Sensate lateral arm flap for defects of the lower leg.

Ideally, reconstruction of lower extremity soft tissue defects includes not only an esthetically pleasing 3-dimensional shape and solid anchoring to the underlying structures to resist shear forces, but should also address the restoration of sensation. Therefore, we present a prospective study on defect reconstruction of the lower leg and ankle to evaluate the role of sensate free fasciocutaneous lateral arm flap and the impact of sensory nerve reconstruction. Thirty patients were allocated randomly to the study group (n = 15) that obtained end-to-side sensate coaptation using the lower lateral cutaneous brachial nerve to the tibial nerve using the epineural window technique, or to the control group reconstructed without nerve coaptation. At 1-year follow-up the patients were evaluated for pain sensation, thermal sensibility, static and moving 2-point discrimination, and Semmes-Weinstein monofilament tests. Data from both groups were compared and statistically analyzed with the Mann-Whitney U test and the Fisher exact test. Flaps of the study group reached a static and moving 2-point discrimination and Semmes-Weinstein monofilament tests nearly equal to the contralateral leg area and significantly better than flaps of the control group. Donor damage morbidity of the tibial nerve did not occur. To our point of view resensation should be carried out by end-to-side neurorrhaphy to the tibial nerve because of the superior restoration of sensibility.

Evaluation du taux de filtration glomérulaire chez l'enfant : étude clinique et perspectives

Le "Chest wall syndrome" (CWS) est défini comme étant une source bénigne de douleurs thoraciques, localisées sur la paroi thoracique antérieure et provoquées par une affection musculosquelettique. Le CWS représente la cause la plus fréquente de douleurs thoraciques en médecine de premier recours. Le but de cette étude est de développer et valider un score de prédiction clinique pour le CWS. Une revue de la littérature a d'abord été effectuée, d'une part pour savoir si un tel score existait déjà, et d'autre part pour retrouver les variables décrites comme étant prédictives d'un CWS. Le travail d'analyse statistique a été effectué avec les données issues d'une cohorte clinique multicentrique de patients qui avaient consulté en médecine de premier recours en Suisse romande avec une douleur thoracique (59 cabinets, 672 patients). Un diagnostic définitif avait été posé à 12 mois de suivi. Les variables pertinentes ont été sélectionnées par analyses bivariées, et le score de prédiction clinique a été développé par régression logistique multivariée. Une validation externe de ce score a été faite en utilisant les données d'une cohorte allemande (n= 1212). Les analyses bivariées ont permis d'identifier 6 variables caractérisant le CWS : douleur thoracique (ni rétrosternale ni oppressive), douleur en lancées, douleur bien localisée, absence d'antécédent de maladie coronarienne, absence d'inquiétude du médecin et douleur reproductible à la palpation. Cette dernière variable compte pour 2 points dans le score, les autres comptent pour 1 point chacune; le score total s'étend donc de 0 à 7 points. Dans la cohorte de dérivation, l'aire sous la courbe sensibilité/spécificité (courbe ROC) est de 0.80 (95% de l'intervalle de confiance : 0.76-0.83). Avec un seuil diagnostic de > 6 points, le score présente 89% de spécificité et 45% de sensibilité. Parmi tous les patients qui présentaient un CWS (n = 284), 71% (n = 201) avaient une douleur reproductible à la palpation et 45% (n= 127) sont correctement diagnostiqués par le score. Pour une partie (n = 43) de ces patients souffrant de CWS et correctement classifiés, 65 investigations complémentaires (30 électrocardiogrammes, 16 radiographies du thorax, 10 analyses de laboratoire, 8 consultations spécialisées, et une tomodensitométrie thoracique) avaient été réalisées pour parvenir au diagnostic. Parmi les faux positifs (n = 41), on compte trois angors stables (1.8% de tous les positifs). Les résultats de la validation externe sont les suivants : une aire sous la courbe ROC de 0.76 (95% de l'intervalle de confiance : 0.73-0.79) avec une sensibilité de 22% et une spécificité de 93%. Ce score de prédiction clinique pour le CWS constitue un complément utile à son diagnostic, habituellement obtenu par exclusion. En effet, pour les 127 patients présentant un CWS et correctement classifiés par notre score, 65 investigations complémentaires auraient pu être évitées. Par ailleurs, la présence d'une douleur thoracique reproductible à la palpation, bien qu'étant sa plus importante caractéristique, n'est pas pathognomonique du CWS.

Fatores de risco para diabetes mellitus tipo 2 entre adolescentes

O estudo teve como objetivo identificar fatores de risco para diabetes tipo 2 (DM 2) em uma população de adolescentes de escolas particulares de Fortaleza, Brasil. Foram avaliados 794 alunos, de 12 a 17 anos, em doze escolas, nos meses de maio, junho, agosto e setembro de 2007. Aplicou-se um formulário abordando aspectos sociodemográficos, IMC, pressão arterial, glicemia capilar e sedentarismo. Aproximadamente 24% dos participantes tinham o IMC elevado, 65% eram sedentários e 51% tinham antecedentes familiares de DM 2. Naqueles com maior renda, 73,5% tinham antecedentes familiares de DM 2 (p=0,04). Por volta de 39% dos adolescentes apresentavam pelo menos dois fatores de risco para DM 2. A maior parte dos fatores de risco para DM 2, identificados neste estudo, são modificáveis, portanto passíveis de intervenções preventivas no contexto escolar.

Validation of diagnosis criteria for acquired sensory neuronopathy. a francophone multicenter study

Recently published criteria using clinical (ataxia or asymmetrical distribution at onset or full development, and sensory loss not restricted to the lower limbs) and electrophysiological items (less than two abnormal lower limb motor nerves and at least an abolished SAP or three SAP below 30% of lower limit of normal in the upper limbs) were sensitive and specific for the diagnosis of sensory neuronopathy (SNN) (Camdessanche et al., Brain, 2009). However, these criteria need to be validated on a large multicenter population. For this, a database collecting cases from fifteen Reference Centers for Neuromuscular diseases in France and Switzerland is currently developed. So far, data from 120 patients with clinically pure sensory neuropathy have been collected. Cases were classified independently from the evaluated criteria as SNN (53), non-SNN (46) or suspected SNN (21) according to the expert's diagnosis. Using the criteria, SNN was possible in 83% (44/53), 23.9% (11/46) and 71.4% (15/21) of cases, respectively. In the non-SSN group, half of the patients with a diagnosis of possible SSN had an ataxic form of inflammatory demyelinating neuropathy. In the SNN group, half of those not retained as possible SNN had CANOMAD, paraneoplasia, or B12 deficiency. In a second step, after application of the items necessary to reach the level of probable SNN (no biological or electrophysiological abnormalities excluding SNN; presence of onconeural antibody, cisplatin treatment, Sj ¨ ogren's syndrome or spinal cord MRI high signal in the posterior column), a final diagnosis of possible or probable SNN was obtained in, respectively, 90.6% (48/53), 8.8% (4/45), and 71.4% (15/21) of patients in the three groups. Among the 5 patients with a final non-SNN but initial SNN diagnosis, 3 had motor conduction abnormalities (one with CANOMAD) and among the 4 patients with a final SNN but initial non-SSN diagnosis, one had anti-Hu antibody and one was discussed as a possible ataxic CIDP. These preliminary results confirm the sensitivity and specificity of the proposed criteria for the diagnosis of SNN.

Cytotoxic effects of FGF2-saporin on bovine epithelial lens cells in vitro.

PURPOSE: To test the ability of two preparations of FGF2-saporin, either FGF2 chemically conjugated to saporin (FGF2-SAP) or genetically engineered FGF2-saporin (rFGF2-SAP) to inhibit the growth of bovine epithelial lens (BEL) cells in vitro when in solution and when immobilized on heparin surface-modified (HSM) polymethylmethacrylate (PMMA) intraocular lenses (IOLs). METHOD: Bovine epithelial lens cells were incubated with various concentrations FGF2-saporin for as long as 4 days. The number of surviving cells was determined by counting the number of nuclei. Because FGF2 binds to heparin, FGF2-saporin was incubated with HSM PMMA IOLs; excess toxin was washed off, and the BEL cells were grown on the FGF2-saporin-treated IOLs (HSM and non-HSM) for 4 days. Cell density was determined by image analysis. RESULTS: Both FGF2-SAP and rFGF2-SAP were highly cytotoxic (nM range), with rFGF2-SAP 10 times less active than FGF2-SAP. FGF2-saporin bound to the surface of HSM IOLs and eluted by 2M NaCl retained its activity. Toxin bound to HSM IOLs killed more than 90% of the BEL cells placed on the IOL surface within 4 days. The ability of FGF2-saporin to prevent the growth of cells on the IOL surface was strictly dependent on the presence of heparin on the IOL. CONCLUSIONS: FGF2-saporin is bound to HSM PMMA IOLs and prevents the growth of epithelial cells on the surface of the lens.

Conhecimento de mulheres com diabetes mellitus sobre cuidados pré-concepcionais e riscos materno-fetais

Objetivou-se descrever o perfil reprodutivo de mulheres com diabetes mellitus (DM) e verificar o nível de conhecimento destas quanto aos riscos maternos e fetais e os cuidados pré-concepcionais. Estudo exploratório, que contou com a participação de 106 mulheres, realizado no Centro Integrado de Hipertensão e Diabetes, de março a julho de 2009. As variáveis reprodutivas foram: número de gestações, partos e abortos e planejamento da gravidez. Os dados foram coletados por meio de entrevista que seguiu um formulário pré-estabelecido. O perfil reprodutivo de mulheres com DM mostrou-se permeado de riscos e repercussões reprodutivas negativas à saúde materna e fetal. Das 106 (100%) mulheres estudadas, 44 (41,5%) apresentaram conhecimento moderado sobre os cuidados pré-concepcionais e 58 (54,7%) conhecimento limitado sobre os riscos maternos e fetais. Faz-se necessário oferecer informações às mulheres a fim de promover o conhecimento sobre os riscos maternos e fetais e os cuidados pré-concepcionais.

SOSelectiva

Este trabajo nace de la idea de que el sistema de recogida selectiva de residuos en Barcelona puede mejorarse. Para respaldar esta hipótesis previa, hemos utilizado tanto datos de organismos oficiales (La EMMA o el Ayuntamiento de BCN por citar algunos de los más relevantes) como encuestas realizados por nosotros mismos y que han sido distribuidas a una muestra de aproximadamente 180 personas de la ciudadcondal.Con la información obtenida de estas fuentes, podemos afirmar que, definitivamente, estábamos en lo correcto: el sistema puede mejorarse.En este punto es necesario ser precisos y explicar qué entendemos por mejorar.Concretamente, lo que nosotros queremos hacer es incrementar el porcentaje de cantidad recogida de papel-cartón, vidrio y envases ligeros sobre el total consumido deestos materiales. Este será nuestro objetivo durante todo el estudio. Y, como el lectordescubrirá, hemos propuesto dos vías para conseguir tal cosa: incrementar el número decontenedores (método extensivo) o implementar una nueva tecnología (métodointensivo). El planteamiento y desarrollo de estas dos propuestas será la piedra angulardel trabajo.Ahora bien, a modo de anticipo, ¿qué podemos decirle al lector sobre estas vías?¿Consiguen el objetivo que se proponen? ¿Son aplicables? La respuesta es que, como severá a lo largo de estas páginas, el método extensivo consigue efectivamente incrementar el porcentaje de cantidad recogida mientras que la vía intensiva, aunque tiene la ventaja de que es capaz de reducir los costes que se derivan del proceso (costesde transporte…) y es consecuente con políticas de sostenibilidad medioambiental (higieniza los residuos, reduce enormemente los desechos destinados a depósitoscontrolados e incineradoras) presenta la gran desventaja de que precisamente por seruna tecnología nueva, no hay suficientes datos para estudiar su comportamiento en unperiodo de tiempo largo. Por tanto, en este estudio se abogará por la estrategia extensiva, en pos de mostrar un análisis más coherente y de implementación rápida.

Recollida selectiva porta a porta

Sap la gent quin és el model de recollida selectiva porta a porta? Ajuda aquest sistema a la preservació del medi ambient o es tracta simplement d’un mite? Això és el que ens vam preguntar nosaltres a l’inici del treball. Aquest model s’aplica des del mes de novembre del 2008 a Palau-solità i Plegamans, un municipi del Vallès Occidental. A partir de l’estudi d’aquest cas pràctic hem volgut analitzar la viabilitat d’aquest sistema i la seva acceptació social. Comprovarem que aquest sistema és deficitari i que els impostos juguen un paper molt important en el finançament del sistema actual.Un altre objectiu és comparar el sistema porta a porta que hi ha actualment amb el que es va proposar en l’anterior legislatura, basat en la divisió del poble en dues zones amb models de recollida diferents. Concretament, ens interessa saber si va ser una bona idea canviar de sistema i, tal com veurem més endavant, la resposta és afirmativa.També volem saber si aquest model es podria aplicar a altres municipis amb densitat i poblacions diferents. A través d’aquest treball, veurem que la densitat de la població és un dels factors més importants, malgrat que influeixen altres elements (per exemple, l’acceptació social).

Creació d'una discoteca: Amber Club

El món de l’oci nocturn és un món que molta gent disfruta però que ningú acaba de conèixer. Tothom sap per on van els trets principals, però ningú es preocupa profundament per saber realment com funciona, quins són els costos, si els beneficis són elevats, o si és difícil aconseguir que un negoci sigui viable. Aquest, doncs, ha estat el pilar sobre del qual s'ha construït el nostre treball.A través de la creació d’una discoteca i el seu anàlisi cost-benefici hem intentat esbrinar quina és la viabilitat d’un projecte d’aquestes característiques, analitzant tots els costs que s’han d’incórrer, i realitzant un supòsit a la baixa dels ingressos que s’obtindrien.Per dur a terme aquest anàlisi hem realitzat 200 enquestes repartides per les diferents universitats de la província de Barcelona. D’aquesta manera hem pogut analitzar si hi ha demanda per una nova discoteca i quines són les preferències del consumidor.Una vegada fet aquest anàlisi, hem procedit a la recerca d'un local que s'adapti a les nostres necessitats i a les mostrades pel públic enquestat. Al no voler entretenir-nos en qüestions com la decoració del local, o l’adaptació d’aquest a tota la normativa vigent, hem partit del supòsit que un local que actualment funciona estigués disponible pel lloguer, concretament Opium Mar. Hem introduït un seguit de reformes que creiem necessàries pel millor funcionament, i a continuació hem començat amb les estratègies de màrqueting i la determinació dels serveis oferts.Dos punts seran claus per tal que la discoteca funcioni correctament. Primer de tot, el fet que sigui coneguda per un gran públic, i llavors que aquest públic en qüestió estigui content amb el serveis oferts. És per això que hem destinat un gran nombre de recursos en màrqueting (pàgina web, mailing , SMS...) i ens hem centrat també amb tots els serveis que podem oferir (guarda-roba, sales VIP...). La reputació, ens han dit molts responsables, pot ser l’eina que pot fer del teu projecte una gran font d’ingressos, o el contrari, una gran pèrdua de diners.També ens hem centrat en el personal i el subministrament de begudes, calculant així quin és el nombre de treballadors necessaris per tirar endavant el projecte, i quin és l’estoc de begudes òptim que hem de tenir per cobrir tota la demanda.Finalment, hem tingut en compte els impostos que hem d'incórrer a un projecte d'aquest tipus, ja sigui impost de societats, el cost de la llicència per exercir l'activitat, IVA, etc.Una conclusió és clara després de la realització d’aquest projecte: la nostra discoteca és rendible, i per extensió qualsevol altre amb un nombre considerable d’assistència. El valor afegit que crea un projecte d’aquestes magnituds és tan gran, i la despesa mitjana de la gent en oci nocturn tan considerable, que un projecte capaç de portar un nombre de clients relativament petit en funció del local, pot presentar beneficis

[Arménie] / Armineh Johannes, photogr.

Enregistrement : 00-00-1990 - 00-00-1993

Morbidades e qualidade de vida de idosos com diabetes mellitus residentes nas zonas rural e urbana

Este estudo objetivou descrever as variáveis sociodemográficas e comparar as morbidades e a qualidade de vida (QV) dos idosos com diabetes mellitus (DM) residentes nas zonas urbana e rural. A amostra foi composta de 271 idosos da zona urbana e 104 da rural que autorreferiram DM. Utilizou-se análise descritiva e, na comparação das localidades, realizou-se ajuste para a idade por meio de regressão logística e linear múltipla (p < 0,05). Os idosos da zona rural eram mais jovens, casados e possuíam maior escolaridade e renda em relação àqueles da área urbana. Além disso, apresentaram maior escore de QV nos domínios físico e relações sociais e nas facetas autonomia, atividades passadas, presentes e futuras e intimidade, em relação aos do espaço urbano, nos quais se verificou maior número de comorbidades. Os idosos com DM da zona rural apresentaram, de forma geral, melhores condições de saúde em comparação aos que residiam na área urbana.

The Paediatric Rheumatology International Trials Organisation provisional criteria for the evaluation of response to therapy in juvenile dermatomyositis.

OBJECTIVE: To develop a provisional definition for the evaluation of response to therapy in juvenile dermatomyositis (DM) based on the Paediatric Rheumatology International Trials Organisation juvenile DM core set of variables. METHODS: Thirty-seven experienced pediatric rheumatologists from 27 countries achieved consensus on 128 difficult patient profiles as clinically improved or not improved using a stepwise approach (patient's rating, statistical analysis, definition selection). Using the physicians' consensus ratings as the "gold standard measure," chi-square, sensitivity, specificity, false-positive and-negative rates, area under the receiver operating characteristic curve, and kappa agreement for candidate definitions of improvement were calculated. Definitions with kappa values >0.8 were multiplied by the face validity score to select the top definitions. RESULTS: The top definition of improvement was at least 20% improvement from baseline in 3 of 6 core set variables with no more than 1 of the remaining worsening by more than 30%, which cannot be muscle strength. The second-highest scoring definition was at least 20% improvement from baseline in 3 of 6 core set variables with no more than 2 of the remaining worsening by more than 25%, which cannot be muscle strength (definition P1 selected by the International Myositis Assessment and Clinical Studies group). The third is similar to the second with the maximum amount of worsening set to 30%. This indicates convergent validity of the process. CONCLUSION: We propose a provisional data-driven definition of improvement that reflects well the consensus rating of experienced clinicians, which incorporates clinically meaningful change in core set variables in a composite end point for the evaluation of global response to therapy in juvenile DM.

Comparative resilience of clinical perimetric tests to induced levels of intraocular straylight.

PURPOSE: To investigate the effect of intraocular straylight (IOS) induced by white opacity filters (WOF) on threshold measurements for stimuli employed in three perimeters: standard automated perimetry (SAP), pulsar perimetry (PP) and the Moorfields motion displacement test (MDT).¦METHODS: Four healthy young (24-28 years old) observers were tested six times with each perimeter, each time with one of five different WOFs and once without, inducing various levels of IOS (from 10% to 200%). An increase in IOS was measured with a straylight meter. The change in sensitivity from baseline was normalized, allowing comparison of standardized (z) scores (change divided by the SD of normative values) for each instrument.¦RESULTS: SAP and PP thresholds were significantly affected (P < 0.001) by moderate to large increases in IOS (50%-200%). The drop in motion displacement (MD) from baseline with WOF 5, was approximately 5 dB, in both SAP and PP which represents a clinically significant loss; in contrast the change in MD with MDT was on average 1 minute of arc, which is not likely to indicate a clinically significant loss.¦CONCLUSIONS: The Moorfields MDT is more robust to the effects of additional straylight in comparison with SAP or PP.

Characterization of the Morphological and Molecular Changes Associated with Diabetic Peripheral Neuropathy in Type 2 Diabetes

More than 246 million individuals worldwide are affected by diabetes mellitus (DM) and this number is rapidly increasing (http://www.eatlas. idf.org). 90% of all diabetic patients have type 2 DM, which is characterized by insulin resistance and b-cell dysfunction. Even though diabetic peripheral neuropathy (DPN) is the major chronic complication of DM its underlying pathophysiological mechanisms still remain unknown. To get more insight into the DPN associated with type 2 DM, we characterized the rodent model of this form of diabetes, the db/db mice. The progression of pathological changes in db/db mice mimics the ones observed in humans: increase of the body weight, insulin insensitivity, elevated blood glucose level and reduction in nerve conduction velocity (NCV). Decreased NCV, present in many peripheral neuropathies, is usually associated with demyelination of peripheral nerves. However, our detailed analysis of the sciatic nerves of db/db mice exposed for 4 months to hyperglycemia, failed to reveal any signs of demyelination in spite of significantly reduced NCV in these animals. We therefore currently focus our analysis on the structure of Nodes of Ranvier, regions of intense axo-glial interactions, which also play a crucial role in rapid saltatory impulse conduction. In addition we are also evaluating molecular changes in somas of sensory neurons projecting through sciatic nerve, which are localized in the dorsal root ganglia. We hope that the combination of these approaches will shed light on molecular alterations leading to DPN as a consequence of type 2 DM.