Meta-analysis of estrogen response in MCF-7 distinguishes early target genes involved in signaling and cell proliferation from later target genes involved in cell cycle and DNA repair.

ABSTRACT: BACKGROUND: Many studies have been published outlining the global effects of 17 beta-estradiol (E2) on gene expression in human epithelial breast cancer derived MCF-7 cells. These studies show large variation in results, reporting between ~100 and ~1500 genes regulated by E2, with poor overlap. RESULTS: We performed a meta-analysis of these expression studies, using the Rank product method to obtain a more accurate and stable list of the differentially expressed genes, and of pathways regulated by E2. We analyzed 9 time-series data sets, concentrating on response at 3-4 hrs (early) and at 24 hrs (late). We found >1000 statistically significant probe sets after correction for multiple testing at 3-4 hrs, and >2000 significant probe sets at 24 hrs. Differentially expressed genes were examined by pathway analysis. This revealed 15 early response pathways, mostly related to cell signaling and proliferation, and 20 late response pathways, mostly related to breast cancer, cell division, DNA repair and recombination. CONCLUSIONS: Our results show that meta-analysis identified more differentially expressed genes than the individual studies, and that these genes act together in networks. These results provide new insight into E2 regulated mechanisms, especially in the context of breast cancer.

Customer Focus Newsletter, March-April, 2010, Vol. 7, no.2

A bi-monthly bulletin to keep the department/agency management teams of state government better informed. We hope to consolidate most of the service update messages we send throughout the month and keep you updated about the work of the Customer Councils. If yours is one of the many departments who participated in the second annual DAS customer satisfaction survey recently, we thank you for taking the time to give us this important feedback. We look forward to sharing survey results with you, and pledge to consider responses carefully as we work to determine benchmarks and set future priorities.

Informe de les Primeres Jornades Ensenyament-Arxius "Didàctica amb fonts d'arxiu" (Barcelona, 5-7 de setembre de 2002)

El passat mes de setembre se celebraren a Barcelona les Primeres Jornades Ensenyament-Arxius que, amb el títol "Didàctica amb fonts d'arxiu", naixien de l'interès d'un grup de professionals del món dels arxius i de l'educació per fomentar la comunicació i la reflexió entre arxivers i docents, conscients de les potencialitats didàctiques de l'arxiu i els seus fons.

Iowa Workforce Monthly, August 2009, Issue 7

Highlights: *Governor Chet Culver announced a major public-private partnership between Microsoft and the State of Iowa.....pg. 2 * Daniel Skella worked with IWD after being release from prison...pg. 2 * Iowa Workforce Development works with offenders to find gainful employment after being released...pg. 3 * The Davenport Workforce Development Center has been nominated in recognition for providing older workers with job opportunities in a senior-friendly workplace...pg. 3

[Lettre autographe signée de Giuseppe Verdi à Escudier, Milan, (7 février 1869)]

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[Lettre autographe signée de Giuseppe Verdi adressée à Escudier, Sant'Agata, 7 juillet 1869]

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[Lettre autographe signée de Giuseppe Verdi adressée à Escudier, Sant'Agata, 7 août 1872]

Comprend aussi une enveloppe

Secure and Prepared Newsletter, April 8, 2010, Vol. 6, no. 7

A bi-weekly newsletter for those involved in the fields of homeland security and/or emergency management

[Lettre autographe signée de Giuseppe Verdi à Escudier, Gênes, 7 mars 1874]

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A-7 Statistical Report On FIP Applications And Cases Discontinued, July 2005

Statistical Report On FIP Applications And Cases Discontinued, July 2005

A-7 Statistical Report On FIP Applications And Cases Discontinued, August 2005

Statistical Report on FIP Applicatins and Cases Discontinued August 2005