989 resultados para Engenharia genética


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This study addresses the question of attraction and retention of talent in companies that produce engineering projects in the area of oil and natural gas in the city of Natal. The objectives were to identify the mechanisms that these companies use to attract and retain talented professionals and what the relationship between these practices and performance of these organizations in the market. This is a case study of a qualitative nature which were included in the fullness of companies that work in that class in the capital Potiguar. Have been applied to the managers of these companies structured questionnaires with eleven issues orientativas based on theoretical reference adopted. The research finds that managers understand the word "talent", recognize the importance of the appreciation of its employees and the development of their innate abilities to better organizational performance, much due to the fact they are acting in a market of fierce competition. His companies - though not submit the formal procedures related to the subject in question - have mechanisms that can be characterized as the attraction and retention of talent. The relationships identified in this study are consistent with the results found in other studies and put the information here can serve as the basis for that other managers, including other areas, to reach excellence in their respective industries

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Apesar do regime político brasileiro permitir a real participação da população na formação da vontade pública, historicamente constatamos um desinteresse da população para com os assuntos políticos. Esse desinteresse para com a política no Brasil transcende diferenças sociais e raciais uma vez que, mesmo os considerados mais politizados raramente se interessam por algum tipo de participação política. No entanto, vemos no ano de 2013 uma quebra desse desinteresse político. Nesse ano, uma série de manifestações, que se seguem até os dias atuais, foram mobilizadas através da atuação de cidadãos por meio das Redes Sociais. Com a recente mobilização da população para com os assuntos políticos, acredita-se que as mesmas abrem uma possibilidade de trazer os assuntos políticos para o dia a dia do cidadão e, para isso, busca-se na disciplina da Semiótica e na Engenharia Semiótica maneiras de informar e interagir com a sociedade sobre temas de cunho político. Para atingir esse objetivo, o presente trabalho propõe o desenvolvimento de um conceito de aplicativo que utilize as informações veiculadas no site da Câmara dos Vereadores de Belo Horizonte. No conceito de aplicativo desenvolvido, os cidadãos poderão opinar sobre as notícias veiculadas no site, ter acesso às informações dos vereadores atualmente eleitos, aprovar ou rejeitar projetos de lei em tramitação e comentar essas informações. Para validar o conceito de aplicativo desenvolvido, será aplicado um questionário estruturado que se baseia no System Usability Scale afim de avaliar a usabilidade do conceito proposto esperando que esse, de fato, facilite a interlocução da sociedade sobre temas públicos.

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Neste atual cenário mundial, em que as mudanças acontecem em uma velocidade espantosa, novos desafios, novas tecnologias e novos atores aparecem e participam ativamente dessas mudanças. Esta pesquisa apresenta exatamente um desses novos atores, as crianças, as quais interagem cada vez mais cedo com essa diversidade tecnológica. A participação das crianças nesse cenário passa a ser mais ativa quando as mesmas começam a aprender linguagem de programação através de ambientes computacionais. Esses ambientes são avaliados através de sua metacomunicação com aplicação de métodos da Engenharia Semiótica. Assim, a pesquisa tenta compreender e avaliar o processo de metacomunicação usuário-sistema e a contribuição do mesmo no processo de aprendizagem infantil. Com o objetivo de verificarmos essas ocorrências de rupturas entre o ambiente de programação Scratch e seu público-alvo específico, aplicamos a metodologia denominada MAC – Método de Avaliação da Comunicabilidade –, uma técnica conhecida pela Engenharia Semiótica desde 1999. Diversos resultados foram percebidos, porém um merece destaque especial, em que percebemos que as mensagens foram, em sua maioria, identificadas e interpretadas pelos usuários finais. Por outro lado, ocorreram rupturas de comunicação em todos os voluntários, mesmo para aqueles que concluíram todas as tarefas.

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As pesquisas e as práticas em inteligência analítica na Engenharia de Software têm crescido nas últimas décadas. As informações contidas em um repositório de software podem auxiliar engenheiros de software em suas atividades durante todas as fases do desenvolvimento de software. O uso da inteligência analítica está ajudando os profissionais da Engenharia de Software a obterem informações relevantes do repositório de software, direcionando-os para melhores tomadas de decisões. Por se tratar de um bem intangível, pode ser difícil compreender as informações geradas pelo software. Este trabalho realizou um mapeamento sistemático da literatura sobre inteligência analítica na Engenharia de Software, o que propiciou a elaboração de um arcabouço conceitual para utilização da inteligência analítica capaz de auxiliar nas atividades da Engenharia de Software. Com a finalidade de validar este arcabouço conceitual, foi construído um protótipo de uma aplicação que analisou dados de um software livre. Tal protótipo foi validado e comentado por um grupo focal formado por desenvolvedores e gestores de projetos de software de uma grande empresa da área de Tecnologia da Informação. Concluiu-se que a inteligência analítica é fortemente utilizada durante a fase de manutenção e vem crescendo sua utilização na área de Gestão e na Prática Profissional. Constatou-se que os commits podem ser bons indicadores da evolução de software e que a ferramenta desenvolvida neste trabalho permite compreender o que está sendo alterado no sistema e por que a alteração ocorreu.

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O presente objeto de pesquisa tem como foco de estudos a Engenharia Social e a Segurança da Informação. Visa analisar as técnicas de ataque e defesas aos ambientes virtuais vulneráveis em empresas de pequeno, médio e grande porte. O estudo propõe aumentar as diretivas de segurança de forma a incluir mecanismos de defesas contra a Engenharia Social, considerando o pressuposto de que a organização conta com uma diretiva de segurança que estabelece metas, as práticas e os procedimentos que reconhece como necessários para proteger seus ativos informacionais, recursos e colaboradores contra-ataques tecnológicos ou físicos aplicados pela Engenharia Social. Apoia-se em teorias de Souza (2015), Watson, Masons e Ackroyd (2014), Mitnick e Simon (2003).   A presente pesquisa será por meio de estudo de casos múltiplos, de abordagem qualitativa e natureza aplicada à Engenharia Social e Segurança da Informação. Utilizam-se como instrumentos de pesquisa dois questionários estruturados e entrevistas, considerando como sujeitos Gestores de Tecnologia da Informação (TI) e Diretores de 10 empresas, convidados por meio de correio eletrônico corporativo. Pretende-se ao final desta pesquisa, sugerir ferramentas de Segurança da Informação, propor um modelo de proteção contra a Engenharia Social e comparar as técnicas de defesas contra Engenharia Social utilizadas entre as empresas pesquisadas além de desenvolver um modelo de Política de Segurança da Informação.

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Ranas clasificadas como ranas pipiens representan a un grupo de especies relacionadas. En este trabajo se compararon cuatro poblaciones de este grupo viviendo en Costa Rica en alturas y en medios ambientes diferentes en cuanto a morfología y compatibilidad reproductiva. No existe una diferencia significativa entre la forma del rostro en las poblaciones estudiadas, pero al tamaño promedio de ambos sexos disminuye a un “cline” (grado) con incremento de altura. Cruces artificiales indican una reducción significativa en la compatibilidad reproductiva entre la población más alta y las más bajas. Se propone que el cambio clinal en tamaño y la incompatibilidad reproductiva de la población alta representa una divergencia genética y posiblemente una especie nueva en proceso de formación. Se propone también que una “especiación” parecida pueda estar ocurriendo en otras montañas y volcanes de América Central en varios grupos de organismos.

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Droughts surfaced in 1877 as a crucial problem for the birthing Brazilian nation. Engineers, who formed the country's technical and scientific elite, took it upon themselves to study, understand and fight the problem through planned actions of intervention on space. This work, based on proposals and discussions contained in engineering magazines and reports, aims to provide elements for the comprehension of how these systematized actions against droughts, in the Iate nineteenth and early twentieth century, contributed to spatial analysis and the formation of a (then-inexistent) regional and territorial planning discipline in Brazi!. Engineers, by taking up the position of masterminds in the country's modernization, guaranteed for themselves personal economic stability, social prestige and political power. By understanding nature, either as a resource to be exploited or an adversary to national progress, they contributed to the delimitation of the region now known as the Northeast. By seeking to understand the drought phenomenon, they created knowledge about the space they sought to intervene on; by constructing their projects amid political and economical difficulty, they changed the organizational structures of cities and country in the northeast. The proposals for açudes (Iarge water reservoirs) allowed the fixation of population and the resistance against droughts; the roads - railroads and automotive roadways - connected the sertão to the capitais and the coast, speeding up help to the affected populations during droughts and allowing the circulation of goods so as to strengthen the local economies in normal rimes. The adopted practices and techniques, adapted from foreign experience and developed through trial and improvement, were consolidated as an eminently spatial intervention course, even if a theoretical body of regional or territorial planning wasn't formed in Brazil. Regional Planning proper was first applied in the country in the Northeast itself, in the 1950s, based off an economical view of reality in order to achieve development. The engineer's work prior tothat date, however, cannot be dlsconsldered. It was proved that, despite facing financial and political hurdles, engineers had a profound commitment to the problem and intended to act systematically to transform the economical and social relations in the region, in order to be victorious in their struggle against droughts

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The peaches and nectarines are highly appreciated by consumer, but it is climacteric fruits, with availability in the market in small time. It is necessary to invest to obtain genotypes with fruit quality and small perishability or that it presente less physiological disorders after storage. The aims of this work were i) to evaluate the genetic divergence among 40 peach and nectarine trees genotypes based on postharvest quality and select posible parents; ii) to evaluate the susceptibility to chilling injury in peaches and nectarines after cold storage; iii) to evaluate divergence of peaches and nectarines on the basis in the susceptibility for chiling injury and select superior genotypes; iv) evaluate the correlations between quality and susceptibility to chilling injury of peaches and nectarines v) select parents with the combination of lower susceptibility to chilling injury and higher quality fruit. The study was carried out in EEAD-CSIC, Zaragoza - Spain, during the production cycle 2013/2014. A total of 40 peaches and nectarines genotypes from germplasm collection were evaluated. The quality characteristics as flesh firmness, total soluble solids, titratable acidity, pH, rippining index and flesh color parameters were evaluated. The fruits were submitted to cold storage at 0 °C and 5 °C, with 95% average relative humidity. The evaluations were after 14 and 28 days, it being observed the presence of symptoms, such as wooliness through mealiness, flesh grainy, leatheriness and flesh color changes, through browning, bleeding and off flavor. As a selection parameter was adopted 20% of genotypes that had a higher frequency of superiority for quality characteristics, susceptibility to chilling injury and the combining of both. For quality characteristic presented greater divergence the ‘Queen Giant’, ‘Sudanel Blanco’ and ‘Borracho de Jarque’. Based on the quality the eight genotypes were selected, ‘Andross’, ‘San Jaime’, ‘San Lorenzo’, ‘Borracho de Jarque’, ‘Sudanell 1’, ‘Carson’, ‘Baby Gold 6’ and ‘Stanford’. All genotypes studied exhibited susceptibility to one or more symptoms caused by cold storage during 28 days, independent of temperature. For 14 days, the ‘Baby Gold 6’, ‘Flavortop’ and ‘Queen Giant’ genotypes did not show any physiological disorder caused by cold. In general, the temperature of 0 °C favored fruit postharvest conservation, it have a lower incidence and severity of symptoms caused by cold storage. The storage for 14 days contributed for the lower incidence of damage in the genotypes fruits studied. For 14 days, with both temperatures, it was observed divergence for ‘Queen Giant’, ‘Sudanell Blanco’, ‘Baby Gold 6’ ‘GF3’, ‘Baby Gold 8’, ‘Campiel’ and ‘Campiel Rojo’ genotypes. For 28 days, in the 5 °C condition, ‘Queen Giant’, ‘Big Top’, ‘Flavortop’ and ‘Redhaven’ genotypes were divergents. Based on susceptibility to chilling injury at 0 °C, the eight genotypes were selected, it being these, ‘Queen Giant’, ‘Keimoes’, ‘Flavortop’, ‘Big Top’, 'Redhaven', 'Sudanell 3', 'Bonet I' and ‘Carson’. The quality parameters as rippining index, soluble solids, firmness and titratable acidity presented correlation among them. These, also it had correlation with woolines and bowning, what it indicate that fruits with more ripening can have this symptoms more easily. The browning, mealiness, flesh grainy and off flavor variables were correlationed with the time period and temperartures, what it confirm that these symptoms are the main disorders caused by cold storage. The quality characteristics together susceptibility to chilling injury allowed selected ‘Baby Gold 6’, ‘Sarell’, ‘Keimoes’, ‘GF3’ ‘San Jaime’, ‘Big Top’, ‘Sudanell 1’, ‘Carson’, ‘Baby Gold 8’, and ‘San Lorenzo’ genotypes.

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To design strategies for the conservation and use of genetic resources of tree species such as jaboticaba tree, it is essential to make the characterization. In southwestern Paraná region, there are several forest fragments containing native jaboticaba tree (Plinia cauliflora), whose materials have broad potential for commercial orchards or breeding programs. As is the potential genetic diversity of a population to produce different genotypes, it would be able to start in such a characterization one of these fragments. The aim was to characterize fruits of jaboticaba tree (P. caulifora) of forest fragment kept in Clevelândia - PR for the presence of phenotypic variability, seeking to identify those superiors named for future selection as farming or male parent, as well as estimate genetic divergence between them, as a complementary tool for this purpose. Also, verify the regeneration and spatial distribution of the species. For the study was defined portion of a hectare (10.000 m²), with all individuals identified, mapped, with local coordinate system, and measured height and diameter. Fruits were characterized by sensory and biochemical characteristics in two years, 70 genotypes at 2013 and 56 at 2014, and of these 33 genotypes in both years. As a pre-selection criteria was adopted the choice of 20% of the genotypes that showed the highest frequency of superiority in the evaluated characteristics of the fruit. Genetic divergence among 33 genotypes per year was analyzed. The distribution pattern and spatial association was evaluated by Ripley's K function. It was classified for the first time the following ontogenetic stages of jaboticaba tree, by plant height, seedling (from 0.01 to 0.99 m), juvenile (1.0 to 4.99 m), immature (> 5.0 m, non-reproductive), adult (reproductive). It was also have been describe for the first time the naturally occurring juxtaposed seedlings, indicating polyembryony. The number of regenerating identified in the population (seedlings: n = 2163; juveniles: n = 330; immature: n = 59) was much larger than the number of adults (n = 132). The species showed reverse J-shaped size structure standard, with high concentration of regenerating. The regeneration distribution occurs in aggregate pattern and there is seedling-adult dependence, due seed dispersal and seedling emergence closest to mothers. The jaboticaba tree regeneration is sufficient to maintain the species for long term in this population, which should serve as reference to regeneration success for other studies of this important fruiting species from Ombrofile Mixed Forests. Has been pre-selected the jaboticaba trees 7, 42, 43, 47, 54, 91, 97, 104, 105, 118, 134, 153, 154, 157, 163, 169, 177, 186, 212, J7-01 and J7- 02, and 16 and 194 the ones that can now be selected by the superior characteristics of both cycles. It was recommended to carry out hybridization between genotypes 79 and 119, and 96 to 148. The quality of fruit analyzed showed potential for use as a dual purpose serving both in natura market or processing.

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This study aimed to assess the genetic inheritance, determine the better DNA isolation protocol for this species and to identify molecular markers associated with the Wild Poinsettia (Euphorbia heterophylla L.) resistance ALS- and PROTOX- inhibiting herbicides and. The genetic inheritance of resistance was determined from crosses between E. heterophylla biotypes susceptible (S) and resistant (R), backcrosses and F2 generation. The complete dominance of resistance was confirmed with dose response curves. Ten adjusted methods for DNA isolation described in the literature were tested. The specific primers for ALS and PROTOX genes were designed from the consensus DNA sequence of these genes, obtained by aligning the gene sequences of the species Manihot esculenta and Ricinus communis L. Additionally, it was assessed the transferability of twenty SSR (simple sequence repeat) markers designed for Manihot esculenta, because among the species of Euphorbiaceae with more developed SSRs markers, because it is the closest relative phylogenetic species of E. heterophylla. Regarding genetic inheritance, the frequencies observed in the F1, F2, RCs and RCr did not differ significantly from the expected frequencies for a trait controlled by two dominant genes for multiple resistance and a single dominant gene for simple resistance to ALS- and PROTOX-inhibiting herbicides. The similar levels of resistance to dosage up to 2000 g i.a. ha-1 of fomesafen and dosage up to 800 g i.a. ha-1 of imazethapyr observed in F1 (heterozygous) and homozygous R biotype confirm the complete dominance of resistance to PROTOX- and ALS-inhibiting herbicides, respectively. The 0.2%BME protocol allowed the isolation of 7,083 ng μL-1 DNA, significantly (P=0.05) higher than other methods. Co-isolation of phenolic compounds was observed in FENOL and 3%BME+TB methods, but the addition of polyvinylpyrrolidone (PVP40) in the protocol extraction buffer 3%BME+TA solved this problem. The primers designed for ALS and PROTOX genes amplified but not showed no visible polymorphism in agarose gel between the S and R biotypes of E. heterophylla. Regarding the SSR transferability, ten markers were transferred to E. heterophylla, however, these six primers showed polymorphism among S and R biotypes.

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Trabalho Final de Mestrado para obtenção do grau de Mestre em Engenharia Civil - Área de Especialização em Vias de Comunicações e Transportes

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Trabalho Final de Mestrado para obtenção do grau de Mestre em Engenharia Mecânica com Especialização em Energia, Climatização e Refrigeração