1000 resultados para população microbiana


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Pós-graduação em Psicologia - FCLAS

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Pós-graduação em Agronomia (Proteção de Plantas) - FCA

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The physical (pH) and microbiological (psychotrofi c microorganisms and lactic bacteria) characteristics of beef outside round (m. Biceps femoris) injected (15%) with brines free of polyphosphates containing and sodium lactate or sodium lactate and sodium diacetate and liquid bovine plasma (PLL and PLO) or dehydrated bovine plasma (PDL, PDO) were evaluated along with beef cuts injected with brines free from plasma, but containing polyphosphates and bacteriostatic agents (CL and CO) and non injected beef cuts (IN), comprising seven treatments of cooked and vacuum packaged beef steaks stored under refrigeration (6ºC) during 43 days. No differences in pH were detected among raw or cooked injected treatments, although IN showed lower pH value in raw beef cuts. The addition of liquid or dehydrated bovine plasma did not affect the microbial load after whole muscles pasteurization, but increased the bacterial counts in cooked beef steaks during refrigerated storage, comparing to treatments with no plasma addition (CL and CO). The storage temperature (6ºC), usually found during commercialization of meat increased the microorganisms growth rate affecting the microbiological quality, especially when plasma was added to the brine.

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The most important role played by the enzyme Glucose- 6-Phosphate Dehydrogenase (G6PD) in erythrocyte metabolism is in generating energy and reducing power used to protect the cell against oxidative attack. G6PD deficiency is the erythroenzymopathy that most frequently causes hemolytic anemia, and more than 130 molecular variants have already been identified. The aim of this study was to analyze the genetic mutations in the G6PD-deficient adult males in the population of the region of Araraquara, São Paulo State. Out of 5087 male blood donors, 89 were deficient for G6PD, as confirmed by assaying the enzyme activity and electrophoresis on cellulose acetate. Thus, a frequency of 1.75% of G6PD-deficient patients was found, this value being similar to other investigations in São Paulo state. Molecular analysis was performed by amplification of genomic DNA with specific primers and digestion with restriction enzymes. In 96.6% of the patients, the G6PD A¯ variant was observed, with mutations at residues 376(A→G) and 202(G→A). Mean G6PD specific activity among the patients was 1.31 IU.g Hb-1.min-1 at 37ºC, that is 10.8% of the normal activity of the G6PD B enzyme. The variant forms G6PD A¯ 680(G→T) and 968(T→C) were not found. In 3.4% of the deficient individuals, the G6PD Mediterranean variant was found, with a mutation at 563(C→T). In these cases, mean enzymatic activity was 0.25 IU.g Hb-1.min-1 at 37ºC, or 2.1% of the enzymatic activity of G6PD B. The use of traditional techniques, allied to the identification of the different molecular variants, is important for the understanding of the structural and functional properties and hemolytic behavior of the red blood cells of the patient.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Objetivo: esta investigação tem como objetivo avaliar a prevalência de periodontite apical detectada na radiografia periapical e a relação entre a doença e a qualidade da obturação de canais radiculares realizada por estudantes de graduação. Métodos: foi investigada uma amostra aleatória de 131 prontuários de pacientes que haviam recebido tratamento do canal radicular na Faculdade de Odontologia de Araçatuba, Universidade Estadual Paulista. Realizou-se anamnese, radiografias e exame clínico quanto à sintomatologia dolorosa, presença e qualidade da restauração, bolsa periodontal, mobilidade dentária e trauma oclusal. Resultados: a porcentagem de sucesso obtido com o tratamento realizado pelos estudantes de graduação foi de 87,7%. Quando o tratamento foi adequado (43 casos), o percentual de sucesso foi de 90,69% (39 casos), enquanto para os tratamentos inadequados (14 casos), o percentual de sucesso foi de 78,57% (11 casos), sem diferença estatisticamente significativa (p>0,05). Não foi observada diferença estatisticamente significativa na taxa de sucesso relacionada ao sexo (p>0,05). A condição pulpar também não interferiu na taxa de sucesso (p>0,05). Conclusão: concluiu-se que o tratamento endodôntico foi satisfatório e alcançou uma taxa de sucesso adequada, em torno de 90% dos casos.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Concomitant with the aging process there is an increasing of incidence of chronic diseases. So, it emerges the need for adaptation in the health system that requires new skills of healthcare professionals in order to serve this population. Therefore, it is necessary to understand the aging process and establish preventive measures. These issues are still far from achieving the ideal, however, many government programs and universities have sought to improve themselves and adapt to this change through a plurality of actions and preventive or health promotion intervention which provide a more resolute and humanized care plan through a multidisciplinary approach.