Clonagem e expressão da glicoproteína transmembrana do vírus linfotrópico de células T humanas em sistema procarioto

HTLV-1 is the virus that causes T cell lymphoma/leukemia in adults and a neurological disorder known as HTLV-associated myelopathy or tropical spastic paraparesis. One of the transmission means is through contaminated blood and its byproducts. Because of the risk of HTLV-associated infections, screening for HTLV was introduced for Brazilian blood donors in 1993. Most of the diagnostic kits used in the national blood banks are bought from foreign companies. Brazil does not have the technology to produce this material and there is a need to produce diagnostic systems with national technology. In this study, we show the expression of gp21/HTLV-1 in Escherichia coli and its reactivity towards monoclonal antibodies and the antibodies of infected patients. Expressing these proteins is the first step towards obtaining diagnostic kits with Brazilian biotechnology.

Comparação entre as necessidades energéticas prescritas e administradas a pacientes em terapia nutricional enteral

Objective: evaluating the adequacy, prescription and energy supply of enteral nutrition therapy in hospitalized patients. Methods: was performed a retrospective survey of the evolution of TNE protocol of 59 patients hospitalized in a general hospital in Marília / SP / BR. Data collected included gender, age, clinical diagnosis, anthropometric and dietary data related to the prescribed dietary formula, route and method of administration of the enteral nutrition, the daily volume prescribed and administered, the daily amount of energy required and offered in the diet, as well as possible complications. Body mass index (BMI), triceps skinfold (TSF) and arm muscle circumference (AMC) were used to establish the nutritional status of the patients. The adequacy of ENT was done according to the daily energy requirement compared to the average energy received during the daily use of ENT. Results: The average found to the BMI was 21.4 kg/m 2, and no differences were found comparing male and female. The data for TSF and AMC were different between genders (p<0.05) and suggest a more severe muscle mass in relation to adipose tissue. The averaged to the energy requirements was 1642 kcal/day but the average of energy prescribed was 1045 kcal/day and the amount offered was 1035 kcal/day. There was a significant difference between the necessity and the supplied energy offered through enteral nutrition (p=0.00) as well between the energy required and prescribed (p =0.00), both were shorter than the necessity. There was no significant difference (p>0.05) in energy supply, volume of enteral nutrition prescribed and administered among eutrophic, underweight or overweight patients. Conclusion: The results of this study indicate that the prescription and energy supply were not based on the needs of these patients, causing a significant energy deficit, which can lead to worsening of the nutritional status of the same. In hospitalized patients in use of ENT, the energy requirement must be made by a qualified professional within the multidisciplinary team, based on the needs of each patient.

Qualidade de vida de cuidadores de pe ssoas com necessidades especiais

The quality of life of caregivers is a concern because it directly affects the quality of life of individuals dependent on such care. This study aimed to analyze and compare the quality of life in health of caregivers of people with special needs who attend a rehabilitation facility. Ninety caregivers of people with special needs who attend a rehabilitation facility participated in this study. For data collection two instruments were used: a questionnaire to determine the profile of the caregiver and the person with special needs and the WHOQOL-Bref questionnaire to measure quality of life. The results were significant only between the caregiver's quality of life in the physical domain and age of the person with special needs, indicating that the older a person with special needs, the more difficult and arduous is the act of caring. The result can indicate guidelines for caring for families of people with disabilities.

Bioética-sociobiologia. Neologismos oportunos? Interface da tecnociência com as ciências humanas e sociais

The article focuses on the creation of the neologisms Bioethics and Sociobiology, analyzing these two words which due to their composition evoke relations between biological sciences and human studies (ethics and sociology). Highlights that these two neologisms, in fact, were born already loaded by deep meanings, but in different senses. Emphasizes the importance of bioethics in the integration of scientific and humanistic cultures, beyond university statuary regulations. Concludes that Bioethics conveys also a suggestive message about how to create mechanisms and conditions for facing the challenges which scientific advancements, in their inexorability, present.

Aspectos fundamentais no desenvolvimento de sistemas microemulsionados contendo anfotericina B para uso oftálmico

As occurs with a number of drugs, the bioavailability of amphotericin B (AmB) used to treat fungal infections by the ocular route remains a great challenge to research scientists. In fact, the poor bioavailability of AmB is due mainly to the corneal barrier, which leads to a precorneal loss and consequent decrease in the absorption of this drug into the intraocular tissues. The toxicity associated with this molecule, together with its poor ability to penetrate the intact corneal epithelium, also represents a major drawback to its clinical use. New effective and safe drug vehicles for ocular delivery of AmB are therefore urgently needed. Microemulsions (MEs) seem to be an interesting system, owing to their transparent appearance, thermodynamic stability and favorable viscosity. Knowledge of the process of formation of AmB-containing MEs, as well as a good understanding of the physical chemistry of such systems, would provide reliable information on the best conditions for the use of these systems as eye drops. The goal of this research was thus to make an approach to this subject by reviewing the main studies on the use of MEs as delivery systems for AmB in topical eye treatment.

Dicionário terminológico dos termos fundamentais da linguagem da linguagem das produções telejornalísticas

Pós-graduação em Estudos Linguísticos - IBILCE

Em foco: professores de Artes e suas experiências com os materiais educativos Lá vai Maria Bem-vindo professor! arte br

Pós-graduação em Artes - IA

Análise do imprinting e da expressão dos genes H19 e IGF2 em células de Sertoli humanas apos exposição in vitro ao 2,3,7,8-Tetraclorodibenzo-p-dioxina (TCDD)

Pós-graduação em Biologia Geral e Aplicada - IBB

Adaptação de um instrumento para classificação de pacientes baseado nas necessidades individualizadas no cuidado de enfermagem do paciente pediátrico oncológico

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Atividade citotóxica, pró-apoptótica, genotóxica e mutagênica de nitensidina B em células tumorais cervicais humanas

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

O produto de moda para o portador de deficiência física: análise de desconforto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização da acessibilidade em espaços públicos: a ergonomia e o desenho universal contribuindo para a mobilidade de pessoas portadoras de necessidades especiais : estudo de casos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Processos excludentes no espaço intra-urbano: condição de vida, saúde e redes sociais dos chefes de família desempregados Presidente Prudente-SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Política fiscal e reestruturação da dívida pública no Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Necessidades formativas dos professores dos anos iniciais na sua inserção no exercício da docência

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)