995 resultados para Laboratorial tests


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The aim of this study was to adapt the methodology of the accelerated aging and electrical conductivity tests for determination of physiological potential in crambe seeds. Six seed lots of crambe (cv. FMS Brilhante) were subjected to determination of moisture content, germination test, first count germination, emergence, and emergence speed index. For the accelerated aging test, the traditional methodology was used with water, and with a saturated potassium chloride and sodium chloride solution in three periods of exposure (24, 48, and 72 hours) at 41 degrees C; the electrical conductivity test was performed with four pre-soaking treatments (0, 2, 4, and 8 hours) and four soaking periods (4, 8, 16, and 24 hours) at 25 degrees C. The accelerated aging test with water for 72 hours and the electrical conductivity test with 2 hours of pre-soaking and assessment after 16 hours were effective for classification of the crambe seed lots in regard to physiological quality.

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Objectives. To compare three different designs for measuring the bond strength between Y-TZP ceramic and a composite material, before and after ceramic surface treatment, evaluating the influence of the size of the adhesive interface for each design.Methods. 'Macro'tensile, microtensile, 'macro'shear, microshear, 'macro'push-out, and micropush-out tests were carried out. Two Y-TZP surface treatments were evaluated: silanization (sil) and tribochemical silica coating (30 mu m silica-modified Al2O3 particles + silanization) (TBS). Failure mode analysis of tested samples was also performed. Results. Both the surface treatment and the size of the bonded interface significantly affected the results (p = 0.00). Regardless of the type of surface treatment, the microtensile and microshear tests had higher values than their equivalent "macro" tests. However, the push-out test showed the highest values for the "macro" test. The tensile tests showed the greatest variability in results. The tribochemical silica coating method significantly increased bond strength for all tests.Significance. Different test designs can change the outcome for Y-TZP/cement interfaces, in terms of mean values and reliability (variability). The 'micro'tests expressed higher bond strengths than their equivalent 'macro'tests, with the exception of the push-out test (macro > micro). (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

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Many models for unsaturated soil have been developed in the last years, accompanying the development of experimental techniques to deal with such soils. The benchmark of the models for unsaturated soil can be assigned to the Barcelona Basic Model (BBM) now incorporated in some codes such as the CODE_BRIGHT. Most of those models were validated considering limited laboratory test results and not much validation is available considering real field problems. This paper presents modeling results of field plate load tests performed under known suction on a lateritic unsaturated soil. The required input data were taken from laboratory tests performed under suction control. The modeling nicely reproduces field tests allowing appreciating the influence of soil suction on the stress-settlement curve. In addition, wetting induced or collapse settlements were calculated from field tests and were nicely duplicated by the numerical analysis performed.

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The main objective of this work is to illustrate an application of angular active control in a sectioned airfoil using shape memory alloys. In the proposed model, one wants to establish the shape of the airfoil profile based on the determination of an angle between its two sections. This angle is obtained by the effect of the shape memory of the alloy by passing an electric current that modifies the temperature of the wire through the Joule effect, changing the shape of the alloy. This material is capable of converting thermal energy into mechanical energy and once permanently deformed, the material can return to its original shape by heating. Due to the presence of nonlinear effects, especially in the mathematical model of the alloy, this work proposes the application of a control system based on fuzzy logic. Through numerical tests, the performance of the fuzzy controller is compared with an on-off controller applied in a sectioned airfoil model.

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The insecticide imidacloprid and the herbicide sulfentrazone are two different classes of pesticides that are used for pest control in sugarcane agriculture. To evaluate the genotoxic potential of low concentrations of these two pesticides alone and in mixture, the comet assay and the micronucleus (MN) test employing fluorescence in situ hybridization (FISH) with a centromeric probe were applied in human hepatoma cell lines (HepG2), in a 24-h assay. Mutagenicity was assessed by Salmonella/microsome assay with TA98 and TA100 strains in the absence and presence of an exogenous metabolizing system (S9). The results showed significant inductions of MN in HepG2 cells by both pesticides, for all the tested concentrations. As evidenced in the comet assay, only the imidacloprid presented significant responses. When the two pesticides were associated, a significant induction of damage was observed in the HepG2 cells by the comet assay, but not by the MN test. Moreover, the MN induced by the mixtures of the pesticides appeared at lower levels than those induced by sulfentrazone and imidacloprid when tested alone. According to the FISH results, the damage induced by imidacloprid in the HepG2 cells resulted from a clastogenic action of this insecticide (76.6% of the MN did not present a centromeric signal). For the herbicide sulfentrazone and for the mixture of the pesticides, a similar frequency of MN with and without the presence of the centromeric signal (herbicide: 52.45% of the MN without centromeric signal and 47.54% of the MN with centromeric signal; mixture: 48.71% of the MN without centromeric signal and 51.42% of the MN with centromeric signal) was verified. Based on these results, it was concluded that each one of the pesticides evaluated interacts with the DNA of HepG2 cells and causes irreparable alterations in the cells. However, the combination of the pesticides showed an antagonistic effect on the cells and the damage induced was milder and not persistent in HepG2 cells. The results obtained by the Ames test did not point out significant results.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Canine Distemper is a contagious, severe and multisystemic disease caused by a virus from Morbillivírus genus. The virus is distributed worldwide and it presents a high lethality rate, affecting mainly dogs. The diagnosis is based on clinical signs associated with hematological fi ndings. The observation of Lentz bodies in erythrocytes and leukocytes is the defi nitive diagnosis for the disease. The aim of this work was to test the hypothesis that the hematological profi le in dogs positive for canine distemper differs according to blood cell type presenting Lentz bodies. For this purpose, 25 dogs positive for the disease were evaluated at the Veterinary Hospital “Luís Quintiliano de Oliveira” UNESP, Araçatuba city. The diagnosis was based on the observation of Lentz bodies in blood smears. Fromthe total, 64% of dogs presented anemia, 16% leucopenia and 12% leukocytosis. Lymphopenia occurred in 76% of dogs. Viral inclusions were observed solely in neutrophils (32%), lymphocytes (28%) and erythrocytes (12%). Concomitant observation occurred in lymphocytes and erythrocytes (4 %), in lymphocytes and neutrophils (12%), in neutrophils and monocytes (4%) and in neutrophils and erythrocytes (4%). In an isolated case Lentz bodies were observed simultaneously in neuthrophils, monocytes and lymphocytes. In conclusion, hematological profi le is not associated with the presence of viral inclusion in a particular cell type.

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Fluoridation of water for human consumption is a method of caries prevention that brings great social benefit, if kept at optimal levels. The increase in the production of bottled water emphasizes the need to verify whether fluoride presence in these waters occurs in sufficient quantity to prevent decay, or if it represents a significant risk of fluorosis. Objective: to compare the concentrations of fluoride present in bottled water to those declared on the labels, and to make a critical analysis of legal norms on the subject. Materials and method: this was a cross-sectional study, through which 22 samples of bottled water sold in state of Ceará, Brazil, were analyzed. Analyses were performed in duplicate by the electrometric method, and results were compared to those printed on the labels. Detailed searches by laws, resolutions, ordinances, and other official documents in force, related to the topic were performed. Results: the fluoride concentrations found ranged from 0.01 to 0.36 mgF/l. Although 72.7% of the samples were classified as fluoridated, the fluoride concentrations observed were shown to be insufficient for caries prevention. As for the rules, situations where they are not clear or even divergent were found. Conclusion: the current legislation on the subject requires updates to become more objective and to create new criteria on the use of the term “fluoridated water”.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Currently has few studies today concerning the behavior of the ethyl alcohol hydrated combustible in the geological environment, results of eventual spill or leaks in a wide transport chain and distribution. This work discusses the results of an experiment in laboratorial scale, that involves the monitoring of alterations in physical properties a simulate leak of alcohol in a tank with sand saturated in water, during 4920 hours. Are applied electrical resistivity, induced polarization and spontaneous potential geophysical methods. The electric resistivity is clearly correlating to the ethanol degradation pattern. The chargeability demonstrated sensibility to this pattern, while the natural electric potential revealed direct association with the oscillations of groundwater level. The variability of the physical parameters monitoring probable control for ethanol degradation process in environment study.

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Anaerobic efforts are commonly required through repeated sprint during efforts in many sports, making the anaerobic pathway a target of training. Nevertheless, to identify improvements on such energetic way it is necessary to assess anaerobic capacity or power, which is usually complex. For this purpose, authors have postulated the use of short running performances to anaerobic ability assessment. Thus, the aim of this study was to find a relationship between running performances on anaerobic power, anaerobic capacity or repeated sprint ability. Methods Thirteen military performed maximal running of 50 (P50), 100 (P100) and 300 (P300) m on track, beyond of running-based anaerobic sprint test (RAST; RSA and anaerobic power test), maximal anaerobic running test (MART; RSA and anaerobic capacity test) and the W′ from critical power model (anaerobic capacity test). Results By RAST variables, peak and average power (absolute and relative) and maximum velocity were significantly correlated with P50 (r = −0.68, p = 0.03 and −0.76, p = 0.01; −0.83, p < 0.01 and −0.83, p < 0.01; and −0.78, p < 0.01), respectively. The maximum intensity of MART was negatively and significantly correlated with P100 (r = −0.59) and W′ was not statistically correlated with any of the performances. Conclusion MART and W′ were not correlated with short running performances, having a weak performance predicting probably due to its longer duration in relation to assessed performances. Observing RAST outcomes, we postulated that such a protocol can be used during daily training as short running performance predictor.

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This paper presents results of stress cracking tests performed in high density polyethylene (HDPE) geomembranes (GM). Stress cracking tests were performed in accordance to ASTM D5397: Notched Constant Tensile Load Test (NCTL) and Single Point-Notched Constant Tensile Load Test (SP-NCTL). Tests were conducted to the fresh sample at 50ºC (standard test) and at 70ºC (accelerated condition) in order to compare the SC values. Results from accelerated tests (NCTL) showed, for instance, a total economy of 390 hours (comparing load stages of 25% yield stress) to perform the tests.