Induction of ankylosis in the incisor for orthodontic tooth movement in rats

Background/Aim: The present research evaluated the induction of ankylosis and the eruption rate in rat incisors, with the aim of achieving stable anchorage for orthodontic tooth movement (OTM) of the molars. Material and methods: Fifteen male Wistar rats were used. In the Experimental group, the right superior incisor of each animal was extracted, denuded of the PDL and the dental papilla, and reimplanted. The Control group was the left superior incisors. The eruption rate was measured at 7, 10, 12, 14, and 16 days after replantation. NiTi closed springs with a 50cN release force were installed for additional 9 days, and the eruption rate was determined. Then, the rats were sacrificed, and ankylosis was examined by microscopic analysis. Differences with P < 0.01 were defined as statistically significant. Results: The eruption rates were 0 and 0.39 mm day-1 in the Experimental group and Control group, respectively. All incisors of the Experimental group showed ankylosis according to histological analysis. Conclusions: The methodology used to induce ankylosis in this study was effective for anchoring the incisors during the OTM of rat molars, eliminating the undesirable effects consequent to continuous eruption. © 2013 John Wiley & Sons A/S.

Stimulation of MMP-1 and CCL2 by NAMPT in PDL cells

Periodontitis is an inflammatory disease caused by pathogenic microorganisms and characterized by the destruction of the periodontium. Obese individuals have an increased risk of periodontitis, and elevated circulating levels of adipokines, such as nicotinamide phosphoribosyltransferase (NAMPT), may be a pathomechanistic link between both diseases. The aim of this in vitro study was to examine the regulation of periodontal ligament (PDL) cells by NAMPT and its production under inflammatory and infectious conditions. NAMPT caused a significant upregulation of 9 genes and downregulation of 3 genes, as analyzed by microarray analysis. Eight of these genes could be confirmed by real-time PCR: NAMPT induced a significant upregulation of EGR1, MMP-1, SYT7, ITPKA, CCL2, NTM, IGF2BP3, and NRP1. NAMPT also increased significantly the MMP-1 and CCL2 protein synthesis. NAMPT was significantly induced by interleukin-1β and the periodontal microorganism P. gingivalis. NAMPT may contribute to periodontitis through upregulation of MMP-1 and CCL2 in PDL cells. Increased NAMPT levels, as found in obesity, may therefore represent a mechanism whereby obesity could confer an increased risk of periodontitis. Furthermore, microbial and inflammatory signals may enhance the NAMPT synthesis in PDL cells and thereby contribute to the increased gingival and serum levels of this adipokine, as found in periodontitis. © 2013 Marjan Nokhbehsaim et al.

Temporomandibular joint condylar changes following maxillomandibular advancement and articular disc repositioning

Purpose To evaluate condylar changes 1 year after bimaxillary surgical advancement with or without articular disc repositioning using longitudinal quantitative measurements in 3-dimensional (3D) temporomandibular joint (TMJ) models. Methods Twenty-seven patients treated with maxillomandibular advancement (MMA) underwent cone-beam computed tomography before surgery, immediately after surgery, and at 1-year follow-up. All patients underwent magnetic resonance imaging before surgery to assess disc displacements. Ten patients without disc displacement received MMA only. Seventeen patients with articular disc displacement received MMA with simultaneous TMJ disc repositioning (MMA-Drep). Pre- and postsurgical 3D models were superimposed using a voxel-based registration on the cranial base. Results The location, direction, and magnitude of condylar changes were displayed and quantified by graphic semitransparent overlays and 3D color-coded surface distance maps. Rotational condylar displacements were similar in the 2 groups. Immediately after surgery, condylar translational displacements of at least 1.5 mm occurred in a posterior, superior, or mediolateral direction in patients treated with MMA, whereas patients treated with MMA-Drep presented more marked anterior, inferior, and mediolateral condylar displacements. One year after surgery, more than half the patients in the 2 groups presented condylar resorptive changes of at least 1.5 mm. Patients treated with MMA-Drep presented condylar bone apposition of at least 1.5 mm at the superior surface in 26.4%, the anterior surface in 23.4%, the posterior surface in 29.4%, the medial surface in 5.9%, or the lateral surface in 38.2%, whereas bone apposition was not observed in patients treated with MMA. Conclusions One year after surgery, condylar resorptive changes greater than 1.5 mm were observed in the 2 groups. Articular disc repositioning facilitated bone apposition in localized condylar regions in patients treated with MMA-Drep. © 2013 American Association of Oral and Maxillofacial Surgeons.

Estudo comparativo com diferentes números de pontes na neurorrafia término-lateral em ratos

Pós-graduação em Bases Gerais da Cirurgia - FMB

Alterações odontológicas em pacientes urêmicos em hemodiálise e suas possíveis correlações com neuropatias de nervos cranianos

Pós-graduação em Bases Gerais da Cirurgia - FMB

Anatomia do aparelho reprodutor masculino de Dendropsophus minutus e Dendropsophus nanus (Anura, Hylidae) durante o ciclo reprodutivo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise morfométrica comparativa e biogeografia dos pequenos Artibeus brasileiros Leach, 1821 (Mammalia, Chiroptera, Phyllostomidae)

Pós-graduação em Biologia Animal - IBILCE

Implante venosos homólogo conservado em glutaraldeído da veia jugular em eqüinos: avaliação clínica, ultra-sonográfica e histopatológica

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Reimplante dentário tardio após o tratamento do ligamento periodontal necrosado com ácido cítrico

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Remoção mecânica do ligamento periodontal cementário necrosado com escova de Robinson e pedra pomes e com lâmina de bisturi. Análise histomorfométrica e MEV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Reimplante dentário tardio após o tratamento da superfície radicular com hipoclorito de sódio e fluoreto de sódio: análise histomorfométrica em ratos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise do processo de reparo do reimplante de incisivos de ratos diabéticos após a manutenção dos dentes em leite

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do tratamento da superfície radicular com própole ou com flúor no reimplante tardio de dentes de ratos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo histomorfométrico do processo de reparo de incisivos de ratos diabéticos submetidos à luxação extrusiva

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)