Evaluation of Sperm Kinetics and Plasma Membrane Integrity of Frozen Equine Semen in Different Storage Volumes and Freezing Conditions

In the present study, different freezing systems (Styrofoam box and Mini Digitcool ZH 400) and storage volumes (0.5- and 0.25-mL straws) were compared with regard to sperm kinetics and plasma membrane integrity of frozen and thawed semen. For that, three ejaculates from four animals were frozen in Styrofoam box and Mini Digitcool ZH 400 machine. The 0.5-mL straws were thawed at 46°C for 20 seconds, and the 0.25-mL straws were thawed at 46°C for 12 seconds. Statistical analysis was performed using program R of descriptive analysis box plot, followed by analysis of variance using PROC MIXED of SAS 9.1 package. Variances of 5% were considered as different. There was no interaction between the straw sizes and volumes; however, statistical differences were observed between the semen storage volumes. The 0.5-mL straws had higher total motility (%), progressive motility (%), average path velocity (μm/s), straight-line velocity (μm/s), curvilinear velocity (μm/s), and rapid sperm percentage (%) than the 0.25-mL straws. However, plasma membrane integrity analysis did not differ between the two straws. Thus, it is possible to conclude that equine sperm cryopreserved in 0.5-mL straws has better sperm kinetics than when stored in 0.25-mL straws. Additionally, it is possible to conclude that automated systems that enable faster freezing rates result in a seminal quality that is similar to the one obtained by the conventional system using Styrofoam boxes. © 2013 Elsevier Inc.

Digestibility, fermentation and rumen microbiota of crossbred heifers fed diets with different soybean oil availabilities in the rumen

The goal of this study was to evaluate the effects of different soybean oil availabilities on the intake and partial and total digestibility of dry matter (DM) and nutrients, rumen fermentation parameters, efficiency of microbial synthesis, and the rumen microbiota of crossbred beef heifers. Nine crossbred heifers fitted with rumen and duodenal cannulae were evaluated in a triple 3 × 3 Latin square design with three treatments and three periods in three simultaneous repetitions. Heifers approximately 18 months old, with mean initial and final body weights of 316.3±28.8 and 362.6±34.4 kg, respectively, were fed a diet containing 600. g/kg of corn silage and 400. g/kg concentrate with a 58.0. g/kg fat content in the total diet. The sources of lipids included soybean grain, rumen-protected fat, and soybean oil. The statistical analyses were conducted using PROC MIXED from SAS, and the means were compared using Tukey's test (P<0.05). Dietary lipid sources did not affect nutrient intake (P>0.05). Except the apparent digestibility of organic matter (P=0.024), the apparent digestibility of the other nutrients did not differ among the treatment groups. Regarding body nitrogen retention, the soybean grain treatment was more effective than the rumen-protected fat treatment (P=0.045); however, the soybean oil treatment did not differ from the other two treatments. In relation to the efficiency of microbial protein synthesis (g. N/kg of organic matter apparently digested in the rumen corrected for microbial organic matter), the soybean oil and soybean grain treatments were more efficient than the rumen-protected fat treatment (P=0.001). Animals fed rumen-protected fat had larger numbers of protozoa (P<0.001) and fungi (P<0.001) than those supplemented with soybean grain and soybean oil. The dietary lipid sources did not affect pH, the molar concentration of propionate and total volatile fatty acids (P>0.05), whereas the concentrations of ammonia nitrogen and acetate were higher in animals fed with rumen-protected fat than in those submitted to the other treatments. The use of different soybean oil availabilities did not affect nutrient intake; however, treatments with soybean oil and soybean grain were more efficient regarding nutrient intake than rumen-protected fat because they reduced the numbers of fungi and protozoa and consequently improved the efficiency of microbial protein synthesis. © 2013 Elsevier B.V.

Efeito da adição de parede celular de levedura sobre a digestibilidade, microbiota, ácidos graxos de cadeia curta e aminas fecais e parâmetros hematológicos e imunológicos de cães

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Adaptação de bovinos ao confinamento: avaliação do temperamento e dos comportamentos social e alimentar

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Determinação da taxa de ovulação e sua relação com diâmetro folicular e isoformas de mRNA para receptor de LH, em vacas da raça Nelore

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do estresse térmico calórico na expressão de alguns genes relacionados à implantação e desenvolvimento inicial de embriões Nelore (Bos indicus) e Jersey (Bos taurus) produzidos in vitro

Pós-graduação em Ciências Biológicas (Farmacologia) - IBB

Análise de genes associados à deposição de gordura em bovinos da raça Nelore

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ajuste de modelos não lineares e estimativas de parâmetros genéticos para produção de ovos de uma linhagem de poedeiras White Leghorn

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fatores que afetam a viabilidade e a proporção do sexo de embriões bovinos produzidos in vitro em programa de sexagem comercial

Pós-graduação em Ciência Animal - FMVA

Comparação entre diferentes taxas de sêmen na viabilidade espermática em garanhões de alta e baixa congelabilidade

Pós-graduação em Medicina Veterinária - FMVZ

Estudo da diversidade de basidiomicetos poliporóides de fragmentos florestais remanescentes do noroeste paulista

Pós-graduação em Microbiologia - IBILCE

Exigências líquidas de macrominerais para crescimento em cabritos Saanen de diferentes sexos

Pós-graduação em Zootecnia - FCAV

Mês de parição, condição corporal e resposta a protocolos de inseminação artificial em tempo fixo em vacas de corte primíparas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da concentração pré e pós-ovulatória de progesterona em protocolos de IATF em fêmeas nelore

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desempenho reprodutivo de novilhas Nelore pré-púberes expostas à progesterona

Pós-graduação em Zootecnia - FMVZ