Comportamento de compra face à obsolescência planificada

No século XXI as fábricas produzem mais do que necessário, os fabricantes competem entre eles pelo mercado de consumo e são pressionados para vender. Para não ficarem com produtos em stock, recorrem a diversas técnicas de marketing, entre elas a inserção de obsolescência planificada nos seus produtos no ato da criação. A presente dissertação pretende estudar o comportamento de compra face à obsolescência planificada. O conceito de obsolescência planificada divide-se em três (3) tipologias: tecnológica, por qualidade, e psicológica. Quando um modelo mais recente do mesmo produto é colocado no mercado, este pode ser colocado com base em inovações tecnológicas reais ou melhoramentos superficiais fictícios. Em ambos os casos é considerado obsolescência tecnológica. Se um produto avaria antes do tempo ou é utilizada matéria-prima de qualidade inferior ou defeituosa, o fabricante introduziu obsolescência por qualidade no produto. Por fim, existe a obsolescência psicológica. Neste caso o problema não é do produto, pois continua funcional, mas sim do consumidor. Este está psicologicamente cansado do produto e opta pela sua substituição. O que se pretende estudar é o comportamento humano adotado face ao conceito. Terá a sociedade consumidora conhecimento deste assunto tabu? Este e outros fatores serão questionados e estudados nos capítulos seguintes.

Creación de un Instituto Tecnológico de Estudios Lacustres y Ambientales

Desde hace más de una década, el Lago San Pablo se ha convertido en un hermoso tema de investigación y diagnóstico; es decir, varias instituciones, sean estas, organizaciones gubernamentales o no gubernamentales han intervenido y han puesto su toque de gestión ambiental en los trabajos desarrollados, que parecen más bien – a la postre- una mera justificación a los recursos económicos manejados. El caso es, que sin desmerecer los logros teóricos alcanzados y el trabajo de concienciación logrado, los diagnósticos situacionales en los diferentes campos del accionar social, siendo problemas por resolver: la depredación del bosque nativo, la quema constante de los paramos, el desguarnecimiento de las vertientes, las concesiones de agua, la no existencia de inventarios de volúmenes de agua tanto superficial como subterránea, la contaminación, en fin, son múltiples las acciones por estudiar y emprender para lograr la Gestión Integral de cuencas hidrográficas andinas. Este es el hilo conductor que me lleva a plantear la creación de un Instituto Tecnológico de Estudios Lacustres y ambientales, como la forma más adecuada de poder ‘lograr’ la Gestión Integral de los recursos naturales en la cuenca del Lago San Pablo, con énfasis en los recursos hídricos, es decir, formar técnicos con conocimientos claros sobre la conducta del agua en un territorio determinado, donde además las actividades de los asentamientos humanos modifican el estado natural de los mismos e inciden directamente en lo que hoy conocemos como ‘calidad de vida’. Siguiendo los procedimientos que la Ley de Educación Superior y el respectivo Reglamento, así como otros documentos afines, pongo a consideración lo que podría ser una nueva institución de educación Tecnológica, con nuevas carreras para el desarrollo del País.

Memorias montoneras: metáforas de la guerra en Argentina (Crítica)

La autora lee la novela de Sergio Pollastri desde la perspectiva crítica de las posibilidades e imposibilidades de la reconstrucción de una historia, a partir de este relato testimonial que se mueve en el territorio de la violencia y las armas (la militancia política) y en el de la subjetividad (la poesía, la ficción). Resalta los vínculos entre vida y literatura, que cruzan toda la novela, así, apela a la metáfora para referirse a dos tipos de silencios en las ficciones sobre la violencia revolucionaria: la opción por las armas y la delación bajo tortura. Resalta el rol de la metáfora en este texto que tensa hasta sus límites el género testimonial, y que cobra su mayor fuerza hacia el final: «La revolución es frágil y superficial como una violeta, aquello que la comparación desarrolla es al mismo tiempo lo que amortigua la caída del salto brutal al vacío con el que la novela cierra su enunciación».

Análisis y propuesta de mejoramiento al sistema actual de evaluación del desempeño de la empresa Álvarez Barba S.A. – Quito, aplicando la metodología de modelamiento de perfiles por competencias enfocado al Área Comercial de vehículos BMW - Porsche

La evaluación de desempeño es un sistema que permite medir el rendimiento y productividad de los trabajadores de todas las áreas de negocio de una empresa; además que la información que se obtiene luego de la aplicación de este sistema es de gran importancia para comparar los niveles de cumplimiento individuales en relación con los objetivos generales de la Organización. Este subproceso del área de Recursos Humanos ayuda a potencializar el talento del capital humano, en especial el desarrollo de las competencias profesionales con las habilidades innatas de las personas. Este trabajo de investigación tiene como finalidad diagnosticar el sistema actual de evaluación y proponer un nuevo sistema de evaluación de desempeño basado en la metodología de competencias para poder determinar los factores que se deben fortalecer, como también desarrollar profesionalmente a los trabajadores que en este caso pertenecen al área comercial de vehículos BMW/PORSCHE. Esta metodología permite potencializar a los trabajadores otorgando una retroalimentación que comprende varios criterios como es el caso de la evaluación de 360º. La evaluación del desempeño a los empleados es un tema que se ha manejado de forma superficial que lo único que ha aportado es para desvincular o continuar en relación de dependencia con la empresa mas no con el desarrollo profesional de los mismos.

Desarrollo de un modelo de caracterización financiera del sector comercio en Ecuador, período 2002-2012

Caracterizar financieramente al sector comercial ecuatoriano involucra el investigar las principales variables macroeconómicas y financieras inmersas dentro del sector, para lo cual, se partió de la hipótesis de que el sector comercial ecuatoriano tiene un determinado desempeño financiero que se puede caracterizar a través de la construcción de un modelo estadístico. Se inició con la búsqueda de información sobre el desarrollo de modelos estadísticos similares, en tres países (Alemania, México y Chile), sin embargo, en la web superficial no se obtuvo un modelo a seguir pues únicamente se han estructurado modelos utilizando variables macroeconómicas. Por ello, se diseñó un modelo estadístico basado en el método de mínimos cuadrados ordinarios. La base de datos empleada corresponde al formulario 101 “impuesto a la renta de sociedades y establecimientos permanentes” del Servicio de Rentas Internas (SRI). El modelo estadístico trabaja con un nivel de confianza del 95%, existen once observaciones (período 2002-2012), siete variables independientes (exportaciones netas, inventario de materia prima y productos terminados, total costos y gastos, total ingresos, total pasivo, total patrimonio neto y ventas netas locales totales excluye activos fijos) que explican el comportamiento de la variable dependiente (utilidad del ejercicio). Se realizaron las pruebas de hipótesis correspondientes, presentando consistencia en los resultados encontrados. También se realizó la predicción del comportamiento de la variable dependiente por un período, la misma que difirió en 7,1% del valor real, lo que se explica por el limitado número de observaciones. Se construyeron dos escenarios (pesimista y optimista), asignándoles una probabilidad de ocurrencia, con lo que se calculó el valor esperado (US $ 3.727 millones) y la desviación estándar (US $ 4.842 millones). La investigación se compone de cinco capítulos. El primero, realiza una introducción y aborda la metodología. El segundo, refiere al marco teórico. El tercer capítulo, caracteriza estadística y financieramente al sector comercial ecuatoriano. En el cuarto capítulo se evalúan los efectos de la construcción del modelo utilizando el análisis de escenarios y de elasticidad. Mientras que el quinto capítulo recoge las conclusiones, observaciones y recomendaciones de la investigación.

Cellular and molecular investigations into the development of the pectoral girdle

The forelimbs of higher vertebrates are composed of two portions: the appendicular region (stylopod, zeugopod and autopod) and the less prominent proximal girdle elements (scapula and clavicle) that brace the limb to the main trunk axis. We show that the formation of the muscles of the proximal limb occurs through two distinct mechanisms. The more superficial girdle muscles (pectoral and latissimus dorsi) develop by the “In–Out” mechanism whereby migration of myogenic cells from the somites into the limb bud is followed by their extension from the proximal limb bud out onto the thorax. In contrast, the deeper girdle muscles (e.g. rhomboideus profundus and serratus anterior) are induced by the forelimb field which promotes myotomal extension directly from the somites. Tbx5 inactivation demonstrated its requirement for the development of all forelimb elements which include the skeletal elements, proximal and distal muscles as well as the sternum in mammals and the cleithrum of fish. Intriguingly, the formation of the diaphragm musculature is also dependent on the Tbx5 programme. These observations challenge our classical views of the boundary between limb and trunk tissues. We suggest that significant structures located in the body should be considered as components of the forelimb.

Researching from within: external and internal ethical engagement

This article examines the superficial and deep ethical and moral dilemmas confronting ‘insider’ researchers which we term external and internal ethical engagement. External ethical engagement refers to the traditional, easily identifiable ethical issues that insider researchers attend to by submitting their application for ethical approval to their institution’s internal review board. Internal ethical engagement relates to the deeper level ethical and moral dilemmas that insider researchers have to deal with once ‘in the field’ linked to on-going personal and professional relationships with participants, insider knowledge, conflicting professional and researcher roles, and anonymity. By reviewing the literature in this area and drawing on the authors’ experiences of undertaking interpretive studies at institutions where they were members of staff, we explore these concepts and examine the implications for insider researchers. Keywords: insider research; ethics; professional relationships; anonymity; access

‘That’s definite discrimination’: practice under the umbrella of inclusion

Inclusive practice is well embedded across society and has developed over time. However, although policy and public view have moved forward, the way organisations address the agenda for inclusion often represents a superficial interpretation of this concept. Qualitative data were gathered using new ethnography to explore the experiences of a library-based reading group for visually impaired readers. The voices of the individuals shed light on the individual and collective experience of reading. These insights challenge the traditional views of distinct provision that are designed to address targets for inclusion of individuals with disabilities. We argue for a clearer focus on the unintentional consequences of practice in the name of inclusion that leave individuals feeling marginalised. This paper suggests the alternative focus on social justice as offering a discourse that focuses on society and away from the individual.

Agonist-induced endocytosis of rat somatostatin receptor 1

Somatostatin-receptor 1 (sst1) is an autoreceptor in the central nervous system that regulates the release of somatostatin. Sst1 is present intracellularly and at the cell surface. To investigate sst1 trafficking, rat sst1 tagged with epitope was expressed in rat insulinoma cells 1046-38 (RIN-1046-38) and tracked by antibody labeling. Confocal microscopic analysis revealed colocalization of intracellularly localized rat sst1-human simplex virus (HSV) with Rab5a-green fluorescent protein and Rab11a-green fluorescent protein, indicating the distribution of the receptor in endocytotic and recycling organelles. Somatostatin-14 induced internalization of cell surface receptors and reduction of binding sites on the cell surface. It also stimulated recruitment of intracellular sst1-HSV to the plasma membrane. Confocal analysis of sst1-HSV revealed that the receptor was initially transported within superficial vesicles. Prolonged stimulation of the cells with the peptide agonist induced intracellular accumulation of somatostatin-14. Because the number of cell surface binding sites did not change during prolonged stimulation, somatostatin-14 was internalized through a dynamic process of continuous endocytosis, recycling, and recruitment of intracellularly present sst1-HSV. Accumulated somatostatin-14 bypassed degradation via the endosomal-lysosomal route and was instead rapidly released as intact and biologically active somatostatin-14. Our results show for the first time that sst1 mediates a dynamic process of endocytosis, recycling, and re-endocytosis of its cognate ligand.

Substance P released by TRPV1-expressing neurons produces reactive oxygen species that mediate ethanol-induced gastric injury

Although neurokinin 1 receptor antagonists prevent ethanol (EtOH)-induced gastric lesions, the mechanisms by which EtOH releases substance P (SP) and SP damages the mucosa are unknown. We hypothesized that EtOH activates transient receptor potential vanilloid 1 (TRPV1) on sensory nerves to release SP, which stimulates epithelial neurokinin 1 receptors to generate damaging reactive oxygen species (ROS). SP release was assayed in the mouse stomach, ROS were detected using dichlorofluorescein diacetate, and neurokinin 1 receptors were localized by immunofluorescence. EtOH-induced SP release was prevented by TRPV1 antagonism. High dose EtOH caused lesions, and TRPV1 or neurokinin 1 receptor antagonism and neurokinin 1 receptor deletion inhibited lesion formation. Coadministration of low, innocuous doses of EtOH and SP caused lesions by a TRPV1-independent but neurokinin 1 receptor-dependent process. EtOH, capsaicin, and SP stimulated generation of ROS by superficial gastric epithelial cells expressing neurokinin 1 receptors by a neurokinin 1 receptor-dependent mechanism. ROS scavengers prevented lesions induced by a high EtOH dose or a low EtOH dose plus SP. Gastric lesions are caused by an initial detrimental effect of EtOH, which is damaging only if associated with TRPV1 activation, SP release from sensory nerves, stimulation of neurokinin 1 receptors on epithelial cells, and ROS generation.

Recycling and resensitization of the neurokinin 1 receptor: influence of agonist concentration and Rab GTPases

Substance P (SP) induces endocytosis and recycling of the neurokinin 1 receptor (NK1R) in endothelial cells and spinal neurons at sites of inflammation and pain, and it is thus important to understand the mechanism and function of receptor trafficking. We investigated how the SP concentration affects NK1R trafficking and determined the role of Rab GTPases in trafficking. NK1R trafficking was markedly influenced by the SP concentration. High SP (10 nM) induced translocation of the NK1R and beta-arrestin 1 to perinuclear sorting endosomes containing Rab5a, where NK1R remained for >60 min. Low SP (1 nM) induced translocation of the NK1R to early endosomes located immediately beneath the plasma membrane that also contained Rab5a and beta-arrestin 1, followed by rapid recycling of the NK1R. Overexpression of Rab5a promoted NK1R translocation to perinuclear sorting endosomes, whereas the GTP binding-deficient mutant Rab5aS34N caused retention of the NK1R in superficial early endosomes. NK1R translocated from superficial early endosomes to recycling endosomes containing Rab4a and Rab11a, and Rab11aS25N inhibited NK1R recycling. Rapid NK1R recycling coincided with resensitization of SP-induced Ca2+ mobilization and with the return of surface SP binding sites. Resensitization was minimally affected by inhibition of vacuolar H(+)-ATPase and phosphatases but was markedly suppressed by disruption of Rab4a and Rab11a. Thus, whereas beta-arrestins mediate NK1R endocytosis, Rab5a regulates translocation between early and sorting endosomes, and Rab4a and Rab11a regulate trafficking through recycling endosomes. We have thus identified a new function of Rab5a as a control protein for directing concentration-dependent trafficking of the NK1R into different intracellular compartments and obtained evidence that Rab4a and Rab11a contribute to G-protein-coupled receptor recycling from early endosomes.

The syntax-semantics of bare and definite plural subjects in the L2 Spanish of English natives

This study investigates the extent to which advanced native-English L2 learners of Spanish come to acquire restrictions on bare plural preverbal subjects in L2 Spanish (e.g. gatos “cats” vs. definite plurals such as los gatos “the cats”). It tests L2 knowledge of available semantic readings of bare plurals and definite plurals in Spanish, where [+specific] and [+generic] interpretations are syntactically represented differently from English. Assuming L1 transfer, and in view of a potential subset/superset relationship of the two grammars, the learning task in this domain is not a straightforward one. Target acquisition requires both grammatical expansion and retraction; Spanish definite plural subjects require the addition of an L1-unavailable [+generic] reading, while a loss of an L1-available [+generic] reading for preverbal subject bare plurals is required. The results and analysis show that advanced L2 learners of Spanish (English L1) can circumvent a superficial subset/superset learnability problem by means of feature resetting in line with the Nominal Mapping Parameter.

Is optical imaging spectroscopy a viable measurement technique for the investigation of the negative BOLD phenomenon? A concurrent optical imaging spectroscopy and fMRI study at high field (7T)

Traditionally functional magnetic resonance imaging (fMRI) has been used to map activity in the human brain by measuring increases in the Blood Oxygenation Level Dependent (BOLD) signal. Often accompanying positive BOLD fMRI signal changes are sustained negative signal changes. Previous studies investigating the neurovascular coupling mechanisms of the negative BOLD phenomenon have used concurrent 2D-optical imaging spectroscopy (2D-OIS) and electrophysiology (Boorman et al., 2010). These experiments suggested that the negative BOLD signal in response to whisker stimulation was a result of an increase in deoxy-haemoglobin and reduced multi-unit activity in the deep cortical layers. However, Boorman et al. (2010) did not measure the BOLD and haemodynamic response concurrently and so could not quantitatively compare either the spatial maps or the 2D-OIS and fMRI time series directly. Furthermore their study utilised a homogeneous tissue model in which is predominantly sensitive to haemodynamic changes in more superficial layers. Here we test whether the 2D-OIS technique is appropriate for studies of negative BOLD. We used concurrent fMRI with 2D-OIS techniques for the investigation of the haemodynamics underlying the negative BOLD at 7 Tesla. We investigated whether optical methods could be used to accurately map and measure the negative BOLD phenomenon by using 2D-OIS haemodynamic data to derive predictions from a biophysical model of BOLD signal changes. We showed that despite the deep cortical origin of the negative BOLD response, if an appropriate heterogeneous tissue model is used in the spectroscopic analysis then 2D-OIS can be used to investigate the negative BOLD phenomenon.

An investigation to enhance understanding of the stimulation of weed seedling emergence by soil disturbance

Enhanced understanding of soil disturbance effects on weed seedling recruitment will help guide improved management approaches. Field experiments were conducted at 16 site-years at 10 research farms across Europe and North America to (i) quantify superficial soil disturbance (SSD) effects on Chenopodium album emergence and (ii) clarify adaptive emergence behaviour in frequently disturbed environments. Each site-year contained factorial combinations of two seed populations (local and common, with the common population studied at all site-years) and six SSD timings [0, 50, 100, 150, 200 day-degrees (d°C, base temperature 3°C) after first emergence from undisturbed soil]. Analytical units in this study were emergence flushes. Flush magnitudes (maximum weekly emergence per count flush) and flush frequencies (flushes year 1) were compared between disturbed and undisturbed seedbanks. One year after burial, SSD promoted seedling emergence relative to undisturbed seedbanks by increasing flush magnitude rather than increasing flush frequency. Two years after burial, SSD promoted emergence through increased flush magnitude and flush frequency. The promotional effects of SSD on emergence were strongest within 500 d°C following SSD; however, low levels of SSDinduced emergence were detected as late as 3000 d°C following SSD. Accordingly, stale seedbed practices that eliminate weed seedlings should occur within 500 d°C of disturbance, because few seedlings emerge after this time. However, implementation of stale seedbed practices will probably cause slight increases in weed population densities throughout the year. Compared with the common population, local populations exhibited reduced variance in total emergence measured within sites and across SSD treatments, suggesting that C. album adaptation to local pedo-climatic conditions involves increased consistency in SSD-induced emergence.

Reinterpreting ‘generic structure’: evolution, application and limitations of a concept

This paper traces the evolution of thegeneric structure concept in system dynamics and discusses the different practical uses to which they have been put. A review of previous work leads to the identification of three different views of what a ‘generic structure’ is and, hence, what transferability means. These different views are distinguishable in application as well as in theory. Examination of these interpretations shows that the assumptions behind them are quite distinct. From this analysis it is argued that it is no longer useful to treat ‘generic structure’ as a single concept since the unity it implies is only superficial. The conclusion is that the concept needs unbundling so that different assumptions about transferability of structure can be made explicit, and the role of generic structures as generalisable theories of dynamic behaviour in system dynamics theory and practice can be debated and clarified more effectively.