998 resultados para Terapia a Laser de Baixa Intensidade
Resumo:
Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a confocal laser scanning microscope CLSM 410 (Carl Zeiss). The reproductive system presented a single and lobate testis, with intercommunications between the lobes without efferent duct. Supernumerary testicular lobe was amorphous and isolated from the normal ones. Collecting tubules (excretory ducts), followed by the excretory bladder, opening to the external media through the excretory pore, were observed at the posterior extremity of the body. In the digestive tract, a cecal swelling was noted at the junction that originates the single cecum. It was concluded that through confocal laser scanning microscopy, new interpretations of morphological structures of S. mansoni worms could be achieved, modifying adopted and current descriptions. The gonad consists of a single lobed testis, similar to that observed in some trematode species. Moreover, the same specimens can be observed either by BM or CLSM, considering that the latter causes only focal and limited damage in tissue structures.
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Donat lincrement en lesperana de vida, valorem el tractament radical del cncer de prstata en pacients & 70 anys. Lobjectiu s analitzar la incidncia, severitat i maneig de la morbiditat perioperatria de la prostatectomia radical (PR) en funci de la tcnica quirrgica. De 500 PR revisem, retrospectivament, 70 pacients & 70 anys (40 Retropbiques i 30 Laparoscpiques). Sempra la classificaci de Clavien modificada per analitzar les complicacions. La proporci de complicacions s en PRR 57,5% i en PRL 33%. Les complicacions en la PRL sn de menor gravetat. Els beneficis atributs a la laparoscpia es mantenen en pacients dedat avanada.
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Abans de la terpia fetal, ms del 90 % dels casos de fetus amb malformacions pulmonars, basaments pleurals i hidrops morien. Avui en dia, grcies a la terpia fetal i, mes concretament, a la collocaci de shunts toracoamnitics aquesta supervivncia ha augmentat notablement. Des del 2003, el nostre centre ha realitzat aquest procediment a 25 fetus amb resultats favorables ( una supervivncia total del 62.5%) i una baixa taxa de complicacions. Levoluci a llarg termini dels pacient que han sobreviscut s favorable i el seu estudi ens ha perms identificar alguns factors pronstic a tenir en compte.
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OBJECTIVE: The purpose of this study was to compare the use of different variables to measure the clinical wear of two denture tooth materials in two analysis centers. METHODS: Twelve edentulous patients were provided with full dentures. Two different denture tooth materials (experimental material and control) were placed randomly in accordance with the split-mouth design. For wear measurements, impressions were made after an adjustment phase of 1-2 weeks and after 6, 12, 18, and 24 months. The occlusal wear of the posterior denture teeth of 11 subjects was assessed in two study centers by use of plaster replicas and 3D laser-scanning methods. In both centers sequential scans of the occlusal surfaces were digitized and superimposed. Wear was described by use of four different variables. Statistical analysis was performed after log-transformation of the wear data by use of the Pearson and Lin correlation and by use of a mixed linear model. RESULTS: Mean occlusal vertical wear of the denture teeth after 24 months was between 120μm and 212μm, depending on wear variable and material. For three of the four variables, wear of the experimental material was statistically significantly less than that of the control. Comparison of the two study centers, however, revealed correlation of the wear variables was only moderate whereas strong correlation was observed among the different wear variables evaluated by each center. SIGNIFICANCE: Moderate correlation was observed for clinical wear measurements by optical 3D laser scanning in two different study centers. For the two denture tooth materials, wear measurements limited to the attrition zones led to the same qualitative assessment.
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Inhibition of vascular endothelial growth factor (VEGF) has become the standard of care for patients presenting with wet age-related macular degeneration. However, monthly intravitreal injections are required for optimal efficacy. We have previously shown that electroporation enabled ciliary muscle gene transfer results in sustained protein secretion into the vitreous for up to 9 months. Here, we evaluated the long-term efficacy of ciliary muscle gene transfer of three soluble VEGF receptor-1 (sFlt-1) variants in a rat model of laser-induced choroidal neovascularization (CNV). All three sFlt-1 variants significantly diminished vascular leakage and neovascularization as measured by fluorescein angiography (FA) and flatmount choroid at 3 weeks. FA and infracyanine angiography demonstrated that inhibition of CNV was maintained for up to 6 months after gene transfer of the two shortest sFlt-1 variants. Throughout, clinical efficacy was correlated with sustained VEGF neutralization in the ocular media. Interestingly, treatment with sFlt-1 induced a 50% downregulation of VEGF messenger RNA levels in the retinal pigment epithelium and the choroid. We demonstrate for the first time that non-viral gene transfer can achieve a long-term reduction of VEGF levels and efficacy in the treatment of CNV.Gene Therapy advance online publication, 27 June 2013; doi:10.1038/gt.2013.36.
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Malnutrition hampers the course of schistosomiasis mansoni infection just as normal growth of adult worms. A comparative morphometric study on adult specimens (male and female) recovered from undernourished (fed with a low protein diet - regional basic diet) and nourished (rodent commercial laboratory food, NUVILAB) white mice was performed. Tomographic images and morphometric analysis of the oral and ventral suckers, reproductive system and tegument were obtained by means of confocal laser scanning microscopy. Undernourished male specimens presented smaller morphometric values (length and width) of the reproductive system (first, third and last testicular lobes) and thickness of the tegument than controls. Besides that, it was demonstrated that the dorsal surface of the male worms bears large tubercles unevenly distributed, but kept grouped and flat. At the subtegumental region, vacuolated areas were detected. It was concluded that the inadequate nutritional status of the vertebrate host has a negative influence mainly in the reproductive system and topographical somatic development of male adult Schistosoma mansoni, inducing some alterations on the structure of the parasite.
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La tasca investigadora presentada en aquesta memria s'ha centrat en les fonts galctiques de raigs gamma de molt alta energia LS I +61 303, HESS J1708-410 i HESS J1858+020. La primera s una binria de raigs gamma molt estudiada, formada per una estrella massiva i un objecte compacte. S'ha proposat un escenari on l'objecte compacte seria un plsar jove, i la interacci del seu vent amb el vent de l'estrella generaria els raigs gamma. De totes formes, no s'ha detectat polsos procedents d'aquest putatiu plsar. L'investigador va realitzar observacions en fase a 1280 MHz amb el radiotelescopi GMRT, sense trobar-hi polsos, cosa que implica un estricte lmit superior de 0,38 mJy a la densitat mitjana de flux polsat en un putatiu plsar amb un perode major que 2 millisegons en el sistema binari LS I +61 303. Per altra banda, HESS J1708-410 i HESS J1858+020 sn dues fonts esteses de raigs gamma de molt alta energia de les quals no es coneix cap contrapart a d'altres longituds d'ona. L'investigador les va observar amb el GMRT, quatre vegades HESS J1708-410 (dues a 610 MHz i dues a 1400 MHz) i dues vegades HESS J1858+020 (una a cada freqncia). En les imatges realitzades amb aquestes dades no hi ha emissi estesa coincident amb les regions d'emissi de raigs gamma. HESS J1858+020 se solapa parcialment amb una font estesa que podria ser un SNR. De confirmar-se la falta de contrapartida rdio de HESS J1708-410, estarem parlant d'un accelerador hadrnic extraordinriament eficient, d'una classe desconeguda fins ara.
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Objectiu. Analitzar limpacte de la immunoquimioterpia antilimfomatosa en lestat immunolgic i virolgic a llarg termini dels pacients amb leucmia/limfoma de Burkitt i infecci pel virus de la immunodeficincia humana (VIH). Pacients y mtode. Dels 25 pacients VIH positius inclosos en lassaig clnic BURKIMAB del grup cooperatiu PETHEMA entre juliol de 2003 i febrer de 2009, 14 van tenir un seguiment superior a un any. Tots ells van rebre terpia antirretroviral de gran activitat durant i desprs de la immunoquimioterpia. En aquests pacients es va avaluar la resposta virolgica (CV & 50 cpies/mL) i immunolgica (limfcits CD4 & 200/L), aix com els events tardans relacionats amb el VIH. Resultats. Tretze pacients (93%) eren homes, amb una mediana dedat de 37 (extrems 31-54) anys. El seguiment medi dels 14 pacients va ser de 37 mesos, (extrems 13-43). Tres (21%) presentaven una resposta immunolgica i virolgica al TARGA en el moment del diagnstic de la LB i tots ells van romandre en aquesta situaci desprs de completar la immunoquimioterpia i durant el seguiment a llarg termini. Quatre (29%) pacients en resposta immunolgica per amb crrega viral detectable en el moment del diagnstic van assolir la resposta virolgica i la van mantenir. Finalment, 5/7 (71%) pacients amb infecci pel VIH no controlada en el moment del diagnstic van assolir i mantenir la resposta immunolgica i virolgica desprs de la immunoquimioterpia. Desprs dun seguiment de 496 pacients-any no sha reportat cap prdua de resposta al tractament amb TARGA. Shan diagnosticat 4 infeccions associades al VIH: infeccions por micobactries (2), gastroenteritis de repetici per Blastocystis hominis i sfilis. Un pacient va desenvolupar un sarcoma de Kaposi. Fins la data no sha produt cap mort en aquests pacients. Conclusions: La immunoquimioterpia en els pacients afectes de LB i infecci pel VIH no ha afectat negativament a la resposta virolgica ni immunolgica obtinguda amb el TARGA y sha associat a una baixa incidncia devents relacionats amb el VIH.
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Dels pacients amb DM1 diagnosticats entre 1985-1994 en el nostre centre, vrem seleccionar 147 amb seguiment ininterromput des del debut i durant ms de 10 anys. Vrem revisar les dades registrades anualment relacionades amb el tractament, control glicmic i risc cardiovascular, amb els objectius d'avaluar el Grau de control, l'existncia de factors predictors, la prevalena de complicacions tardanes i la seva relaci amb el control glucmic. Vrem objectivar que la insulinoterpia intensiva permetia mantenir un control glucmic adequat, i que el grau de control a l'any del diagnstic predeia el control posterior. La prevalena de complicacions va ser inferior a la descrita prviament.
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Schistosoma mansoni adult worms with genital anomalies isolated from Nectomys squamipes (Muridae: Sigmodontinae) were studied by confocal laser scanning microscopy under the reflected mode. One male without testicular lobes (testicular agenesia/anorchism) and two females, one with an atrophied ovary and another with 17 uterine eggs, were identified. The absence of testicular lobes occurred in a worm presenting otherwise normal male adult characteristics: tegument, tubercles and a gynaecophoric canal with spines. In both female specimens the digestive tube showed a vacuolated appearance, and the specimen with supernumerary uterine eggs exhibited a developing miracidium and an egg with a formed shell. The area of the ventral sucker was similar in both specimens however the tegument thickness, ovary and vitelline glands of the specimen with the atrophied ovary were smaller than those of the one with supernumerary eggs. These reported anomalies in the reproductive system call attention to the need to improve our understanding of genetic regulation and the possible role of environmental influences upon trematode development.
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To compare autofluorescence (AF) images obtained with the confocal scanning laser ophthalmoscope (using the Heidelberg retina angiograph; HRA) and the modified Topcon fundus camera, in a routine clinical setting. A prospective comparative study conducted at the Jules-Gonin Eye Hospital. Fifty-six patients from the medical retina clinic. All patients had complete ophthalmic slit-lamp and fundus examinations, colour and red-free fundus photography, AF imaging with both instruments, and fluorescein angiography. Cataract and fixation were graded clinically. AF patterns were analyzed for healthy and pathological features. Differences of image noise were analyzed by cataract grading and fixation. A total of 105 eyes were included. AF patterns discovered by the retina angiograph and the fundus camera images, respectively, were a dark optic disc in 72% versus 15%, a dark fovea in 92% versus 4%, sub- and intraretinal fluid visible as hyperautofluorescence on HRA images only, lipid exudates visible as hypoautofluorescence on HRA images only. The same autofluorescent pattern was found on both images for geographic atrophy, retinal pigment changes, drusen and haemorrhage. Image noise was significantly associated with the degree of cataract and/or poor fixation, favouring the fundus camera. Images acquired by the fundus camera before and after fluorescein angiography were identical. Fundus AF images differ according to the technical differences of the instruments used. Knowledge of these differences is important not only for correctly interpreting images, but also for selecting the most appropriate instrument for the clinical situation.
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A comparative morphometric study was performed to identify host-induced morphological alterations in Schistosoma mansoni adult worms. A wild parasite population was obtained from a naturally infected rodent (Nectomys squamipes)and then recovered from laboratory infected C3H/He mice. Furthermore, allopatric worm populations maintained for long-term under laboratory conditions in Swiss Webster mice were passed on to N. squamipes. Suckers and genital system (testicular lobes, uterine egg, and egg spine) were analyzed by a digital system for image analysis. Confocal laser scanning microscopy (CLSM) showed details of the genital system (testicular lobes, vitelline glands, and ovary) and the tegument just below the ventral sucker. Significant morphological changes (p < 0.05) were detected in male worms in all experimental conditions, with no significant variability as assessed by CLSM. Significant changes (p < 0.05) were evident in females from the wild population related to their ovaries and vitelline glands, whereas allopatric females presented differences only in this last character. We conclude that S. mansoni worms present the phenotypic plasticity induced by modifications in the parasite's microenvironment, mainly during the first passage under laboratory conditions.
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Laser desorption ionisation mass spectrometry (LDI-MS) has demonstrated to be an excellent analytical method for the forensic analysis of inks on a questioned document. The ink can be analysed directly on its substrate (paper) and hence offers a fast method of analysis as sample preparation is kept to a minimum and more importantly, damage to the document is minimised. LDI-MS has also previously been reported to provide a high power of discrimination in the statistical comparison of ink samples and has the potential to be introduced as part of routine ink analysis. This paper looks into the methodology further and evaluates statistically the reproducibility and the influence of paper on black gel pen ink LDI-MS spectra; by comparing spectra of three different black gel pen inks on three different paper substrates. Although generally minimal, the influences of sample homogeneity and paper type were found to be sample dependent. This should be taken into account to avoid the risk of false differentiation of black gel pen ink samples. Other statistical approaches such as principal component analysis (PCA) proved to be a good alternative to correlation coefficients for the comparison of whole mass spectra.
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Objective: To discuss the convenience of laser surgery as optimal treatment for melanoma of the oral mucosa.Patients and methods: A retrospective evaluation of four patients with primary oral melanomas treated at a single Cancer Institution in Mexico City.Results: Two patients were treated with resection of the melanoma with CO2 laser together with extraction of the involved dental organs and curettage of the alveolar walls. These two cases had melanoma in situ with multiple isolated foci. The third patient had a lesion with vertical growth, who was submitted to partial maxillectomy along with selective dissection of bilateral neck levels I-V with a negative report and the fourth patient had a history of oral nodular melanoma and presented with lymph node metastasis. According to follow-up status, there was no distant metastasis in any of the patients reported here.Conclusion: In our experience, conservative management with CO2 laser is adequate for melanomas of the oral mucosa with extraction of the dental organs and curettage of the alveoli to achieve complete surgical resection microscopically without sacrifice of the quality of life. Management of the neck is controversial. We recommend selective therapeutic resection of the neck only if it is found to be clinically positive. Elective dissection has not shown to have an impact in overall survival.
