Oral colonization by Candida species in AIDS pediatric patients

The aim of this study was to assess the prevalence of factors associated with oral colonization by Candida spp. in pediatric patients with AIDS. The sample comprised of 117 children. Clinical status, medicines in use, and laboratory findings were obtained from hospital records; sociodemographic data were given by relatives. A dental examination assessed the prevalence of dental caries. The prevalence of oral colonization by Candida was 62%. Only seven children presented clinical manifestation of oral candidosis despite their high viral load index and low-for-age CD4 count. Candida colonization was directly associated with frequent use of antibiotics (prevalence ratio [PR] = 1.44), sulfa drugs (PR = 1.23), alteration in the oral mucosa (PR = 1.55), and untreated dental caries (PR = 1.93). It was inversely associated with the use of antiretroviral therapies (PR = 0.65). Candida albicans was the most frequently detected species (80%); phenotypic tests did not detect C. dubliniensis strains. This study observed a low prevalence of Candida-related oral lesions in these patients, which is compatible with the hypothesis that antiretroviral medicines may have contributed to reducing oral manifestations from Candida infection. The high prevalence of Candida colonization in HIV+/AIDS children with untreated dental caries reinforces the importance of oral health care in interdisciplinary health units that assist these patients.

The Nutritional Status of Children and Adolescents with HIV/AIDS on Antiretroviral Therapy

The objective of this cross-sectional study was to assess the nutritional status of children and adolescents with human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) receiving highly active antiretroviral therapy (HAART). One hundred and eighteen subjects aged 6-19 years attending an outpatient clinic in Sao Paulo city were involved in the study. The following anthropometric measurements were assessed: weight, height, waist circumference and triceps and subscapular skinfold thickness. One (0.9%) adolescent was diagnosed with abdominal obesity based on waist circumference measurement; three (2.5%) adolescents were obese based on subscapular skinfold thickness. According to the body mass index, the population studied was mainly eutrophic. The prevalence of fat redistribution, a characteristic of patients with HIV/AIDS under HAART, was low. We advise the development of further studies to assess the nutritional status of children and adolescents with HIV/AIDS using anthropometric measurements as well as computed tomography to detect fat redistribution.

Diet quality of persons living with HIV/AIDS on highly active antiretroviral therapy

Background This study sought to assess the diet quality of individuals living with HIV/AIDS who were receiving antiretroviral therapy in Sao Paulo Brazil,. Methods This cross-sectional study involved 56 HIV-infected adults. Demographic and anthropometric data were collected, and diet quality was measured using the Healthy Eating Index (HEI), modified for Brazilians, which included ten components: adequacy of intake of six different food groups, total fat, cholesterol, dietary fibre and dietary variety. Results Among the individuals assessed, 64.3% of the participants had a diet needing improvement, while 8.7% had a poor diet. The overall HEI score was 68.3 points (SD = 14.9). Mean scores were low for fruits, vegetables, dairy products and dietary fibre; and high for meats and eggs, total fat and cholesterol. The overall HEI score was higher among individuals who were not overweight (P = 0.003), who were also more likely to achieve dietary goals for dairy products (P = 0.039) and grains (P = 0.005). Conclusion Most of these adults living with HIV/AIDS had diets that required improvement, and being overweight was associated with poorer diet quality. Nutritional interventions aimed at maintaining healthy body weight and diet should be taken into account in caring for HIV-infected people.

Molecular characterization of human polyomavirus JC in Brazilian AIDS patients with and without progressive multifocal leukoencephalopathy

Background: JC virus (JCV), the causative agent of progressive multifocal leukoencephalopathy (PML), is classified in 8 different genotypes. Previous reports have suggested a positive association between specific genotypes and PML. Objective: To compare genotypes and adaptive mutations of JCV strains from Brazilian AIDS patients with and without PML. Study design: The VP1 region of JCV was amplified by polymerase chain reaction from cerebrospinal fluid samples from 51 patients with PML and from urine samples of 47 patients with AIDS without central nervous system disease. Genotyping was done by phylogenetic analysis. Amino acid replacement and selection pressures were also investigated. Results: JCV genotype frequency distributions showed that genotypes 2 (32.7%), 1 (26.5%) and 3 (23.5%) were the most prevalent. Genotype 1 had a positive association (p < 0.0001) and genotype 3 showed an inverse association (p < 0.001) with PML. A previously undescribed point mutation at residue 91 (L/I or L/V) and (L/P), non-genotype-associated, was found in 5/49 (10.2%) and 2/47 (4.3%) JCV sequences from PML and non-PML patients, respectively. This mutation was under positive selection only in PML patients. A previously described substitution of T-A in position 128 showed a significant difference between PML and non-PML cases (70% versus 16%, respectively, p < 0.0005). Conclusion: In Brazilian patients with AIDS, JCV genotype 1 showed a strong association with PML (p < 0.0001) and JCV genotype 3 showed an inverse association with PML. The possible association of aminoacids substitution in residues 91 and 128 with PML in patients with AIDS must be further investigated. (C) 2010 Elsevier B.V. All rights reserved.

Vårdpersonalens kunskaper om och attityder till hiv/aids samt hur omvårdnaden påverkasEn systematisk litteraturstudie.

Syftet med denna litteraturstudie var att beskriva vårdpersonalens kunskap och attityder till personer med hiv/aids samt hur omvårdnaden av dessa patienter påverkades.Datainsamlingsmetoden var datoriserad och manuell sökning. Kravet på de artiklar som studerades var att de skulle ha vetenskapligt värde, vara skrivna på svenska och engelska samt vara publicerade 1997 eller senare. Totalt studerades 19 kvantitativa och kvalitativa artiklar för att belysa detta ämne. För att kunna bedöma kvaliteten i artiklarna användes två olika granskningsmallar. Studien visade att vårdpersonal överlag har goda kunskaper om hiv/aids beträffande högriskgrupper, sjukdomsförlopp, överföringsmetoder samt betydelse av skyddsåtgärder. Kunskap om hiv och aids spelade en avgörande roll i vårdandet av patienten och dennes anhöriga, samt för att kunna skydda sig själv i den nära vården med patienten. Attityder som var vanligt förekommande hos vårdpersonalen var rädsla, osäkerhet, ovillighet att vårda, samt avsaknad av empati i mötet med hiv/aids patienter. I vår studie framkom vidare att vård av aids sjuka patienter var en bidragande orsak till stress och utbrändhet hos vårdpersonal och påverkande därmed omvårdnadshandlingarna negativt.

Ungdomars kunskap om och attityder till HIV/AIDS samt deras sexuella beteende : en systematisk litteraturstudie

Syftet med denna systematiska litteraturstudie var att ur ett globalt perspektiv undersöka ungdomars kunskaper om HIV/AIDS och vilka faktorer som påverkar kunskapsnivån. Syftet var vidare att ur ett globalt perspektiv undersöka ungdomars attityder till HIV/AIDS samt deras sexuella beteende. Sökning av artiklar har skett via databaserna Blackwell Synergy, ELIN@dalarna samt EBSCO Host. Övrig litteratur söktes manuellt på Internet och bibliotek. Inklusionkriterier var att artiklarna skulle vara publicerade mellan år 2000-2006, ha ett vetenskapligt värde, vara skrivna på det svenska eller engelska språket samt vara primärstudier. Vidare skulle deltagarna i artiklarna utgöras av ungdomar mellan 13-25 år. För att bedöma artiklarnas kvalitet användes två granskningsmallar en för kvalitativa artiklar och en för kvantitativa artiklar. Studiens resultat visade att kunskapsnivån bland ungdomar såg olika ut beroende av kön, ålder, sexuell erfarenhet, antal partners, kultur, ekonomiska förutsättningar, föräldrarnas utbildningsnivå samt boende i tätort eller på landsbygd. Ungdomarna uttryckte en rädsla för att smittas av HIV men ansåg sig inte vara i riskzonen för att smittas. Informationskällor om HIV/AIDS utgjordes främst av media och undervisning i skolan. Åldern för ungdomarnas sexuella debut varierade. Resultatet visade även att ett flertal sexpartners var vanligt förekommande och att kondom sällan användes som skydd vid samlag.