Aplicação do teste SSW em indivíduos com perda auditiva neurossensorial usuários e não usuários de aparelho de amplificação sonora individual

OBJETIVO: comparar o desempenho de pacientes usuários e não usuários de AASI, por meio do teste SSW. MÉTODO: o estudo foi realizado em 13 sujeitos com idade entre 55 e 85 anos, com perda auditiva bilateral, sendo seis usuários de prótese auditiva bilateral e sete não usuários de prótese auditiva. O teste de processamento auditivo aplicado foi o teste de reconhecimento de dissílabos em tarefa dicótica SSW. Foi realizado um tratamento estatístico feito por meio da técnica Bootstrap e do Teste de Hipótese Kolmogorov-Smirnov. RESULTADOS: o grupo de usuários apresentou melhor desempenho nas condições estudadas do que o grupo de não usuários, principalmente nas condições competitivas. CONCLUSÃO: os resultados obtidos nessa pesquisa apontam para a eficácia do uso do AASI na melhora da compreensão de fala da população estudada, não somente pela compensação da perda auditiva periférica, mas também pela interferência no processo de envelhecimento do sistema nervoso auditivo central.

Estudo Comparativo de Três Diferentes Modalidades de Elisa para Medir os Anticorpos Contra o Vírus da Bronquite Infecciosa em Frangos Vacinados e Não Vacinados

No presente estudo, foram vacinados 150 frangos de corte de um dia de idade com sorotipo H120, e, após 28 dias desafiados à vacinação com o sorotipo M 41 do vírus da bronquite infecciosa das aves. da mesma forma, foram obtidos 150 soros de aves não vacinadas para a análise. Os respectivos soros foram analisados 28, 34 e 46 dias após o desafio, examinados através das técnicas de ELISA indireto (ELISA-I), Sandwich ELISA (S-ELISA) e ELISA com bloqueio de fase líquida (ELISA-BFL) e comparados com a técnica padrão de soroneutralização (SN) para efeito de cálculo da especificidade e sensibilidade relativas, bem como os valores predictivos positivos e negativos. O cálculo do coeficiente de correlação também foi empregado para a análise de concordância. Assim, os valores da correlação encontrados foram r = 0.98 entre ELISA-BFL x SNT, r = 0.79 entre S-ELISA x SNT e r = 0.74 ELISA-I x SNT. No entanto, quando comparamos as técnicas de ELISA entre si, ELISA-BFL x S-ELISA, ELISA-BFL x ELISA-I e S-ELISA x ELISA-I os valores encontrados foram r = 0.75, r = 0.69 e r = 0.79. A técnica de ELISA-BFL demonstrou melhor sensibilidade relativa que as técnicas de S-ELISA e ELISA-I, mesmo 46 dias após o desafio com a estirpe heteróloga. Entretanto, apesar das técnicas de S-ELISA e ELISA-I apresentarem especificidade realtiva superiores, a melhor correlação observada foi entre as técnicas de ELISA-BFL e a SN.

An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against Toxocara vitulorum in water buffaloes

Toxocara vitulorum, a parasite of the small intestine of cattle and water buffaloes, is mainly acquired by calves via the colostrum/milk from infected cows. To understand the development of immune responses in calves, antibody levels to a soluble extract antigen (Ex) from T. vitulorum infective larvae were measured by an indirect ELISA with sera of 15 buffalo calves, which were sampled every 15 days for the first 180 days after birth and 9 buffalo cows during the perinatal period. From all serum samples examined during the first 180 days, antibody level was lowest and highest in calves at 1 day of age before and after suckling colostrum, respectively, suggesting that the origin of antibodies was the colostrum. Immediately after birth, antibody levels in suckled calves remained at high levels until day 15, began to decrease to lower levels between 15 and 30 days and remained relatively stable until 120 days. By comparing the immune responses of these animals with their parasitological status it was considered possible to determine if passively acquired or actively produced antibodies provided protection against the infection. High numbers of T. vitulorum eggs in the feces between 30 and 60 days indicated that passively acquired antibodies did not provide protection against the infection, at least during these first days, and the maximum fecal egg counts during 30-45 days were coincident with decreased antibody levels. Between 60 and 120 days, when serum antibodies were detected at reduced, but stable levels, adult nematodes were expelled from the intestines and no more T. vitulorum eggs were found, suggesting development of acquired resistance. However, the potential and functional protective role of the antibodies against T. vitulorum infection and the process of self-cure requires further investigation. (C) 2001 Elsevier B.V. B.V. All rights reserved.

EXAMINATION BY ELISA OF SERA OBTAINED FROM CHICKEN BREEDER AND LAYER FLOCKS SHOWING EVIDENCE OF FOWL TYPHOID OR PULLORUM DISEASE

An indirect ELISA using soluble whole cell antigen was used to screen serum samples obtained from breeder and layer flocks some of which had shown clinical or bacteriological evidence of infection with Salmonella Gallinarum or S. Pullorum. There was good correlation between Salmonella infection and the presence of serum samples showing high optical density (OD) values. Sera from seven flocks showing high values were retested using group D (0-1, 9, 12) lipopolysaccharide (LPS) and g,m-H flagella as detecting antigens. Sera from six flocks produced high OD values with LPS and low values with flagella confirming infection with a non-flagellate, group D Salmonella while one produced high values with both antigens indicating mixed infection with another group D serotype.

Immunodiagnosis of swine cysticercosis by indirect ELISA employing a heterologous antigen from Taenia crassiceps metacestode

C. Larralde et al. (1990, Arch. Pathol. Lab. Med., 114:926-928) demonstrated that heterologous antigen from the laboratory-adapted murine Taenia crassiceps metacestode may substitute those from Taenia solium in the immunodiagnosis of human cysticercosis by the indirect enzyme-linked immunosorbent assay (IE). ?This antigen is easily obtained at a laboratory level and solves the problem of T. solium cysticerci collection from naturally or experimentally infected swine. In this study an IE employing a heterologous antigen from the T. crassiceps metacestode was evaluated for the immunodiagnosis of swine cysticercosis. Sera from 300 swine free of T. solium cysticerci by post-mortem examination were employed to determine two IE cut- off values: 1) Mean ELISA values + 2 standard deviations (2 sigma cut-off) and 2) - Mean ELISA values + 3 standard deviations (3 sigma cut-off). The specificity of IE was 97% with the 2 sigma cut-off and 100% with the 3 sigma cut-off. When applied to ten sera from swine infected by cysticerci of T. solium by post-mortem examination, the sensitivity of IE was 100% independent of the cut off.

A validação do Teste de Juízo Moral (MJT) para diferentes culturas: o caso brasileiro

The present article deals with the adaptation of the Moral Judgment Test (MJT) for the Portuguese language. The principles of the test, its format and the process of content, construct and criterion validation are presented. In the same way, reliability is discussed using Brazilian research results that produced the necessity of elaborating another dilemma to compose the MJT-xt (extended).

Teste de envelhecimento acelerado em sementes de quiabo

The objective of this research was to study the accelerated aging test to evaluate the okra vigor seed. Were used four okra seed lots cv. "Santa Cruz". The initial seed lot quality was evaluate by the moisture content, germination, first germination count, speed of germination index and seedling emergence. The experiment was carried out in two phases: the first one the seeds were submitted to the accelerate aging with and without NaCl salt solution aging periods of 48, 72, 96 and 120 hours, at 41 and 45(0) C temperature. The second phase the best procedures as for as accelerated aging and temperatures were repeated. The period of 72h and 41 C with and without NaCl saturated solution was the most adequate for the lot classification in distint vigor levels.

Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Validation of a Leishmania infantum ELISA rapid test for serological diagnosis of Leishmania chagasi in dogs

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Vermiculita como substrato para o teste de germinação de sementes de barbatimão

The objective of this research was to evaluate the use of vermiculite as a substrate for the germination test of 'barbatimao' seeds and to establish what would be the best granulometry and the best moisture intensity to germinate 'barbatimao' seeds. Four replications of 50 seeds were previously scarified with H(2)SO(4) for 60 minutes. Afterwards, the following germination substrates were evaluated: paper rolls moistened with water equivalent to 2.5 times the weight of the dry paper (control); micron, super thin, thin and average sized vermiculite moistened with 0.5, 1.0, 1.5 and 2.0 times the weight of the dry paper. The germination temperature was 30 C. The percentage of normal seedlings was obtained seven days after sowing (first count) and, then, weekly (up to 28 days). Average time and relative distribution of germination were calculated. It was concluded that the germination of 'barbatimao' seeds can be carried out with either fine vermiculite moistened with water equivalent to 2.0 times the weight of the dry paper or average sized substrate moistened with water equivalent to 0.5 to 2.0 times the weight of dry paper. These treatments resulted in higher and faster germination (21 days), similarly to the paper roll method.

Aplicacao do teste de qui-quadrado na verificacao da variacao de leituras densitometricas

The densitometrical interpretation is a technical resource that removes both the subjectivity of conventional interpretation and the high operational cost of automatic interpretation. Meanwhile, the densitometric lecture presents variability and this work has the objective of examinating, through Chi-square test, if there exists significative differences of cone overture of the densitometer as to the filter used. The results were the following: the variation that happened in this densitometric lecture, when different cone overture and filter were used, have not invalidated the values that were obtained; the extreme values variations have to be used with attention, so will not be overstep of values among the different elements of soil covering have to be used discerniment to classify this values; the study was in consonance with other authors. -from English summary