Characterization of the Wheat Grain PKABA1-Interacting Protein TaWD40

Abscisic acid (ABA)-mediated gene expression is a critical component of plant responses to this important hormone, which affects plant growth, development, and responses to environmental stresses. Plant responses to ABA are mediated by a number of factors including PKABA1, an ABA induced protein kinase involved in ABA-suppressed gene expression in cereal grains, and TaWD40, which has previously been shown to physically interact with PKABA1. A full-length 1.9 kb TaWD40 cDNA, CK210682, was sequenced as part of this project. Based on the deduced protein sequence, it is thought that TaWD40 may belong to the family of E3 ubiquitin ligases, possibly targeting PKABA1 for destruction. Construction of expression plasmids for overproduction of the TaWD40 polypeptide in E. coli is currently underway. The TaWD40 cDNA has been successfully amplified from the source plasmid and inserted into an intermediate plasmid, pCR2.1. The TaWD40 cDNA is currently being cloned from the pCR2.1 intermediate plasmid into two different expression vectors, pRSET-A and pMAL-c2x, for future protein production and purification.

Grain refinement through controlled thermomechanical processing

The major challenge for thermomechanical processing is to extend grain refinement towards lower average grain sizes. However, there is also a need to provide a better understanding of the mechanisms through which the refinement processes proceed. Many recent proposals for advanced thermomechanical processing rely on the dynamic strain induced transformation (DSIT), and the dynamic recrystallisation of austenite as the main refinement mechanisms. These mechanisms are still not fully understood and their clarification can be expected to lead to even greater levels of refinement. The current review examines the roles of ferrite recrystallisation , DSIT and initial austenite grain size. It is shown that although the ferrite recrystallisation mechanism in DSIT has certain similarities with the well known continuous dynamic recrystallisation (CDRX), it is significantly affected by the transformed ferrite grain size. Also, reducing the initial austenite grain size increases the recrystallisation rate in the strain dependent as well as strain independent regions. These results show that the traditional concept of metadynamic recrystallisation is insufficient to explain the changes in grain size following deformation. An alternative explanation is presented.

Grain refinement of pure magnesium using rolled Zirmax® master alloy (MG-33.3ZR)

Owing to the limited solubility of zirconium in molten magnesium, almost all of the zirconium contained in the Zirmax® master alloy (Mg-33.3Zr) is present in the form of nearly pure zirconium particles. Of them, zirconium particle clusters and individual zirconium particles greater than 5

Monitoring and motivating outworkers : the case of the AMP and the sale of industrial life insurance 1905-1940

The development of labor management practices in the financial services sector provides an interesting insight into how problems associated with agency issues were overcome. Within financial institutions and other white collar occupations, the use of internal labor markets emerged as an effective means of both controlling and motivating employees. However such management techniques were only effective in cases where work tasks could be internalized. The business of some types of organizations necessitated a division of work tasks between those undertaken within the office and those undertaken outside the office. The management and sale of insurance products is a case in point. This paper explores the development of processes implemented to resolve a specific type of labor management issue, namely the control of workers under conditions of uncertainty. Using the example of the Australian Mutual Provident (Australia's largest life insurer), it analyses how and why particular work relations procedures were developed.

The curious case of the Occidental and Regal : the evolution of solvency and disclosure standards in the Australian life insurance industry

In 1990, the Australian life insurance industry was rocked by a scandal that threatened to destabilize consumer confidence in the ability of insurance providers to meet policyholder liabilities. The incident highlighted the nature of the agency problems that arise when conditions of asymmetric information exist. It revealed systemic weaknesses in accounting, solvency and disclosure standards as they applied to life insurers. This article uses an evolutionary concept of agency to analyse government and industry responses to this event. It is argued that initial adaptive responses stabilized the industry and averted a more serious crisis. Longer term innovative responses led to the introduction of a new and more rigorous approach to reporting and solvency standards, which has improved information flows and agency outcomes.