Data base on pelagic ciliates grazing and growth rate as a function of size, prey size and type compiled from the literature

Microzooplankton (the 20 to 200 µm size class of zooplankton) is recognised as an important part of marine pelagic ecosystems. In terms of biomass and abundance pelagic ciliates are one of the important groups of organism in microzooplankton. However, their rates - grazing and growth - , feeding behaviour and prey preferences are poorly known and understood. A set of data was assembled in order to derive a better understanding of pelagic ciliates rates, in response to parameters such as prey concentration, prey type (size and species), temperature and their own size. With these objectives, literature was searched for laboratory experiments with information on one or more of these parameters effect studied. The criteria for selection and inclusion in the database included: (i) controlled laboratory experiment with a known ciliates feeding on a known prey; (ii) presence of ancillary information about experimental conditions, used organisms - cell volume, cell dimensions, and carbon content. Rates and ancillary information were measured in units that meet the experimenter need, creating a need to harmonize the data units after collection. In addition different units can link to different mechanisms (carbon to nutritive quality of the prey, volume to size limits). As a result, grazing rates are thus available as pg C/(ciliate*h), µm**3/(ciliate*h) and prey cell/(ciliate*h); clearance rate was calculated if not given and growth rate is expressed as the growth rate per day.

Producción de biogás a partir de biomasa de la microalga scenedesmus sp. procedente de diferentes procesos

En este trabajo de Tesis Doctoral se ha estudiado la posibilidad de emplear las microalgas, concretamente el género Scenedesmus, como sustrato para la producción de biogás mediante digestión anaerobia, así como los residuos que se producen como consecuencia de su utilización industrial para diferentes fines. La utilización de las microalgas para la producción de biocombustibles es un tema de gran actualidad científica, en el que residen muchas expectativas para la producción a gran escala de biocombustibles que supongan una alternativa real a los combustibles fósiles. Existen numerosas investigaciones sobre la conversión a biogás de las microalgas, sin embargo aún hay poco conocimiento sobre la utilización de la digestión anaerobia como tratamiento de residuos de microalgas en un concepto de biorrefinería. Residuos que pueden ser generados tras la extracción de compuestos de alto valor añadido (p. ej. aminoácidos) o tras la generación de otro biocombustible (p. ej. biodiésel). Es en este aspecto en el que esta Tesis Doctoral destaca en cuanto a originalidad e innovación, ya que se ha centrado principalmente en tres posibilidades: - Empleo de Scenedesmus sp. como cultivo energético para la producción de biogás. - Tratamiento de residuos de Scenedesmus sp. generados tras la extracción de aminoácidos en un concepto de biorrefinería. - Tratamiento de los residuos de Scenedesmus sp. generados tras la extracción de lípidos en un concepto de biorrefinería. Los resultados obtenidos demuestran que la microalga Scenedesmus como cultivo energético para producción de biogás no es viable salvo que se empleen pretratamientos que aumenten la biodegradabilidad o se realice codigestión con otro sustrato. En este último caso, la chumbera (Opuntia maxima Mill.) ha resultado ser un sustrato idóneo para la codigestión con microalgas, aumentando la producción de biogás y metano hasta niveles superiores a 600 y 300 L kgSV-1, respectivamente. Por otro lado, el tratamiento de residuos generados tras la extracción de aminoácidos mediante digestión anaerobia es prometedor. Se obtuvieron elevados rendimientos de biogás y metano en las condiciones de operación óptimas (409 y 292 L kgSV-1, respectivamente). Aparte de la generación energética por medio el metano, que podría emplearse en la propia biorrefinería o venderse a la red eléctrica o de gas natural, reciclando el digerido y el CO2 del biogás se podría llegar a ahorrar alrededor del 30% del fertilizante mineral y el 25% del CO2 necesarios para el cultivo de nueva biomasa. Por lo tanto, la digestión anaerobia de los residuos de microalgas en un concepto de biorrefinería tiene un gran potencial y podría contribuir en gran medida al desarrollo de esta industria. Por último, una primera aproximación al tratamiento de residuos generados tras la extracción de lípidos muestra que éstos pueden ser empleados para la producción de biogás, como monosustrato, o en codigestión con glicerina, ya que son fácilmente biodegradables y el rendimiento potencial de metano puede alcanzar 218 LCH4 kgSV-1 y 262 LCH4 kg SV-1 en monodigestión o en codigestión con glicerina, respectivamente. ABSTRACT This PhD thesis explores the possibility of using microalgae, specifically the strain Scenedesmus, as substrate for biogas production through anaerobic digestion, as well as the residues generated after its use in different industrial processes. The use of microalgae for biofuels production is an emerging scientific issue. The possibility of producing biofuels from microalgae as a real alternative for fossil fuels is raising high expectations. There are several research projects on the conversion of microalgae to biogas; however, there is little knowledge about using anaerobic digestion for treating microalgae residues in a biorefinery scheme. These residues could be generated after the extraction of high value compounds (e.g. amino acids) or after the production of another biofuel (e.g. biodiesel). It is in this area in which this PhD thesis stands in terms of originality and innovation, since it has focused primarily on three possibilities: - The use of Scenedesmus sp. as an energy crop for biogas production. - Treatment of amino acid extracted Scenedesmus residues generated in a biorefinery. - Treatment of lipid extracted Scenedesmus residues generated in a biorefinery. The results obtained in this work show that the use of Scenedesmus as energy crop for biogas production is not viable. The application of pretreatments to increase biodegradability or the codigestion of Scenedesmus biomass with other substrate can improve the digestion process. In this latter case, prickly pear (Opuntia maxima Mill.) is an ideal substrate for its codigestion with microalgae, increasing biogas and methane yields up to more than 600 and 300 L kgVS-1, respectively. On the other hand, the treatment of residues generated after amino acid extraction through anaerobic digestion is promising. High biogas and methane yields were obtained (409 y 292 L kgVS-1, respectively). Besides the energy produced through methane, which could be used in the biorefinery or be sold to the power or natural gas grids, by recycling the digestate and the CO2 30% of fertilizer needs and 25% of CO2 needs could be saved to grow new microalgae biomass. Therefore, the anaerobic digestion of microalgae residues generated in biorefineries is promising and it could play an important role in the development of this industry. Finally, a first approach to the treatment of residues generated after lipid extraction showed that these residues could be used for the production of biogas, since they are highly biodegradable. The potential methane yield could reach 218 LCH4 kgVS-1 when they are monodigested, whereas the potential methane yield reached 262 LCH4 kgVS-1 when residues were codigested with residual glycerin.

HISTÓRIA E MEMÓRIA DA ESCOLA NORMAL PARTICULAR DO INSTITUTO SANTA TERESA DE LORENA/SP (1964-1974)

O presente trabalho pretende mostrar, à luz da abordagem sócio-histórica ou histórico-sociológica, a formação da professora primária na Escola Normal Particular Santa Teresa, uma instituição de irmãs salesianas que se dedicam à educação em Lorena-SP desde 1954. O problema da parte da seguinte indagação: como as irmãs salesianas do Instituto Santa Teresa em Lorena/SP formaram a professora primária, no período de 1964 -1974? Os objetivos que norteiam o problema da pesquisa são: compreender os motivos da criação e extinção da Escola Normal Particular Santa Teresa; conhecer a clientela inicial e quem a freqüentou durante seu funcionamento; descrever a constituição do corpo docente; verificar a legislação pertinente a essa escola; desvelar indícios das práticas pedagógicas. O presente estudo, compreendido entre os anos de 1964 a 1974, fundamenta-se nos aportes teóricos de Mogarro, sobre os arquivos escolares; Demartini, sobre memória e relatos orais; Saviani e outros pesquisadores que tecem novas concepções históricas de instituições escolares no Brasil. A partir dos estudos dos documentos da Escola Normal e da analise dos relatos dos sujeitos ntrevistados percebe-se que: as irmãs salesianas do Instituto Santa Teresa ao formarem a moça professora tinham como finalidade a formação da moça, da mãe, da esposa, da mulher.(AU)

HISTÓRIA E MEMÓRIA DA ESCOLA NORMAL PARTICULAR DO INSTITUTO SANTA TERESA DE LORENA/SP (1964-1974)

O presente trabalho pretende mostrar, à luz da abordagem sócio-histórica ou histórico-sociológica, a formação da professora primária na Escola Normal Particular Santa Teresa, uma instituição de irmãs salesianas que se dedicam à educação em Lorena-SP desde 1954. O problema da parte da seguinte indagação: como as irmãs salesianas do Instituto Santa Teresa em Lorena/SP formaram a professora primária, no período de 1964 -1974? Os objetivos que norteiam o problema da pesquisa são: compreender os motivos da criação e extinção da Escola Normal Particular Santa Teresa; conhecer a clientela inicial e quem a freqüentou durante seu funcionamento; descrever a constituição do corpo docente; verificar a legislação pertinente a essa escola; desvelar indícios das práticas pedagógicas. O presente estudo, compreendido entre os anos de 1964 a 1974, fundamenta-se nos aportes teóricos de Mogarro, sobre os arquivos escolares; Demartini, sobre memória e relatos orais; Saviani e outros pesquisadores que tecem novas concepções históricas de instituições escolares no Brasil. A partir dos estudos dos documentos da Escola Normal e da analise dos relatos dos sujeitos ntrevistados percebe-se que: as irmãs salesianas do Instituto Santa Teresa ao formarem a moça professora tinham como finalidade a formação da moça, da mãe, da esposa, da mulher.(AU)

A (R)EXISTÊNCIA DAS VOCACIONADAS AO MINISTÉRIO PASTORAL BATISTA: DESCORTINANDO A RELAÇÃO ENTRE AS PASTORAS BATISTAS DE SÃO PAULO E A NÃO FILIAÇÃO NA ORDEM DOS PASTORES BATISTAS DO BRASIL EM SÃO PAULO (OPBB-SP)

O contexto batista é predominantemente marcado por lideranças masculinas, destinando às mulheres apenas lugares e comportamentos socialmente estabelecidos, como a casa, o cuidado, a maternidade, a submissão, entre outras características que enfatizam a hierarquia de gênero. Mesmo diante do desenvolvimento econômico e da ocupação que as mulheres estão conquistando no campo público, a igreja e principalmente as igrejas batistas, permanecem fundadas em alicerces que exaltam o poder masculino em detrimento do lugar que deve ser ocupado pelas mulheres, ou seja, onde elas decidirem atuar. Caso elas decidam atuar num campo predominantemente masculino, terão que lidar com a desconstrução de um pensamento socialmente permeado de dominação masculina e com a árdua construção de um pensamento que vise a igualdade de gênero. O objeto desta pesquisa é o ministério pastoral feminino no contexto batista brasileiro. O texto analisa o discurso das Pastoras Batistas do Estado de São Paulo e o discurso dos líderes da Ordem dos Pastores Batistas de São Paulo (OPBB-SP) a respeito do ministério pastoral feminino e a não filiação de mulheres na OPBB-SP. A importância deste trabalho é a de demostrar as relações de micro poder existentes entre pastores e pastoras e concomitantemente as desigualdades dentro do contexto batista com relação ao ministério pastoral feminino. Essa afirmação se consolida por meio das análises das entrevistas semiestruturadas que realizei na pesquisa de campo, com sete pastoras batistas do Estado de São Paulo, bem como com três líderes da OPPB-SP. Esta é uma pesquisa qualitativa, em que foram analisados documentos oficiais da igreja, como pautas de convenções, atas, sites institucionais, periódicos e documentos não oficiais encontrados em redes sociais, blogs, jornais online, entre outros. Posso afirmar que as pastoras batistas estão se mobilizando para cumprir sua vocação, usando argumentos transcendentes que impedem qualquer pessoa de desafiar ou duvidar de seu chamado pastoral, pois: “O vento sopra onde quer; ouve-se o ruído, mas não sabes de onde vem, nem para onde vai. Assim acontece com aquele(a) que nasceu do Esp��rito.” (João 3.8).

Na contramão do marketing político: as eleições municipais de Taubaté-SP, em 2004

Este estudo trata da investigação das estratégias de propaganda política utilizadas nos programas de TV de Roberto Peixoto e de Antônio Mário Mattos Ortiz, que concorreram às eleições para prefeito do município de Taubaté-SP, no ano de 2004. O objetivo central da pesquisa é entender a interferência dos modelos globais da propaganda política de TV no contexto local, em uma época em que se tornou comum a contratação de agências de publicidade de renome para a realização de campanhas eleitorais em cidades do interior. Utilizou-se, como metodologia, pesquisas histórica, documental e bibliográfica, além de análise de conteúdo dos programas de TV de ambos os candidatos e entrevistas semi-estruturadas com políticos e profissionais da área de marketing político. Dentre as principais contribuições, verificou-se que a vitória nas urnas no contexto local deve-se mais ao carisma de determinados líderes políticos, bem como aos aspectos históricos, culturais e sociológicos da localidade em questão do que à mágica anunciada pelo mercado publicitário quanto às estratégias globais da propaganda política na televisão.

Electrical stimulation of neonatal cardiomyocytes results in the sequential activation of nuclear genes governing mitochondrial proliferation and differentiation

Electrical stimulation of neonatal cardiac myocytes produces hypertrophy and cellular maturation with increased mitochondrial content and activity. To investigate the patterns of gene expression associated with these processes, cardiac myocytes were stimulated for varying times up to 72 hr in serum-free culture. The mRNA contents for genes associated with transcriptional activation [c-fos, c-jun, JunB, nuclear respiratory factor 1 (NRF-1)], mitochondrial proliferation [cytochrome c (Cyt c), cytochrome oxidase], and mitochondrial differentiation [carnitine palmitoyltransferase I (CPT-I) isoforms] were measured. The results establish a temporal pattern of mRNA induction beginning with c-fos (0.25–3 hr) and followed sequentially by c-jun (0.5–3 hr), JunB (0.5–6 hr), NRF-1 (1–12 hr), Cyt c (12–72 hr), and muscle-specific CPT-I (48–72 hr). Induction of the latter was accompanied by a marked decrease in the liver-specific CPT-I mRNA, thus supporting the developmental fidelity of this pattern of gene regulation. Consistent with a transcriptional mechanism, electrical stimulation increased c-fos, β-myosin heavy chain, and Cyt c promoter activities. These increases coincided with a rise in their respective endogenous gene transcripts. NRF-1, cAMP response element, and Sp-1 site mutations within the Cyt c promoter reduced luciferase expression in both stimulated and nonstimulated myocytes. Mutations in the NRF-1 and CRE sites inhibited the induction by electrical stimulation (5-fold and 2-fold, respectively) whereas mutation of the Sp-1 site maintained or increased the fold induction. This finding is consistent with the appearance of NRF-1 and fos/jun mRNAs prior to that of Cyt c and suggests that induction of these transcription factors is a prerequisite for the transcriptional activation of Cyt c expression. These results support a regulatory role for NRF-1 and possibly AP-1 in the initiation of mitochondrial proliferation.

Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

The gene encoding 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO; EC 1.14.12.4) was cloned by using an oligonucleotide probe corresponding to the N terminus of the enzyme to screen a DNA library of Pseudomonas sp. MA-1. The gene encodes for a protein of 379 amino acid residues corresponding to a molecular mass of 41.7 kDa, the same as that previously estimated for MHPCO. MHPCO was expressed in Escherichia coli and found to have the same properties as the native enzyme from Pseudomonas sp. MA-1. This study shows that MHPCO is a homotetrameric protein with one flavin adenine dinucleotide bound per subunit. Sequence comparison of the enzyme with other hydroxylases reveals regions that are conserved among aromatic flavoprotein hydroxylases.

Desensitization of the Neurokinin-1 Receptor (NK1-R) in Neurons: Effects of Substance P on the Distribution of NK1-R, Gαq/11, G-Protein Receptor Kinase-2/3, and β-Arrestin-1/2

Observations in reconstituted systems and transfected cells indicate that G-protein receptor kinases (GRKs) and β-arrestins mediate desensitization and endocytosis of G-protein–coupled receptors. Little is known about receptor regulation in neurons. Therefore, we examined the effects of the neurotransmitter substance P (SP) on desensitization of the neurokinin-1 receptor (NK1-R) and on the subcellular distribution of NK1-R, Gαq/11, GRK-2 and -3, and β-arrestin-1 and -2 in cultured myenteric neurons. NK1-R was coexpressed with immunoreactive Gαq/11, GRK-2 and -3, and β-arrestin-1 and -2 in a subpopulation of neurons. SP caused 1) rapid NK1-R–mediated increase in [Ca2+]i, which was transient and desensitized to repeated stimulation; 2) internalization of the NK1-R into early endosomes containing SP; and 3) rapid and transient redistribution of β-arrestin-1 and -2 from the cytosol to the plasma membrane, followed by a striking redistribution of β-arrestin-1 and -2 to endosomes containing the NK1-R and SP. In SP-treated neurons Gαq/11 remained at the plasma membrane, and GRK-2 and -3 remained in centrally located and superficial vesicles. Thus, SP induces desensitization and endocytosis of the NK1-R in neurons that may be mediated by GRK-2 and -3 and β-arrestin-1 and -2. This regulation will determine whether NK1-R–expressing neurons participate in functionally important reflexes.

Stepwise Photoinhibition of Photosystem II1 : Studies with Synechocystis Species PCC 6803 Mutants with a Modified D-E Loop of the Reaction Center Polypeptide D1

Several mutant strains of Synechocystis sp. PCC 6803 with large deletions in the D-E loop of the photosystem II (PSII) reaction center polypeptide D1 were subjected to high light to investigate the role of this hydrophilic loop in the photoinhibition cascade of PSII. The tolerance of PSII to photoinhibition in the autotrophic mutant ΔR225-F239 (PD), when oxygen evolution was monitored with 2,6-dichloro-p-benzoquinone and the equal susceptibility compared with control when monitored with bicarbonate, suggested an inactivation of the QB-binding niche as the first event in the photoinhibition cascade in vivo. This step in PD was largely reversible at low light without the need for protein synthesis. Only the next event, inactivation of QA reduction, was irreversible and gave a signal for D1 polypeptide degradation. The heterotrophic deletion mutants ΔG240-V249 and ΔR225-V249 had severely modified QB pockets, yet exhibited high rates of 2,6-dichloro-p-benzoquinone-mediated oxygen evolution and less tolerance to photoinhibition than PD. Moreover, the protein-synthesis-dependent recovery of PSII from photoinhibition was impaired in the ΔG240-V249 and ΔR225-V249 mutants because of the effects of the mutations on the expression of the psbA-2 gene. No specific sequences in the D-E loop were found to be essential for high rates of D1 polypeptide degradation.

The peptide binding site of the substance P (NK-1) receptor localized by a photoreactive analogue of substance P: presence of a disulfide bond.

Substance P (SP) is a neuropeptide that mediates multiple physiological responses including transmission of painful stimuli and inflammation via an interaction with a receptor of known primary sequence. To identify the regions of the SP receptor, also termed the NK-1 receptor, involved in peptide recognition, we are using analogues of SP containing the photoreactive amino acid p-benzoyl-L-phenylalanine (Bpa). In the present study, we used radioiodinated Bpa8-SP to covalently label with high efficiency the rat SP receptor expressed in a transfected mammalian cell line. To identify the amino acid residue that serves as the site of covalent attachment, a membrane preparation of labeled receptor was subjected to partial enzymatic cleavage by trypsin. A major digestion product of 22 kDa was identified. Upon reduction with 2-mercaptoethanol the mass of this product decreased to 14 kDa. The 22-kDa tryptic fragment was purified in excellent yield by preparative SDS/PAGE under nonreducing conditions. Subcleavage with Staphylococcus aureus V8 protease and endoproteinase ArgC yielded fragments of 8.2 and 9.0 kDa, respectively. Upon reductive cleavage, the V8 protease fragment decreased to 3.0 kDa while the endoproteinase ArgC fragment decreased to 3.2 kDa. Taking into consideration enzyme specificity, molecular size, determination of the presence or absence of N-glycosylation sites, and recognition by antibodies to specific sequences of the SP receptor, the V8 protease fragment is Thr-173 to Glu-183, while the endoproteinase ArgC fragment is Val-178 to Arg-190. These two fragments share the common sequence Val-Val-Cys-Met-Ile-Glu (residues 178-183). The site of covalent attachment of radioiodinated Bpa8-SP is thus restricted to a residue within this overlap sequence. The data presented here also establish that the cysteine residue in this sequence Cys-180, which is positioned in the middle of the second extracellular loop, participates in a disulfide bond that links the first and second extracellular loops of the receptor.