Análise eletroforética de proteínas recombinantes obtidas de Pichia pastoris

A glicerol quinase é uma proteína tetramérica que cataliza a fosforilação do glicerol a glicerol-3-fosfato, composta por quatro subunidades - sua análise eletroforética fornecerá apenas uma banda se for constatada a sua pureza num gel não desnaturante - e sua reação com o glicerol é dependente de magnésio e de ATP. A eletroforese de proteínas utiliza como suporte um gel de poliacrilamida, que é formado pela polimerização de monômeros de acrilamida ao longo da sua cadeia e ligações cruzadas de cadeias pela inclusão de um co-monômero bifuncional apropriado, usualmente a N,N’ – metileno-bis-acrilamida ou apenas Bis . A eletroforese de proteínas mais comum é a que utiliza SDS para um gel de poliacrilamida desnaturante. O SDS é um sal chamado Dodecil Sulfato de Sódio que tem por característica se ligar a cadeia proteína nos resíduos de aminoácidos apolares, deixando com a carga negativa, sendo assim, toda proteína fica com carga total negativa, migrando para o anodo na eletroforese. As técnicas de purificação utilizadas foram ultrafiltração e precipitação com ácido tricloro acético, etanol gelado e sulfato de amônio. A dupla precipitação com etanol resultou na recuperação de maior quantidade de proteínas. A coloração do gel com prata foi mais sensível do que Comassie blue. O gel de eletroforese mostrou quatro bandas, correspondentes às quatro subunidades da glicerol quinase quando revelados com prata em gel de SDS-PAGE.

Análise do conforto térmico da cidade de Mirandópolis/SP em seus espaços abertos

Mirandópolis/SP appeared in 1934 from the railroad “the Brazil Northwest”, that there it served one of the points of its stretch that had as main function to drain the agricultural production northwestern native of São Paulo of that time. The installation of the town happened absent of concerns with initial urbanístico planning, thus modifying its natural landscape and modifying characteristic ambient without compensatory measures aiming at a future expansion of the urban mesh. Fact this that still causes today consequences the quality of life of the people and also the observed thermal comfort later. Ahead of this, the present work had for objective to identify through measurements of temperature and relative humidity of the city of Mirandópolis/SP the differences that in the fabric urban they corroborate for a comfort situation or thermal discomfort to the outdoors in accordance with the Diagram Bioclimático de Olgyay the collection of data was made throughout the month of July of 2011 involving the attainment of information of Temperature and relative humidity of air and questionnaire applied to the passer-bys in the act of the measurements. Also approaching the different thermal dynamic of the zones of housing in relation to the Urban Cannyon that develops the commerce as predominant activity. This research sample that, although a city to be small, the quality of life of its inhabitants is not only guaranteed by the transport or that its thermal comfort reveals inside homogeneous of the urban perimeter, but that it has yes heterogeneidade in the scope of the urban climate in these spaces

Análise da estabilidade dimensional e resistência mecânica em bambu Guadua angustifolia pós-processos de tratamentos químicos e térmico

Nowadays, bamboo is being studied because of their strength properties according with their specific mass and speed of growth, which makes it an important alternative as a new resource that will help reduce pressure on forests and helpping them favoring the minimization of uncontrolled deforestation in many regions of Brazil. This study aimed, in general, to analyze physical and mechanical properties of the material with the divulgation of its potential for industrial application. To do so, in this research were determined in relation to the physical properties, moisture content, dimensional stability and the apparent densities and the mechanics and basic, just a tension parallel to grain, in order to observe the interference of various kinds of treatments (chemical, thermal and natural) on the strength and modulus of elasticity in this request. The species used was the Guadua angustifolia, a species native of Brazil. All tests were performed at Universidade Estadual Paulista - Campus Experimental Itapeva in the laboratory of Materials Properties. The methodology used for testing of moisture, density and tension parallel to grain were based on NBR 7190/1997 for the wood, and dimensional stability tests were based on much the same as in COPANT 462/1972 (South American) . The preservative treatments conducted followed the recommendation of each manufacturer. The values obtained in tests of physical properties were satisfactory especially with respect to density and dimensional stability analyzed by the coefficient of anisotropy, showed that, compared to wood, excellent quality for the shrinkage test, obtaining a coefficient of 1.2. With respect to parallel tensile tests to fibre results showed, in most cases, that test specimens with the presence of us have lower values of resistance and modulus of elasticity when compared with those without us. In the treatment of thermal-treatment there was an apparent treatment there was an apparent increase in...

Análise teórica e experimental de uma ventaneira de alto forno, visando seu comportamento térmico e fluidodinâmico

In this work, it was studied the thermal and fluid dynamic behavior from theorical and experimental point of view of a blast furnace tuyere. The tuyere is responsible for the hot air insuflation into the blast furnace. The parameter used in the comparison was the difference between the cooling water inlet and outlet temperatures. There were used forced convection correlations inside circular sections with adequations for non circular sections. Based on operations dates of flux and thermal loads it was possible to model numerically the tuyere, and, since it was obtained the wall temperatures, estimate the conduction and convection resistances and the heat flux through the walls in contact with the water. The total heat fluxes from wall to water were applied to the energy conservation equation where could be estimated the theorical temperature variation. Compared to the real value, the theorical value presented a difference of 0,2 °C. Considering that the boundary conditions around the tuyere are transitories and that your channels have some rugosity irregularities we can consider the estimation method for cooling system coherent with the real operational parameters

Estudos estruturais com a importina-α e Sequências de Localização Nuclear (NLS) de proteínas envolvidas no metabolismo de Neurospora crassa

Proteínas que apresentam atividades no núcleo e possuem sequência de localização nuclear (NLS) tem seu deslocamento dependente do heterodímero importina-α/β. A importina- (ImpA) é responsável pelo reconhecimento inicial do substrato a ser importado através da interação com os NLS. Os sinais são caracterizados por apresentar um ou mais grupos de aminoácidos básicos, denominados como sequências monopartidas e bipartidas. O fungo Neurospora crassa vem sendo utilizado há mais de 70 anos como organismo modelo em estudos de expressão gênica, desenvolvimento e diferenciação celular, ritmo circadiano, defesa do genoma, bem como outros aspectos da biologia de eucariotos. A presença de um grande número de genes no genoma de N. crassa ainda com funções desconhecidas aponta este organismo como um promissor modelo para o estudo de novos mecanismos genéticos e bioquímicos ainda não identificados. Considerando a importância do metabolismo do glicogênio para os organismos, o presente trabalho teve como objetivo o estudo estrutural de complexos de ImpA com peptídeos NLSs (NCM e NCB) de proteínas envolvidas no metabolismo de glicogênio do fungo N. crassa, utilizando técnicas de cristalografia de proteínas. Monocristais dos complexos ImpA-NCM e ImpA-NCB foram obtidos para a coleta dos dados de difração de raios-X, resultando em dois conjuntos de dados à 2,1Å e 2,45Å de resolução, respectivamente. Após elucidação da estrutura da ImpA, mapas de densidade eletrônica gerados revelaram uma densidade eletrônica no sítio principal de reconhecimento de NLS da ImpA de ambas estruturas, possibilitando modelagem dos peptídeos. Em uma comparação do mapa de densidade eletrônica obtido de ambos complexos com um mapa de uma estrutura nativa de ImpA (70-529) coletada à 2,0Å de resolução, a qual usualmente apresenta um peptídeo “contaminante” no sitio de ligação... (Resumo completo, clicar acesso eletrônico abaixo)

Estudo de causas de falhas em selos mecânicos de bombas centrífugas para circulação de óleo térmico

This study is about a heating line that uses thermal oil. It is located in a facility that produces interlayers used in cars windshields. A plastic resin is melted and is sent to a matrix called DIE where the interlayers are produced. The DIE needs to receive heat from the circulation of thermal oil in four different regions of its interior, to ensure the correct thickness of the interlayer. The thermal energy is provided by electric heaters and the flow of thermal oil is impelled by four mechanically sealed centrifugal pumps (one per region). The key point of this study is the fact that these four pumps of the system have reported successive failures in the mechanical seals. Apparently, a poorly designed project has burdened the system intermittently. The pumps operation condition is located in a region of low efficiency, according to the characteristic curves. This fact is the source of the noticeable reduced time between failures. Changes in the configuration of the facility and in the number of pumps will be proposed, aiming to achieve higher operational efficiency. The proposals will be mathematically analyzed according to the Hydraulic Institute criteria. At the same time, we will also keep focus on an in-depth study of a heating system structure, starting with a detailed approach for each component and discussion about its real need and economic viability. At the end of this paper it is shown that the gain in efficiency achieved with the new proposed configuration reflects not only in the reduction of maintenance costs, but also a potential improvement in energy efficiency. It is shown that these two aspects are closely related and together form the basis for the design of a reliable and efficient pumping system

Ação dos inotrópicos e vasopressores na estabilização hemodinâmica em cães com choque séptico

Septic shock remains one of the most common challenges for the small animal practicing, presenting high mortality rates frequently associated with late identification of this syndrome, as well as an inappropriate treatment. In general, disruption of homeostasis occurs with an intense activation of inflammatory cascade, which leads to a damage to endothelial cells and an exposure to these cytokines, which will result in vasodilation and increased capillary permeability. Thus, there is a drop in blood pressure, even after aggressive fluid resuscitation. Therefore, drugs such as vasopressors, which act by increasing systemic vascular resistance, and inotropes, which have an effect on heart pump, should be administered in order to raise blood pressure, ensuring adequate tissue perfusion. The objective of this review was to gather information about the various drugs used in vasopressors/inotropes therapy, trying to explain the role of each one in different situations, in order to increase the survival rate in dogs affected with septic shock

Estudo sobre fornos de tratamento térmico e propostas de cogeração de energia em usina siderurgica não integrada

Steel industry is a sector heavily dependent on energy, both electrical and thermal. Since the receipt of raw materials to the shipment of finished products to customers, through mergers, casting, rolling, heat treatment, inspection, among others, high amounts of energy are demanded, generating very significant costs to the productive chain in its entirety . Therefore, any alternative that favors the reduction in energy consumption or barateie the cost of this is very welcome. Within this context, this paper aims to make a technical and economic analysis of installing a cogeneration plant in the field rolling in a non-integrated steel mill. Two configurations are proposed plants, with one being the use of heat from waste gases from furnaces existing in the area mentioned and another with the use of heat from waste gases from an internal combustion engine. Both proposals are evaluated technically and later is done the economic analysis, calculating the financial return (pay back) in relation to the investment required, operation and maintenance of the plant

Avaliação da influência da taxa de resfriamento posterior ao tratamento térmico nas propriedades mecânicas do aço API 5L X70

In this work a study about the mechanical properties of the API 5L X70 steel, with or without heat treating, has been made, with the intetion of assess the influence of cooling after the austenitization heat treating by air cooling (normalizing) and a rapid cooling with oil (tempering). This steel is known by high strength and ductility values and it is commonly used in the manufacture of oil pipes. The growing energy demand encouraged the study and manufacture of this material. Although this microalloyed dispense subsequent heat treatings, it was proven that its implementation is very advantageous for this type of application, improving hardness and plastic stability. It was also assessed that the faster the cooling rate is, the better will be these properties

Choque

The shock, now known as a clinical syndrome due to a systemic breakdown in tissue perfusion leading to cellular hypoxia, metabolic changes and consequently organ dysfunction, is a condition that affects both humans and animals and should be classified as an emergency. For its dynamic course the therapy becomes a challenge, all the time time spared in diagnosis and treatment is essential to save the patient's life, so knowledge of its physiopathology can become of great value, and in order to clarify it better, shock has been divided didactically into: cardiogenic, hypovolemic, distributive, and obstructive, so the best approach may be chosen to this situation, noting that the therapy in general is the sum of several procedures that aims to compensate for the animal so that the underlying cause of shock may be treated

Estudo de caso do conforto térmico de dois modelos construtivos em madeira: wood frame e log home

O presente trabalho consiste em demonstrar valores referentes ao conforto ambiental de dois modelos construtivos, sendo o primeiro um edifício já executado e o outro em fase de implantação, ambos no Campus Experimental de Itapeva, aplicando alguns conceitos em habitação mais sustentáveis de madeira na região de Itapeva, estado de São Paulo, Brasil. A edificação construída sedia duas salas de aula a partir da tipologia construtiva wood frame, constituída basicamente de paredes autoportantes compostas de ossatura de madeira tratada de pinus.sp, chapas estruturais em OSB, manta de isolamento térmico/acústico e fechamento interno e externo, respectivamente em gesso e sidding (lambris de madeira). A habitação em fase de início de implantação foi idealizada a partir da tipologia construtiva log-home, e atenderá a necessidade de espaço físico para abrigar o Centro Acadêmico e a empresa-júnior “PROMAD”, ambos do curso de Engenharia Industrial Madeireira do Campus Experimental da UNESP/Itapeva. Esta construção foi elaborada a partir de técnicas de baixo impacto ambiental, levando em consideração os resultados de análise térmica obtidos na edificação anterior. Em ambos modelos construtivos foram empregados recursos renováveis abundantes na região, como a madeira de reflorestamento de Pinus e de Eucalipto. Foi acrescentado ainda ao segundo modelo a utilização de resíduos industriais lignocelulósicos, como rolete de laminação e outros materiais locais disponíveis. A metodologia básica empregada para a obtenção de dados sobre o conforto térmico em relação as edificações foi, para o primeiro caso, a coleta de dados de temperatura e umidade utilizando um termo-higrômetro, a sistematização e análise de dados reais obtidos no interior da edificação. No segundo caso, o dimensionamento do conforto térmico... (Resumo completo, clicar acesso eletrônico abaixo)

Concentrações séricas e peritoneal de proteínas de fase aguda em equinos submetidos à endotoxemia experimental, tratados ou não com lidocaína

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Parâmetros clínicos e laboratoriais relacionados ao prognóstico em cães com gastroenterite hospitalizados

Pós-graduação em Cirurgia Veterinária - FCAV

Síntese, caracterização e estudo do comportamento térmico dos oxanatos de lantanídeos(III) e de ítrio(III), no estado sólido

Pós-graduação em Química - IQ

Estudo da interação não-nativa no enovelamento de proteínas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)