Deletion of Epstein-Barr virus latent membrane protein 1 gene in United States and Brazilian Hodgkin's disease and reactive lymphoid tissue: High frequency of a 30-bp deletion

A 30-basepair (bp) deletion in the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) gene has been reported in nasopharyngeal carcinoma and EBV-associated malignant lymphomas. Prior studies have found the deletion in about 10% to 28% of cases of Hodgkin's disease (HD), particularly in cases with aggressive histology. We studied the prevalence of 30-bp LMP1 gene deletion in EBV-positive HD in the United States (US) (12 cases) and Brazil (26 cases) with comparison to reactive lymphoid tissues (21 cases) and HD without EBV-positive Reed-Sternberg cells (15 cases). We studied the status of the LMP1 gene by Southern blot hybridization of polymerase chain reaction (PCR) products obtained after amplification with primers spanning the site of the deletion. We also performed EBV typing, EBER1 in situ hybridization, and LMP1 protein immunohistochemistry. EBV was detected in 12/26 (46%) cases of HD from the US and 26/27 (96%) cases of Brazilian HD. The 30-bp LMP1 gene deletion was observed in 4/12 (33%) cases of EBV-positive HD from US, and 12/26 (46%) cases of Brazilian EBV-positive HD, including 3 cases of type B EBV, as compared with 12/21 (57%) reactive lymphoid tissues and 9/15 (60%) cases of EBV-negative HD. US and Brazilian HD showed a higher prevalence of the 30-bp LMP1 gene deletion, compared with studies of others. The unexpected finding of high incidence of 30-bp deletion in LMP1 gene in reactive lymphoid tissue and HD without EBV-positive Reed-Sternberg cells suggests that this deletion may not be relevant to HD pathogenesis in most cases. Copyright (C) 1997 by W.B. Saunders Company.

Guatemala, censo experimental de 1970: aplicacion de las tecnicas del profesor William Brass para estimar la fecundidad y la mortalidad

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Costa Rica (Canton Grecia) 1968 y Chile 1960: algunas consideraciones del metodo del profesor William Brass para estimar fecundidad

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Continuous measurement of cerebral oxygen saturation (rSO 2) for assessment of cardiovascular status during hemorrhagic shock in a swine model

Background: Early trauma care is dependent on subjective assessments and sporadic vital sign assessments. We hypothesized that near-infrared spectroscopy-measured cerebral oxygenation (regional oxygen saturation [rSO 2]) would provide a tool to detect cardiovascular compromise during active hemorrhage. We compared rSO 2 with invasively measured mixed venous oxygen saturation (SvO2), mean arterial pressure (MAP), cardiac output, heart rate, and calculated pulse pressure. Methods: Six propofol-anesthetized instrumented swine were subjected to a fixed-rate hemorrhage until cardiovascular collapse. rSO 2 was monitored with noninvasively measured cerebral oximetry; SvO2 was measured with a fiber optic pulmonary arterial catheter. As an assessment of the time responsiveness of each variable, we recorded minutes from start of the hemorrhage for each variable achieving a 5%, 10%, 15%, and 20% change compared with baseline. Results: Mean time to cardiovascular collapse was 35 minutes ± 11 minutes (54 ± 17% total blood volume). Cerebral rSO 2 began a steady decline at an average MAP of 78 mm Hg ± 17 mm Hg, well above the expected autoregulatory threshold of cerebral blood flow. The 5%, 10%, and 15% decreases in rSO 2 during hemorrhage occurred at a similar times to SvO2, but rSO 2 lagged 6 minutes behind the equivalent percentage decreases in MAP. There was a higher correlation between rSO 2 versus MAP (R =0.72) than SvO2 versus MAP (R =0.55). Conclusions: Near-infrared spectroscopy- measured rSO 2 provided reproducible decreases during hemorrhage that were similar in time course to invasively measured cardiac output and SvO2 but delayed 5 to 9 minutes compared with MAP and pulse pressure. rSO 2 may provide an earlier warning of worsening hemorrhagic shock for prompt interventions in patients with trauma when continuous arterial BP measurements are unavailable. © 2012 Lippincott Williams & Wilkins.

Cuatro lecciones de William Brass

Conferencias dictadas por el profesor Brass en visita a CELADE, 2-5 noviembre 1975

Aplicaciones de los metodos para estimar la cabalidad de los registros a partir de la distribucion por edades de las muertes: a).de William Brass; b).de Samuel Preston; resumen del estudio

A la informacion sobre la distribucion por edades en las muertes registradas a partir de la edad de 5 anos en Chile, Costa Rica, Guatemala, Panama y Peru, se aplican los dos metodos propuestos para evaluar la cabalidad de los registros de defunciones. Los resultados obtenidos de aplicar estos metodos se presentan en graficos y cuadros, con aplicaciones hechas en los 5 paises

Bolivia: estimacion de la mortalidad a partir de la distribucion por edades de las muertes, 1951. Metodo de William Brass

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Costa Rica: estimacion de la mortalidad masculina adulta a partir de la orfandad de padre por medio de tecnicas de William Brass

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Determinacion de la mortalidad por medio de las tecnicas de William Brass, con especial referencia al sistema logito

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Peru: comprobacion del metodo de William Brass sobre la estimacion del nivel de la mortalidad en 1940, a partir de la proporcion: hijos sobrevivientes

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Dissolução e aleatoriedade: a estética do romance na obra Almoço nu de William S. Burroughs. -

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Barão de Camargos: a razão clientelista em Minas Gerais (1840-1853)

Pós-graduação em História - FCHS