06025-012 Rathbun Lake Watershed Final Report

The Rathbun Land and Water Alliance and partners have implemented a unique approach to water quality protection through the Rathbun Lake Special Project. This approach is achieving a significant reduction in the sediment and phosphorus that impair water quality in Rathbun Lake and its tributaries as a result of the targeted application of best management practices (BMPs) for priority land in the watershed. This project application proposes to assist landowners to apply BMPs that will reduce sediment and phosphorus delivery from priority land in two targeted sub-watersheds as part of the Rathbun Lake Special Project Features of this project are: (1) use of geographic information system (GIS) analysis to identify priority land that requires BMPs; (2) assistance for landowners to apply BMPs on more than 2,200 acres that will reduce sediment and phosphorus delivery by 3,300 tons and 13,300 pounds per year respectively; (3) evaluation of the benefits from BMP application using GIS analysis and water quality monitoring; and (4) watershed outreach activities that encourage landowners to apply BMPs for priority land to protect water quality.

05005-002 Farmers Creek Watershed Final Report

Farmers Creek is a moderately flowing stream that winds through seventeen miles of central Jackson County, encompassing a watershed area of 30,590 acres. Due to nutrient loading and sedimentation, the stream was placed on Iowa’s 303(d) List of Impaired Waters in 2002. A three year grant project was initiated in January 2005 to reduce both sediment delivery and phosphorus levels by 40% in critical areas along the stream corridor. Over thirty-five BMP’s were started in the first nine months of this project. Funding through WIRB is being requested specifically for streambank stabilization and protection projects not covered by other cost-share programs. Innovative project designs and techniques will be installed and act as demonstration sites. Projects may include jetties, weirs, cedar revetments, cattle crossings, and fencing. To assist in excluding cattle from the stream, watering systems such as slingpumps, pasture pumps, or water rams will be installed, in conjunction with filter strips and riparian buffers.

Combining phospholipases and a liquid lipase for one-step biodiesel production using crude oils

Background Enzymatic biodiesel is becoming an increasingly popular topic in bioenergy literature because of its potential to overcome the problems posed by chemical processes. However, the high cost of the enzymatic process still remains the main drawback for its industrial application, mostly because of the high price of refined oils. Unfortunately, low cost substrates, such as crude soybean oil, often release a product that hardly accomplishes the final required biodiesel specifications and need an additional pretreatment for gums removal. In order to reduce costs and to make the enzymatic process more efficient, we developed an innovative system for enzymatic biodiesel production involving a combination of a lipase and two phospholipases. This allows performing the enzymatic degumming and transesterification in a single step, using crude soybean oil as feedstock, and converting part of the phospholipids into biodiesel. Since the two processes have never been studied together, an accurate analysis of the different reaction components and conditions was carried out. Results Crude soybean oil, used as low cost feedstock, is characterized by a high content of phospholipids (900 ppm of phosphorus). However, after the combined activity of different phospholipases and liquid lipase Callera Trans L, a complete transformation into fatty acid methyl esters (FAMEs >95%) and a good reduction of phosphorus (P <5 ppm) was achieved. The combination of enzymes allowed avoidance of the acid treatment required for gums removal, the consequent caustic neutralization, and the high temperature commonly used in degumming systems, making the overall process more eco-friendly and with higher yield. Once the conditions were established, the process was also tested with different vegetable oils with variable phosphorus contents. Conclusions Use of liquid lipase Callera Trans L in biodiesel production can provide numerous and sustainable benefits. Besides reducing the costs derived from enzyme immobilization, the lipase can be used in combination with other enzymes such as phospholipases for gums removal, thus allowing the use of much cheaper, non-refined oils. The possibility to perform degumming and transesterification in a single tank involves a great efficiency increase in the new era of enzymatic biodiesel production at industrial scale.

05031-011 Rathbun Lake Watershed Final Report

This project proposes a unique approach to addressing one of the more challenging issues facing efforts to protect water quality in Rathbun Lake; reducing sediment and phosphorus delivery from land on which best management practices (BMPs) will not be applied. This project will construct ten impoundments at key locations in the watershed to reduce the annual delivery of 2,970 tons of sediment and 12,100 pounds of phosphorus to Rathbun Lake. Features of this approach are: (a) impoundments will be constructed at sites below areas with high concentrations of priority land that has the greatest potential to deliver sediment and phosphorus to Rathbun Lake and on which landowners are unable or unwilling to apply BMPs and (b) strategically placed, large impoundments can be a cost effective means of reducing the sediment and phosphorus delivered to Rathbun Lake from priority land on which BMPs will not be applied. This project will significantly enhance the success of efforts underway in the Rathbun Lake Special Project as it addresses a critical water quality protection need that is not being met by current Special Project activities.

05002-001 Little Pony Creek Watershed Final Report

Farmers Creek is a moderately flowing stream that winds through seventeen miles of central Jackson County, encompassing a watershed area of 30,590 acres. Due to nutrient loading and sedimentation, the stream was placed on Iowa’s 303(d) List of Impaired Waters in 2002. A three year grant project was initiated in January 2005 to reduce both sediment delivery and phosphorus levels by 40% in critical areas along the stream corridor. Over thirty-five BMP’s were started in the first nine months of this project. Funding through WIRB is being requested specifically for streambank stabilization and protection projects not covered by other cost-share programs. Innovative project designs and techniques will be installed and act as demonstration sites. Projects may include jetties, weirs, cedar revetments, cattle crossings, and fencing. To assist in excluding cattle from the stream, watering systems such as slingpumps, pasture pumps, or water rams will be installed, in conjunction with filter strips and riparian buffers.

05035-012 Clear Lake Watershed Final Report

Clear Lake, Iowa's third largest natural lake, is located in Cerro Gordo County in north-central Iowa. The lake is a premier natural resource and popular recreational destination in north central Iowa, providing more than $40 million dollars annually to the local economy. Despite the lake's already strong recreational use, water quality concerns have not allowed the lake to reach its full recreational potential. Clear Lake is listed on Iowa's 2004 303(d) Impaired Waters List due to excessive levels of phosphorus, bacteria, and turbidity. Urban storm water runoff from the 8,600 acre watershed is a significant contributor to Clear Lake's impairment. There are over 68 storm water outlet points in the Clear Lake watershed that allow untreated urban runoff to empty directly into Clear Lake. Local governments have been very active in installing storm water Best Management Practices (BMPs) at as many of the outlets that current funding allows. To date, 11 of the 68 sites (16%) have been protected. These improvements have been very effective in reducing contaminants in the storm water runoff, but the remaining outlets still need protection. The first phase of this grant request is for an investigation of 10 storm water outlet sites to determine the most cost effective BMP for each site. The second phase of the grant request is to implement the BMPs deemed most cost effective at 5 of the 10 sites investigated. The grant request addresses one of the main priorities of the Iowa Watershed Improvement Grant: storm water runoff.

1240-017 Little Bear Creek Watershed Final Report

Little Bear Creek is a 21.79 mile Class A1 and B (WW2) warm water stream that encompasses approximately 29,202 acres in northern Poweshiek County. The lower 8.4 mile segment is listed as biologically impaired on both the IDNR 2008 303(d) list and 2010 303(d) draft list. A RASCAL assessment and landowner survey was completed through a development grant in 2011, and these assessments indicate that erosion and sediment delivery from cropland, lack of adequate buffers along the stream channel, and streambank conditions contribute significant sediment delivery to the stream, likely resulting in the impairment. An estimated 36,544 tons of sediment are delivered to the stream annually. A total of 11,075 acres (38%) of the watershed are high priority areas or land with sediment delivery rates greater than one. Our goal over 15 years is to install Best Management Practices (BMPs) and increase public education in order to reduce sediment and phosphorus delivery by 25% and decrease priority areas by 15%. More specific objectives for this WIRB project are to 1) Reduce annual sediment delivery by roughly 16.3% or 1,058 tons and associated phosphorus delivery by 1,375 pounds and 2) Develop an information and education program aimed at producers and residents within the headwaters of Grant and Chester townships, which account for 18% of the watershed's priority areas. The SWCD proposes to utilize 50% EQIP funds and 25% WIRB funds toward rural BMPs, and 75% WIRB funds toward urban BMPs received through this application.

Polyunsaturated aldehydes from large phytoplankton of the Atlantic Ocean Surface (42ºN to 33ºS)

Polyunsaturated aldehydes (PUAs) are organic compounds mainly produced by diatoms, after cell wounding. These compounds are increasingly reported as teratogenic for species of grazers and deleterious for phytoplanktonic species, but there is still scarce information regarding concentration ranges and the composition of PUAs in the open ocean. In this study, we analyzed the spatial distribution and the type of aldehydes produced by the large-sized (>10 μm) phytoplankton in the Atlantic Ocean surface. Analyses were conducted on PUAs released after mechanical disruption of the phytoplankton cells, referred to here as potential PUAs (pPUAs). Results show the ubiquitous presence of pPUA in the open ocean, including upwelling areas, as well as oligotrophic gyres. Total pPUA concentrations ranged from zero to 4.18 pmol from cells in 1 L. Identified PUAs were heptadienal, octadienal and decadienal, with heptadienal being the most common (79% of total stations). PUA amount and composition across the Atlantic Ocean was mainly related to the nitrogen:phosphorus ratio, suggesting nutrient-driven mechanisms of PUA production. Extending the range of trophic conditions considered by adding data reported for productive coastal waters, we found a pattern of PUA variation in relation to trophic status.

Urinary saturation and nephrocalcinosis in preterm infants: effect of parenteral nutrition.

Urinary lithogenic and inhibitory factors were studied in 27 preterm infants; 16 had total parenteral nutrition (TPN) and 11 had breastmilk with an additional glucose-sodium chloride infusion. Urines were collected for 24 hours on day 2 (period A), day 3 (B), and once between days 4 and 10 (C). Urinary calcium oxalate saturation was calculated by the computer program EQUIL 2. Renal ultrasonography was performed every second week until discharge. The calcium/creatinine ratio increased in infants on TPN (A 0.91; C 1.68 mol/mol) and was significantly higher at period C than that in infants on breastmilk/infusion (A 0.52; C 0.36). The oxalate/creatinine ratio was persistently higher with TPN (203 mmol/mol) than with breastmilk/infusion (98; 137). The citrate/creatinine remained constant with TPN (0.44 mol/mol), whereas it increased significantly with breastmilk/infusion (0.26; 0.49). Calcium/citrate rose considerably with TPN, but decreased with breastmilk/infusion to a significantly lower level than with TPN. The urinary calcium oxalate saturation increased with TPN (2.4; 4.5) and decreased with breastmilk/infusion (2.1; 1.5) to a significantly lower value than with TPN. Nephrocalcinosis developed in two infants on TPN. Mean daily calcium intake was similar in both groups, whereas protein, sodium, and phosphorus intake were significantly higher on TPN. It is concluded that the increase in urinary calcium oxalate saturation observed with TPN is due to the combined effect of an increased urinary calcium excretion and higher urinary oxalate/creatinine and calcium/citrate ratios. The changes observed are likely to be caused by TPN itself, which differs in several respects from breastmilk feeding.

Kalkkisaostuslaitteiston suunnittelu Biolan Oy:n lannoite- ja kasvualustatehtaan varastoja ja kompostointikentältä tulevien suoto- ja hulevesien käsittelyyn

Tässä diplomityössä oli tavoitteena löytää ratkaisu Biolan Oy:n lannoiteja kasvualustatehtaan varastoja kompostointikentältä tulevien suotoja hulevesien käsittelyyn. Erityisenä kiinnostuksen kohteena oli suotoja hulevesien sisältämän fosforin poistamisen ratkaisun löytäminen. Ratkaisua päätettiin lähteä hakemaan kemiallisen saostuksen kautta. Fosforin kemiallinen saostus toteutetaan nykypäivänä useimmiten raudan ja alumiini yhdisteiden avulla, mutta tämän työn lähtökohtana oli saostuksen suorittaminen kalkilla. Yhtenä työn lähtökohtana oli myös muodostuvan, fosforipitoisen, kalkkisakan hyödyntäminen Biolan Oy:n kasvualustatehtaan tuottamien kasvualustojen ravinnelähteenä, jolloin yleisesti ongelmana pidetty jätevesiliete muuttuu mahdollisuudeksi.

Diagnostic and prognostic value of cerebral 31P magnetic resonance spectroscopy in neonates with perinatal asphyxia.

The impact of depressed neonatal cerebral oxidative phosphorylation for diagnosing the severity of perinatal asphyxia was estimated by correlating the concentrations of phosphocreatine (PCr) and ATP as determined by magnetic resonance spectroscopy with the degree of hypoxic-ischemic encephalopathy (HIE) in 23 asphyxiated term neonates. Ten healthy age-matched neonates served as controls. In patients, the mean concentrations +/- SD of PCr and ATP were 0.99 +/- 0.46 mmol/L (1.6 +/- 0.2 mmol/L) and 0.99 +/- 0.35 mmol/L (1.7 +/- 0.2 mmol/L), respectively (normal values in parentheses). [PCr] and [ATP] correlated significantly with the severity of HIE (r = 0.85 and 0.9, respectively, p < 0.001), indicating that the neonatal encephalopathy is the clinical manifestation of a marred brain energy metabolism. Neurodevelopmental outcome was evaluated in 21 children at 3, 9, and 18 mo. Seven infants had multiple impairments, five were moderately handicapped, five had only mild symptoms, and four were normal. There was a significant correlation between the cerebral concentrations of PCr or ATP at birth and outcome (r = 0.8, p < 0.001) and between the degree of neonatal neurologic depression and outcome (r = 0.7). More important, the outcome of neonates with moderate HIE could better be predicted with information from quantitative 31P magnetic resonance spectroscopy than from neurologic examinations. In general, the accuracy of outcome predictability could significantly be increased by adding results from 31P magnetic resonance spectroscopy to the neonatal neurologic score, but not vice versa. No correlation with outcome was found for other perinatal risk factors, including Apgar score.

Cenomanian-Turonian transition in a shallow water sequence of the Sinai, Egypt

Environmental and depositional changes across the Late Cenomanian oceanic anoxic event (OAE2) in the Sinai, Egypt, are examined based on biostratigraphy, mineralogy, delta(13)C values and phosphorus analyses. Comparison with the Pueblo, Colorado, stratotype section reveals the Whadi El Ghaib section as stratigraphically complete across the late Cenomanian-early Turonian. Foraminifera are dominated by high-stress planktic and benthic assemblages characterized by low diversity, low-oxygen and low-salinity tolerant species, which mark shallow-water oceanic dysoxic conditions during OAE2. Oyster biostromes suggest deposition occurred in less than 50 m depths in low-oxygen, brackish, and nutrient-rich waters. Their demise prior to the peak delta(13)C excursion is likely due to a rising sea-level. Characteristic OAE2 anoxic conditions reached this coastal region only at the end of the delta(13)C plateau in deeper waters near the end of the Cenomanian. Increased phosphorus accumulations before and after the delta(13)C excursion suggest higher oxic conditions and increased detrital input. Bulk-rock and clay mineralogy indicate humid climate conditions, increased continental runoff and a rising sea up to the first delta(13)C peak. Above this interval, a dryer and seasonally well-contrasted climate with intermittently dry conditions prevailed. These results reveal the globally synchronous delta(13)C shift, but delayed effects of OAE2 dependent on water depth.

Rasvaisten ja öljyisten jätevesien biologisen puhdistuksen mallinnus

Työn kirjallisuusosassa on tarkasteltu rasvaistenjätevesien puhdistuksessa käytettyjä perinteisiä käsittelymenetelmiä ja ultrasuodatusta. Perinteisiä rasvaisten jätevesien käsittelymenetelmiä ovat muun muassalaskeutus, flotaatio, hydrosykloni, pisarakoon kasvattaminen suodatus sekä biologinen käsittely. Lisäksi happohydrolyysia voidaan soveltaa edellä mainittujen menetelmien esikäsittelynä. Perinteisten puhdistusmenetelmien käyttöä rajoittavatniiden tehottomuus emulgoituneen ja liukoisen öljyn poistossa. Tämä sekä kiristyneet päästövaatimukset ja kalvotekniikan nopea kehittyminen ovat lisänneet kiinnostusta kalvotekniikkaan. Työn soveltavassa osassa on tarkasteltu rasvojen mahdollisesti aiheuttamia ongelmia Porvoon jalostamon kemiallisessa ja biologisessa puhdistuksessa. Rasvaisia jätevesiä muodostuu biodieselin valmistuksessa, jossa rasvoja käytetään syöttöaineena. Vertailtaessa jalostamon vesilaitoksen nykyisiä olosuhteita ja rasvojen käsittelyn vaatimia olosuhteita havaitaan, että optimiolosuhteet ovat melko lähellä toisiaan ja rasvaisten jätevesien mukana tulevat fosfori-, typpi- ja COD-kuormat melko pieniä. Suurimmat mahdolliset rasvojen aiheuttamat ongelmat syntyvät aktiivilietelaitoksella, jossa kevyt pinnalle nouseva rasva nostaa mukanaan lietettä. Rasvat ja rasvahapot myös lisäävät rihmamaisten bakteerien kasvua, joiden runsas esiintyminen aiheuttaa huonosti laskeutuvaa lietettä, eli paisuntalietettä. Rasvaisten vesien aiheuttamaa kuormitusta aktiivilieteprosessiin on tarkasteltu Activated sludge Model No. 3:n ja bio-P fosforin poisto moduuliin pohjautuvan Excel-taulukkolaskentamallin avulla. Pohjana työssä on käytetty Tuomo Hillin vuonna 2002 diplomityönä tekemää taulukkolaskentamallia. Työssä on esitelty kaikki mallin kannalta oleelliset yhtälöt ja parametrit. Tämän tutkimuksen perusteella mallin käytettävyyttä rajoittaa se, että sitä ei ole kalibroitu Porvoon jalostamolle. Kalibroimattomalla mallilla voidaan saada vain suuntaa antavia tuloksia.

Analytical methods for the quantitative determination of selective serotonin reuptake inhibitors for therapeutic drug monitoring purposes in patients.

Five selective serotonin reuptake inhibitors (SSRIs) have been introduced recently: citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline. Although no therapeutic window has been defined for SSRIs, in contrast to tricyclic antidepressants, analytical methods for therapeutic drug monitoring of SSRIs are useful in several instances. SSRIs differ widely in their chemical structure and in their metabolism. The fact that some of them have N-demethylated metabolites, which are also SSRIs, requires that methods be available which allow therapeutic drug monitoring of the parent compounds and of these active metabolites. most procedures are based on prepurification of the SSRIs by liquid-liquid extraction before they are submitted to separation by chromatographic procedures (high-performance liquid chromatography, gas chromatography, thin layer chromatography) and detection by various detectors (UV, fluorescence, electrochemical detector, nitrogen-phosphorus detector, mass spectrometry). This literature review shows that most methods allow quantitative determination of SSRIs in plasma, in the lower ng/ml range, and that they are, therefore, suitable for therapeutic drug monitoring purposes of this category of drugs.

Decomposition of Ruppia cirrhosa (Petagna) Grande in the sediment of a coastal lagoon

The decomposition process of Ruppia cirrhosa was studied in a Mediterranean coastal lagoon in the Delta of the River Ebro (NE Spain). Leaves and shoots of Ruppia were enclosed in 1 mm-mesh and 100 pm-mesh litter bags to ascertain the effect of detritivores, macroinvertebrates, and bacteria and fungi, respectively. Changes in biomass and carbon, and, nitrogen and phosphorus concentrations in the detritus were studied at the sediment-water interface and in the sediment. Significant differences in biomass decay were observed between the two bag types. Significant differences in decomposition were observed between the two experimental conditions studied using 100 pm-mesh bags. These differences were not significant when using the 1 mm-mesh bags. The carbon content in the detritus remained constant during the decomposition process. The percentage of nitrogen increased progressively from an initial 2.4 % to 3 %. The percentage of phosphorus decreased rapidly during the first two days of decomposition from an initial 0.26 % to 0.17 %. This loss is greater in the sediment than in the water column or at the sediment-water interface. From these results we deduce that the activity of microorganisms seems to be more important in the sediment than in the water-sediment interface, and that grazing by macroinvertebrates has less importance in the sediment than in the water column.