Towards mitigation of greenhouse gases by small changes in farming practices: understanding local barriers in Spain.

Small changes in agricultural practices have a large potential for reducing greenhouse gas emissions. However, the implementation of such practices at the local level is often limited by a range of barriers. Understanding the barriers is essential for defining effective measures, the actual mitigation potential of the measures, and the policy needs to ensure implementation. Here we evaluate behavioural, cultural, and policy barriers for implementation of mitigation practices at the local level that imply small changes to farmers. The choice of potential mitigation practices relevant to the case study is based on a literature review of previous empirical studies. Two methods that include the stakeholders? involvement (experts and farmers) are undertaken for the prioritization of these potential practices: (a) Multi-criteria analysis (MCA) of the choices of an expert panel and (b) Analysis of barriers to implementation based on a survey of farmers. The MCA considers two future climate scenarios ? current climate and a drier and warmer climate scenario. Results suggest that all potential selected practices are suitable for mitigation considering multiple criteria in both scenarios. Nevertheless, if all the barriers for implementation had the same influence, the preferred mitigation practices in the case study would be changes in fertilization management and use of cover crops. The identification of barriers for the implementation of the practices is based on the econometric analysis of surveys given to farmers. Results show that farmers? environmental concerns, financial incentives and access to technical advice are the main factors that define their barriers to implementation. These results may contribute to develop effective mitigation policy to be included in the 2020 review of the European Union Common Agricultural Policy.

How can irrigated agriculture adapt to climate change? Insights from the Guadiana Basin in Spain

Climate change is already affecting many natural systems and human environments worldwide, like the semiarid Guadiana Basin in Spain. This paper illustrates a systematic analysis of climate change adaptation in the Guadiana irrigation farming region. The study applies a solution-oriented diagnostic framework structured along a series of sequential analytical steps. An initial stage integrates economic and hydrologic modeling to evaluate the effects of climate change on the agriculture and water sectors. Next, adaptation measures are identified and prioritized through a stakeholder-based multi-criteria analysis. Finally, a social network analysis identifies key actors and their relationships in climate change adaptation. The study shows that under a severe climate change scenario, water availability could be substantially decreased and drought occurrence will augment. In consequence, farmers will adapt their crops to a lesser amount of water and income gains will diminish, particularly for smallholder farms. Among the various adaptation measures considered, those related to private farming (new crop varieties and modern irrigation technologies) are ranked highest, whereas public-funded hard measures (reservoirs) are lowest and public soft measures (insurance) are ranked middle. In addition, stakeholders highlighted that the most relevant criteria for selecting adaptation plans are environmental protection, financial feasibility and employment creation. Nonetheless, the social network analysis evidenced the need to strengthen the links among the different stakeholder groups to facilitate the implementation of adaptation processes. In sum, the diagnostic framework applied in this research can be considered a valuable tool for guiding and supporting decision making in climate change adaptation and communicating scientific results.

Associations between macronutrient intake and serum lipid profile depend on body fat in European adolescents: the Healthy Lifestyle in Europe by Nutrition in Adolescence (HELENA) study

The present study aimed to investigate the relationships between macronutrient intake and serum lipid profile in adolescents from eight European cities participating in the HELENA (Healthy Lifestyle in Europe by Nutrition in Adolescence) cross-sectional study (2006–7), and to assess the role of body fat-related variables in these associations. Weight, height, waist circumference, skinfold thicknesses, total choles- terol, HDL-cholesterol (HDL-C), LDL-cholesterol, TAG, apoB and apoA1 were measured in 454 adolescents (44 % boys) aged 12·5–17·5 years. Macronutrient intake (g/4180 kJ per d (1000 kcal per d)) was assessed using two non-consecutive 24 h dietary recalls. Associations were evaluated by multi-level analysis and adjusted for sex, age, maternal education, centre, sum of four skinfolds, moderate-to-vigorous.

Resonadores piezoeléctricos de cizalladura: estudio de la formación de biofilms y caracterización de fluidos magneto‐reológicos

La presente tesis revisa y analiza algunos aspectos fundamentales relativos al comportamiento de los sensores basados en resonadores piezoeléctricos TSM (Thickness Shear Mode), así como la aplicación de los mismos al estudio y caracterización de dos medios viscoelásticos de gran interés: los fluidos magnetoreológicos y los biofilms microbianos. El funcionamiento de estos sensores está basado en la medida de sus propiedades resonantes, las cuales varían al entrar en contacto con el material que se quiere analizar. Se ha realizado un análisis multifrecuencial, trabajando en varios modos de resonancia del transductor, en algunas aplicaciones incluso de forma simultánea (excitación pulsada). Se han revisado fenómenos como la presencia de microcontactos en la superficie del sensor y la resonancia de capas viscoelásticas de espesor finito, que pueden afectar a los sensores de cuarzo de manera contraria a lo que predice la teoría convencional (Sauerbrey y Kanazawa), pudiéndonos llevar a incrementos positivos de la frecuencia de resonancia. Además, se ha estudiado el efecto de una deposición no uniforme sobre el resonador piezoeléctrico. Para ello se han medido deposiciones de poliuretano, modelándose la respuesta del resonador con estas deposiciones mediante FEM. El modelo numérico permite estudiar el comportamiento del resonador al modificar distintas variables geométricas (espesor, superficie, no uniformidad y zona de deposición) de la capa depositada. Se ha demostrado que para espesores de entre un cuarto y media longitud de onda aproximadamente, una capa viscoelástica no uniforme sobre la superficie del sensor, amplifica el incremento positivo del desplazamiento de la frecuencia de resonancia en relación con una capa uniforme. Se ha analizado también el patrón geométrico de la sensibilidad del sensor, siendo también no uniforme sobre su superficie. Se han aplicado sensores TSM para estudiar los cambios viscoelásticos que se producen en varios fluidos magneto-reológicos (FMR) al aplicarles distintos esfuerzos de cizalla controlados por un reómetro. Se ha podido ver que existe una relación directa entre diversos parámetros reológicos obtenidos con el reómetro (fuerza normal, G’, G’’, velocidad de deformación, esfuerzo de cizalla…) y los parámetros acústicos, caracterizándose los FMR tanto en ausencia de campo magnético, como con campo magnético aplicado a distintas intensidades. Se han estudiado las ventajas que aporta esta técnica de medida sobre la técnica basada en un reómetro comercial, destacando que se consigue caracterizar con mayor detalle algunos aspectos relevantes del fluido como son la deposición de partículas (estabilidad del fluido), el proceso de ruptura de las estructuras formadas en los FMR tanto en presencia como en ausencia de campo magnético y la rigidez de los microcontactos que aparecen entre partículas y superficies. También se han utilizado sensores de cuarzo para monitorear en tiempo real la formación de biofilms de Staphylococcus epidermidis y Eschericia coli sobre los propios resonadores de cristal de cuarzo sin ningún tipo de recubrimiento, realizándose ensayos con cepas que presentan distinta capacidad de producir biofilm. Se mostró que, una vez que se ha producido una primera adhesión homogénea de las bacterias al sustrato, podemos considerar el biofilm como una capa semi-infinita, de la cual el sensor de cuarzo refleja las propiedades viscoelásticas de la región inmediatamente contigua al resonador, no siendo sensible a lo que sucede en estratos superiores del biofilm. Los experimentos han permitido caracterizar el módulo de rigidez complejo de los biofilms a varias frecuencias, mostrándose que el parámetro característico que indica la adhesión de un biofilm tanto en el caso de S. epidermidis como de E. coli, es el incremento de G’ (relacionado con la elasticidad o rigidez de la capa), el cual viene ligado a un incremento de la frecuencia de resonancia del sensor. ABSTRACT This thesis reviews and analyzes some key aspects of the behavior of sensors based on piezoelectric resonators TSM (Thickness Shear Mode) and their applications to the study and characterization in two viscoelastic media of great interest: magnetorheological fluids and microbial biofilms. The operation of these sensors is based on the analysis of their resonant properties that vary in contact with the material to be analyzed. We have made a multi-frequency analysis, working in several modes of resonance of the transducer, in some applications even simultaneously (by impulse excitation). We reviewed some phenomena as the presence of micro-contacts on the sensor surface and the resonance of viscoelastic layers of finite thickness, which can affect quartz sensors contrary to the conventional theory predictions (Sauerbrey and Kanazawa), leading to positive resonant frequency shifts. In addition, we studied the effect of non-uniform deposition on the piezoelectric resonator. Polyurethane stools have been measured, being the resonator response to these depositions modeled by FEM. The numerical model allows studying the behavior of the resonator when different geometric variables (thickness, surface non-uniformity and deposition zone) of the deposited layer are modified. It has been shown that for thicknesses between a quarter and a half of a wavelength approximately, non-uniform deposits on the sensor surface amplify the positive increase of the resonance frequency displacement compared to a uniform layer. The geometric pattern of the sensor sensitivity was also analyzed, being also non-uniform over its surface. TSM sensors have been applied to study the viscoelastic changes occurring in various magneto-rheological fluids (FMR) when subjected to different controlled shear stresses driven by a rheometer. It has been seen that there is a direct relationship between various rheological parameters obtained with the rheometer (normal force, G', G'', stress, shear rate ...) and the acoustic parameters, being the FMR characterized both in the absence of magnetic field, and when the magnetic field was applied at different intensities. We have studied the advantages of this technique over the characterization methods based on commercial rheometers, noting that TSM sensors are more sensitive to some relevant aspects of the fluid as the deposition of particles (fluid stability), the breaking process of the structures formed in the FMR both in the presence and absence of magnetic field, and the rigidity of the micro-contacts appearing between particles and surfaces. TSM sensors have also been used to monitor in real time the formation of biofilms of Staphylococcus epidermidis and Escherichia coli on the quartz crystal resonators themselves without any coating, performing tests with strains having different ability to produce biofilm. It was shown that, once a first homogeneous adhesion of bacteria was produced on the substrate, the biofilm can be considered as a semi-infinite layer and the quartz sensor reflects only the viscoelastic properties of the region immediately adjacent to the resonator, not being sensitive to what is happening in upper layers of the biofilm. The experiments allow the evaluation of the biofilm complex stiffness module at various frequencies, showing that the characteristic parameter that indicates the adhesion of a biofilm for the case of both S. epidermidis and E. coli, is an increased G' (related to the elasticity or stiffness of the layer), which is linked to an increase in the resonance frequency of the sensor.

La arquitectura religiosa en Santiago de Chile 1850-1950: razones de las reminiscencias góticas

Históricamente la estrategia de localización de la infraestructura religiosa católica en Chile ha estado condicionada por intenciones destinadas a la captación y tutela de feligreses, proceso desarrollado a través de intervenciones que han generado externalidades difíciles de anticipar y posibles de visualizar en un marco temporal mayor. En este contexto la producción e instalación de los templos católicos en distintos lugares de la ciudad condicionaron los territorios y marcaron presencia en su afán por colonizar y mantener el dominio, mecanismo propio de la institución religiosa que se adaptó al medio y a las circunstancias político social para cumplir su misión y mantener el poder. En esta investigación se buscó identificar desde las edificaciones destinadas al culto, la relación escalar entre localización, modo de instalación y morfología que incluye la forma y técnica de construcción, reconociendo la manera cómo modifican los entornos, el efecto que provocan y las expectativas que abren a nuevas intervenciones. El escenario escogido para realizar el estudio correspondió a un período de la historia de la ciudad de Santiago de Chile no documentado en que las circunstancias políticas, económicas, sociales y culturales contribuyeron a que el modo de disposición de las edificaciones de culto condicionaron el desarrollo urbano y orientaron el crecimiento de la ciudad. El período en que se desarrolló el estudio correspondió a la época de mayor riqueza material y cultural de la historia del país y estuvo modelada por las intenciones y planes de desarrollo tendientes a consolidar lo que se ha denominado el modelo Republicano. Escenario en que el espacio público cobró interés y se transformó en el motor de los cambios e indicador de la escala de la ciudad, estableciendo las condiciones para que las distintas corrientes europeas se manifestaran en el hacer arquitectónico cambiando la imagen original de la ciudad colonial. Este proceso que ocurrió sobre la estructura existente, densificó la manzana a través de la subdivisión predial, que complementado con los avances tecnológicos permitieron mayores alturas de edificación, situación que dio origen a nuevas concepciones espaciales, posibles de desarrollar con los recursos materiales, intelectuales y técnicos disponibles. El estudio se centró en la centuria 1850-1950 analizando la forma como la infraestructura religiosa Católica se localizó en el tejido urbano adoptando distintas tipologías edilicias, en una época caracterizada por el eclecticismo arquitectónico. Específicamente se estudiaron las edificaciones que utilizaron reminiscencias góticas, identificando los casos más significativos, estableciendo el contexto en que se originaron e indagando en la significación que tuvieron por la función que cumplieron, considerando emplazamiento y conexión con el entorno construido, conformación, dimensiones, destino y patrocinios. El área de estudio se fundamentó por la concentración de construcciones con la tendencia historicista, producciones que en el corto y mediano plazo orientaron las transformaciones de la ciudad y por la presencia que conservan hasta estos días. Se observó la incidencia de la acción de la Iglesia Católica en las políticas de Estado, orientando las decisiones de planificación y ocupación del territorio, condicionando la localización de los templos a fin de conseguir las mayores coberturas de la población y el mayor rendimiento de las inversiones. En el contexto latinoamericano la construcción de iglesias, templos y catedrales con reminiscencias góticas fue una constante que caracterizó al último cuarto del siglo XIX y las tres primeras décadas del siglo XX. El estudio permitió conocer en términos cuantitativos y cualitativos la producción, destacando la morfología y materialidad caracterizada por la adopción de estrategias contemporáneas estructuralmente exigentes. Se observó la utilización de la arquitectura como un medio de acercamiento a la población, capaz de transformar a los edificios en símbolos barriales, que facilitaron la generación de identidad, al convertirse en los referentes materiales que se destacan, que se recuerdan y que asumen la representatividad de barrios, permaneciendo en el tiempo y convirtiéndose en íconos asociados a la cultura local. En síntesis, la instalación de las infraestructuras religiosas en la ciudad de Santiago fue consecuencia de un modo de producción, que entregó lugares de encuentro y símbolos para la ciudad, a través de la localización de los edificios que buscaron ocupar áreas visibles, relevantes y convocantes, lo que consiguieron mediante la instalación geográfica, central y equidistante de conglomerados residenciales consolidados o en vías de desarrollo, como también a través de edificaciones monumentales y siempre destacadas respecto de su entorno, lo que marcó exclusividad y dominio. La elección de tipos arquitectónicos fue coyuntural a los tiempos e inspirada en intelectuales y profesionales mayoritariamente foráneos que aportaron conocimientos y experiencia para auspiciar edificaciones que fueron reconocidas como señeras y precursoras de la época. La intensidad en el uso de los recursos que demandaron las exigentes estructuras obligó a la iglesia católica a establecer estrategias de financiación destinadas a suplir la falta de recursos provenientes del Estado. Para lo cual convocaron a distintos actores los que participaron desde sus fronteras y en función de sus intereses cubriendo desde el altruismo hasta la rentabilización de su patrimonio. La organización iglesia católica se comporta de la misma manera que cualquier otra organización: busca expandirse, concentrar, controlar y administrar. Busca codificar todo su entorno (Raffestin, 2011), busca trascender y materialmente lo logra desde sus construcciones. ABSTRACT Historically the location strategies of the catholic religious infrastructure in Chile has been conditioned by intentions aimed at attracting and guardianship of parishioners, process developed through interventions that have generated externalities difficult to anticipate and possible to visualize in a in a major temporary frame. In this context, the production and installation of the catholic churches in different places in the city determined the territories and marked presence in their quest to colonize and maintain domain, mechanism of the religious institution that was adapted to the environment and in the political and social circumstances to fulfill its mission and maintain power. This research sought to identify from the buildings intended for worship in a multi-scale relationship between location, the placement mode, morphology and shape and construction technique and the effect caused by them, the way how they alter the environments and the expectations that are open to new interventions. The chosen scenario for the study corresponded to a not documented period in the history of the city of Santiago de Chile in which political, economic, social and cultural circumstances contributed to the form of disposition of the buildings of worship that determined the urban development and guided the growth of the city. The study period was the epoch of largest material and cultural wealth of Chile history and it was modeled by the intentions and development plans tending to consolidate what has been named the Republican ideal. Scenario in which public space gained interest and became the engine of change and indicator of the scale of the city, establishing the conditions for the various European trends manifested themselves in transforming the original image of the colonial city. This process that took place on the existing structure, produced a higher density of the original blocks through the predial compartimentation, which supplemented with technological advances enabled greater building heights, a situation that gave rise to new spatial conceptions, possible of developing with the material resources, intellectuals and technicians available at the moment. The study focused on the century 1850-1950 by analyzing how the Catholic religious infrastructure was located in the urban fabric by adopting different typologies locality, in an epoch characterised by architectural eclecticism. Specifica lly, it is studied the buildings that used gothic reminiscences, identifying the most significant cases, establishing the context in which they originated and inquiring about the significance that they had for the role it played, considering location and connection to the built environment, shaping, dimensions, destination, and sponsorships. The study area was established by the concentration of buildings with this historicist trend, productions that in the short and medium term guided the transformation of the city and the presence that keeps up to these days. It was noted the incidence of the of the Catholic Church actions in guiding State policies, planning decisions and occupation of the territory, as well as conditioning the location of the temples in order to achieve greater coverage of the population and the greatest return on investment. In the context of Latin America the construction of churches, temples and cathedrals with reminiscences gothic was a constant that characterized the last quarter of the nineteenth century and the first three decades of the twentieth century. The study allowed to know in quantitative and qualitative terms the production of the period, highlighting the morphology and materiality characterized by contemporary and structurally challenging strategies. It was noted the use of architecture as a means of approaching to the population, able to transform buildings into neighborhood symbols, which facilitated the production of identity, to become the reference materials that stand out, that will be remembered and that assume the representativeness of neighborhoods, remaining in the time and becoming icons associated with the local culture. In synthesis, the installation of religious infrastructure in the city of Santiago was result of a mode of production, that provided meeting places and symbols for the city, through the location of buildings that sought to occupy visible, relevant and summoning areas, what they obtained by means of the geographical, central and equidistant installation of consolidated residential conglomerates cluster or about to development, as also through always outstanding and monumental buildings with respect to its surroundings, which marked uniqueness and domain. The choice of architectural types was related to the time and inspired by intellectuals and professionals mostly outsiders who contributed with expertise and experience to sponsor buildings were recognized as outstanding and precursor of the time. The intensity of use of the resources that requested the demanding structures forced to the Catholic church to establish funding strategies designed to compensate for the lack of funds from the State. For which convened to different actors who participated from their borders and depending on their interests covering from the altruism up to the rentabilización of its patrimony. The Catholic Church organization behaves the same way as any other organization: seeks to expand, focus, control and manage. Looking for coding the entire environment (Raffestin, 2011), seeks to transcend and succeeds materially from its building.

Fluorescence-based directed termination PCR: direct mutation characterization without sequencing

We describe a fluorescence-based directed termination PCR (fluorescent DT–PCR) that allows accurate determination of actual sequence changes without dideoxy DNA sequencing. This is achieved using near infrared dye-labeled primers and performing two PCR reactions under low and unbalanced dNTP concentrations. Visualization of resulting termination fragments is accomplished with a dual dye Li-cor DNA sequencer. As each DT–PCR reaction generates two sets of terminating fragments, a pair of complementary reactions with limiting dATP and dCTP collectively provide information on the entire sequence of a target DNA, allowing an accurate determination of any base change. Blind analysis of 78 mutants of the supF reporter gene using fluorescent DT–PCR not only correctly determined the nature and position of all types of substitution mutations in the supF gene, but also allowed rapid scanning of the signature sequences among identical mutations. The method provides simplicity in the generation of terminating fragments and 100% accuracy in mutation characterization. Fluorescent DT–PCR was successfully used to generate a UV-induced spectrum of mutations in the supF gene following replication on a single plate of human DNA repair-deficient cells. We anticipate that the automated DT–PCR method will serve as a cost-effective alternative to dideoxy sequencing in studies involving large-scale analysis for nucleotide sequence changes.

Superoxide Dismutase in Arabidopsis: An Eclectic Enzyme Family with Disparate Regulation and Protein Localization1

A number of environmental stresses can lead to enhanced production of superoxide within plant tissues, and plants are believed to rely on the enzyme superoxide dismutase (SOD) to detoxify this reactive oxygen species. We have identified seven cDNAs and genes for SOD in Arabidopsis. These consist of three CuZnSODs (CSD1, CSD2, and CSD3), three FeSODs (FSD1, FSD2, and FSD3), and one MnSOD (MSD1). The chromosomal location of these seven SOD genes has been established. To study this enzyme family, antibodies were generated against five proteins: CSD1, CSD2, CSD3, FSD1, and MSD1. Using these antisera and nondenaturing-polyacrylamide gel electrophoresis enzyme assays, we identified protein and activity for two CuZnSODs and for FeSOD and MnSOD in Arabidopsis rosette tissue. Additionally, subcellular fractionation studies revealed the presence of CSD2 and FeSOD protein within Arabidopsis chloroplasts. The seven SOD mRNAs and the four proteins identified were differentially regulated in response to various light regimes, ozone fumigation, and ultraviolet-B irradiation. To our knowledge, this is the first report of a large-scale analysis of the regulation of multiple SOD proteins in a plant species.

EAST by Northeast

The Eastern Academic Scholars’ Trust (EAST) is a shared print initiative involving 48 libraries across the Northeast. Initiated in 2012 with a planning grant from the Andrew W. Mellon Foundation, EAST addresses directly the growing need for academic libraries to ensure that monographs and journals of scholarly value are not inadvertently discarded as they undertake weeding and deselection programs to free up space for other library services. EAST is currently completing a large-scale analysis of collections across 40 of the participating libraries. This analysis will provide insight into both uniqueness and overlap across the libraries’ holdings and will result in agreements by the libraries to retain circulating monographs in their local collections for an agreed upon time period and to make those materials available to researchers and scholars from other EAST libraries. In parallel to this collection analysis, EAST is implementing validation sampling across the libraries to better understand volume availability and condition and the role they may plan in retention decisions. The project team has developed an innovative sampling methodology and tools to support the study. As the largest shared print initiative to date, this project will secure a substantial portion of the scholarly record that is held in the Northeast and positions EAST as an important component of the growing network of shared print initiatives nationally.

The current challenges of Color Physics and Chemistry

Day of Chemistry, Invited conference, San Alberto Magno 2014

The white gene and locomotor recovery from anoxia in Drosophila melanogaster

Locomotor recovery from anoxia is complicated and little is known about the molecular and cellular mechanisms regulating anoxic recovery in Drosophila. For this thesis I established a protocol for large-scale analysis of locomotor activity in adult flies with exposure to a transient anoxia. Using this protocol I observed that wild-type Canton-S flies recovered faster and more consistently from anoxia than the white-eyed mutant w1118, which carries a null allele of w1118 in an isogenic genetic background. Both Canton-S and w1118 are commonly used controls in the Drosophila community. Genetic analysis including serial backcrossing, RNAi knockdown, w+ duplication to Y chromosome as well as gene mutation revealed a strong association between the white gene and the timing of locomotor recovery. I also found that the locomotor recovery phenotype is independent of white-associated eye pigmentation, that heterozygous w+ allele was haplo-insufficient to induce fast and consistent locomotor recovery from anoxia in female flies, and that mini-white is insufficient to promote fast and consistent locomotor recovery. Moreover, locomotor recovery was delayed in flies with RNAi knockdown of white in subsets of serotonin neurons in the central nervous system. I further demonstrated that mutations of phosphodiesterase genes (PDE) displayed wild-type-like fast and consistent locomotor recovery, and that locomotor recovery was light-sensitive in the night in w1118. The delayed locomotor recovery and the light sensitivity were eliminated in PDE mutants that were dual-specific or cyclic guanosine monophosphate (cGMP)-specific. Up-regulation of cGMP using multiple approaches including PDE mutation, sildenafil feeding or specific expression of an atypical soluble guanylyl cyclase (Gyc88E) was sufficient to suppress w-RNAi induced delay of locomotor recovery. Taken together, these data strongly support the hypothesis that White transports cGMP and promotes fast and consistent locomotor recovery from anoxia.