Inhibitory effect of gallic acid and its esters on 2,2'-azobis(2-amidinopropane)hodrpchloride (AAPH)-induced hemolysis and depletion of intracellular glutathione in erythrocytes

The protective effect of gallic acid and its esters, methyl, propyl, and lauryl gallate, against 2,2'-azobis(2-amidinopropane)hydrochloride (AAPH)-induced hemolysis and depletion of intracellular glutathione (GSH) in erythrocytes was studied. The inhibition of hemolysis was dose-dependent, and the esters were significantly more effective than gallic acid. Gallic acid and its esters were compared with regard to their reactivity to free radicals, using the DPPH and AAPH/pyranine free-cell assays, and no significant difference was obtained. Gallic acid and its esters not only failed to inhibit the depletion of intracellular GSH in erythrocytes induced by AAPH but exacerbated it. Similarly, the oxidation of GSH by AAPH or horseradish peroxidase/H(2)O(2) in cell-free systems was exacerbated by gallic acid or gallates. This property could be involved in the recent findings on pro-apoptotic and pro-oxidant activities of gallates in tumor cells. We provide evidence that lipophilicity and not only radical scavenger potency is an important factor regarding the efficiency of antihemolytic substances.

Oxidation of amino acids by chlorpromazine cation radical and co-catalysis by chlorpromazine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fungicidas de efeitos fisiológicos no metabolismo e desenvolvimento de plantas de melão rendilhado sob cultivo protegido

Pós-graduação em Agronomia (Horticultura) - FCA

Resposta enzimática, fisiológica e produtiva do tomateiro e desempenho de porta enxertos resistentes à murcha bacteriana

Pós-graduação em Agronomia (Horticultura) - FCA

Quantificação do fator de crescimento semelhante a insulina I (IGF-I) em plasma bovino por ELISA

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da deficiência ou suplementação de zinco sobre a hepatocarcinogênese química em camundongos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ação de fungicidas no desenvolvimento de plantas de tomate, em condições de ambiente protegido

Pós-graduação em Agronomia (Horticultura) - FCA

Caracterização fisiológica, bioquímica e anatômica em genótipos de algodoeiro herbáceo (gossypium hirsutum l.var. latifolium hutch) em função da aplicação de cloreto de mepiquat

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reguladores vegetais e nutrientes minerais no metabolismo de plantas de tomateiro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Adubação potássica na produção e qualidade pós-colheita do rabanete

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da suplementação de açaí (Euterpe oleracea Mart.) na ração sobre remodelação cardíaca em ratos expostos à fumaça do cigarro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mecanismos de ação antioxidante no estado de pós-hipóxia induzida em Phrynops geoffroanus (Testudines:Chelidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da suplementação de açaí (Euterpe oleracea Mart.) na ração sobre a remodelação cardíaca em ratos submetidos a infarto agudo do miocárdio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da temperatura nas respostas aos biocidas diazinon e carbaril em Dilocarcinus pagei Stimpson, 1861

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Role of ATM and PTEN in prostatic carcinogenic dog prostate: validation of aCGH results

The canis lupus familiares is the only species besides human that spontaneously develop prostatic carcinoma (PCa). In addition, the metastatic sites are similar to those frequently reported in men. For these reasons, the dog is the best natural model to study the molecular mechanisms in PCa development providing a natural animal model for treatment by molecular targets. Previously, we investigated copy number alterations by arrayCGH (Canine Genome CGH Microarray 4x44K-G2519F, Agilent Technologies) in canine prostatic lesions: 3 benign prostatic hyperplasias (BPH), 4 proliferative inflammatory atrophies (PIA), and 14 PCa. Five histologically normal prostatic tissues were used as reference. Genomic alterations were evaluated using Genomic Workbench Standard Edition 5.0.14. This previous study revealed significant copy number losses of Atm and Pten exclusively in PCa. In the present study, ATM and PTEN immunoexpression were investigated using a tissue microarray (TMA) containing 149 canine prostatic paraffin-embedded lesions (BPH, PIA and PCa) collected from 67 animals. Immunohistochemical reactions were performed using the polyclonal rabbit antibody anti-PTEN (Santa Cruz Biotech, 1:50) and anti-ATM (Abcam, 1:50). The sections were developed with diaminobenzidine (DAB) and peroxidase. The immunohistochemical staining was assessed in each core by the distribution of positive cells for each antibody per lesion (score 1: <25% cells positive, 2: 26% to 50%, 3: being 51% and 75% and 4:> 75%) and intensity (1: weak, 2: moderate, 3: intense). Chi-square or Fisher exact test was used to determine the association between the categorical variables using GraphPad Prism 5 (GraphPad Software Inc., La Jolla, CA). Distribution of positive cells did not differ among lesions. PCa and PIA showed more samples with weak intensity for ATM when compared to normal prostatic tissue and BPH (PCa: p=0,032 and PIA: p=0,025). Benign prostatic hyperplasia and normal samples presented intense PTEN immunostaining than PCa (p=0,021) and PIA (p=0,0013). These results suggest that ATM and PTEN proteins expression in canine prostatic carcinoma are downregulated possibly by copy number losses. These findings are similar from those described in prostate carcinomas from human corroborating for the use of dogs as a natural model to study prostatic disease in men.