Historia de las cosas de Ethiopia, en la qual se cuenta muy copiosamente, el estado y pote[n]cia del emperador della ... con otras infinitas particularidades, assi dela religio[n] de aquella ge[n]te, como de sus cerimonias / segun que de todo ello fue tes

Capitulares grab. xil.

Sermones sancti Vincentij fratris ordinis predicatorum de tempore, Pars hiemalis ; De sanctis

Datos de impresor tomados del IBE

Expositio î câtica cantic[orum] salomonis

Pie de imp. tomado de colofón

Historia de la provincia de Aragón de la Orden de Predicadores : desde su origen y principio hasta el año de mil y seyscientos : dividida en dos libros

Al final de la h. 7 consta : Barcelona, impreso en casa de Iayme Cendrat, 1599

Idea general de la policia o Tratado de policia sacado de los mejores autores que han escrito sobre este objeto ...

Según Palau, T.XXV, 348884, el autor es Valeriola Riambau

Margarita poetica

Descripción basada en: Tomado de Craviotto I, 2370

De propietatibus rerum

Descripción basada en: Tit. tomado del Craviotto I, 759

De consolatione philosophiae ; De disciplina scholarium

Descripción basada en: Ti. tomado de Craviotto I, 1116

Libro, o pratica en cirurgía

El grab. xil. del cuerpo humano de la h. 2, impresa por Joan Jofré, se encuentra también en: Cirurgia de Guido de Cauliaco, impresa en Valencia :en casa de Pedro Patricio :Vendense en casa de Francisco Miguel ...,1596

Glòria al Chúquer [Manuscrito]: cor

"A la Sociedad Coral El Micalet. Regalo del socio de la misma en prueba de cariño. Miguel Rosell"

Lo Túria y València [Manuscrito]: cor

Número 246 en el catálogo de Vicente Galbis e Hilari García

Casus longi super sexto libro Decretalium et super Clementinis [Texto impreso]

Tit. tomado del Craviotto II, 4895

Polymerase recognition of synthetic oligodeoxyribonucleotides incorporating degenerate pyrimidine and purine bases

A universal base that is capable of substituting for any of the four natural bases in DNA would be of great utility in both mutagenesis and recombinant DNA experiments. This paper describes the properties of oligonucleotides incorporating two degenerate bases, the pyrimidine base 6H,8H-3,4-dihydropyrimido[4,5-c][1,2]oxazin-7-one and the purine base N6-methoxy-2,6-diaminopurine, designated P and K, respectively. An equimolar mixture of the analogues P and K (called M) acts, in primers, as a universal base. The thermal stability of oligonucleotide duplexes were only slightly reduced when natural bases were replaced by P or K. Templates containing the modified bases were copied by Taq polymerase; P behaved as thymine in 60% of copying events and as cytosine in 40%, whereas K behaved as if it were guanine (13%) or adenine (87%). The dUTPase gene of Caenorhabditis elegans, which we have found to contain three nonidentical homologous repeats, was used as a model system to test the use of these bases in primers for DNA synthesis. A pair of oligodeoxyribonucleotides, each 20 residues long and containing an equimolar mixture of P and K at six positions, primed with high specificity both T7 DNA polymerase in sequencing reactions and Taq polymerase in PCRs; no nonspecific amplification was obtained on genomic DNA of C. elegans. Use of P and K can significantly reduce the complexity of degenerate oligonucleotide mixtures, and when used together, P and K can act as a universal base.

A peptide that inhibits hydroxyapatite growth is in an extended conformation on the crystal surface

Proteins play an important role in the biological mechanisms controlling hard tissue development, but the details of molecular recognition at inorganic crystal interfaces remain poorly characterized. We have applied a recently developed homonuclear dipolar recoupling solid-state NMR technique, dipolar recoupling with a windowless sequence (DRAWS), to directly probe the conformation of an acidic peptide adsorbed to hydroxyapatite (HAP) crystals. The phosphorylated hexapeptide, DpSpSEEK (N6, where pS denotes phosphorylated serine), was derived from the N terminus of the salivary protein statherin. Constant-composition kinetic characterization demonstrated that, like the native statherin, this peptide inhibits the growth of HAP seed crystals when preadsorbed to the crystal surface. The DRAWS technique was used to measure the internuclear distance between two 13C labels at the carbonyl positions of the adjacent phosphoserine residues. Dipolar dephasing measured at short mixing times yielded a mean separation distance of 3.2 ± 0.1 Å. Data obtained by using longer mixing times suggest a broad distribution of conformations about this average distance. Using a more complex model with discrete α-helical and extended conformations did not yield a better fit to the data and was not consistent with chemical shift analysis. These results suggest that the peptide is predominantly in an extended conformation rather than an α-helical state on the HAP surface. Solid-state NMR approaches can thus be used to determine directly the conformation of biologically relevant peptides on HAP surfaces. A better understanding of peptide and protein conformation on biomineral surfaces may provide design principles useful for the modification of orthopedic and dental implants with coatings and biological growth factors that are designed to enhance biocompatibility with surrounding tissue.

Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase

3-methyladenine (3MeA) DNA glycosylases remove 3MeAs from alkylated DNA to initiate the base excision repair pathway. Here we report the generation of mice deficient in the 3MeA DNA glycosylase encoded by the Aag (Mpg) gene. Alkyladenine DNA glycosylase turns out to be the major DNA glycosylase not only for the cytotoxic 3MeA DNA lesion, but also for the mutagenic 1,N6-ethenoadenine (ɛA) and hypoxanthine lesions. Aag appears to be the only 3MeA and hypoxanthine DNA glycosylase in liver, testes, kidney, and lung, and the only ɛA DNA glycosylase in liver, testes, and kidney; another ɛA DNA glycosylase may be expressed in lung. Although alkyladenine DNA glycosylase has the capacity to remove 8-oxoguanine DNA lesions, it does not appear to be the major glycosylase for 8-oxoguanine repair. Fibroblasts derived from Aag −/− mice are alkylation sensitive, indicating that Aag −/− mice may be similarly sensitive.