Direct Observation of Millisecond to Second Motions in Proteins by Dipolar CODEX NMR Spectroscopy

We present a site-resolved study of stow (ms to s) motions in a protein in the solid (microcrystalline) state performed with the use of a modified version of the centerband-only detection of exchange (CODEX) NMR experiment. CODEX was originally based on measuring changes in molecular orientation by means of the chemical shift anisotropy (CSA) tensor, and in our modification, angular reorientations of internuclear vectors are observed. The experiment was applied to the study of stow (15)N-(1)H motions of the SH3 domain of chicken a-spectrin. The protein was perdeuterated with partial back-exchange of protons at labile sites. This allowed indirect (proton) detection of (15)N nuclei and thus a significant enhancement of sensitivity. The diluted proton system also made negligible proton-driven spin diffusion between (15)N nuclei, which interferes with the molecular exchange (motion) and hampers the acquisition of dynamic parameters. The experiment has shown that approximately half of the peaks in the 2D (15)N-(1)H correlation spectrum exhibit exchange in a different extent. The correlation time of the slow motion for most peaks is 1 to 3 s. This is the first NMR study of the internal dynamics of proteins in the solid state on the millisecond to second time scale with site-specific spectral resolution that provides both time-scale and geometry information about molecular motions.

pH-Sensitive Binding of Cytochrome c to the Inner Mitochondrial Membrane. Implications for the Participation of the Protein in Cell Respiration and Apoptosis

Cytochrome c exhibits two positively charged sites: site A containing lysine residues with high pK(a) values and site L containing ionizable groups with pK(aobs),values around 7.0. This protein feature implies that cytochrome c can participate in the fusion of mitochondria and have its detachment from the inner membrane regulated by cell acidosis and alkalosis. In this study, We demonstrated that both horse and tuna cytochrome c exhibited two types of binding to inner mitochondrial membranes that contributed to respiration: a high-affinity and low-efficiency pi-I-independent binding (microscopic dissociation constant K(sapp2), similar to 10 nM) and a low-affinity and high-efficiency pH-dependent binding that for horse cytochrome c had a pK(a) of similar to 6.7. For tuna cytochrome c (Lys22 and His33 replaced with Asn and Trp, respectively), the effect of pH on K(sapp1), was less striking than for the horse heme protein, and both tuna and horse cytochrome c had closed K(sapp1) values at pH 7.2 and 6.2, respectively. Recombinant mutated cytochrome c H26N and H33N also restored the respiration of the cytochrome c-depleted mitoplast in a pH-dependent manner. Consistently, the detachment of cytochrome c from nondepleted mitoplasts was favored by alkalinization, suggesting that site Lionization influences the participation of cytochrome c in the respiratory chain and apoptosis.

Occupational airborne contamination in south Brazil: 1. Oxidative stress detected in the blood of coal miners

Reactive oxygen species and nitrogen species have been implicated in the pathogenesis of coal dust-induced toxicity. The present study investigated several oxidative stress biomarkers (Contents of lipoperoxidation = TBARS, reduced = GSH, oxidized = GSSG and total glutathione = TG, alpha-tocopherol, and the activities of glutathione S-transferase = GST, glutathione reductase = GR, glutathione peroxidase = GPx, catalase = CAT and superoxide dismutase = SOD), in the blood of three different groups (n = 20 each) exposed to airborne contamination associated with coal mining activities: underground workers directly exposed, surface workers indirectly exposed, residents indirectly exposed (subjects living near the mines), and controls (non-exposed subjects). Plasma TBARS were increased and whole blood TG and GSH levels were decreased in all groups compared to controls. Plasma alpha-tocopherol contents showed approximately half the values in underground workers compared to controls. GST activity was induced in workers and also in residents at the vicinity of the mining plant, whilst CAT activity was induced only in mine workers. SOD activity was decreased in all groups examined, while GPx activity showed decreased values only in underground miners, and GR did not show any differences among the groups. The results showed that subjects directly and indirectly exposed to coal dusts face an oxidative stress condition. They also indicate that people living in the vicinity of the mine plant are in health risk regarding coal mining-related diseases.

Raman evidence of the interaction between multiwalled carbon nanotubes and nanostructured TiO(2)

Nanocomposites of carbon nanotubes and titanium dioxide (TiO(2)) have attracted much attention due to their photocatalytic properties. Although many examples in the literature have visualized these nanocomposites by electron microscopic images, spectroscopic characterization is still lacking with regard to the interaction between the carbon nanotube and TiO(2). In this work, we show evidence of the attachment of nanostructured TiO(2) to multiwalled carbon nanotubes(MWNTs) by Raman spectroscopy. The nanostructured TiO(2) was characterized by both full-width at half-maximum (FWHM) and the Raman shift of the TiO(2) band at ca 144 cm(-1), whereas the average diameter of the crystallite was estimated as approximately 7 nm. Comparison of the Raman spectra of the MWNTs and MWNTs/TiO(2) shows a clear inversion of the relative intensities of the G and D bands, suggesting a substantial chemical modification of the outermost tubes due to the attachment of nanostructured TiO(2). To complement the nanocomposite characterization, scanning electronic microscopy and X-ray diffraction were performed. Copyright (C) 2011 John Wiley & Sons, Ltd.

European Solar Engineering School ESES : Past and Future

The European Solar Engineering School ESES is a one-year masters program that started in 1999 at the Solar Energy Research Center SERC, Dalarna University College. It has been growing in popularity over the years, with over 20 students in the current year. Approximately half the students come from Europe, the rest coming from all over the globe. This paper described the contents and experiences from seven years of running the programme and the plans for adapting the programme to the Bologna process. The majority of the students from ESES have found work in the solar industry, energy industry or taken up PhD positions. An alumni group has been started that actively gives support to past, present and potential future students.

Middle permian (Guadalupian) brachiopods from the Xiujimqinqi area, Inner Mongolia, northeast China, and their palaeobiogeographical and palaeogeographical significance

A small brachiopod fauna is described from the lower part of the Xiujimqinqi Formation of the Xiujimqinqi area in central-east Inner Mongolia, northeast China. The age of this fauna is regarded as Wordian (Middle Guadalupian, Middle Permian) by comparison with a similar brachiopod fauna from the Zhesi area of central Inner Mongolia, and by constraints from fusulinaceans associated with the Zhesi fauna. The Xiujimqinqi fauna is typical of mixed Boreal/Palaeoequatorial Middle Permian brachiopod faunas of East Asia. The mixed nature of these faunas is interpreted to have resulted from the combined effects of a middle palaeolatitudinal position, intensified plate convergence between Sino-Korea and Mongolia, and sea surface current connections with both the Arctic Sea in the north and eastern Palaeo-Tethys to the south. Possible Kaninospirifer is reported for the first time from China.

Selection of roost sites by the lesser long-eared bat (Nyctophilus geoffroyi) and Gould`s wattled bat (Chalinolobus gouldii) in south-eastern Australia

Physical characteristics of roost sites used by the lesser long-eared bat Nyctophilus geoffroyi and Gould's wattled bat Chalinolobus gouldii were investigated in a farmland–remnant vegetation mosaic and adjacent forested floodplain in south-eastern Australia. A total of 45 individuals of N. geoffroyi and 27 C. gouldii were fitted with radio-transmitters, resulting in the location of 139 and 89 roosts, respectively. Male N. geoffroyi roosted in trees, fallen and decayed timber and artificial structures. These roosts were low to the ground, mainly under bark and in cracks in timber. Roosts of female N. geoffroyi were located higher above ground, and all within trees. Maternity roosts were predominantly located in large dead trees, approximately twice the diameter of roost trees used by females outside the breeding season. No maternity roosts were found under bark, despite half the roosts used by non-breeding females being located in these situations. Both sexes roosted primarily in dead timber and used cavities where the narrowest dimension of the entrance was 2.5 cm. Most roosts of C. gouldii were in dead spouts on large, live river red gum Eucalyptus camaldulensis trees. Intraspecific differences in roost characteristics were less pronounced for this species. Despite access to the same roosting opportunities, there were marked differences in roost selection between N. geoffroyi and C. gouldii. Both species favoured large diameter trees, but differed significantly for all other measured variables: type of roost structure, condition of roost tree (live or dead), height of roost tree, height of roost, and entrance dimensions. Although these species are among the most widespread bats in Australia and are often considered to be habitat 'generalists', both displayed a high level of discrimination in the roosts used. Clearly, roosting requirements are a complex and important issue in the conservation of even the most common species of bats.

Antitropicality of Pacific fishes : molecular insights

Twenty-one molecular genetic studies of thirteen antitropical Pacific fishes are herein reviewed. High dispersal potentials and Plio-Pleistocene transequatorial divergence are suggested for approximately half of the taxa studied, consistent with movement across the tropics during glacial periods. Divergences within two fish groups were mid-Miocene in age, corresponding to a period suggested for vicariant isolation associated with equatorial warming, but high dispersal potentials complicate the interpretation of biogeographic history. Only one study suggested transequatorial divergence older than 20 million years. There is a greater proportion of Pleistocene transequatorial divergences in the East Pacific than the West Pacific, consistent with the suggestion that conditions in the East Pacific are more amenable to the formation of antitropical distributions. Multiple transequatorial divergences have been observed within at least two groups, and instances of cryptic speciation have been identified twice. Areas for future research concern taxa that differ from the majority studied to date with respect to latitudinal distribution, bathymetry, evolutionary age, and dispersal potential. Molecular characters have demonstrated utility for the study of antitropical fishes, but with limitations.

Validity and reliability of a voice-recognition game analysis system for field sports

The purpose of this study was to assess the ability of observers to use voice-recognition analysis to accurately classify gait transitions and quantify gait durations typical of team games. Inter-rater and intra-rater reliability was also determined. Four males were filmed performing pre-determined gait protocols, each comprising different sequences of walking, jogging. running and sprinting. Two operators independently classified gait transitions and the time spent in each gait was determined by the voice recognition system. All gait modes as measured by trained observers demonstrated statistically significant correlations (p<O.O I) to predetermined measurement criteria. The mean absolute error for all gait transitions was less than half a second (0.32-0.36 5) with the maximum percentage error being approximately 4% for the walk, jog and run gaits and 10% for sprinting. Gait classification error was low at 1-9%. The intra-rater and inter-rater reliability was consistently high ranging from r =' 0.87 to 0.99. In conclusion, observers using voice-recognition software provided valid measures of time spent in each of the four gait categories with 90% or better accuracy achieved.

Healthy revolutions: promoting cycling among women

Issue addressed: Australian women's participation in cycling for transport and recreation is approximately half that of men. These gender differences do not occur in several western European countries. Research is required to investigate the individual, social and environmental determinants of Australian women's participation in cycling for transport and recreation.

Discussion: Few studies have systematically investigated women's perceptions and experiences of cycling and little is known about what motivates and sustains their involvement. Preliminary indications are that, for women, there may be an interest in and capacity to participate in cycling that is not being translated into practice. Safety concerns appear to be a significant deterrent to women cycling. Safety factors have a differential impact on women as they are generally more risk averse than men. Quantitative risk assessments suggest that the risk of injury associated with cycling is small and that the health benefits outweigh the health costs. Cycling promotion campaigns may achieve greater success with women if they enable women to experience cycling in an environment that both is, and is perceived to be, safe and supportive.

Conclusions: Research is needed to determine what strategies are likely to be most effective in promoting cycling among Australian women, as a basis for developing programs, policies and facilities to support women's participation in cycling.

Direct current electric potential in an anisotropic half-space with vertical contact containing a conductive 3D body

Detailed studies of anomalous conductors in otherwise homogeneous media have been modelled. Vertical contacts form common geometries in galvanic studies when describing geological formations with different electrical conductivities on either side. However, previous studies of vertical discontinuities have been mainly concerned with isotropic environments. In this paper, we deal with the effect on the electric potentials, such as mise-`a-la-masse anomalies, due to a conductor near a vertical contact between two anisotropic regions. We also demonstrate the interactive effects when the conductive body is placed across the vertical contact. This problem is normally very difficult to solve by the traditional numerical methods. The integral equations for the electric potential in anisotropic half-spaces are established. Green’s function is obtained using the reflection and transmission image method in which five images are needed to fit the boundary conditions on the vertical interface and the air-earth surface. The effects of the anisotropy of the environments and the conductive body on the electric potential are illustrated with the aid of several numerical examples.

How important are different types of temperate woodlands for ground-foraging birds?

There is widespread concern about population decline in a number of woodland-dependent birds in southern Australia. Of all declining species, approximately half forage on the ground. This study examined the avifaunal assemblages of temperate woodlands of the Northern Plains, Victoria, to investigate the importance of woodland habitats for ground-foraging species. Four main types of woodland were surveyed (white cypress-pine, black box, grey box and river red gum) and, in total, 89 bird species were detected. All four woodland types differed in habitat structure and, in turn, supported significantly different avifaunal assemblages. Forty of the 89 species (45%) foraged, at least in part, on the ground. Species richness and abundance of ground-foragers differed significantly between woodland types, being highest in white cypress-pine and black box. There was a greater richness of ground-foragers during the breeding than non-breeding season, but abundance did not vary seasonally. Overall, ground-foraging birds comprised a greater proportion of species (>55%) and individuals (>60%) in white cypress-pine and black box woodland than in grey box and river red gum (42–48% of species, <50% individuals). Those ground-foragers regarded as declining also occurred in greatest richness in white cypress-pine woodlands, one of the most depleted habitats in the region. The lowest richness of ‘declining’ ground-foraging species was in river red gum woodland, the most widespread woodland type. Throughout Australia, the proportion of ground-foraging species in bird assemblages tends to be greater in temperate, semi-arid or arid woodlands than in moist forests and rainforests. However, in many regions woodland habitats are severely depleted and their open ground layer is particularly vulnerable to degradation. The extent of suitable habitat for ground-foraging birds in temperate woodlands may be much less than is apparent from current measures of tree cover. Sustainable management of drier (non-riverine) temperate woodlands is required to conserve this important element of the Australian avifauna.

Fisheries and cage culture of three reservoirs in west Java, Indonesia; a case study of ambitious development and resulting interactions

The interactions between cage culture and wild fishery activities in three Indonesian reservoirs, Saguling, Cirata and Jatiluhur, of the greater Ciratum watershed, West Java, were evaluated using historical data and interviews with cage culture operators. In all three reservoirs, cage culture of common carp, Cyprinus carpio L., and later of common carp and Nile tilapia, Oreochromis niloticus (L.), were encouraged as an alternative livelihood for persons displaced by the impoundment. Currently, a two-net culture system, locally known as 'lapis dua', in which in the inner cage (7 × 7 × 3 m) is used for common carp culture and the outer cage (7 × 7 × 5/7 m) is stocked with Nile tilapia, is practised. On average each cage is stocked with  approximately 100 kg fingerlings each of common carp and Nile tilapia. The numbers of cages and production of cultured fish has increased in the reservoirs, but total and per cage production began to decline from about 1995 in Saguling from 2200 kg cage−1 in 1989 to <500 kg cage−1 in 2002, and in Cirata from a peak of approximately 2300 kg cage−1 in 1995 to approximately 400 kg cage−1 in 2002. In Jatiluhur, which has a considerably lower cage density, total fish production and production per cage has increased since 2000, and currently is approximately 4000 kg cage−1, close to production in the early years of cage culture activities. The cage culture operations also resulted in substantial nutrient loading, estimated at 3.2, 15.2 and 3.1 t of nitrogen and 134, 636 and 128 kg of phosphorous per year in the maximum years of production for Saguling, Cirata and Jatiluhur reservoirs, respectively. In later years, when cage culture production was high, fish kills occurred in the cages, and in Jatiluhur reservoir coincided with a dramatic decline in wild fishery catches. An attempt is made to determine the maximum number of cages for each of the reservoirs that will bring long-term sustainability of cage culture operations and the wild fisheries in the three reservoirs.

Study of polycyclic aromatic hydrocarbons emissions from burning of local wood species in Australia

Four kinds of Australian local wood species were burned in a domestic wood heater installed in a laboratory. The selected wood species include pine, red gum, yellow box and sugar gum, that are the most popular domestic fuel wood in Australia. Particulate matter emissions from burning of each load of wood were sampled isokinetically on filter media from the flue by standard stack emission sampling train. The particle laden filters then went for Gas Chromatography/ Mass Spectrometer (GC/MS) analysis to determine polycyclic aromatic hydrocarbons (PAHs) concentrations. The sampling was conducted under two different burning conditions – air inlet of the combustion chamber fully open and half open. Approximately 15 types of PAHs were detected. Emission factors were derived as microgram of PAHs /kg of wood burned. Total particulate emission factors were also obtained from gravimetric measurement before and after the sampling. PAH emission profiles for four species were generated from the results. Comparisons of emission factors have been conducted among different species of wood, as well as under different burning conditions, ie. fast burning and slowing burning. According to the derived emission factors, pine displayed the highest level of PAHs among the four species, followed by red gum and yellow box, whereas sugar gum showed the lowest level of PAHs. Emission factors were compared between each type of wood under two different burning conditions, the slow burning condition, which was air inlet half open, clearly showed higher PAH levels compared to the fast burning condition. Total PAH fractions on particulate matter were calculated and compared among wood types under two burning conditions. During the fast burning condition, red gum and pine have the higher percentage of PAH to total particulate matter emission than sugar gum and yellow box. When changed to slow burning, the PAH fraction on particulate matter are all increased with sugar gum having the largest increase.