images of sectioned particles. It was found that the randomly overlapping spherical grain assumption offered great simplicity to the model, despite its approximation to the actual porous structure within a particle. The model was able to predict the performance of the materials well and, particularly, was able to account for changes in rate and extent of reaction as the structure evolved after various numbers of cycles of calcination and carbonation.

一种新的小鼠被毛突变基因定位

分别尝试用RAPD法和重组率测定法对所发现的被毛突变进行基因定位。结果为所选用的100个10bp RAPD引物中,87个获得了扩增产物,由于所获得的产物与小鼠表型间缺乏相关性,利用RAPD法未能将该基因定位到染色体上。以分布于小鼠11条染色体上的Idh1、Car2、Mup1、Pgm1、Hbb、Es10、Es1、Mod1、Gdc1、Ce2、Es3等生化基因位点为遗传标记,对分别与CBA/N和C57BL/6小鼠互交-回交所获得的F2代进行两点测试,结果表明该突变与Es3连锁,位于小鼠第11号染色体上。

Rapid Electron Beam Reacted Tantalum/Titanium Bilayers on Silicon : Semiconductors and Semiconductor Devices

The electrical and structural characteristics of tantalum-titanium bilayers on silicon reacted by electron beam heating have been investigated over a wide range of temperature and time conditions. The reacted layers exhibit low sheet resistance and stable electrical characteristics up to at least 1100℃. Titanium starts reacting from 750℃ onwards for 100 milliseconds reaction times whereas tantalum starts reacting only above 900℃ for such short reaction times. RBS results confirm that silicon is the major diffusing species and there is no evidence for the formation of ternary silicides. Reactions have also been explored on millisecond time scales by non-isothermal heating.

复合养殖系统中浮游动物种类丰度及其影响因素

利用人工湿地和养殖塘构建了复合养殖系统,通过设置不同塘间水质差异,调查了斑点叉尾鮰鱼鱼种培育段和成鱼养殖段不同梯度下的浮游动物种类丰度,并探讨了种类丰度与环境因子的关系.结果表明,2养殖阶段的循环塘中,轮虫的种类丰度随养殖密度的增加而升高,浮游甲壳动物随养殖密度的增加而降低.成鱼养殖阶段对照塘中轮虫的种类丰度高于循环塘P1、P2;枝角类和桡足类的丰度则低于P1、P2;高养殖密度塘P3中,3种形态浮游动物种类丰度均低于对照塘.15种环境因子的主成分分析以及种类丰度与环境因子的冗余度分析结果表明,影响浮游动物

实验室条件下沙埋对人工藻结皮生物量、光合活性和胞外多糖的影响

土壤蓝藻结皮在干旱和半干旱地区广泛分布.沙埋是生长在流动沙丘表面的植物面临的重要的环境因子.实验研究了温室条件下干燥沙子不同掩埋时间(0,5,10,15,20,30天)和深度(0,0.2,0.5,1,2cm)对人工藻结皮生物量、叶绿素荧光活性和胞外多糖的影响.结果表明:大体上随着沙埋时间的延长和深度的增加人工藻结皮的F_v/F_m值和胞外多糖含量逐渐降低,但是在20天和30天沙埋处理之间,两者在不同沙埋深度均不存在显著性差异;生物量的降低出现在沙埋处理20天和30天,在不同的沙埋深度这2种处理时间之间差异

Microsized piston-cylinder pneumatic and hydraulic actuators fabricated by lithography

Future microrobotic applications require actuators that can generate a high actuation force in a limited volume. Up to now, little research has been performed on the development of pneumatic or hydraulic microactuators, although they offer great prospects in achieving high force densities. In addition, large actuation strokes and high actuation speeds can be achieved by these actuators. This paper describes a fabrication process for piston-cylinder pneumatic and hydraulic actuators based on etching techniques, UV-definable polymers, and low-temperature bonding. Prototype actuators with a piston area of 0.15 mm2 have been fabricated in order to validate the production process. These actuators achieve actuation forces of more than 0.1 N and strokes of 750 μm using pressurized air or water as driving fluid. © 2009 IEEE.