961 resultados para network theory and analysis
Filtro por publicador
- JISC Information Environment Repository (2)
- Repository Napier (1)
- Aberystwyth University Repository - Reino Unido (3)
- Academic Research Repository at Institute of Developing Economies (2)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (5)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (3)
- Applied Math and Science Education Repository - Washington - USA (1)
- Aquatic Commons (13)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (2)
- Archive of European Integration (5)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (11)
- Aston University Research Archive (20)
- Biblioteca Digital da Câmara dos Deputados (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (1)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (5)
- Boston University Digital Common (5)
- Brock University, Canada (8)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- CaltechTHESIS (15)
- Cambridge University Engineering Department Publications Database (88)
- CentAUR: Central Archive University of Reading - UK (41)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (58)
- Cochin University of Science & Technology (CUSAT), India (27)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (2)
- CORA - Cork Open Research Archive - University College Cork - Ireland (11)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (3)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (36)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (3)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (11)
- DigitalCommons@The Texas Medical Center (4)
- DigitalCommons@University of Nebraska - Lincoln (2)
- Diposit Digital de la UB - Universidade de Barcelona (2)
- DRUM (Digital Repository at the University of Maryland) (4)
- Duke University (3)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (1)
- FAUBA DIGITAL: Repositorio institucional científico y académico de la Facultad de Agronomia de la Universidad de Buenos Aires (2)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Glasgow Theses Service (1)
- Greenwich Academic Literature Archive - UK (8)
- Helda - Digital Repository of University of Helsinki (18)
- Indian Institute of Science - Bangalore - Índia (73)
- Instituto Politécnico do Porto, Portugal (3)
- Massachusetts Institute of Technology (4)
- Ministerio de Cultura, Spain (10)
- National Center for Biotechnology Information - NCBI (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (9)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (159)
- Queensland University of Technology - ePrints Archive (174)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (2)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (9)
- Research Open Access Repository of the University of East London. (3)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (2)
- SAPIENTIA - Universidade do Algarve - Portugal (2)
- School of Medicine, Washington University, United States (1)
- Scielo Uruguai (1)
- Universidad del Rosario, Colombia (5)
- Universidad Politécnica de Madrid (3)
- Universidade de Lisboa - Repositório Aberto (1)
- Universitat de Girona, Spain (6)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (4)
- Université de Lausanne, Switzerland (1)
- Université de Montréal, Canada (12)
- University of Connecticut - USA (1)
- University of Michigan (10)
- University of Queensland eSpace - Australia (12)
- University of Southampton, United Kingdom (11)
- University of Washington (1)
- WestminsterResearch - UK (1)
Resumo:
We have developed a new simple method for transport, storage, and analysis of genetic material from the corals Agaricia agaricites, Dendrogyra cylindrica, Eusmilia ancora, Meandrina meandrites, Montastrea annularis, Porites astreoides, Porites furcata, Porites porites, and Siderastrea siderea at room temperature. All species yielded sufficient DNA from a single FTA(R) card (19 mug-43 ng) for subsequent PCR amplification of both coral and zooxanthellar DNA. The D1 and D2 variable region of the large Subunit rRNA gene (LSUrDNA) was amplified from the DNA of P. furcata and S. siderea by PCR. Electrophoresis yielded two major DNA bands: an 800-base pair (bp) DNA, which represented the coral ribosomal RNA (rRNA) gene, and a 600-bp DNA, which represented the zooxanthellar srRNA gene. Extraction of DNA from the bands yielded between 290 mug total DNA (S. siderea coral DNA) and 9 mug total DNA (P. furcata zooxanthellar DNA). The ability to transport and store genetic material from scleractinian corals without resort to laboratory facilities in the field allows for the molecular Study of a far wider range and variety of coral sites than have been studied to date. (C) 2003 Elsevier Science B.V. All rights reserved.