In vitro antimicrobial activity of aqueous extracts from Lentinula edodes isolates against Colletotrichum sublineolum and Xanthomonas axonopodis pv. Passiflorae

The aim of this study was to evaluate the antimicrobial activity of aqueous extracts from fruiting bodies of different isolates of Lentinula edodeson the pathogens Colletotrichum sublineolum, the causal agent of anthracnose in sorghum, and Xanthomonas axonopodispv. passiflorae, the causal agent of bacterial spot in passion fruit. Results showed that the aqueous extracts from isolates LE JAB-K and LE 95/01 significantly reduced C. sublineolumspore germination,while the isolate LE 96/22 was the only one to inhibit the pathogen mycelial growth. However, all L. edodesisolates showed inhibitory effect on C. sublineolumappressorium formation. Regarding X. axonopodispv. passiflorae, the aqueous extracts from all L. edodesisolates significantly reduced the in vitromultiplication of the bacterium. However, antimicrobial activity was lost when the extracts were autoclaved, demonstrating their thermolabile property. The aqueous extract from isolate LE 96/22 was also partially purified by anion exchange chromatography and fraction V exhibited high inhibitory activity on the in vitromycelial growth of C. sublineolum, while the multiplication of X. axonopodispv. passifloraewas inhibited by fractions IV, V and VII. Thus, L. edodesisolates were shown to produce compounds exhibiting antifungal and antibacterial activities against phytopathogens, which are mainly concentrated in fraction V.

In vitro effect of substrate, temperature and photoperiod on Phakopsora pachyrhizi urediniospore germination and germ tube growth

In vitro experiments were conducted to assess the effects of substrate, temperature and time of exposure to temperature and photoperiod on P. pachyrhizi uredospore germination and germ tube growth. The following substrates were tested: water-agar and soybean leaf extract-agar at different leaf concentrations (0.5, 1.0, 2.0 and 4.0 g of leaves and 15g agar/L water), temperatures (10, 15, 20, 25, 30, and 35oC) and times of exposure (1, 2, 3, 4, 5, 6, 7, and 8 hours) to temperature and 12 different photoperiods. The highest germination and germ tube length was found for the soybean leaf extract agar. Maximum P. pachyrhizi uredospore germination was obtained at 21.8 and 22.3°C, and maximum germ tube growth at 21.4 and 22.1°C. The maximum uredospore germination was found at 6.4 hours exposure, while the maximum germ tube length was obtained at 7.7 h exposure. Regarding photoperiod, the maximum spore germination and the maximum uredospore germ tube length were found in the dark. Neither spore germination nor uredospore germ tube growth was completely inhibited by the exposure to continuous light.

PASSIONS AND EVIL IN KANT'S PHILOSOPHY

In this paper, I aim at relating passions to evil in Kant's philosophy. I begin by explaining the difference between affects and passions in the textAnthropology from a Pragmatic Point of View. Kant claims that both affects and passions are illnesses of the mind, because both affect and passion hinder the sovereignty of reason. I show that passions are worse than affects for the purpose of pure reason. Second, I relate affects and passions to the degrees of the propensity to evil in theReligion. I analyze the idea of an ethical community as a way to overcome the evil, which goes beyond political and anthropological solutions suggested by Kant.

Computational modeling for irrigated agriculture planning. Part II: risk analysis

Techniques of evaluation of risks coming from inherent uncertainties to the agricultural activity should accompany planning studies. The risk analysis should be carried out by risk simulation using techniques as the Monte Carlo method. This study was carried out to develop a computer program so-called P-RISCO for the application of risky simulations on linear programming models, to apply to a case study, as well to test the results comparatively to the @RISK program. In the risk analysis it was observed that the average of the output variable total net present value, U, was considerably lower than the maximum U value obtained from the linear programming model. It was also verified that the enterprise will be front to expressive risk of shortage of water in the month of April, what doesn't happen for the cropping pattern obtained by the minimization of the irrigation requirement in the months of April in the four years. The scenario analysis indicated that the sale price of the passion fruit crop exercises expressive influence on the financial performance of the enterprise. In the comparative analysis it was verified the equivalence of P-RISCO and @RISK programs in the execution of the risk simulation for the considered scenario.

Jäähyväiset seksille

Kirjallisuusarvostelu

Jesus and the Gentiles

Doktorsavhandlingen Jesus and the Gentiles belyser Jesu attityder gentemot hedningarna. Evangelierna nämner endast två konkreta tillfällen där Jesus hjälper en hedning – en syrisk-fenikisk kvinna (Mark. 7:24–30/Matt. 15:21–28) och en officer i Kafarnaum (Matt. 8:5–13/Luk. 7:1–10). För att förstå Jesu attityder gentemot hedningarna är det viktigt att belysa den sociala, religiösa, ideologiska och etniska bakgrunden av Jesu verksamhet. I ljuset av de arkeologiska utgrävningarna och literära källorna är det klart att i början av första seklet bestod den stora majoriteten av Galileens befolkning av judar. Fastän Jesu verksamhet berörde närmast judar började de tidiga kristna med hednamissionen redan under 40-talet. Hur kan denna unika utveckling förklaras? Inom judendomen var hedningarnas möjliga frälsning oftast anknuten till uppfyllelsen av Israels eskatologiska restauration. De tidiga judekristna trodde att Jesus var Messias och att den eskatologiska tiden hade börjat. Dessa premisser gav de tidiga kristna upphov att tolka GT:s skrifter och Jesu ord och mission från en eskatologisk synvinkel som medförde att frälsingen skulle beröra Israel och även hedningarna. Just i ett eskatologiskt sammanhang är det sannolikt att Jesu liknelser om måltiden i Guds rike (Matt. 8:11-12) och om senapsfrön som växer och blir till ett stort träd (Mark. 4:30–32), refererar till hedningar som skall komma in i Guds rike. De tidiga jude-kristnas hednamission har sina rötter i Jesu förståelse om att den eskatologiska tiden har blivit installerad på något sätt. Även om Jesu verksamhet inte berörde direkt hedningar är det klart att enligt vissa av Jesu autentiska ord var han övertygad om att under den kommande eskatologiska uppfyllelsen skulle en del av hedningarna få plats i Guds rike (Matt. 8:11-12; 11:22-24; 12:41-42).

Influência de fontes de nitrogênio sobre o desenvolvimento das gemas axilares de explantes caulinares de Chrysanthemum morifolium Ramat. cultivado in vitro

Foram testadas diferentes fontes de nitrogênio na indução e enraizamento de brotações axilares de Chrysanthemum morifolium. Manteve-se constante o teor de nitrogênio total (60 mM) em todos os tratamentos realizados, variando-se apenas as fontes nitrogenadas. O tratamento E1 constituiu-se do meio MS (Murashige & Skoog 1962) completo. Os demais tratamentos foram: E2 = amônio; E3 = nitrato; E4 = nitrato+uréia (1,65 mM); E5 = nitrato+uréia (3,33 mM); E6 = uréia; E7 = uréia+glutamina e E8 = glutamina. Em nenhum tratamento foram adicionados fitorreguladores. No tratamento E1 houve um favorecimento de desenvolvimento de plantas de crisântemo in vitro tanto da parte aérea como de raízes. Porém, a utilização apenas de nitrato como única fonte de nitrogênio (tratamento E3) foi suficiente para sustentar o desenvolvimento das plantas, pois, para a maioria dos parâmetros analisados não foi observada diferença significativa em relação às plantas mantidas no meio básico de MS. A presença de uréia no meio de cultura incrementou o desenvolvimento de raízes de crisântemo, principalmente quando utilizada em adição ao nitrato (tratamentos E4 e E5), proporcionando um sistema radicular bastante denso. Quando utilizadas como única fonte de nitrogênio, tanto a glutamina (tratamento E7) como o íon amônio (tratamento E2) não se mostraram eficientes. O resultado do uso das fontes mencionadas foi um baixo desenvolvimento das plantas, como evidenciado pelos baixos valores de crescimento obtidos em todos os parâmetros analisados.

Meiotic behavior in wild and domesticated species of Passiflora

The meiotic behavior of fourteen Passiflora taxa was analyzed. The species were grouped according to the n value (6, 9 and 12) for statistical studies. Some species presented tetravalent associations or univalent chromosomes in diakinesis, bivalent formation prevailing. The qui-square test revealed significant differences in the chiasma frequency among species for n = 9 and n = 6 groups. There was predominance of interstitial chiasmata in almost all studied species. The n = 12 group was the only one whose meiotic behavior was considered similar due to the quantity of chiasmata per cell, tendency of interstitial chiasma localization. Some species presented meiotic irregularities, such as laggard and precocious chromosomes in meiosis I. In telophase II the percentages of meiotic irregularities was low. Irregularities in the spindle orientation were presented in higher percentages in the end of meiosis II, and were also responsible for post-meiotic abnormal products. The irregularities observed during meiosis can have influence on the percentage of sterile pollen grains and success of interspecific crossings in Passiflora species.

Hybridization among wild passionflower species

(Hybridization among wild passionflower species). Passion fruits are appreciated for their ornamental value, since their flowers are showy and display a wide variety of colors. In addition, many hybrids have been produced and used in other countries. The genotypes used in selection of plants with ornamental characteristics are hybrid progenies which are used in various crossing strategies. Thus, the aim of this work was to obtain interspecific hybrids, perform backcrossing and obtain progenies from crossings between hybrids, and to determine the reproductive compatibility between the progenitors involved. The percentage of fertilized flowers, germination, and the number of fruits, seeds and plants obtained through crossing were recorded. A series of 374 crossings involved seven species and two hybrids. Crossings such as Passiflora gibertii N. E. Brown vs. P. kermesina Link & Otto and P. gibertii vs. P. alata Curtis did not produce seeds. The largest percentage of fertilized flowers (86%) was recorded for the crossing P. gardneri Mast.vs. P. cincinnata Mast.; yet, the seeds produced did not show endosperm. Interspecific hybrids were obtained from the crossings P. gardneri vs. P. alata, P. watsoniana Mast.vs. P. alata, P. watsoniana vs. P. gardneri and P. gardneri vs. P. gibertii. Seeds generated from backcrossings involving the hybrids P. sublanceolata (sin. P. palmeri var. sublanceolata (Killip) J. M. MacDougal) vs. P. foetida var. foetida L. (HD13-133 and HD13-141) and F2 reached high germination percentages.

Self-, cross- and interspecific pollinations in Passiflora capsularis and P. rubra

This study aimed to characterize the reproductive system of Passiflora capsularis L. and P. rubra L. In vivo controlled pollinations, in vitro pollen germination and pollen-ovule (P:O) ratio evaluation were conducted. In self-pollination, intraspecific and interspecific pollination, P. capsularis showed means of 62.5, 68.7 and 48.4% of fertilized flowers, while in P. rubra, the averages were 67.2, 62.5 and 46.9%, respectively. For in vitro germination, 52.2% of P. capsularis pollen grains germinated while in P. rubra, the percentage was 64.4. The P:O ratio was 22.4 for P. capsularis, and 27.4 for P. rubra, which included them in the category of obligatory autogamous. Passiflora capsularis and P. rubra can reproduce both by self-pollination and cross-pollination, and crossings between the two species succeeded though the success rate was lower than 50%. The characteristics of the reproductive system of both species allow the use of greater range of options on breeding methods for production of ornamental Passiflora plants.

Situs insulae Atlantidis a mari olim absorptae ex mente Aegyptiorum et Platonis descriptio

Julkaisussa: Athanasii Kircheri e soc. Jesu : mundus subterraneus in XII libros digestus. Vol I

Mappa fluxus et refluxus rationes in Isthmo Americano : in freto Magellanico caetarisque Americae litoribus exhibens

Julkaisussa: Athanasii Kircheri e soc. Jesu : mundus subterraneus in XII libros digestus. Vol I

Sotamuistomerkki Suomessa. Voiton ja tappion modaalista sovittelua

Väitöskirjani tarjoaa laajasti tietoa sotamuistomerkeistä koko Suomen alueelta. Työ purkaa suomalaisten sotamuistomerkkien ilmaisutapoja ja modaalisia keinoja, joilla taideteokset sovittelevat voitetun tai hävityn sodan tarinaa ja kokemuksellisia jännitteitä toisiinsa. Suurin osa analyysin kohteena olevista taideteoksista on pystytetty Suomen itsenäisyyden ajalla vapaudenpatsaiksi tai sankarihautojen, taisteluiden ja vakaumuksensa puolesta kuolleiden muistomerkeiksi. Tutkimus painottuu voiton ideologian vaikutukseen sotakuvaston muotoutumisessa. Avainkysymyksiä ovat, miten sotamuistomerkki viestittää poliittis-ideologisia tavoitteita tai miten muistomerkkien figuurien asennot, eleet ja attribuutit välittävät sotatarinan yhteisöllisiä sisältöjä. Tutkimus tarkentuu teosten modaalisiin piirteisiin ja merkityksenmuodostuksen vuorovaikutteisuuteen. Erikoishuomion kohteena ovat aiheiden sisällölliset ristiriidat ja ilmaisun murtumakohdat. Suurten teosmäärien ja aihetyyppien jaottelussa ja analysoimisessa on hyödynnetty ikonografian, kuvaretoriikan ja eleiden tutkimusta. Suomalaisen aineiston vertailukohtina ovat antiikin sotilasaiheinen taide, keskiaikainen Kristuksen kärsimyskuvasto sekä sotamuistomerkkiperinne Saksassa, Ranskassa, Yhdistyneessä kuningaskunnassa ja Amerikan yhdysvalloissa. Sotien muistokultin merkitysten avaamisessa käytetään diskurssianalyysin välineitä. Tutkimus osoittaa, että sotamuistomerkit rakentavat yhteisön turvallisuudentunnetta ja muokkaavat sotilasimagoa maskuliinisten ideaalien ja implisiittisen vihollis- tai vastakuvan varassa. Kansallisen paatoksen ohessa sotamuistomerkit vahvistavat sotilaiden aseveliaatetta ja luovat kuvaa rikkumattomasta yhteishengestä sekä kotirintamasta. Teokset tulkitsevat valmistumisaikansa usein ristiriitaista tunneilmapiiriä ja tulevaisuuden odotuksia sekä neuvottelevat paikallisesta erityisyydestä ja valtakysymyksistä. Veistosten modaaliset keinot suhteessa toimijarooleihin, kuten autonomisuuden korostus, tunteenomainen toiseen tukeutuminen tai sodan velvoitteisiin suuntautuminen perustuvat yleensä figuurien asentoihin. Sen sijaan figuurien eleet ja attribuutit, tärkeimpinä kypärä, ase, univormu ja lumipuku, tarkentavat suuntautumisen tavoitetta ja ideologista sanomaa. Koska sodassa on kyse vaikeasti käsiteltävistä väkivaltakulttuurin ilmiöistä, muistomerkeillä on hämärretty ja muokattu kuvaa historian tapahtumista. Siten teosten välittämät ideat uhrivalmiudesta ja tunteiden hillinnän velvoitteesta auttavat sotatraumojen ja surun kanavoimisessa sekä purkavat tapahtumiin liittyvää häpeää.

De sacramentis, disputationes decem

Invokaatio: I.N.J.

Artificium Delilae mysticum

Invokaatio: In nomine Jesu!