2,4-Lutidine-1-oxide complexes of lanthanide perchlorates

2,4-Lutidine-1-oxide (2,4-LutO) complexes of lanthanide perchlorates of the formulae Ln2(2,4-LutO)13(ClO4)6 (Ln = Pr and Nd) and Ln2(2,4-LutO)15 (ClO4)6 (Ln = La, Tb, Dy, Ho and Yb) have been prepared and characterised by chemical analysis, IR, NMR, conductance and electronic spectral data. Proton NMR data along with the IR data show that the ligand coordinates to the metal ion through the oxygen. Conductance data of the complexes in acetone and nitrobenzene indicate that the perchlorate is not coordinated to the metal ion.

Bonding isomerism in the chelate-exchange reactions of isonitroso-β-keto-imino isomeric nickel(ii) mixed ligand complexes

A novel chelate exchange reaction, leading to the formation of a series of N-alkyl substituent dependent mixed ligand isomeric complexes of the type Ni(R-AB)(AC') and Ni(R-AC)(AB') (Figure 1) are discussed. Here, AB and AC denote two different N-bonded isonitroso-β-keto-imino ligand moieties, while AB' and AC' are the corresponding O-bonded ligand moieties and R is an N-alkyl substituent. The isomeric complexes are suggested to be monomeric, neutral and diamagnetic with an asymmetric square planar geometry. The bonding isomerism of the isonitroso group in these complexes is discussed on the basis of the infrared and proton magnetic resonance spectral studies. A probable mechanism for the preparative route is also proposed.

Noise minimization in RC-active filters using generalized impedance converters

A procedure has been given for minimizing the total output noise of a Generalized Impedance Converter (GIC), subject to constraints dictated by signal handling capability of the Operational Amplifiers and ease of microcircuit fabrication. The noise reduction is achieved only by the adjustment of RC elements of the GIC, and the total output noise after optimization in the example cited is close to the theoretical lower limit. The output noise of a higher-order filter can be reduced by RC-optimizing the individual GIC's of the active realization. Experimental results on a 20–24 kHz channel bank band-pass filter demonstrate the effectiveness of the above procedure.

Chemical approaches to penicillin allergy—V. Nature of reaction between rabbit antigen (CRP-penicillin conjugate) and rabbit antipenicillin antibody

The availability of an electrophoretically homogeneous rabbit penicillin carrier receptor protein (CRP) and rabbit antipenicillin antibody afforded an idealin vitro system to calculate the thermodynamic parameters of the binding of14C benzyl penicillin CRP conjugate (antigen) to the purified rabbit antipenicillin antibody. The thermodynamic parameters of this antigen-antibody reaction has been studied by radio-active assay method by using millipore filter. Equilibrium constant (K) of this reaction has been found to be 2·853×109M−2 and corresponding free energy (ΔG) at 4°C and 37°C has been calculated to be −12·02 and −13·5 kcal/mole, enthalpy (ΔH) and entropy (ΔS) has been found to be 361 kcal/mole and +30 eu/mole respectively. Competitive binding studies of CRP-analogue conjugates with the divalent rabbit antibody has been carried out in the presence of14C-penicilloyl CRP. It was found that 7-deoxy penicillin-CRP complex and 6-amino penicilloyl CRP conjugate binds to the antibody with energies stronger than that with the14C-penicilloyl CRP. All the other analogue conjugates are much weaker in interfering with the binding of the penicilloyl CRP with the antibody. The conjugate of methicillin,o-nitro benzyl penicillin and ticarcillin with CRP do not materially interfere in the process.

Identifying N60D mutation in omega subunit of Escherichia coli RNA polymerase by bottom-up proteomic approach

Escherichia coli RNA polymerase is a multi-subunit enzyme containing alpha(2)beta beta'omega sigma, which transcribes DNA template to intermediate RNA product in a sequence specific manner. Although most of the subunits are essential for its function, the smallest subunit omega (average molecular mass similar to 10,105 Da) can be deleted without affecting bacterial growth. Creating a mutant of the omega subunit can aid in improving the understanding of its role. Sequencing of rpoZ gene that codes for omega subunit from a mutant variant suggested a substitution mutation at position 60 of the protein: asparagine (N) -> aspartic acid (D). This mutation was verified at the protein level by following a typical mass spectrometry (MS) based bottom-up proteomic approach. Characterization of in-gel trypsin digested samples by reverse phase liquid chromatography (LC) coupled to electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) enabled in ascertaining this mutation. Electron transfer dissociation (ETD) of triply charged (M + 3H)(3+)] tryptic peptides (residues 53-67]), EIEEGLINNQILDVR from wild-type and EIEEGLIDNQILDVR from mutant, facilitated in unambiguously determining the site of mutation at residue 60.

Corporate Brand Repositioning with CSR as the Differentiating Factor: A Study on Consumer Perceptions

Purpose – This research paper studies how the strategy of repositioning enables marketers to communicate CSR as their brand’s differentiating factor. It aims at understanding how consumer perceptions can be managed to generate brand value through corporate brand repositioning when CSR is the differentiating factor. The purpose of this paper is to answer the following research question: How can consumer perceptions be managed to generate brand value through corporate brand repositioning when CSR is the differentiating factor? The two research objectives were: 1. to build a model, which describes the different components of consumer perceptions involved in generation of brand value through repositioning when CSR is the differentiating factor, 2. to identify the most critical components in the context of the case company, IKEA for generation of brand value during the process of corporate brand repositioning Design/methodology/approach – This paper is based on the literature review covering the logic of brand value generation, repositioning strategy and consumer perceptions connected to CSR activities. A key concept of the positioning theory, the brand’s differentiating factor, was explored. Previous studies have concluded that desirability of the differentiating factor largely determines the level of brand value-creation for the target customers. The criterion of desirability is based on three dimensions: relevance, distinctiveness and believability. A model was built in terms of these desirability dimensions. This paper takes a case study approach where the predefined theoretical framework is tested using IKEA as the case company. When developing insights on the multifaceted nature of brand perceptions, personal interviews and individual probing are vital. They enable the interviewees to reflect on their feelings and perceptions with their own words. This is why the data collection was based on means-end type of questioning. Qualitative interviews were conducted with 12 consumers. Findings – The paper highlights five critical components that may determine whether IKEA will fail in its repositioning efforts. The majority of the critical components involved believability perceptions. Hence, according to the findings, establishing credibility and trustworthiness for the brand in the context of CSR seems primary. The most critical components identified of the believability aspect were: providing proof of responsible codes of conduct via conducting specific and concrete CSR actions, connecting the company’s products and the social cause, and building a linkage between the initial and new positioning while also weakening the old positioning. Originality/value – Marketers’ obligation is to prepare the company for future demands. Companies all over the globe have recognized the durable trend of responsibility and sustainability. Consumer´s worry about the environmental and social impact of modern lifestyles is growing. This is why Corporate Social Responsibility (CSR) provides brands an important source of differentiation and strength in the future. The strategy of repositioning enables marketers to communicate CSR as their brand’s differentiating factor. This study aimed at understanding how consumer perceptions can be managed to generate brand value through corporate brand repositioning when CSR is the differentiating factor.

Genome-wide expression profiling identifies deregulated miRNAs in malignant astrocytoma

Malignant astrocytoma includes anaplastic astrocytoma (grade III) and glioblastoma (grade IV). Among them, glioblastoma is the most common primary brain tumor with dismal responses to all therapeutic modalities. We performed a large-scale, genome-wide microRNA (miRNA) (n=756) expression profiling of 26 glioblastoma, 13 anaplastic astrocytoma and 7 normal brain samples with an aim to find deregulated miRNA in malignant astrocytoma. We identified several differentially regulated miRNAs between these groups, which could differentiate glioma grades and normal brain as recognized by PCA. More importantly, we identified a most discriminatory 23-miRNA expression signature, by using PAM, which precisely distinguished glioblastoma from anaplastic astrocytoma with an accuracy of 95%. The differential expression pattern of nine miRNAs was further validated by real-time RT-PCR on an independent set of malignant astrocytomas (n-72) and normal samples (n=7). Inhibition of two glioblastoma-upregulated miRNAs (miR-21 and miR-23a) and exogenous overexpression of two glioblastoma-downregulated miRNAs (miR-218 and miR-219-5p) resulted in reduced soft agar colony formation but showed varying effects on cell proliferation and chemosensitivity. Thus we have identified the miRNA expression signature for malignant astrocytoma, in particular glioblastoma, and showed the miRNA involvement and their importance in astrocytoma development. Modern Pathology (2010) 23, 1404-1417; doi:10.1038/modpathol.2010.135; published online 13 August 2010

Regulation and Function of GATA Transcription Factors in Adrenocortical Tumors and Granulosa Cells

Transcription factors play a key role in tumor development, in which dysfunction of genes regulating tissue growth and differentiation is a central phenomenon. The GATA family of transcription factors consists of six members that bind to a consensus DNA sequence (A/T)GATA(A/G) in gene promoters and enhancers. The two GATA factors expressed in the adrenal cortex are GATA-4 and GATA-6. In both mice and humans, GATA-4 can be detected only during the fetal period, whereas GATA-6 expression is abundant both throughout development and in the adult. It is already established that GATA factors are important in both normal development and tumorigenesis of several endocrine organs, and expression of GATA-4 and GATA-6 is detected in adrenocortical tumors. The aim of this study was to elucidate the function of these factors in adrenocortical tumor growth. In embryonal development, the adrenocortical cells arise and differentiate from a common pool with gonadal steroidogenic cells, the urogenital ridge. As the adult adrenal cortex undergoes constant renewal, it is hypothesized that undifferentiated adrenocortical progenitor cells reside adjacent to the adrenal capsule and give rise to daughter cells that differentiate and migrate centripetally. A diverse array of hormones controls the differentiation, growth and survival of steroidogenic cells in the adrenal gland and the gonads. Factors such as luteinizing hormone and inhibins, traditionally associated with gonadal steroidogenic cells, can also influence the function of adrenocortical cells in physiological and pathophysiological states. Certain inbred strains of mice develop subcapsular adrenocortical tumors in response to gonadectomy. In this study, we found that these tumors express GATA-4, normally absent from the adult adrenal cortex, while GATA-6 expression is downregulated. Gonadal markers such as luteinizing hormone receptor, anti-Müllerian hormone and P450c17 are also expressed in the neoplastic cells, and the tumors produce gonadal hormones. The tumor cells have lost the expression of melanocortin-2 receptor and the CYP enzymes necessary for the synthesis of corticosterone and aldosterone. By way of xenograft studies utilizing NU/J nude mice, we confirmed that chronic gonadotropin elevation is sufficient to induce adrenocortical tumorigenesis in susceptible inbred strains. Collectively, these studies suggest that subcapsular adrenocortical progenitor cells can, under certain conditions, adopt a gonadal fate. We studied the molecular mechanisms involved in gene regulation in endocrine cells in order to elucidate the role of GATA factors in endocrine tissues. Ovarian granulosa cells express both GATA-4 and GATA-6, and the TGF-β signaling pathway is active in these cells. Inhibin-α is both a target gene for, and an atypical or antagonistic member of the TGF-β growth factor superfamily. In this study, we show that GATA-4 is required for TGF-β-mediated inhibin-α promoter activation in granulosa cells, and that GATA-4 physically interacts with Smad3, a TGF-β downstream protein. Apart from the regulation of steroidogenesis and other events in normal tissues, TGF-β signaling is implicated in tumors of multiple organs, including the adrenal cortex. Another signaling pathway found often to be aberrantly active in adrenocortical tumors is the Wnt pathway. As both of these pathways regulate the expression of inhibin-α, a transcriptional target for GATA-4 and GATA-6, we wanted to investigate whether GATA factors are associated with the components of these signaling cascades in human adrenocortical tumors. We found that the expression of Wnt co-receptors LRP5 and LRP6, Smad3, GATA-6 and SF-1 was diminished in adrenocortical carcinomas with poor outcome. All of these factors drive inhibin-α expression, and their expression in adrenocortical tumors correlated with that of inhibin-α. The results support a tumor suppressor role previously suggested for inhibin-α in the mouse adrenal cortex, and offer putative pathways associated with adrenocortical tumor aggressiveness. Unraveling the role of GATA factors and associated molecules in human and mouse adrenocortical tumors could ultimately contribute to the development of diagnostic tools and future therapies for these diseases.

Switching studies and the effect of irradiation on ferroelectric properties of TAAP and DTAAP

Polarization switching processes in TAAP and DTAAP have been studied by the Merz method. The switching process in DTAAP is slower than in TAAP. The temperature dependence of switching time indicates that the crystal might contain groups of domain nuclei with different activation energies. X-ray irradiation causes an increase in the threshold field below which switching could not occur and decrease in the mobility of domain walls. Irradiation decreases the peak value of dielectric constant, Tc and increases the value of coercive field. Domain structure studies on TAAP crystals have shown that the crystals grow as both predominantly single domain and multi domains, depending on which the internal bias increases or remains unaffected upon irradiation.

Museo yhteiskunnan peilinä? - pääkaupunkiseudulle perustetut kulttuurihistorialliset museot 1875-2010

Tässä pro gradu –tutkielmassa tarkastellaan museoita osana yhteiskunnan historiakulttuuria; niitä tapoja joiden pohjalta käsityksiä menneestä tuotetaan ja käytetään. Aihetta lähestytään kysymällä millaisia kulttuurihistoriallisia museoita pääkaupunkiseudulle on perustettu ja millaiset teemat eivät ole olleet museon arvoisia. Tutkielmassa selvitetään onko pääkaupunkiseudulle perustetuissa kulttuurihistoriallisissa museoissa havaittavissa ajallista aaltoa sen suhteen millaisille aiheille on perustettu oma museo. Lopuksi pohditaan esimerkkitapausten valossa sitä, miten erilaisia teemoja edustavien museoiden perustamistarvetta on perusteltu omana aikanaan. Etsimällä vastauksia tutkimuskysymyksiin pyritään samalla hahmotetaan sitä, millaista yhteiskunnallista historiakulttuuria museot ovat olleet osa. Työn ensimmäisessä vaiheessa kartoitettiin millaisia kulttuurihistoriallisia museoita pääkaupunkiseudulla on ollut. Museoviraston ja Suomen museoliiton aineistojen avulla on listattu kooste vuosien 1875-2010 välillä pääkaupunkiseudulla olleista museoista. Museoiden perustamiseen ja museokentältä katoamiseen liittyvää koostetta on täydennetty Helsingin matkailuyhdistyksen julkaisujen tiedoilla sekä museoiden omilla tiedonannoilla. Tutkielman museot on luokiteltu edustamiensa teemojen mukaan. Tarkoituksena on ollut hahmotella miten pääkaupunkiseudun kulttuurihistoriallisten museoiden kenttä on muuttunut 135 vuoden aikana. Työn toisessa vaiheessa tarkasteltiin neljän esimerkkitapauksen kautta museokentän ajallista muutosta ja sitä millaisia perusteluita museoiden perustamiselle on annettu sekä millaista keskustelua museon perustamisesta on omana aikanaan käyty. Esimerkkitapausten tarkastelun lähteinä käytettiin arkistomateriaalia, aikakauden lehdissä museoista käytyä keskustelua ja museoista kirjoitettuja historiikkeja. Pääkaupunkiseudun kulttuurihistoriallisissa museoissa on tutkielman mukaan havaittavissa löyhä ajallinen muutos. Kulttuurihistoriallisten museoiden edustamat historian kertomukset ovat muuttuneet ja pirstaloituneet 135 vuoden aikana virallisesta, kansallisesta historian kertomuksesta kohti useita pienempiä historian kertomuksia ja erilaisten ryhmien omaa historiaa. 1800-1900-lukujen taitteessa perustettiin pääosin valtiollisia ja instituutioiden historiaan keskittyneitä museoita sekä suurmiehille omistettuja henkilöhistoriallisia museoita. Valtiolliset ja henkilöhistorialliset teemat näkyvät museoissa läpi koko tutkielman tarkasteluajanjakson. Kansallisen historian katse suuntasi paikalliseen 1900-luvun alussa ja 1980-luvulla, jolloin perustettiin erityisen paljon paikallishistoriallisia ja alueellisia museoita. 1970-1990-luvuilla museokenttää hallitsivat monet tekniikkaan, teollisuuteen ja yrityshistoriaan keskittyneet museot. Tultaessa 2000-luvulle museot edustivat lapsiin, nuoriin, koulutukseen ja monikulttuurisuuteen liittyviä teemoja. Tutkielman toisen vaiheen esimerkkitapausten tarkastelu osoitti, että erilaisia teemoja edustavien museoiden perustamistarvetta perustellaan hyvin samankaltaisesti. Niin 1800-luvun lopussa kuin 1900-luvun lopussa museoiden tarvetta perusteltiin kasvatus- ja sivistystyöllä ja aihealueen arvostuksen kasvulla. Museon perustamisen koettiin osoittavan Suomen kuuluvan osaksi länsimaisia sivistysyhteiskuntia. Museoiden perustamisen taustalla on usein ollut asiaa ajanut yhdistys. Kuitenkin museohanke on ollut vuosikausien projekti ja konkretisoitunut usein vasta kun museon tiloihin ja talouteen liittyvät kysymykset on saatu kuntoon. Tutkielman loppupäätelmien myötä hahmottui monia historiantutkimuksellisen lisätarkastelun arvoisia aiheita museoista osana yhteiskunnan historiakulttuuria. Avainsanat – Nyckelord – Keywords kulttuurihistorialliset museot, historiakulttuuri, historiankäyttö, yhteiskunta