鸭儿湖摇蚊复组种群酯酶多态性的研究

应用淀粉凝胶电泳研究了从湖北鸭儿湖污染区采集的较耐污染的摇蚊复合组幼虫 ,结果发现 :在取样种群中存在高活性的 β酯酶 (EST) ,其电泳迁移率与致倦库蚊实验参考系均不同 ;从甘油醛 - 3 -磷酸脱氢酶 (GPD)染色结果发现 ,其酶谱与已报道的库蚊 GPD染色结果有很大不同 ,在污染最重的 1号氧化塘的摇蚊复合组幼虫的 GPD没有表达 ;但在中度污染的2、3号氧化塘中 ,摇蚊复合组幼虫 GPD位点呈现明显多态现象。群体遗传学分析结果表明 ,取样种群由于杂合子不足造成种群偏离哈迪 -温伯格平衡。所分析

湖北汉族人大肠癌HLA-DRB1等位基因遗传易感性研究

目的　探讨湖北汉族人 HL A- DRB1等位基因与大肠癌遗传相关性。方法　针对 HL A- DRB1等位基因第 2外显子多态性 ,设计 2 3对引物的序列特异性引物聚合酶链反应 ,结合等位基因序列分析 ,检测了无亲缘关系的湖北籍汉族健康人 136名及大肠癌患者 72例的 HL A- DRB1基因。 SAS软件进行数据处理。结果　湖北地区汉族人大肠癌患者与正常人比较 ,HL A- DRB1* 0 90 1等位基因分布频率 0 .2 2 92 vs0 .1397(P<0 .0 0 5 ,OR=2 .182

洪湖日本沼虾种群生长的研究

１９９４年５月至１９９５年６月对洪湖日本沼虾种群的生长进行了研究．日本沼虾群体的平均体长、平均体重在３－６月逐渐增大，之后逐渐降低，分别在８月、９月达到最小值，其后又逐渐增大，反映出世代交替的过程日本沼虾群体的生长存在明显的阶段性，第一阶段（当年８－１１月）的生长可以用Ｌｏｇｉｓｔｉｃ生长模式来描述：（♂）Ｌ_ｔ=６．８５１（１＋ｅｏ．~（０．３１８６－０．２３８６ｔ），Ｗｔ＝（７．５０／（１＋ｅ~（２．３８４５－０．３２５０ｔ）·（♀）Ｌｔ６．２５／（１＋~（０．２３７２－０．１９８２ｔ）Ｗｔ５．７９／

鳜鱼病毒病原的检出及组织病理分析

经对患暴发性传染病的鳜组织及病变细胞超薄切片的电镜观察，在细胞质、核及间隙中都有病毒颗粒。对该病毒进行了分离提纯，这是一种直径约为280um球形病毒。将粗提病毒液接种到培养细胞中，会引起细胞病变；病毒通过人工感染健康鳜，会引起鳜发病和死亡。对患病鳜进行解剖和组织病理观察，表明病鳜的5种器官都发生了病变。研究结果表明所分离的鳜病毒（Sinipercachuatsivirus，SCV）是引起鳜流行病的病原。

人工诱导异源四倍体、新四倍体及异源三倍体白鲫的细胞遗传学研究

作者比较了第一代异源四倍体鱼、异源三倍体鱼、新四倍体鱼与亲本白鲫、红鲫及其杂交一代的染色体组型。三种亲本的二倍体染色体数均为100，但其组型分组各有差异，白鲫染色体组型公式：12m＋36sm＋32st十20t,NF＝148，在亚中着丝点组中有一对特大的标记染色体；红鲫染色体组型：20m＋34sm＋26st＋20t,NF＝154；白鲫×红鲫杂种染色体组型：16m＋35sm＋29st＋20t,NF＝151，有一条与白鲫相似的特大标记染色体，证实其组型由白鲫和红鲫各提供一套染色体组组成。异源三倍体的染色体数为1

Change prediction using interface data

Managing change can be challenging due to the high levels of interdependency in concurrent engineering processes. A key activity in engineering change management is propagation analysis, which can be supported using the change prediction method. In common with most other change prediction approaches, the change prediction method has three important limitations: L1: it depends on highly subjective input data; L2: it is capable of modelling 'generalised cases' only and cannot be; customised to assess specific changes; and L3: the input data are static, and thus, guidance does not reflect changes in the design. This article contributes to resolving these limitations by incorporating interface information into the change prediction method. The enhanced method is illustrated using an example based on a flight simulator. © The Author(s) 2013.

近亲尖额溞Alona affinis(Leydig,1860)的若干生物学特征

本文测量计数了采自贵州省江口县凯土河边的154个近亲尖额溞的体长和怀卵量,并探讨了这个种群的一些生物学性状。根据形态特征,确定本种为指名模式亚种。

云南星云湖、杞麓湖大头鲤的生物学

本文是根据云南星云湖、杞麓湖中所产大头鲤进行一些生态习性的研究。大头鲤的年龄鉴定是依据鳞片上环片切割现象。依星云湖的标本实测平均体长,Ⅰ龄为135.12毫米,Ⅱ龄鱼为196.93毫米,Ⅲ龄鱼为241.92毫米,Ⅳ龄焦为288毫米。星云湖和杞麓湖大头鲤体长和体重的关系式分别为:W=0.00001283L~(3.0719),W=0.00002181L~(2.9895)。食性极为单纯,主要是以枝角类和桡足类为食。不同大小个体的食性差异不大。Ⅰ龄鱼部分达到性成熟,Ⅱ龄鱼全部性成熟。其中性成熟最小个体雌鱼体长为14

How neurons migrate: a dynamic in-silico model of neuronal migration in the developing cortex.

BACKGROUND: Neuronal migration, the process by which neurons migrate from their place of origin to their final position in the brain, is a central process for normal brain development and function. Advances in experimental techniques have revealed much about many of the molecular components involved in this process. Notwithstanding these advances, how the molecular machinery works together to govern the migration process has yet to be fully understood. Here we present a computational model of neuronal migration, in which four key molecular entities, Lis1, DCX, Reelin and GABA, form a molecular program that mediates the migration process. RESULTS: The model simulated the dynamic migration process, consistent with in-vivo observations of morphological, cellular and population-level phenomena. Specifically, the model reproduced migration phases, cellular dynamics and population distributions that concur with experimental observations in normal neuronal development. We tested the model under reduced activity of Lis1 and DCX and found an aberrant development similar to observations in Lis1 and DCX silencing expression experiments. Analysis of the model gave rise to unforeseen insights that could guide future experimental study. Specifically: (1) the model revealed the possibility that under conditions of Lis1 reduced expression, neurons experience an oscillatory neuron-glial association prior to the multipolar stage; and (2) we hypothesized that observed morphology variations in rats and mice may be explained by a single difference in the way that Lis1 and DCX stimulate bipolar motility. From this we make the following predictions: (1) under reduced Lis1 and enhanced DCX expression, we predict a reduced bipolar migration in rats, and (2) under enhanced DCX expression in mice we predict a normal or a higher bipolar migration. CONCLUSIONS: We present here a system-wide computational model of neuronal migration that integrates theory and data within a precise, testable framework. Our model accounts for a range of observable behaviors and affords a computational framework to study aspects of neuronal migration as a complex process that is driven by a relatively simple molecular program. Analysis of the model generated new hypotheses and yet unobserved phenomena that may guide future experimental studies. This paper thus reports a first step toward a comprehensive in-silico model of neuronal migration.

Influence of dew on biomass and photosystem II activity of cyanobacterial crusts in the Hopq Desert, northwest China

Dew is an important water source for desert organisms in semiarid and arid regions. Both field and laboratory experiments were conducted to investigate the possible roles of dew in growth of biomass and photosynthetic activity within cyanobacterial crust. The cyanobacteria, Microcoleus vaginatus Gom. and Scytonema javanicum (Kutz.) Born et Flah., were begun with stock cultures and sequential mass cultivations, and then the field experiment was performed by inoculating the inocula onto shifting sand for forming cyanobacterial crust during late summer and autumn of 2007 in Hopq Desert, northwest China. Measurements of dew amount and Chlorophyll a content were carried out in order to evaluate the changes in crust biomass following dew. Also, we determined the activity of photosystem II(PSII) within the crust in the laboratory by simulating the desiccation/rehydration process due to dew. Results showed that the average daily dew amount as measured by the cloth-plate method (CPM) was 0.154 mm during fifty-three days and that the crust biomass fluctuated from initial inoculation of 4.3 mu g Chlorophyll a cm(-2) sand to 5.8-7.3 mu g Chlorophyll a cm(-2) crust when dew acted as the sole water source, and reached a peak value of approximately 8.2 mu g Chlorophyll a cm(-2) crust owing to rainfalls. It indicated that there was a highly significant correlation between dew amounts and crust moistures (r = 0.897 or r = 0.882, all P < 0.0001), but not a significant correlation between dew and the biomass (r = 0.246 or r = 0.257, all P > 0.05), and thus concluded that dew might only play a relatively limited role in regulating the crust biomass. Correspondingly, we found that rains significantly facilitated biomass increase of the cyanobacterial crust. Results from the simulative experiment upon rehydration showed that approximately 80% of PSII activity could be achieved within about 50 min after rehydration in the dark and at 5 degrees C, and only about 20% of the activity was light-temperature dependent. This might mean that dew was crucial for cyanobacterial crust to rapidly activate photosynthetic activity during desiccation and rehydration despite low temperatures and weak light before dawn. It also showed in this study that the cyanobacterial crusts could receive and retain more dew than sand, which depended on microclimatic characteristics and soil properties of the crusts. It may be necessary for us to fully understanding the influence of dew on regulating the growth and activity of cyanobacterial crust, and to soundly evaluate the crust's potential application in fighting desertification because of the available water due to dew. (C) 2009 Published by Elsevier Ltd.

Otolith characteristics and age determination of an endemic Ptychobarbus dipogon (Regan, 1905) (Cyprinidae: Schizothoracinae) in the Yarlung Tsangpo River, Tibet

We describe the microincrements, checks and annuli in the lapilli of the schizothoracine Ptychobarbus dipogon, an endemic species of the Tibetan plateau. We collected samples in the Yarlung Tsangpo River and its tributaries on a monthly basis (from April 2004 to August 2006). We describe the shape features of the three pairs of otoliths and document the full trajectory of lapillus development. We found that five to seven checks were clearly visible in the opaque zone of the first annulus. The pattern of 21-23 daily growth increments within each check might be explained as a lunar-induced deposition. We counted between 137 and 154 increments within the first annulus. Annuli appeared as a sequence of gradually declining increment widths, whereas false rings were characterized by abrupt checks. Our oldest estimates were 23(+)years for males and 44(+) for females. The time of annulus completion was clearly between March and April each year using monthly marginal increments analysis. We consider the factors responsible for daily increment formation as an endogenous circadian rhythm. Environmental information, such as strong sunlight and cold water temperatures in the Tibetan Plateau, could reinforce the endogenous daily cycle. Our results provided important data addressing the ecology and population dynamics of P. dipogon.

Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis

Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNA was synthesized from the hypothalamus of Chinese sturgeon, and the full-length cDNAs of two somatostatin (SS) genes were cloned and sequenced. The first cDNA (AsSS1) encodes a 116-amino acid protein that contains the SS14 sequence at its C-terminal extremity. AsSS1 shows high identity to that of human and other vertebrates. The second cDNA (AsSS2) encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]-SS14 at its C-terminal extremity. Both the two SS mRNAs were expressed in brain and pituitary with different mRNA levels. But in peripheral tissues, AsSS2 was more widely distributed than AsSS1. High mRNA levels of AsSS2 were found in liver, kidney and heart, while low mRNA levels of AsSS2 were also detected in ovary. Throughout embryogenesis and early larval development only AsSS2 mRNAs were detected. Furthermore, in the hypothalamus of one to five year-old Chinese sturgeon, AsSS2 but not AsSS1 maintained stable expression. The mRNA distribution suggests that the Chinese sturgeon AsSS2 products play important physiological functions in adult fish as well as in cell growth and organ differentiation in embryo and larva development. (C) 2009 Elsevier Inc. All rights reserved.

Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)

Microcystins (MCs) are cyanobacterial toxins in water blooms that have received increasing attention as a public biohazard for human and animal health. Previous studies were mainly focused on the toxic effects on adult fish, rather than juvenile or larvae, and the response of fish immune system were usually neglected. This paper presents the first data of the effects of microcystin-LR (MC-LR) on transcription of several genes essential for early lymphoid development (Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha) and heat shock proteins (HSP90, HSP70, HSP60, HSP27) in zebrafish larvae. Relative changes of mRNA transcription were analyzed by real time PCR. The transcription of Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha were up-regulated when following exposure to 800 mu g/L MC-LR, which may indicate that specific lymphocytes differentiation and TCR/lg arrangement are induced to counteract the toxic effects of MC-LR. It was also interesting to note the dramatically increased transcription of HSP90. HSP70, HSP60 and HSP27, which may indicate their important roles as molecular chaperones under oxidative stress. (C) 2009 Elsevier B.V. All rights reserved.