E-Ciencias de la Información: An electronic journal serving the transfer of knowledge

The article introduces the concept of E-Science and its importance in the generation of knowledge. This article also stresses the need to transfer that knowledge, before this, the School of Library and Information Science at the University of Costa Rica could gothrough such means as the online magazine E-Ciencias de la Información.It explains why we chose the form of electronic publication and describes the development strategy, architecture design and technological requirements. The article concludes that electronic publications have penetrated hard in the academic world and are increasingly cited, so that their contribution as vehicles for dissemination of knowledge is undeniable.

Large-scale screening of a targeted Enterococcus faecalis mutant library identifies envelope fitness factors

Spread of antibiotic resistance among bacteria responsible for nosocomial and community-acquired infections urges for novel therapeutic or prophylactic targets and for innovative pathogen-specific antibacterial compounds. Major challenges are posed by opportunistic pathogens belonging to the low GC% gram-positive bacteria. Among those, Enterococcus faecalis is a leading cause of hospital-acquired infections associated with life-threatening issues and increased hospital costs. To better understand the molecular properties of enterococci that may be required for virulence, and that may explain the emergence of these bacteria in nosocomial infections, we performed the first large-scale functional analysis of E. faecalis V583, the first vancomycin-resistant isolate from a human bloodstream infection. E. faecalis V583 is within the high-risk clonal complex 2 group, which comprises mostly isolates derived from hospital infections worldwide. We conducted broad-range screenings of candidate genes likely involved in host adaptation (e.g., colonization and/or virulence). For this purpose, a library was constructed of targeted insertion mutations in 177 genes encoding putative surface or stress-response factors. Individual mutants were subsequently tested for their i) resistance to oxidative stress, ii) antibiotic resistance, iii) resistance to opsonophagocytosis, iv) adherence to the human colon carcinoma Caco-2 epithelial cells and v) virulence in a surrogate insect model. Our results identified a number of factors that are involved in the interaction between enterococci and their host environments. Their predicted functions highlight the importance of cell envelope glycopolymers in E. faecalis host adaptation. This study provides a valuable genetic database for understanding the steps leading E. faecalis to opportunistic virulence.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.

Jocassee journal

This periodical presents information and news about the Jocassee Gorges.