Traços de personalidade, estados emocionais e condução : um estudo comparativo entre condutores de ambos os sexos

Tese de doutoramento, Ciências e Tecnologias da Saúde (Desenvolvimento Humano e Social), Universidade de Lisboa, Faculdade de Medicina, 2014

Gene Dosage Effect of WEE1 on Growth and Morphogenesis from Arabidopsis Hypocotyl Explants.

Background and Aims How plant cell-cycle genes interface with development is unclear. Preliminary evidence from our laboratory suggested that over-expression of the cell cycle checkpoint gene, WEE1, repressed growth and development. Here the hypothesis is tested that the level of WEE1 has a dosage effect on growth and development in Arabidospis thaliana. To do this, a comparison was made of the development of gain- and loss-of-function WEE1 arabidopsis lines both in vivo and in vitro. Methods Hypocotyl explants from an over-expressing Arath;WEE1 line (WEE1oe), two T-DNA insertion lines (wee1-1 and wee1-4) and wild type (WT) were cultured on two-way combinations of kinetin and naphthyl acetic acid. Root growth and meristematic cell size were also examined. Key Results Quantitative data indicated a repressive effect in WEE1oe and a significant increase in morphogenetic capacity in the two T-DNA insertion lines compared with WT. Compared with WT, WEE1oe seedlings exhibited a slower cell-doubling time in the root apical meristem and a shortened primary root, with fewer laterals, whereas there were no consistent differences in the insertion lines compared with WT. However, significantly fewer adventitious roots were recorded for WEE1oe and significantly more for the insertion mutant wee1-1. Compared with WT there was a significant increase in meristem cell size in WEE1oe for all three ground tissues but for wee1-1 only cortical cell size was reduced. Conclusions There is a gene dosage effect of WEE1 on morphogenesis from hypocotyls both in vitro and in vivo.

Perturbation of Cytokinin and Ethylene-signalling Pathways Explain the Strong Rooting Phenotype Exhibited by Arabidopsis Expressing the Schizosaccharomyces Pombe mitotic inducer, cdc25.

Background Entry into mitosis is regulated by cyclin dependent kinases that in turn are phosphoregulated. In most eukaryotes, phosphoregulation is through WEE1 kinase and CDC25 phosphatase. In higher plants a homologous CDC25 gene is unconfirmed and hence the mitotic inducer Schizosaccharomyces pombe (Sp) cdc25 has been used as a tool in transgenic plants to probe cell cycle function. Expression of Spcdc25 in tobacco BY-2 cells accelerates entry into mitosis and depletes cytokinins; in whole plants it stimulates lateral root production. Here we show, for the first time, that alterations to cytokinin and ethylene signaling explain the rooting phenotype elicited by Spcdc25 expression in Arabidopsis. Results Expressing Spcdc25 in Arabidopsis results in increased formation of lateral and adventitious roots, a reduction of primary root width and more isodiametric cells in the root apical meristem (RAM) compared with wild type. Furthermore it stimulates root morphogenesis from hypocotyls when cultured on two way grids of increasing auxin and cytokinin concentrations. Microarray analysis of seedling roots expressing Spcdc25 reveals that expression of 167 genes is changed by > 2-fold. As well as genes related to stress responses and defence, these include 19 genes related to transcriptional regulation and signaling. Amongst these was the up-regulation of genes associated with ethylene synthesis and signaling. Seedlings expressing Spcdc25 produced 2-fold more ethylene than WT and exhibited a significant reduction in hypocotyl length both in darkness or when exposed to 10 ppm ethylene. Furthermore in Spcdc25 expressing plants, the cytokinin receptor AHK3 was down-regulated, and endogenous levels of iPA were reduced whereas endogeous IAA concentrations in the roots increased. Conclusions We suggest that the reduction in root width and change to a more isodiametric cell phenotype in the RAM in Spcdc25 expressing plants is a response to ethylene over-production. The increased rooting phenotype in Spcdc25 expressing plants is due to an increase in the ratio of endogenous auxin to cytokinin that is known to stimulate an increased rate of lateral root production. Overall, our data reveal important cross talk between cell division and plant growth regulators leading to developmental changes.

Essential oils as anti-nematode agents and their influence on in vitro nematode

Tese de doutoramento, Biologia (Biotecnologia), Universidade de Lisboa, Faculdade de Ciências, 2015

Microbial community composition and mercury cycling in sediments of tagus estuary

Tese de doutoramento, Farmácia (Toxicologia), Universidade de Lisboa, Faculdade de Farmácia, 2016

Notation timelines and the aesthetics of disappearance

Contemporary cities are frequently surrounded by transitional landscapes: ambiguous lands, non-places on the urban edge, commonly experienced under the condition of speed. Although variously shaped by processes of urbanisation, logistics of road engineering, safety and ownership, and local people's lives, for travellers such landscapes are usually perceived in a state of disappearance. This condition presents a major challenge for the traditional methods used in architecture and urban design. For designers interested in the organisation and design of such mobility routes for the engagement of the traveller, a method of scripting based on notation timelines would provide a helpful supplement to traditional master plans. This paper explores the development of such a method and its roots in time-based arts, such as dance, music and film, as well as in the recent history of architecture and urban design. It does so through the presentation of an experimental study based on a real route, the train journey from London to Stansted airport.

The new suburban history, New Urbanism and the spaces in between

A review article of three recent books on suburbanization and suburbia in the USA: Andrew Friedman, Covert Capital: Landscapes of Denial and the Making of the US Empire in the Suburbs of Northern Virginia. Berkeley, Los Angeles: University of California Press, 2013. 416pp. £19.95 pbk. Elaine Lewinnek, The Working Man's Reward: Chicago's Early Suburbs and the Roots of American Sprawl. Oxford and New York: Oxford University Press, 2014. 239pp. 20 b&w illustrations. £30.99 hbk. Benjamin Ross, Dead End: Suburban Sprawl and the Rebirth of American Urbanism. Oxford and New York: Oxford University Press, 2014. 249pp. £20.99 hbk.

Meios de comunicação como instrumentos de inserção da comunidade cabo-verdiana na sociedade de acolhimento: o caso da área metropolitana de Lisboa

Dissertação apresentada à Escola Superior de Comunicação Social como parte dos requisitos para obtenção de grau de mestre em Jornalismo.

Transtextualidade na tradução de "Der mann im fahrstuhl / The mann in the elevator"

In an age where Babel has turned into transcultural communication, an interlingual approach – i.e. in English and in French – to the translating process from German to Portuguese appeared pertinent. Aiming at a refinement of the translating competence, this process consists in contrasting different linguistic and literary strategies through an intercultural and multi-etymological perspective. Thus, we settled upon Heiner Müller‘s play Der Auftrag. Erinnerung an eine Revolution (1980), on which the composer Heiner Goebbels has based himself to textually and musically dramatize an excerpt, Der Mann im Farhstuhl / The Man in the Elevator. A transcription of such excerpt in its source language, German, as well as its translation into English (Carl Weber, 1984, Performing Arts Publications, New York) and French (Jean Jourdheuil, Heinz Schwarzinger, Editions Minuit, Paris) can be found in the booklet that accompanies the CD – edited in 1988 by ECD (München: Records GmbH). It should be emphasized that such a creation allows a framing of Müller‘s text into a musical scenography and, therefore, encourages an intersemiotic contrast. This experience enabled us to come up with a unique imagery of Müller‘s piece of writing, by means of its dramatic and musical conversion and, simultaneously, lead us to stretch our textual consciousness to a multitude of intra-, extra- and interlinguistic elements.