1000 resultados para crestamento bacteriano do feijoeiro
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In Brazil, common bean is in one of the most representative economic crops, not only because of its growing area but also because of the economic value of its production. In the crop year 2006/07, in Selvíria (MS), we analyzed the common bean yield as a function of some physical attributes of a Typic Acrustox in minimum tillage and center pivot irrigation system. The aim of this work was to evaluate among the soil physical attributes, under minimum tillage, those that better explain the variability of bean yield using the Pearson and spatial correlations,. A geostatistical grid was installed to collect data from soil and plants, with 117 sampling points in an area of 2025 m² and homogeneous slope of 0.055 m m-1. The results showed a low yield of bean, which occurred probably due to a lower density of plants that the system provided. Thus, for the minimum tillage system, the bean yield could be explained as a function of total porosity and bulk density.
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The use of cover crops has been suggested as an effective method to maintain and/or increase the organic matter content, while maintaining and/or enhancing the soil physical, chemical and biological properties. The fertility of Cerrado soils is low and, consequently, phosphorus levels as well. Phosphorus is required at every metabolic stage of the plant, as it plays a role in the processes of protein and energy synthesis and influences the photosynthetic process. This study evaluated the influence of cover crops and phosphorus rates on soil chemical and biological properties after two consecutive years of common bean. The study analyzed an Oxisol in Selvíria (Mato Grosso do Sul, Brazil), in a randomized block, split plot design, in a total of 24 treatments with three replications. The plot treatments consisted of cover crops (millet, pigeon pea, crotalaria, velvet bean, millet + pigeon pea, millet + crotalaria, and millet + velvet bean) and one plot was left fallow. The subplots were represented by phosphorus rates applied as monoammonium phosphate (0, 60 and 90 kg ha-1 P2O5). In August 2011, the soil chemical properties were evaluated (pH, organic matter, phosphorus, potential acidity, cation exchange capacity, and base saturation) as well as biological variables (carbon of released CO2, microbial carbon, metabolic quotient and microbial quotient). After two years of cover crops in rotation with common bean, the cover crop biomass had not altered the soil chemical properties and barely influenced the microbial activity. The biomass production of millet and crotalaria (monoculture or intercropped) was highest. The biological variables were sensitive and responded to increasing phosphorus rates with increases in microbial carbon and reduction of the metabolic quotient.
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We studied the molecular epidemiology of Staphylococcus aureus strains potentially toxigenic, isolated from the production process of Minas frescal cheese in a small dairy plant in the state of São Paulo. For this, samples were taken during the period from June 2008 to July 2009. Samples were collected from the surface of the receiving and storage tanks of raw milk, the surface of the balance tank of pasteurized milk, the water supply system, the pipes and equipments, the hands of the handler and from the packaged cheese, totaling 140 samples. The colonies isolated on Baird-Parker Agar confirmed as Gram positive and positive for catalase, coagulase and acetoin production, were submitted to extraction of bacterial DNA using the Invitek - Uniscience® kit. Confirmation of the isolated species and enterotoxins SEA, SEB, SEC, SED and TSST-1 toxin was carried out through the amplification of specific fragments of chromosomal DNA. Among the 74 strains of isolated coagulase-positive staphylococci, only 41 (55.4%) strains were confirmed as Staphylococcus aureus, of which 25 (61.0%) were positive to the presence of staphylococcal toxins. The most frequently identified enterotoxin was SEA. The toxigenic strains of Staphylococcus aureus were more frequently isolated from hands of the handler (16.0%), raw milk receiving tank (12.0%), pasteurized milk for cheese making (12.0%) and fresh white cheese ready for consumption (12.0%).
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O objetivo deste trabalho foi avaliar o efeito do imunoestimulante β-glucano na dieta do tambaqui (Colossoma macropomum) sobre o desempenho produtivo, as respostas fisiológicas e imunológicas, e a resistência ao desafio com Aeromonas hydrophila. O experimento foi conduzido em delineamento inteiramente casualizado, em arranjo fatorial 5x2, com cinco níveis de β-glucano na dieta (0, 0,1, 0,2, 0,4 e 0,8%) e dois tempos de amostragem (antes e após o desafio com A. hydrophila), com três repetições. Os peixes (28,65±0,49 g; 12,14±0,07 cm) foram alimentados, por 60 dias, com dieta (28% de proteína bruta) suplementada com preparação comercial de β-glucano. Após o período de alimentação, avaliou-se o desempenho produtivo, e os peixes foram desafiados com A. hydrophila. Os parâmetros hematológicos e imunológicos (concentração e atividade de lisozima) foram avaliados antes e após o desafio bacteriano. Após o desafio bacteriano, observouse a ocorrência de anemia normocítica-normocrômica. A suplementação com β-glucano não alterou a concentração nem a atividade da lisozima; porém, a menor concentração de β-glucano (0,1%) favoreceu maior sobrevivência para a espécie quando desafiada com Aeromonas hydrophila. A suplementação de β-glucano não exerce influência sobre o desempenho produtivo e nem sobre os parâmetros hematológicos do tambaqui.
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Pós-graduação em Biotecnologia - IQ
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Pós-graduação em Doenças Tropicais - FMB
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Pós-graduação em Química - IQ
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Pós-graduação em Agronomia (Agricultura) - FCA
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Pós-graduação em Agronomia (Agricultura) - FCA
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The objective of this study was to evaluate the ingestive behavior of dairy cows fed corn and millet silage with a 5 mm particle size without inoculant, millet silage with a 5 mm particle size with inoculant, millet silage with a 20 mm particle size without inoculant, millet silage with a 20 mm particle size with inoculant. Five cows Holstein x Gir, with approximately 100 days of lactation at the beginning of the experiment and a mean body weight of 550 kg, producing an average of 15 kg of milk per day were used. The animals were arranged in a randomized 5 x 5 Latin square. The total feeding time, rumination time, chewing time, number of alimentary bolus, rumination time for bolus and number of chews was determined. The variables that make up the feeding behavior were not affected when the cows were fed different millet silages compared with corn silage. Cows fed corn silage and millet silage of 5 mm particle size had a higher intake of dry matter and neutral detergent fiber. A particle size between 5 and 20 mm, the presence or absence of inoculum and the type of silage with DM at 28%, does not affect the total feeding time, ruminating and resting of cows with an average production of 15.2 kg per day. The presence of the inoculant does not affect the intake of dry matter and neutral detergent fiber. Cows fed diets containing corn silage or pearl millet silage with a 5 mm particle size fed more DM.
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Pós-graduação em Biometria - IBB
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Aquicultura - FCAV
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Pós-graduação em Odontologia - FOAR
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A cárie dental é uma das doenças crônico-infecciosas mais comuns no mundo e é potencializada por fatores que favorecem a colonização da bactéria Streptococcus mutans na cavidade oral. O presente trabalho visa avaliar o potencial de sistemas nanoestruturados mucoadesivos para administração bucal do peptídeo sintético p1025, potencialmente ativo contra cárie dental. Este peptídeo, análogo aos fragmentos 1025-1044 da adesina celular de S. mutans mostrou-se, em estudos recentes, eficaz contra a adesão do patógeno na superfície do biofilme bacteriano. Acredita-se que, se incorporado em sistemas nanoestruturados mucoadesivos, sobretudo os sistemas líquido-cristalinos, sua ação possa ser modulada, pelo fato de que estes sistemas podem se aderir na mucosa bucal, de modo a proteger o peptídeo da degradação enzimática, além de prolongar o tempo de contato com a mucosa, diminuindo assim a frequência de administração. Os sistemas nanoestruturados de liberação controlada foram analisados estruturalmente através de microscopia de luz polarizada, determinação do comportamento reológico, TPA e bioadesão. Os resultados evidenciaram, através das análises de microscopia de luz polarizada, a presença de sistemas líquido-cristalinos de fase hexagonal e lamelar, além de domínios de microemulsões. As análises reológicas mostraram que ao adicionar dispersões poliméricas na fase aquosa do sistema, características como pseudoplasticidade e tixotropia são favorecidas, o que pode facilitar a aplicação do produto na mucosa bucal. O teste de biodesão mostrou que o emprego de dispersões poliméricas contribuiu para a adesão na mucosa bucal, sendo os melhores resultados obtidos com dispersão de Policarbofil® a 0,5 %.O teste microbiológico demonstrou a potencialização do efeito inibidor/redutor da carga microbiana com a utilização de óleo de melaleuca na fase oleosa do sistema. Os resultados obtidos sugerem que o ...