The application of in vitro sperm competition test to evaluate the impact of ZP-derived peptides on fertilization capacity of cat sperm

The present study aimed to establish a sensitive in vitro assay to assess the binding capacity of cat spermatozoa. Cat oocytes and epididymal sperm cells were isolated from gonads and cultured for in vitro fertilization. Before fertilization, the sperm ce

Morphine withdrawal affects both delayed-escape behaviour in Morris water maze and hippocampal NR2A/2B expression ratio

Repeated low-dose morphine treatment facilitates delayed-escape behaviour of hippocampus-dependent Morris water maze and morphine withdrawal influences hippocampal NMDA receptor-dependent synaptic plasticity. Here, we examined whether and how morphine wit

Biodiversity values of different aquatic systems, habitats and organisms in relation to restoration and sustaining of biodiversity

Biodiversity values provide objective data and advice from which policy makes could assess the conservation options and determine optimal policies that would balance the needs of conservation with the socia-economic needs of the people in the area.

Building damage and repair due to leakage in a deep excavation

The north-south line in Amsterdam is being built underneath the historic centre of the city. Three deep stations are being constructed in deep excavations supported by diaphragm walls. During the excavation for Vijzelgracht station, leakage through the wall resulted in large settlements and damage to historic buildings, which threatened continuation of the project. The authors analysed the cause of the leakage and the damage to the buildings. With the application of robust preventative measures at two of the deep excavations it was possible to continue the project. This paper reports on the cause of the events, the damage to the buildings and the counter-measures taken. It includes lessons learned for the project and for the foundations industry.

Reproductive physiology of ribbon fish (Trichiurus lepturus)

The ribbon fishes ‘of the family Trichiuridac are represented as one of the most important food resources in Indian ocean. High density of the dominant species of ribbon fish (Trichiurus lepturus) in Oman sea and the 'Tillable catch in last yeas (more than 7000 tones per year) makes a trust area for studing their population biolog and stock assessment. As our knowledge on reproductive biology of this species has an important role on their fisheries management, as well as conservation of this stock from decline or over fishing, this research was held to determine some aspects of reproductive physiology of ribbon fish and the effects of environmental factors in gonadal cycle. The goals of the present thesis is to determine some aspects of reproductive physiology such as gonadosomatic index (GSI) , hepatosomatic index (HSI), condition factor (Ko, fecundity, sex ratio, size at first maturity, size at maturity (LM5O) and their relative hormonal & biochemical fluctuations. In this regards annual variation of sex hormones ic. estradiol 17-B, progestron, cortisol, testostrone and gonadotropins FSH (GTH-I) , LH (GTH-ll)I were measured ; gonadal histological studies were done by light & electron micrography. The research was carried out from April 1995 to January 19% in Ras Nleidani in the north part of Oman sea, and the environmental factors such as temperature, salinity, oxygen, rainfall and pH were measured. The effects of these parameters on reproductive cycle and hormonal fluctuationswere discussed by using correlation and principle component analysis (PCA). Female Ribbon fish reproductive strategy shows the same paterns of nonguarder marine teleosts. T. lepturus has more than one spawning season (existance of egges in different size in each month) and therfore it must have asynchronous ovaries and belong to continious spawners. GSI and HSI are good evidences for this type of reproductive patern. The testis of the lobular type , which is typical of most teleosts , is composed of numerous lobules which are separated from each other by a thin layer of fibrous connective tissue. GSI fluctuations revealed prolong- spawning time in males. There is significant increase in 17-13 estradiol. progestrone , cortisol and gonadotropins with maturity and prespawning period of female T lepturus. Plasma concentration of E2 and GTH II incresaed along with water temperature increasing (3300).. Spawning was observed from Nov. 1995 to Apr. 1996 in this species. Progestrone increased significantly with increasing rainfall in this season (P<0.01). Plasma cortisol levels increased with maturation and vitelpgenesis and also with the peak of spawning. From lenght-weight frequency and size distribution in each age groups and also minimum size at first maturity (52a cm) it would he concluded that T. lepturus must be matured at 2 years of age. Serum cholestrol and triglicerides significantly increased when maturation occured in this species. The relationship between alkaline phosphatase activity and hormonal fluctuations with maturity and vitelogenesis were discussed. Proximate compostion (muscle) shows significant variation with spawning period and maturity. Absolute individual fecundity (17420-159150) increased with body length and weight. Ultrastructural observations show dramatic variation in cell membrane (0ocyte membrane), yolk vesicles and, nucleolus dispersal in relation to maturity stages. fluctuations of gonadal hormones were discused in relation with vitelogenesis. Testosterone increased in males from Nov: to Mar. due to environmental impacts and spawning time. Sex ratio in different depth (10-40 m ,80-110 m) shows significnt differences in this ratio for two depths. In 10-40 m depth female shows dominant abundance to male in each months that may be due to their reproductive migration behaviour. The effects of temperature photoperiod and rainfall to maturity and spawning were discussed. According to -pawning period of T. leptunts in our sampling area it could be suggested that ribbon fish fi,theries must be restricted in the peak of spawning seasons (Feb. to Mar.) and in the spawning grounds (under 40 m depths). Other suggestions for population conservation have been mentioned.

Growth rates of Tilapia esculenta (Graham) and Tilapia zillii (Gervais) under cultivation in ponds at Nyegezi, Tanzania

Aquaculture in Tanzania is still on a subsistence level and most of the ponds are maintained as part time job. The ponds are too small, shallow and over crowded with stunted Tilapia spp. In the present paper the results of experiments conducted in ponds at Nyegezi with T. esculenta and T. zillii are presented. This was part of an overall project of developing techniques of fish cultures with Tilapia under the limited existing conditions at Nyegezi. In a mono - species culture experiement with Tilapia zillii in nine month's time an average size of 172.8 mm/115.0 g was attained. In another experiment with T. zillii and T. esculenta in thirteen month's time, T. zillii attained an average size of 180.2mm/106.6 g and T. esculenta 193.6 mm/118.8 g. In another experiment with intensive feeding schedule an average size of 179.3 mm/126.6 g was attained by T. zillii and 191.0 mm/125.0 g by T. esculenta in four month's time. A locally prepared supplimentary feed with local Brewery Waste and Fish Meal (10:1) was readily accepted by both species of Tilapia. T. zillii voraciously fed on Cabbage leaves, Cauliflower leaves, Chinese cabbage leaves, Cassava leaves and on the common weed Comalina sp. Though all the items mentioned above were readily accepted by T. zillii feeding with Comaltna sp. was the easiest and most convenient because of its availability. In an intensive feeding experiment with vegetable leaves/Comalina sp. and the locally prepared supplimentary feed the fishes attained table size in four months time. Cement cistens of 5 X 3 X 1½ m size could be conveniently used for breeding both species of Tilapia. T. zillii had semi adhesive eggs and they were deposited on the sides of the cement wall. The number of young ones in a brood ranged from 160 to 314 in T. esculenta and 687 to 4,356 in T. zillii.

宁夏新建二代林网主要害虫种群数量变动规律初探

本文在连续 3年 (1 993～ 1 995年 )大量调查的基础上 ,研究了宁夏新建二代林网主要造林树种的主要害虫种群数量消长动态和发生规律。结果表明 :新建林网中具有潜在危险性的主要害虫为 :光肩星天牛 (Anoplophora glabripennis Motsch.)、木蠹蛾 (Cossuscossus orientalis Gaede &H olcocerus vicarius(Walker) )、枝梢害虫 (槐花球蚧 Eulecaniumkuwanai (Kanda) .&扁平球坚蚧 Parthenolecanium orientalis(Bouch) )。光肩星天牛种群呈下降趋势 ;木蠹蛾种群为上升趋势 ;槐花球蚧种群虽有下降趋势 ,但虫口密度依然较高 ;扁平球坚蚧在白蜡纯林中种群有逐年增长的趋势 ,而在混交林内数量变化平中有降。研究发现各害虫在合理的树种混交方式下 ,种群数量变化幅度小 ,危害轻 ,因而认为生产中只有营造合理树种搭配下的混交林才能防止和控制害虫的发生。

Alternative approaches to slum upgrading in Kibera, Nairobi

This paper discusses the sustainability of two different approaches to upgrade water and sanitation infrastructure in Kenya’s largest informal settlement, Kibera. A background to the urbanization of poverty is outlined along with approaches to urban slums. Two case-studies of completed interventions of infrastructure upgrading have been investigated. In one case-study, the upgrading method driven by an NGO uses an integrated livelihoods and partnership technique at community level to create an individual project. in the other case-study, the method is a collaboration between the government and a multi-lateral agency to deliver upgraded services as a part of a country-wide programme. The ‘bottom-up’ (project) and ‘top-down’ (programme) approaches each seek sustainability and aim to achieve this in the same context using different techniques. This paper investigates the sustainability of each approach. The merits and challenges of the approaches are discussed with the projected future of Kibera. The paper highlights the valuable opportunity for the role of appropriate engineering infrastructure for sustainable urban development, as well as the alleviation of poverty in a developing context.