Microcalcifications Detection using PFCM and ANN

This work presents a method to detect Microcalcifications in Regions of Interest from digitized mammograms. The method is based mainly on the combination of Image Processing, Pattern Recognition and Artificial Intelligence. The Top-Hat transform is a technique based on mathematical morphology operations that, in this work is used to perform contrast enhancement of microcalcifications in the region of interest. In order to find more or less homogeneous regions in the image, we apply a novel image sub-segmentation technique based on Possibilistic Fuzzy c-Means clustering algorithm. From the original region of interest we extract two window-based features, Mean and Deviation Standard, which will be used in a classifier based on a Artificial Neural Network in order to identify microcalcifications. Our results show that the proposed method is a good alternative in the stage of microcalcifications detection, because this stage is an important part of the early Breast Cancer detection

Research lines in Hyperthermia at the Bioinstrumentation Laboratory of the Centre for Biomedical Technology

The Bioinstrumentation Laboratory belongs to the Centre for Biomedical Technology (CTB) of the Technical University of Madrid and its main objective is to provide the scientific community with devices and techniques for the characterization of micro and nanostructures and consequently finding their best biomedical applications. Hyperthermia (greek word for “overheating”) is defined as the phenomenon that occurs when a body is exposed to an energy generating source that can produce a rise in temperature (42-45ºC) for a given time [1]. Specifically, the aim of the hyperthermia methods used in The Bioinstrumentation Laboratory is the development of thermal therapies, some of these using different kinds of nanoparticles, to kill cancer cells and reduce the damage on healthy tissues. The optical hyperthermia is based on noble metal nanoparticles and laser irradiation. This kind of nanoparticles has an immense potential associated to the development of therapies for cancer on account of their Surface Plasmon Resonance (SPR) enhanced light scattering and absorption. In a short period of time, the absorbed light is converted into localized heat, so we can take advantage of these characteristics to heat up tumor cells in order to obtain the cellular death [2]. In this case, the laboratory has an optical hyperthermia device based on a continuous wave laser used to kill glioblastoma cell lines (1321N1) in the presence of gold nanorods (Figure 1a). The wavelength of the laser light is 808 nm because the penetration of the light in the tissue is deeper in the Near Infrared Region. The first optical hyperthermia results show that the laser irradiation produces cellular death in the experimental samples of glioblastoma cell lines using gold nanorods but is not able to decrease the cellular viability of cancer cells in samples without the suitable nanorods (Figure 1b) [3]. The generation of magnetic hyperthermia is performed through changes of the magnetic induction in magnetic nanoparticles (MNPs) that are embedded in viscous medium. The Figure 2 shows a schematic design of the AC induction hyperthermia device in magnetic fluids. The equipment has been manufactured at The Bioinstrumentation Laboratory. The first block implies two steps: the signal selection with frequency manipulation option from 9 KHz to 2MHz, and a linear output up to 1500W. The second block is where magnetic field is generated ( 5mm, 10 turns). Finally, the third block is a software control where the user can establish initial parameters, and also shows the temperature response of MNPs due to the magnetic field applied [4-8]. The Bioinstrumentation Laboratory in collaboration with the Mexican company MRI-DT have recently implemented a new research line on Nuclear Magnetic Resonance Hyperthermia, which is sustained on the patent US 7,423,429B2 owned by this company. This investigation is based on the use of clinical MRI equipment not only for diagnosis but for therapy [9]. This idea consists of two main facts: Magnetic Resonance Imaging can cause focal heating [10], and the differentiation in resonant frequency between healthy and cancer cells [11]. To produce only heating in cancer cells when the whole body is irradiated, it is necessary to determine the specific resonant frequency of the target, using the information contained in the spectra of the area of interest. Then, special RF pulse sequence is applied to produce fast excitation and relaxation mechanism that generates temperature increase of the tumor, causing cellular death or metabolism malfunction that stops cellular division

La modernidad arquitectónica en México : una mirada a través del arte y los medios impresos

Esta tesis se basa en la hipótesis de que la modernidad arquitectónica en México no es, como se ha pretendido, una historia homogénea, centrada en un puñado de figuras clave, sino una multiplicidad de narrativas complejas en las cuales el arte y los medios impresos juegan un papel esencial. Por tanto, se propone una nueva mirada sobre la arquitectura del siglo XX en México a partir de la relación con la fotografía, el dibujo, las ideas y los medios. La tesis se plantea con el fin de vincular la arquitectura con los movimientos artísticos relevantes, los autores con las publicaciones, las formas con los manifiestos. Asímismo, uno de los principales intereses es explorar los conceptos de modernidad y de identidad, como parte de la construcción misma de la arquitectura de dicha época y del concepto de “lo mexicano”. A pesar del énfasis que se ha dado en la construcción de un canon, muchas veces ligado a la noción de monumentalidad, regionalismo, y mestizaje, este trabajo parte de una mirada puesta no en las formas sino en los procesos. A partir de las conexiones entre distintas capas de información, se buscan nuevas maneras de abordar el proyecto arquitectónico. El crítico de arquitectura brasileño Hugo Segawa ha descrito la investigación sobre la arquitectura latinoamericana como “una tarea más de índole arqueológica que historiográfica”, sin embargo, también ha calificado a México como “el más vigoroso centro de debates teóricos en Latinoamérica a lo largo del siglo XX.” Ese descompas entre la ruina y el vigor, entre la abundancia de producción y la precariedad de su conservación, ha definido no solo el estudio de la arquitectura sino las propias formas de creación. Por tanto, la tesis se plantea como una nueva plataforma desde la cual sea posible reformular la arquitectura, lejos de su condición amnésica, pensada en cambio, como un sistema basado en una misma voluntad por indagar y crear. Se busca, siguiendo al crítico británico Anthony Vidler, “relacionar” la historia con el proyecto. Con el fin de quitarle lo escurridizo a una historia incompleta y sobre todo de poder entender la manera en que las ideas se convierten en forma o en objeto, la tesis se estructura a partir de 22 líneas de tiempo organizadas en tres recorridos que se cruzan: arquitectura; arte y pensamiento. A partir de referencias como el Atlas Mnemosyne de Aby Wargurg o la serie Asterisms del artista Gabriel Orozco, se crean nuevos dispositivos para ver. De tal manera, se desdoblan los distintos temas para trazar relaciones entre la ciudad, los edificios, las utopías, las publicaciones y la publicidad. El trabajo se construye como un nuevo instrumento de exploración articulado por medio de capas, como un mapa genealógico evolutivo. El objetivo es abarcar aquella arquitectura construida no sólo en la ciudad sino también en el papel. Iniciando con el trabajo de la generación que llevó la arquitectura al siglo XX, el estudio se extiende a manera de epílogo hasta la primera década del siglo XXI, reuniendo obras que normalmente se han visto de manera aislada para entenderlas en su contexto más amplio. Como escenario de búsquedas, esta tesis intenta provocar el cruce de significados, creyendo imprescindible una nueva reflexión en torno a la disciplina y a los escenarios en los cuales se inscribe. La arquitectura de México –un país que en el siglo XX pasó de tener 13 millones de habitantes a 100 millonescorresponde esencialmente a una producción anónima, o bien, fabricada a partir de estereotipos. Pero entre la mancha de desarrollo informal y el hito reconocible está un trabajo tan amplio como inexplorado. Por tanto, se ofrece una serie de nuevas constelaciones que comprenden desde la Revolución de 1910 a los Juegos Olímpicos de 1968; del terremoto de la ciudad de México en 1985 a los concursos internacionales de las últimas décadas. This thesis’ hypothesis states that architectural modernity in Mexico is not, as sometimes pretended, a homogeneous history, focused on some key figures, but rather a multiple and complex narrative, in which art and print media have played an essential role. Therefore, it proposes a new perspective on 20th century architecture in Mexico analized through the relationship between architecture and photography, art, theory and media. Its aim is to link architecture and artistic movements, authors and publications, forms and manifestos. What is intended here is to explore the concepts of ‘modernity’ and ‘identity’ as part of the construction of architecture and the concept of ‘Mexicanity’. Despite the emphasis that has been given to the construction of an architectural canon —mostly related to the notions of monumentality, regionalism and mestizaje/métissage— this thesis’ approach is focused mainly in processes and not in forms. Through connections between diverse layers of information, new ways of dealing with the architectural project are explored. Brazilian architecture critic Hugo Segawa has described the research on Latin American architecture as «more a task of archaeology than of historiography». Nonetheless, he has also described Mexico as «the most vigorous center of theoretical debates in Latin America throughout the 20th century». This acute discrepancy between decay and vigor, between abundance of production and precarious state of conservation has determined not only the ways in which architecture is studied and understood but also the process of architectural creation. This work is therefore outlined as a new platform in order to reformulate the discipline as a system based on a common will to research and create, far from the existing amnesiac attitude. Following British critic Anthony Vidler, the interest relies in the attempt to ‘relate’ History to project. In order to reduce the elusiveness of an incomplete history and, specially, to understand how ideas become forms and objects, this thesis is composed of 22 timelines organized in three intersecting itineraries: Architecture, Art and Theory. Drawing inspiration from Aby Warburg’s Atlas Mnemosyne and Gabriel Orozco’s series Asterisms, new exploration devices are created. In such a way, diverse topics unfold to draw connections between built environment, utopian projects, publications, photography and publicity. This work is developed as a new tool for exploration, articulated by layers, like an evolutionary genealogy map. Its objective is to analyze not only the architecture build in cities, but produced on paper. Starting with the work of the generation that led Mexican architecture into the 20th century, this research extends until the first decade of the 21st century (the epilogue), gathering together works which have been usually seen in isolation, and therefore making possible its understanding in a broader context. As a scenario for exploration, this work tries to prompt the crossing of meanings, in the belief that new approaches on the discipline and its context are needed. Architecture in Mexico — a country whose population grew in the 20th century form 13 to 100 million— is related essentially with an anonymous production, or else made from stereotypes. However, between the sprawl of informal urban developments and landmark buildings there is an architectural production as extensive as it is unexamined. This essay introduces a series of new constellations, ranging from the Revolution in 1910 to the Olympic Games in 1968; from the earthquake in Mexico City in 1985 to the international competitions of the last decade. These myriad perspectives present buildings that were never built, forgotten writings, iconic images and unpublished material.

Estudio y desarrollo de sensores de fibra óptica para detección de vibraciones en ductos ascendentes submarinos

En esta tesis doctoral se describe el trabajo de investigación enfocado al estudio y desarrollo de sensores de fibra óptica para la detección de presión, flujo y vibraciones en ductos ascendentes submarinos utilizados en la extracción y transporte de hidrocarburos, con el objetivo de aplicarlos en los campos de explotación de aguas profundas en el Golfo de México pertenecientes a la Industria Petrolera Mexicana. El trabajo se ha enfocado al estudio y desarrollo de sensores ópticos cuasi distribuidos y distribuidos. En especial se ha profundizado en el uso y aplicación de las redes de Bragg (FBG) y de reflectómetros ópticos en el dominio del tiempo sensible a la fase (φ-OTDR). Los sensores de fibra óptica son especialmente interesantes para estas aplicaciones por sus ventajosas características como su inmunidad a interferencias electromagnéticas, capacidad de multiplexado, fiabilidad para trabajar en ambientes hostiles, altas temperaturas, altas presiones, ambientes salino-corrosivos, etc. Además, la fibra óptica no solo es un medio sensor sino que puede usarse como medio de transmisión. Se ha realizado un estudio del estado del arte y las ventajas que presentan los sensores ópticos puntuales, cuasi-distribuidos y distribuidos con respecto a los sensores convencionales. Se han estudiado y descrito los interrogadores de redes de Bragg y se ha desarrollado un método de calibración útil para los interrogadores existentes en el mercado, consiguiendo incertidumbres en la medida de la longitud de onda menores de ± 88 nm e incertidumbres relativas (la mas interesante en el campo de los sensores) menores de ±3 pm. Centrándose en la aplicación de las redes de Bragg en la industria del petróleo, se ha realizado un estudio en detalle del comportamiento que presentan las FBGs en un amplio rango de temperaturas de -40 ºC a 500 oC. Como resultado de este estudio se han evaluado las diferencias en los coeficientes de temperatura en diversos tramos de mas mismas, así como para diferentes recubrimientos protectores. En especial se ha encontrado y evaluado las diferencias de los diferentes recubrimientos en el intervalo de temperaturas entre -40 ºC y 60 ºC. En el caso del intervalo de altas temperaturas, entre 100 ºC y 500 ºC, se ha medido y comprobado el cambio uniforme del coeficiente de temperatura en 1pm/ºC por cada 100 ºC de aumento de temperatura, en redes independientemente del fabricante de las mismas. Se ha aplicado las FBG a la medición de manera no intrusiva de la presión interna en una tubería y a la medición del caudal de un fluido en una tubería, por la medida de diferencia de presión entre dos puntos de la misma. Además se ha realizado un estudio de detección de vibraciones en tuberías con fluidos. Finalmente, se ha implementado un sistema de detección distribuida de vibraciones aplicable a la detección de intrusos en las proximidades de los ductos, mediante un φ-OTDR. En este sistema se ha estudiado el efecto negativo de la inestabilidad de modulación que limita la detección de vibraciones distribuidas, su sensibilidad y su alcance. ABSTRACT This thesis describes the research work focused for the study and development of on optical fiber sensors for detecting pressure, flow and vibration in subsea pipes used in the extraction and transportation of hydrocarbons, in order to apply them in deepwater fields in the Gulf of Mexico belonging to the Mexican oil industry. The work has focused on the study and development of optical sensors distributed and quasi distributed. Especially was done on the use and application of fiber Bragg grating (FBG) and optical reflectometers time domain phase sensitive (φ-OTDR). The optical fiber sensors especially are interesting for these applications for their advantageous characteristics such as immunity to electromagnetic interference, multiplexing capability, reliability to work in harsh environments, high temperatures, high pressures, corrosive saline environments, etc. Furthermore, the optical fiber is not only a sensor means it can be used as transmission medium. We have performed a study of the state of the art and the advantages offered by optical sensors point, quasi-distributed and distributed over conventional sensors. Have studied and described interrogators Bragg grating and has developed a calibration method for interrogators useful for the existing interrogators in the market, resulting uncertainty in the measurement of the wavelength of less than ± 0.17 nm and uncertainties (the more interesting in the field of sensors) less than ± 3 pm. Focusing on the application of the Bragg gratings in the oil industry, has been studied in detail the behavior of the FBGs in a wide range of temperatures from -40 °C to 500 oC. As a result of this study we have evaluated the difference in temperature coefficients over various sections of the same, as well as different protective coatings. In particular evaluated and found the differences coatings in the range of temperatures between -40 º C and 60 º C. For the high temperature range between 20 ° C and 500 ° C, has been measured and verified the uniform change of the temperature coefficient at 1pm / ° C for each 100 ° C increase in temperature, in networks regardless of manufacturer thereof. FBG is applied to the non-intrusive measurement of internal pressure in a pipeline and measuring flow of a fluid in a pipe, by measuring the pressure difference between two points thereof. Therefore, has also made a study of detecting vibrations in pipes with fluids. Finally, we have implemented a distributed sensing system vibration applied to intrusion detection in the vicinity of the pipelines, by φ-OTDR. In this system we have studied the negative effect of modulation instability limits the distributed vibration detection, sensitivity and scope.

A Focused Crawler in order to Get Semantic Web Resources (CSR)

This paper presents a Focused Crawler in order to Get Semantic Web Resources (CSR). Structured data web are available in formats such as Extensible Markup Language (XML), Resource Description Framework (RDF) and Ontology Web Language (OWL) that can be used for processing. One of the main challenges for performing a manual search and download semantic web resources is that this task consumes a lot of time. Our research work propose a focused crawler which allow to download these resources automatically and store them on disk in order to have a collection that will be used for data processing. CRS consists of three layers: (a) The User Interface Layer, (b) The Focus Crawler Layer and (c) The Base Crawler Layer. CSR uses as a selection policie the Shark-Search method. CSR was conducted with two experiments. The first one starts on December 15 2012 at 7:11 am and ends on December 16 2012 at 4:01 were obtained 448,123,537 bytes of data. The CSR ends by itself after to analyze 80,4375 seeds with an unlimited depth. CSR got 16,576 semantic resources files where the 89 % was RDF, the 10 % was XML and the 1% was OWL. The second one was based on the Web Data Commons work of the Research Group Data and Web Science at the University of Mannheim and the Institute AIFB at the Karlsruhe Institute of Technology. This began at 4:46 am of June 2 2013 and 1:37 am June 9 2013. After 162.51 hours of execution the result was 285,279 semantic resources where predominated the XML resources with 99 % and OWL and RDF with 1 % each one.

Modelos de asociación público-privada en la provisión de infraestructura: la experiencia internacional y el desarrollo de las autopistas en México

Análisis de los modelos de asociación público-privado utilizados en la construcción de autopistas en México en los últimos 25 años. Existen estudios que respaldan la opinión de que los niveles de competitividad están estrechamente relacionados con el desarrollo de la infraestructura de los países. La baja competitividad de México debido a la mala calidad de su infraestructura, pone en evidencia la necesidad del mejoramiento y provisión de la misma en un marco de escasez de recursos fiscales. Esta argumentación, sustenta la intervención del sector privado para apoyar el financiamiento de infraestructura mediante nuevos mecanismos de asociación público privada (APP). En el artículo se describen: el desarrollo de la infraestructura carretera de México, la forma en que ésta fue financiada en el pasado con modelos de concesión para la construcción de carreteras de alta calidad y los modelos de APP que el país está utilizando para atraer la inversión privada en el actual programa mexicano de autopistas. Se muestran los beneficios del empleo de estos esquemas de financiamiento y se ofrece una serie de recomendaciones para transparentar el uso de estos modelos. There are studies that support the view that the competitiveness levels are closely related to the development of the country’s infrastructure. The low competitiveness of Mexico due to the poor quality of its infrastructure, underscores the need for improvement and provision of the same in a context of scarce fiscal resources. This argument supports the intervention of the private sector to finance infrastructure through new mechanisms for public private partnership (PPP). The article describes the development of the Mexico road infrastructure, the way in which it was funded in the past by models of concession for the construction of high-quality roads and models of app that the country is using to attract private investment in the current Mexican highways program. The benefits of these funding schemes are displayed and recommendations are offered for transparent use of these models

Mecanismos implicados en las etapas iniciales de infección en la cancrosis de los cítricos provocada por Xanthomonas citri subsp. citri

La cancrosis o chancro bacteriano de los cítricos (CBC) causada por Xanthomonas citri subsp. citri (Xcc) y X. fuscans subsp. aurantifolii, afecta a un gran número de especies dentro de la familia de las rutáceas, especialmente cítricos. Esta enfermedad produce graves pérdidas económicas allí donde está presente, principalmente porque la comercialización de cítricos desde las zonas afectadas hacía zonas libres de cancrosis, está sujeta a fuertes medidas cuarentenarias. La cancrosis se encuentra distribuida a nivel mundial pero no se ha localizado ni en la Unión Europea ni en ningún área del Mediterráneo. Se han descrito tres tipos de cancrosis en función de la gama de huésped y de las características fenotípicas y genotípicas de las bacterias que las producen. La más extendida es la cancrosis tipo A producida por Xcc, dentro de la cual se distinguen los subtipos Aw y A*, originarios de Florida y Sudeste Asiático, respectivamente, que de forma natural solo son capaces de producir enfermedad en lima mejicana. En este trabajo se presentan estudios sobre mecanismos implicados en las primeras etapas de la infección, como la quimiotaxis y formación de biopelículas, en la cancrosis de los cítricos. La quimiotaxis es el proceso por el cual las bacterias se dirigen hacia zonas favorables para su supervivencia y desarrollo. Los perfiles quimiotácticos obtenidos frente a distintas fuentes de carbono, así como los estudios en relación al contenido de proteínas aceptoras de grupos metilo (MCPs), permitieron agrupar a las cepas de Xanthomonas estudiadas en este trabajo, de acuerdo a la enfermedad producida y a su gama de huésped. Todas las cepas mostraron quimiotaxis positiva frente a extractos de hoja y apoplasto de diferentes especies, sin embargo, Xcc 306, X. alfalfae subsp. citrumelonis (Xac) y X. campestris pv. campestris (Xc) manifestaron respuestas más específicas frente a extractos de apoplasto de hojas de naranjo dulce, lima y col china, respectivamente. Dicho resultado nos permite asociar el mecanismo de quimiotaxis con la capacidad de las cepas de Xanthomonas para colonizar estos huéspedes de forma específica. Las cepas estudiadas fueron capaces de realizar movimiento tipo swimming, twitching y sliding en distintos medios, siendo el movimiento swimming el único en el que se encontraron diferencias entre las cepas de Xcc con distinta gama de huésped. En este trabajo se ha estudiado además la formación de biopelículas en superficies bióticas y abióticas, un mecanismo importante tanto para la supervivencia en superficie vegetal como para el desarrollo de la infección. Las cepas de Xanthomonas estudiadas fueron capaces de formar biopelículas in vitro, siendo mayor en un medio que simula el apoplasto y que contiene una baja concentración de nutrientes en comparación con medios que contenían alta concentración de nutrientes. La formación de biopelículas en superficie vegetal se encontró relacionada, en las cepas patógenas de cítricos, con la capacidad para infectar un tejido o huésped determinado. Se han caracterizado algunos de los componentes de la matriz extracelular producida por Xcc, que compone hasta un 90% de las bipoelículas. Entre ellos destaca el ADN extracelular, que tiene un papel como adhesina en las primeras etapas de formación de biopelículas y estructural en biopelículas maduras. Además, se han identificado el pilus tipo IV como componente importante en las biopelículas, que también participa en motilidad. Finalmente, se han realizado estudios sobre la expresión de genes implicados en motilidad bacteriana y formación de biopelículas que han confirmado las diferencias existentes entre cepas de Xcc de amplia y limitada gama de huésped, así como el papel que juegan elementos como el pilus tipo IV o el flagelo en estos procesos. ABSTRACT Xanthomonas citri subsp. citri (Xcc) and X. fuscans subsp. aurantifolii are the causal agents of Citrus Bacterial Canker (CBC) which is one of the most important citrus diseases. CBC affects all Citrus species as well as other species from Rutaceae family. CBC produces strong economic losses; furthermore the commercialization of plants and fruits is restricted from infested to citrus canker free areas. The disease is worldwide distributed in tropical and subtropical areas, however it is not present in the European Union. Three types of CBC have been described according to the host range and phenotypic and genotypic characteristics. CBC type A caused by Xcc is he widest distributed. Within CBC A type two subtypes Aw and A* were described from Florida and Iran respectively, both infecting only Mexican lime. Herein mechanisms connected to early events in the citrus bacterial canker disease such as chemotaxis and biofilm formation, were studied. Chemotaxis allows bacteria to move towards the more suitable environments for its survival, host colonization and infection. Studies performed on citrus pathogenic Xanthomonas and X. campestris pv. campestris (Xc), a crucifer pathogen, have shown different chemotactic profiles towards carbon compound as well as different MCPs profile, which clustered strains according to host range and disease caused. Every strain showed positive chemotaxis toward leaf extracts and apoplastic fluids from sweet orange, Mexican lime and Chinese cabbage leaves. However, a more specific response was found for strains Xcc 306, X. alfalfae subsp. citrumelonis and Xc towards sweet orange, Mexican lime and Chinese cabbage apoplastic fluids, respectively. These results relate chemotaxis with the higher ability of those strains to specifically colonize their proper host. Xanthomonas strains studied were able to perform swimming, sliding and twitching motilities. The ability to swim was variable among CBC strains and seemed related to host range. Biofilm formation is an important virulence factor for Xcc because it allows a better survival onto the plant surface as well as facilitates the infection process. The studied Xanthomonas strains were able to form biofilm in vitro, on both nutrient rich and apoplast mimicking media, furthermore the biofilm formation by all the strains was higher in the apoplast mimicking media. The ability to form biofilm in planta by Xcc and Xac strains was dependent of the host and the tissue colonized. The wide host range CBC strain was able to form biofilm onto several citrus leaves and fruits, however the limited host range CBC strain produced biofilm solely onto Mexican lime leaves and fruits. Furthermore Xac strain, which solely infects leaves of young plants, was not able to develop biofilms on fruits. Some components of the extracellular matrix produced by Xcc strains have been characterized. Extracellular DNA acted as an adhesin at the very early stages of biofilm formation and as structural component of mature biofilm for citrus pathogenic Xanthomonas. Furthermore type IV pilus has been identified as a component of the extracellular matrix in biofilm and motility. Transcriptional studies of genes related with biofilm formation and motility have confirmed the differential behavior found among wide and limited host range CBC strains as well as the role of type IV pili and flagellum on those processes.

Evaluación de la distribución de riesgos en los distintos modelos de asociación público-privada para la provisión de infraestructura carretera en México

México es de los pocos países en el mundo que ha realizado dos grandes programas para la construcción de autopistas en colaboración con el sector privado. El primero, fue realizado entre 1989 y 1994, con resultados adversos por el mal diseño del esquema de concesiones; y, el segundo con mejores resultados, en operación desde 2003 mediante nuevos modelos de asociación público-privada (APP). El objetivo de la presente investigación es estudiar los modelos de asociación público-privada empleados en México para la provisión de infraestructura carretera, realizando el análisis y la evaluación de la distribución de riesgos entre el sector público y privado en cada uno de los modelos con el propósito de establecer una propuesta de reasignación de riesgos para disminuir el costo global y la incertidumbre de los proyectos. En la primera parte se describe el estado actual del conocimiento de las asociaciones público-privadas para desarrollar proyectos de infraestructura, incluyendo los antecedentes, la definición y las tipologías de los esquemas APP, así como la práctica internacional de programas como el modelo británico Private Finance Initiative (PFI), resultados de proyectos en la Unión Europea y programas APP en otros países. También, se destaca la participación del sector privado en el financiamiento de la infraestructura del transporte de México en la década de 1990. En los capítulos centrales se aborda el estudio de los modelos APP que se han utilizado en el país en la construcción de la red de carreteras de alta capacidad. Se presentan las características y los resultados del programa de autopistas 1989-94, así como el rescate financiero y las medidas de reestructuración de los proyectos concesionados, aspectos que obligaron a las autoridades mexicanas a cambiar la normatividad para la aprobación de los proyectos según su rentabilidad, modificar la legislación de caminos y diseñar nuevos esquemas de colaboración entre el gobierno y el sector privado. Los nuevos modelos APP vigentes desde 2003 son: nuevo modelo de concesiones para desarrollar autopistas de peaje, modelo de proyectos de prestación de servicios (peaje sombra) para modernizar carreteras existentes y modelo de aprovechamiento de activos para concesionar autopistas de peaje en operación a cambio de un pago. De estos modelos se realizaron estudios de caso en los que se determinan medidas de desempeño operativo (niveles de tráfico, costos y plazos de construcción) y rentabilidad financiera (tasa interna de retorno y valor presente neto). En la última parte se efectúa la identificación, análisis y evaluación de los riesgos que afectaron los costos, el tiempo de ejecución y la rentabilidad de los proyectos de ambos programas. Entre los factores de riesgo analizados se encontró que los más importantes fueron: las condiciones macroeconómicas del país (inflación, producto interno bruto, tipo de cambio y tasa de interés), deficiencias en la planificación de los proyectos (diseño, derecho de vía, tarifas, permisos y estimación del tránsito) y aportaciones públicas en forma de obra. Mexico is one of the few countries in the world that has developed two major programs for highway construction in collaboration with the private sector. The first one was carried out between 1989 and 1994 with adverse outcomes due to the wrong design of concession schemes; and, the second one, in operation since 2003, through new public-private partnership models (PPPs). The objective of this research is to study public-private partnership models used in Mexico for road infrastructure provision, performing the analysis and evaluation of risk’s distribution between the public and the private sector in each model in order to draw up a proposal for risk’s allocation to reduce the total cost and the uncertainty of projects. The first part describes the current state of knowledge in public-private partnership to develop infrastructure projects, including the history, definition and types of PPP models, as well as international practice of programs such as the British Private Finance Initiative (PFI) model, results in the European Union and PPP programs in other countries. Also, it stands out the private sector participation in financing of Mexico’s transport infrastructure in 1990s. The next chapters present the study of public-private partnerships models that have been used in the country in the construction of the high capacity road network. Characteristics and outcomes of the highway program 1989-94 are presented, as well as the financial bailout and restructuring measures of the concession projects, aspects that forced the Mexican authorities to change projects regulations, improve road’s legislation and design new schemes of cooperation between the Government and the private sector. The new PPP models since 2003 are: concession model to develop toll highways, private service contracts model (shadow toll) to modernize existing roads and highway assets model for the concession of toll roads in operation in exchange for a payment. These models were analyzed using case studies in which measures of operational performance (levels of traffic, costs and construction schedules) and financial profitability (internal rate of return and net present value) are determined. In the last part, the analysis and assessment of risks that affect costs, execution time and profitability of the projects are carried out, for both programs. Among the risk factors analyzed, the following ones were found to be the most important: country macroeconomic conditions (inflation, gross domestic product, exchange rate and interest rate), deficiencies in projects planning (design, right of way, tolls, permits and traffic estimation) and public contributions in the form of construction works.

El sistema de escuelas primarias de Juan O'Gorman, arquitecto : modernidad y eficiencia

El proceso de proyecto, llevado a cabo por Juan O’Gorman, para proyectar y construir más de veinticinco Escuelas Primarias en el Distrito Federal en menos de seis meses en 1932, necesariamente debió regirse por una metodología que permitiera conseguirlo. Esta investigación plantea la existencia de un método de proyecto que engloba toda la arquitectura de la etapa funcionalista de O’Gorman. Se considera la metodología, denominada en esta tesis Sistema Modular de las Escuelas Primarias, como un procedimiento proyectual evolutivo, conseguido a través de una investigación y experimentación continua. Inicia en 1929 con la realización de dos proyectos de vivienda particular, continúa con la génesis de un esquema de modulación que surge en unos proyectos de vivienda colectiva, se perfecciona en los proyectos de Escuelas Primarias del D.F. introduciendo criterios de estandarización y culmina con la adaptación a diversas circunstancias geométricas y climáticas que define la ubicación, en los casos concretos de la Escuela Vocacional Industrial de Tolsá y Tresguerras y la Escuela Primaria de Tampico en 1934. Este planteamiento destaca el valor de la obra funcionalista de aquel joven arquitecto, no sólo por lo novedoso que este nuevo lenguaje supuso en el México neo-colonial de los años 30, sino especialmente por la importancia que significó para la arquitectura mexicana contemporánea, la sistematización de procesos de proyecto -modulación y estandarización- para abordar una arquitectura inexistente en la época -la arquitectura social- de manera moderna y eficiente. La metodología empleada en la tesis implica la catalogación y mapeado de las Escuelas Primarias del D.F., lo cual aporta documentación original inédita. ABSTRACT The project process, carried out by Juan O’Gorman, to design and build more than twenty five elementary schools in less than six months in Mexico City in 1932, must have necessarily been governed by a thorough methodology in order to achieve this. This research posits the existence of a project method that encompasses all the architecture of O’Gorman’s functionalist stage. The methodology, known as “Modular System of Elementary Schools” in this thesis, is regarded as an evolutionary proyectual process, achieved through continuous research and experimentation. This methodology, which started in 1929 with the completion of two projects of private dwellings, continued with the creation of a modulation scheme arising from collective housing projects, later perfected in the Elementary School projects of Mexico City by introducing standardization criteria. Finally, it culminated in the adaptation to various geometric and climatic circumstances defined by the location, in the specific cases of the Industrial Vocational School of Tolsá and Tresguerras and Tampico’s Elementary School in 1934. This approach highlights the value of the young architect’s functionalist work, not only for the novelty of this new language in the neo-colonial Mexico of the 1930s, but especially because of the importance it meant to contemporary Mexican architecture, the systematization of project processes, -modulation and standardization- and addressing a nonexistent ‘social architecture’ at the time in a modern and efficient way. The methodology used in this thesis involves cataloging and mapping O’Gorman’s Elementary Schools in Mexico City, which provides unprecedented original documentation.

A INFLUÊNCIA DAS PRÁTICAS PEDAGÓGICAS DOCENTES E DAS BARREIRAS DISCENTES SOBRE O DESENVOLVIMENTO DA CRIATIVIDADE DO FUTURO ADMINISTRADOR

As empresas que almejam garantir e melhorar sua posição dentro de em um mercado cada vez mais competitivo precisam estar sempre atualizadas e em constante evolução. Na busca contínua por essa evolução, investem em projetos de Pesquisa & Desenvolvimento (P&D) e em seu capital humano para promover a criatividade e a inovação organizacional. As pessoas têm papel fundamental no desenvolvimento da inovação, mas para que isso possa florescer de forma constante é preciso comprometimento e criatividade para a geração de ideias. Criatividade é pensar o novo; inovação é fazer acontecer. Porém, encontrar pessoas com essas qualidades nem sempre é tarefa fácil e muitas vezes é preciso estimular essas habilidades e características para que se tornem efetivamente criativas. Os cursos de graduação podem ser uma importante ferramenta para trabalhar esses aspectos, características e habilidades, usando métodos e práticas de ensino que auxiliem no desenvolvimento da criatividade, pois o ambiente ensino-aprendizagem pesa significativamente na formação das pessoas. O objetivo deste estudo é de identificar quais fatores têm maior influência sobre o desenvolvimento da criatividade em um curso de graduação em administração, analisando a influência das práticas pedagógicas dos docentes e as barreiras internas dos discentes. O referencial teórico se baseia principalmente nos trabalhos de Alencar, Fleith, Torrance e Wechsler. A pesquisa transversal de abordagem quantitativa teve como público-alvo os alunos do curso de Administração de uma universidade confessional da Grande São Paulo, que responderam 465 questionários compostos de três escalas. Para as práticas docentes foi adaptada a escala de Práticas Docentes em relação à Criatividade. Para as barreiras internas foi adaptada a escala de Barreiras da Criatividade Pessoal. Para a análise da percepção do desenvolvimento da criatividade foi construída e validada uma escala baseada no referencial de características de uma pessoa criativa. As análises estatísticas descritivas e fatoriais exploratórias foram realizadas no software Statistical Package for the Social Sciences (SPSS), enquanto as análises fatoriais confirmatórias e a mensuração da influência das práticas pedagógicas e das barreiras internas sobre a percepção do desenvolvimento da criatividade foram realizadas por modelagem de equação estrutural utilizando o algoritmo Partial Least Squares (PLS), no software Smart PLS 2.0. Os resultados apontaram que as práticas pedagógicas e as barreiras internas dos discentes explicam 40% da percepção de desenvolvimento da criatividade, sendo as práticas pedagógicas que exercem maior influencia. A pesquisa também apontou que o tipo de temática e o período em que o aluno está cursando não têm influência sobre nenhum dos três construtos, somente o professor influencia as práticas pedagógicas.

A mutation in the transmembrane/luminal domain of the ryanodine receptor is associated with abnormal Ca2+ release channel function and severe central core disease

Central core disease is a rare, nonprogressive myopathy that is characterized by hypotonia and proximal muscle weakness. In a large Mexican kindred with an unusually severe and highly penetrant form of the disorder, DNA sequencing identified an I4898T mutation in the C-terminal transmembrane/luminal region of the RyR1 protein that constitutes the skeletal muscle ryanodine receptor. All previously reported RYR1 mutations are located either in the cytoplasmic N terminus or in a central cytoplasmic region of the 5,038-aa protein. The I4898T mutation was introduced into a rabbit RYR1 cDNA and expressed in HEK-293 cells. The response of the mutant RyR1 Ca2+ channel to the agonists halothane and caffeine in a Ca2+ photometry assay was completely abolished. Coexpression of normal and mutant RYR1 cDNAs in a 1:1 ratio, however, produced RyR1 channels with normal halothane and caffeine sensitivities, but maximal levels of Ca2+ release were reduced by 67%. [3H]Ryanodine binding indicated that the heterozygous channel is activated by Ca2+ concentrations 4-fold lower than normal. Single-cell analysis of cotransfected cells showed a significantly increased resting cytoplasmic Ca2+ level and a significantly reduced luminal Ca2+ level. These data are indicative of a leaky channel, possibly caused by a reduction in the Ca2+ concentration required for channel activation. Comparison with two other coexpressed mutant/normal channels suggests that the I4898T mutation produces one of the most abnormal RyR1 channels yet investigated, and this level of abnormality is reflected in the severe and penetrant phenotype of affected central core disease individuals.

The role of Wolbachia bacteria in reproductive incompatibilities and hybrid zones of Diabrotica beetles and Gryllus crickets

A rickettsial bacterium in the genus Wolbachia is the cause of a unidirectional reproductive incompatibility observed between two major beetle pests of maize, the western corn rootworm, Diabrotica virgifera virgifera, and the Mexican corn rootworm, D. v. zeae. These subspecies are allopatric except for two known regions of sympatry in Texas and Mexico. We demonstrate that populations of D. v. virgifera, with the exception of two populations in southern Arizona, are infected with a strain of Wolbachia. Populations of D. v. zeae are not infected. Treatment of D. v. virgifera with tetracycline eliminated the Wolbachia and removed the reproductive incompatibility. Similar patterns of reproductive incompatibility exist among taxa of the cricket genus Gryllus. Gryllus assimilis, G. integer, G. ovisopis, G. pennsylvanicus, and G. rubens are infected with Wolbachia whereas G. firmus is usually not. Populations of G. rubens and G. ovisopis carry the same Wolbachia strain, which is distinct from that of G. integer. G. pennsylvanicus is infected with two Wolbachia strains, that found in G. rubens and one unique to G. pennsylvanicus. Moreover, a proportion of G. pennsylvanicus individuals harbors both strains. Wolbachia may have influenced speciation in some members of the genus Gryllus by affecting the degree of hybridization between species. Given that Wolbachia infections are relatively common in insects, it is likely that other insect hybrid zones may be influenced by infections with Wolbachia.

The role of thyroid hormone in zebrafish and axolotl development

Exogenous thyroid hormone (TH) induces premature differentiation of the zebrafish pectoral fins, which are analogous to the forelimbs of tetrapods. It accelerates the growth of the pelvic fins but not precociously. Goitrogens, which are chemical inhibitors of TH synthesis by the thyroid gland, inhibit the transition from larva to juvenile fish including the formation of scales, and pigment pattern; they stunt the growth of both pectoral and pelvic paired fins. Inhibition by goitrogens is rescued by the simultaneous addition of thyroxine. The effect of adding TH to the rearing water of the postembryonic Mexican axolotl was reinvestigated under conditions that permit continued growth and development. In addition to morphological changes that have been described, TH greatly stimulates axolotl limb growth causing the resulting larva to be proportioned as an adult in about two months. This study extends the known evolutionary relatedness of tetrapod limbs and fish fins to include the TH stimulation of salamander limb and zebrafish fin growth, and suggests that TH is required to complete the life cycle of a typical bony fish and a salamander at the same developmental stage that it controls anuran and flounder metamorphosis.

La filosofía marxista entre Francia y América Latina : Una lectura de la correspondencia entre Louis Althusser y Fernanda Navarro

En 1988, fue publicado por Siglo XXI México Filosofía y marxismo, un pequeño libro de entrevistas al filósofo marxista francés Louis Althusser realizadas por la profesora de filosofía mexicana Fernanda Navarro. En dicho volumen, Althusser retomaba varios de los aspectos fundamentales que habían caracterizado su intervención durante las décadas de 1960 y 1970 e introducía elementos absolutamente novedosos como las formulaciones alrededor del materialismo aleatorio y la filosofía del encuentro. La correspondencia mantenida entre Althusser y Navarro entre la entrevista de 1984 y la publicación de Filosofía y marxismo en 1988, habilita un horizonte interpretativo que permite integrar el vínculo establecido entre ambos filósofos en el marco más general del itinerario teórico-político de Althusser en Francia y de las proyecciones del althusserianismo hacia América Latina. Veremos de qué manera aquel encuentro entre Althusser y Navarro estuvo originado en un intercambio epistolar previo entre el filósofo francés y Mauricio Malamud, un comunista argentino difusor de la obra de Althusser en Argentina y por entonces exiliado en Morelia. Asimismo, la correspondencia entre Althusser y Navarro nos permite ver que el hecho de que el libro haya sido publicado únicamente en América Latina fue resultado de un interés específico de Althusser en los procesos políticos latinoamericanos. Finalmente, el intercambio permite captar el tenor filosófico del mencionado libro en el marco más general de la especificidad de la intervención althusseriana