Polymerase chain reaction based C4AQ0 and C4BQ0 genotyping: association with systemic lupus erythematosus in southwest Han Chinese

Objective: To investigate the association of complement C4 null genes (C4QO, including C4AQO and C4BQO) and C2 gene with systemic lupus erythematosus (SLE) in southwest Han Chinese; 136 patients with SLE and 174 matched controls were genotyped. Methods: C4 null genes were determined by a polymerase chain reaction (PCR) procedure with sequence specific primers (PCR-SSP). The 2 bp insertion in exon 29, which was previously identified in non-Chinese populations and caused defective C4A genes, was directly typed by sequencing the whole exon 29 using exon specific primers. The exon 6 of complement C2 was also sequenced in both the patients and controls. Results: The frequency of homozygous C4AQO allele was 12.5% (17/136) in patients with SLE compared with 1.1% (2/174) in controls (p<0.001, odds ratio (OR)=12.286, 95% confidence interval (95% CI) 2.786 to 54.170). There was no significant difference for homozygous C4BQO allele between patients with SLE and controls (p=0.699). Patients with the C4AQO gene had an increased risk of acquiring renal disorder, serositis, and anti-dsDNA antibodies compared with those without C4AQO (for renal disorder, p=0.018, OR=8.951, 95% Cl 1.132 to 70.804; for serositis, p=0.011, OR 4.891, 95% CI 1.574 to 15.198; for anti-dsDNA, p=0.004, OR 7.630, 95%Cl 1.636 to 35.584). None of the patients or controls had the 2 bp insertion in exon 29 of the C4 gene. The type I C2 deficiency was not detected in the 3 10 samples. Conclusion: It is suggested that deficiency of C4A (not due to a 2 bp insertion in exon 29), but not C4B or C2, may be a risk factor for acquiring SLE in south west Han Chinese; this results in increased risk of renal disorder, serositis, and anti-dsDNA antibodies in patients with SLE. Racial differences seem to be relevant in susceptibility to SLE.

Morphometric measurements as an index for estimating yield of meat of shell fishes, 1. Crab (Scylla serrata) Forskal

Sixty one observations on length-breadth and whole weight-meat weight relations of India crab (Scylla serrata) were made. From the length of crab (cm) the whole weight (gm) can be computed by the equation: log W=-0.1708+2.3341 log L. Similarly for any given length (cm) the meat weight (gm) can be found by the relation, log w=-1.5745+3.0148 log L.

一种改进的动物线粒体DNA提取方法

线粒体 DNA (mtDNA)已被广泛用于动物群体遗传学和进化生物学的研究, 并取得了许多有意义的结果. 有效的 mtDNA 提取方法无疑是开展这方面研究的前提. 关于动物 mtDNA 的提取方法, 国内外已有不少报导. 概括起来, 可分为: 1)氯化铯超速离心法, 2)柱层析法, 3)DNase法, 4)碱变性法. 该文报道了一种改进的碱变性提取法, 与其它方法相比, 具有应用范围广、简便、经济等优点。

The Anti-HIV Actions of 7-and 10-Substituted Camptothecins

Camptothecin (CPT), a traditional anti-tumor drug, has been shown to possess anti-HIV-1 activity. To increase the antiviral potency, the anti-HIV activities of two CPT derivatives, 10-hydroxy-CPT and 7-hydroxymethyl-CPT, were evaluated in vitro. The therapy index (TI) of CPT, 10-hydroxy-CPT and 7-hydroxymethyl-CPT against HIV-1(IIIB) in C8166 were 24.2, 4.2 and 198.1, and against clinical isolated strain HIV-1(KM018) in PBMC were 10.3, 3.5 and 66.0, respectively. While the TI of CPT, 10-hydroxy-CPT and 7-hydroxymethyl-CPT against HIV-2(CBL-20) were 34.5, 10.7 and 317.0, respectively, and the TI of the three compounds against HIV-2(ROD) showed the similar values. However, when the antiviral mechanisms were considered, we found there was no inhibition of 7-hydroxymethyl-CPT on viral cell-to-cell transmission, and was no inhibition on reverse transcriptase, protease or integrase in cell-free systems. 7-Hydroxymethyl-CPT showed no selective killing of chronically infected cells after 3 days of incubation. In conclusion, 7-hydroxymethyl-CPT showed more potent anti-HIV activity, while 10-hydroxy-CPT had less efficient activity, compared with the parent CPT. Though the antiviral mechanisms remain to be further elucidated; the modification of -OH residues at C-7 of CPT could enhance the antiviral activity, while of -OH residues at C-10 of CPT had decreased the antiviral activity, which provides the preliminary modification strategy for anti-viral activities enhancement of this compound.

中国西南部明纹花鼠三个亚种的分化

明纹花鼠是东南亚地区的特有种, 在中国有3 亚种(阿萨姆亚种、滇西亚种、滇南亚种) , 分布于 藏东南、云南西南部和南部。以往主要根据外部形态特征进行分类。为探讨3 亚种头骨性状的差异, 在测量15 个性状的基础上, 运用统计分析软件SPSS 1110 对其可测量性状进行多变量分析。结果及推测如下: ①明纹花 鼠3 个亚种的地位是确定的。②在头骨15 个性状中, 两两亚种之间具有显著差异的性状: 阿萨姆亚种与滇西亚 种有8 个(枕鼻长、吻宽、颧宽、眶间宽、后头宽、鼻骨宽、下齿列、下颌骨高) ; 阿萨姆亚种与滇西亚种有7 个(颅全长、枕鼻长、颧宽、眶间宽、后头宽、下颌高、下颌骨高) ; 滇西亚种与滇南亚种有4 个(吻宽、后头 宽、鼻骨宽、下颌高) 。③滇南亚种与滇西亚种之间的亲缘关系近于两者各自与阿萨姆亚种之间的亲缘关系。

广西云南鳅属鱼类一新种——靖西云南鳅

2008年5月,在广西壮族自治区靖两县禄洞村珠江水系采集到一批条鳅标本.经鉴定,为云南鳅属(Yunnanilus)-新种.新种靖西云南鳅(Yunnanilus jinxiensis)属于侧纹云南鳅种组(Yunnanilus pleurotaenis group).但新种全身除胸腹部外被有鳞片;侧线较长,具有15-20个侧线管孔;背鳍分枝鳍条8;胸鳍分枝鳍条13-14;鳔两室,后室发达;尾鳍凹形;体长为体高的3.9-_4.6倍,为头长的4.1-4.4倍,为尾柄高的7.5-9.0倍;头长为眼径的5.5-6.7倍,为眼间距的2.2-2.4倍等特征,可将新种与该种组内的其他种类相区分.

一篇探索真核细胞核起源的力作——读李靖炎先生的长篇近作“The primitive nucleus model and the origin of the cell nucleus”

《胞内共生与细胞研究》( 《Endocytobiosis and Cell Research》)是由国际胞内共生学会(International Society of Endocytobiology, ISE)主办的期刊, 它主要发表有关内共生物(endosy mbioses)和真核细胞的起源、发展、分化、进化和系统发育的研究论文. 在胞内共生和真核细胞的起源进化研究领域享有声誉. 去年该杂志发表了我国学者李靖炎先生的长篇论著“The primitive nucleus model and the origin of the cell nucleus(原始细胞核的模型与细胞核的起源)”(见1999, 13(1-3):1-86). 国外一家 SCI 收录的专业性刊物为中国学者发表一篇长达86页的 Review, 实不多见, 是我国学者在此领域的殊荣。

Population dynamic and stock assessment of lobster resources of Cha-bahar region (with particular ref. to Panulirus homarus)

Amoungst the three spiny lobster species in southern Iranian waters, Panulirus homarus is the only commercial species with a total landings of 20-45 tons per year indicating a decrease in both landings and CPIJE in recent years. Fishing has been regulated according to the no. of fisherman and effort, and trap ha's replaced the gillnef since 1994. Fishing is carried out along the rocky shores of Chah-Bahar region through different landing places by local fisherman for a period of about two months. Most of catch is exported mainly frozen. This research was sposored by the Fisheries Research Dept. and aimed to work on the population dynamics and stock assessment in order to stablish a better understandings of the stock and hence a proper management in this region. Sampling was done for. 10 successive months in _5 major landing places from 1994 to 1995 with no sampling during the monsoon period through June to July. Althogethere, 8500 specimen were collected and the biometry was done accordings to the sex, region and month. Average total length, total weight and carapace length was obtained 216mm., 452 gr. and 75mm. respectively. Total length-weight relationship of both sexes was calculated and follows the cubic law. Regression coefficients for both sexes was 2.8231, males 2.9616, total females 2.7490, berried females 2.6611- and non-berried females