La noción de persona como particular de base. La ontología de la adscripción de Strawson, un precedente del soi-même de Ricoeur

En este artículo se parte de las aporías en las que el conductismo lógico de Gilbert Ryle deja la adscripción de los estados subjetivos y de la intencionalidad y se examina el argumento que orienta la solución ontológica de Peter Strawson a través de su noción primitiva de persona como particular de base. Se muestra que la noción de persona de Strawson es una salida a las aporías del mentalismo y del conductismo que anticipa la ontología del soi-même de Paul Ricoeur, aunque sin alcanzar la densidad narrativa que los quiénes reciben en el filósofo francés a partir del paso de las acciones discretas a las prácticas. Asimismo se establecen nexos entre la ontología de la persona y los problemas epistemológicos de la acción en las ciencias sociales.

La noción de persona como particular de base. La ontología de la adscripción de Strawson, un precedente del soi-même de Ricoeur

En este artículo se parte de las aporías en las que el conductismo lógico de Gilbert Ryle deja la adscripción de los estados subjetivos y de la intencionalidad y se examina el argumento que orienta la solución ontológica de Peter Strawson a través de su noción primitiva de persona como particular de base. Se muestra que la noción de persona de Strawson es una salida a las aporías del mentalismo y del conductismo que anticipa la ontología del soi-même de Paul Ricoeur, aunque sin alcanzar la densidad narrativa que los quiénes reciben en el filósofo francés a partir del paso de las acciones discretas a las prácticas. Asimismo se establecen nexos entre la ontología de la persona y los problemas epistemológicos de la acción en las ciencias sociales.

La noción de persona como particular de base. La ontología de la adscripción de Strawson, un precedente del soi-même de Ricoeur

En este artículo se parte de las aporías en las que el conductismo lógico de Gilbert Ryle deja la adscripción de los estados subjetivos y de la intencionalidad y se examina el argumento que orienta la solución ontológica de Peter Strawson a través de su noción primitiva de persona como particular de base. Se muestra que la noción de persona de Strawson es una salida a las aporías del mentalismo y del conductismo que anticipa la ontología del soi-même de Paul Ricoeur, aunque sin alcanzar la densidad narrativa que los quiénes reciben en el filósofo francés a partir del paso de las acciones discretas a las prácticas. Asimismo se establecen nexos entre la ontología de la persona y los problemas epistemológicos de la acción en las ciencias sociales.

Regles des cinq ordres d'Architecture, de Vignole : ouurage dans lequel on donne vne idée de la Géométrie ... / par C. M. Delagardette ... ; ...cet ouurage a pour suite et comprend Les leçons élémentaires des ombres dans l'architecture, ..., par le même

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NADH-quinone oxidoreductase: PSST subunit couples electron transfer from iron–sulfur cluster N2 to quinone

The proton-translocating NADH-quinone oxidoreductase (EC 1.6.99.3) is the largest and least understood enzyme complex of the respiratory chain. The mammalian mitochondrial enzyme (also called complex I) contains more than 40 subunits, whereas its structurally simpler bacterial counterpart (NDH-1) in Paracoccus denitrificans and Thermus thermophilus HB-8 consists of 14 subunits. A major unsolved question is the location and mechanism of the terminal electron transfer step from iron–sulfur cluster N2 to quinone. Potent inhibitors acting at this key region are candidate photoaffinity probes to dissect NADH-quinone oxidoreductases. Complex I and NDH-1 are very sensitive to inhibition by a variety of structurally diverse toxicants, including rotenone, piericidin A, bullatacin, and pyridaben. We designed (trifluoromethyl)diazirinyl[3H]pyridaben ([3H]TDP) as our photoaffinity ligand because it combines outstanding inhibitor potency, a suitable photoreactive group, and tritium at high specific activity. Photoaffinity labeling of mitochondrial electron transport particles was specific and saturable. Isolation, protein sequencing, and immunoprecipitation identified the high-affinity specifically labeled 23-kDa subunit as PSST of complex I. Immunoprecipitation of labeled membranes of P. denitrificans and T. thermophilus established photoaffinity labeling of the equivalent bacterial NQO6. Competitive binding and enzyme inhibition studies showed that photoaffinity labeling of the specific high-affinity binding site of PSST is exceptionally sensitive to each of the high-potency inhibitors mentioned above. These findings establish that the homologous PSST of mitochondria and NQO6 of bacteria have a conserved inhibitor-binding site and that this subunit plays a key role in electron transfer by functionally coupling iron–sulfur cluster N2 to quinone.

β-Trcp couples β-catenin phosphorylation-degradation and regulates Xenopus axis formation

Regulation of β-catenin stability is essential for Wnt signal transduction during development and tumorigenesis. It is well known that serine-phosphorylation of β-catenin by the Axin–glycogen synthase kinase (GSK)–3β complex targets β-catenin for ubiquitination–degradation, and mutations at critical phosphoserine residues stabilize β-catenin and cause human cancers. How β-catenin phosphorylation results in its degradation is undefined. Here we show that phosphorylated β-catenin is specifically recognized by β-Trcp, an F-box/WD40-repeat protein that also associates with Skp1, an essential component of the ubiquitination apparatus. β-catenin harboring mutations at the critical phosphoserine residues escapes recognition by β-Trcp, thus providing a molecular explanation for why these mutations cause β-catenin accumulation that leads to cancer. Inhibition of endogenous β-Trcp function by a dominant negative mutant stabilizes β-catenin, activates Wnt/β-catenin signaling, and induces axis formation in Xenopus embryos. Therefore, β-Trcp plays a central role in recruiting phosphorylated β-catenin for degradation and in dorsoventral patterning of the Xenopus embryo.

A single receptor encoded by vzg-1/lpA1/edg-2 couples to G proteins and mediates multiple cellular responses to lysophosphatidic acid

Extracellular lysophosphatidic acid (LPA) produces diverse cellular responses in many cell types. Recent reports of several molecularly distinct G protein-coupled receptors have raised the possibility that the responses to LPA stimulation could be mediated by the combination of several uni-functional receptors. To address this issue, we analyzed one receptor encoded by ventricular zone gene-1 (vzg-1) (also referred to as lpA1/edg-2) by using heterologous expression in a neuronal and nonneuronal cell line. VZG-1 expression was necessary and sufficient in mediating multiple effects of LPA: [3H]-LPA binding, G protein activation, stress fiber formation, neurite retraction, serum response element activation, and increased DNA synthesis. These results demonstrate that a single receptor, encoded by vzg-1, can activate multiple LPA-dependent responses in cells from distinct tissue lineages.

Does moderate alcohol consumption affect fertility? Follow up study among couples planning first pregnancy

Objective: To examine the effect of alcohol consumption on the probability of conception.

A zinc finger-containing papain-like protease couples subgenomic mRNA synthesis to genome translation in a positive-stranded RNA virus

The genome expression of positive-stranded RNA viruses starts with translation rather than transcription. For some viruses, the genome is the only viral mRNA and expression is regulated primarily at the translational level and by limited proteolysis of polyproteins. Other virus groups also generate subgenomic mRNAs later in the reproductive cycle. For nidoviruses, subgenomic mRNA synthesis (transcription) is discontinuous and yields a 5′ and 3′ coterminal nested set of mRNAs. Nidovirus transcription is not essential for genome replication, which relies on the autoprocessing products of two replicase polyproteins that are translated from the genome. We now show that the N-terminal replicase subunit, nonstructural protein 1 (nsp1), of the nidovirus equine arteritis virus is in fact dispensable for replication but crucial for transcription, thereby coupling replicase expression and subgenomic mRNA synthesis in an unprecedented manner. Nsp1 is composed of two papain-like protease domains and a predicted N-terminal zinc finger, which was implicated in transcription by site-directed mutagenesis. The structural integrity of nsp1 is essential, suggesting that the protease domains form a platform for the zinc finger to operate in transcription.