Complete protection against P. berghei malaria upon heterologous prime/boost immunization against circumsporozoite protein employing Salmonella type III secretion system and Bordetella adenylate cyclase toxoid

Sterile immunity against malaria can be achieved by the induction of IFNgamma-producing CD8(+) T cells that target infected hepatocytes presenting epitopes of the circumsporozoite protein (CSP). In the present study we evaluate the protective efficacy of a heterologous prime/boost immunization protocol based on the delivery of the CD8(+) epitope of Plasmodium berghei CSP into the MHC class I presentation pathway, by either a type III secretion system of live recombinant Salmonella and/or by direct translocation of a recombinant Bordetella adenylate cyclase toxoid fusion (ACT-CSP) into the cytosol of professional antigen-presenting cells (APCs). A single intraperitoneal application of the recombinant ACT-CSP toxoid, as well as a single oral immunization with the Salmonella vaccine, induced a specific CD8(+) T cell response, which however conferred only a partial protection on mice against a subsequent sporozoite challenge. In contrast, a heterologous prime/boost vaccination with the live Salmonella followed by ACT-CSP led to a significant enhancement of the CSP-specific T cell response and induced complete protection in all vaccinated mice.

The complete works of Count Rumford /

Green cloth binding, with gilt spine titles.

Complete songs and poems of Robert Tannahill, with life and notes.

Includes index.

The complete works of Samuel Rowlands, 1598-1628, now first collected ...

v.3. Heavens glory, seeke it, &c., 1628. The famous history of Gvy erle of Warwicke, 1682. Miscellaneous poems. Notes, by Sidney J.H. Herrtage. Glossary, by Sidney J.H. Herrtage.

Report of the commissioners of inland revenue on the duties under their management, for the years 1856 to 1869 inclusive; with some retrospective history, and complete tables of accounts of the duties from their first imposition ... Presented to both Houses of Parliament by command of Her Majesty.

Mode of access: Internet.

Handbook of the law of Mexican commercial corporations, including foreign corporations in Mexico (in English) containing explanations of the system of laws of the republic of Mexico and the law of the contract, management and operations of general corporations organized in Mexico; as well as of foreign corporations operating therein; together with complete forms for use by lawyers and corporate officials,

Mode of access: Internet.

UNIMATCH : a record linkage system : users manual /

Mode of access: Internet.