Characterization and detection of naturally occurring antibodies against IL-1 alpha and IL-1 beta in normal human plasma.

During the development and testing of a radioreceptor assay (RRA) for human IL-1, we have detected and identified the presence of auto-antibodies to IL-1 in normal human plasma (NHP). The RRA is based on the competition between human 125I-labeled rIL-1 alpha and standard or unknown quantities of IL-1 alpha or IL-1 beta for binding to a limited amounts of IL-1 receptor (IL-1R) isolated from the EL4 mouse thymoma cell line. NHP from 20 out of 100 unselected blood donors were found to completely inhibit the binding of 125I-labeled IL-1 alpha to its receptor, suggesting the presence in these NHP samples of either abnormal amounts of IL-1 or of a factor binding to the 125I-labeled IL-1 alpha. Special care was taken to ascertain that the inhibitory factors were antibodies and not soluble IL-1 receptor antagonist. When plasma samples with inhibiting activity were incubated with labeled IL-1 alpha and chromatographed on a Sephadex G200 column, they were found to contain 125I-labeled complexes with an apparent molecular weight of 150-200kD. The IL-1 binding factor could be eliminated from plasma by incubation with protein A-Sepharose, suggesting that it consisted in IgG antibodies directed against IL-1. Furthermore, the antibody nature of the inhibiting factor was confirmed by its binding to purified rIL-1 coupled to Sepharose. Screening of 200 NHP samples by incubation with 100 pg of 125I-labeled IL-1 followed by precipitation with 12% of polyethylene glycol (PEG) confirmed that about 25% of NHP contain detectable IgG antibodies to IL-1 alpha, while only 2% of NHP contain antibodies to IL-1 beta. No correlation between the presence of these anti-IL-1 antibodies and any particular major histocompatibility complex or any pathological conditions was detected. We suggest that all serum samples assayed for IL-1 alpha or IL-1 beta content should be pretested with the PEG precipitation assay described here.

Evaluación de la calidad medio marino costero en la Bahía de Talara y aguas adyacentes, 15-17 abril 1997

Presenta los resultados obtenidos de la evaluación de la calidad del ecosistema marino costero de la bahía Talara y aguas adyacentes, realizada del 15 al 17 de abril de 1997, con el objetivo de establecer un análisis comparativo estacional (otoño) y evaluar la influencia de las descargas terrestres en el medio marino dentro del Programa de Vigilancia del Estado de Calidad Marino Costero. El objetivo principal del estudio es continuar con la vigilancia del estado de calidad acuática.

Lottery Action, Vol. 15, no. 15, August 4, 17, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Lottery Action, Vol.15, no. 17, October 8-21, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Iowa Developments, December 2008, Vol. 17, no. 7

Business Development news from the Iowa Department of Economic Development

Concha de abanico (Argopecten purpuratus) en Bahía Independencia del 17 al 30 de mayo 1999

Presenta los resultados de la evaluación de la magnitud del stock de concha de abanico en la Bahía Independencia durante el mes de mayo de 1999, con el objeto de informar al Ministerio de Pesquería los estimados de población y biomasa para fines de manejo y ordenamiento pesquero. Se encontró el recurso en densidades ligeramente menores a las halladas en marzo del mismo año.

Interleukin-12p40 overexpression promotes interleukin-12p70 and interleukin-23 formation but does not affect bacille Calmette-Guérin and Mycobacterium tuberculosis clearance.

Interleukin (IL)-12p40, a subunit of IL-12p70 and IL-23, has previously been shown to inhibit IL-12p70 activity and interferon-gamma (IFN-gamma) production. However, recent evidence has suggested that the role of IL-12p40 is more complex. To study the contribution of IL-12p40 to immune responses against mycobacterial infections, we have used transgenic (tg) mice overexpressing IL-12p40 under the control of a major histocompatibility complex-II promoter. The IL-12p40 transgene was expressed during steady state at concentrations of 129 +/- 25 ng/ml of serum and 75 +/- 13 ng per spleen, while endogenous IL-12p40 was hardly detectable in control littermates. Bacille Calmette-Guérin (BCG) infection strongly induced the expression of IL-12p40 transgene in infected organs, and IL-12p40 monomeric and dimeric forms were identified in spleen of IL-12p40 tg mice. Excessive production of IL-12p40 resulted in a 14-fold increase in IL-12p70 serum levels in tg mice versus non-transgenic mice. IL-23 was also strongly elevated in the serum and spleens of IL-12p40 tg mice through BCG infection. While IFN-gamma and tumour necrosis factor protein levels were similar in IL-12p40 tg and non-transgenic mice, Th2 type immune responses were reduced in IL-12p40 tg mice. The number of BCG granulomas and macrophage expressing inducible nitric oxide synthase were similar in IL-12p40 tg and non-transgenic mice. IL-12p40 tg mice were as resistant as non-transgenic mice to BCG and Mycobacterium tuberculosis infections as they could efficiently control bacillary growth. These data show that high amounts of IL-12p40 promotes IL-12p70 and IL-23 formation, but that does not affect T helper 1 type immune responses and granuloma function, thus leading to normal mycobacterial clearance in infected organs.

Evaluación de la calidad del ambiente marino en la Bahía Ferrol - Chimbote (17 - 19 abril 1996)

Presenta los resultados de los parámetros de calidad de agua, de los elementos y compuestos químicos evaluados del 17 al 19 de abril en 1996. Se encontraron bajos valores de oxígeno y estados anóxicos, asimismo elevados sulfuros se registraron principalmente en la parte central de la bahía.

Nouveau régime des sociétés d'assurances sur la vie : commentaire des lois des 8 décembre 1904... 17 mars 1905... des décrets des 12 mai 1906... 9 juin 1906... 22, 25 juin 1906... suivi de tous les documents législatifs relatifs à la nouvelle législation (2e édition) / Jh Lefort,...

Collection : Collection du Bulletin-commentaire des lois nouvelles et décrets