Separation of the glucose-stimulated cytoplasmic and mitochondrial NAD(P)H responses in pancreatic islet β cells

Two-photon excitation microscopy was used to image and quantify NAD(P)H autofluorescence from intact pancreatic islets under glucose stimulation. At maximal glucose stimulation, the rise in whole-cell NAD(P)H levels was estimated to be ≈30 μM. However, because glucose-stimulated insulin secretion involves both glycolytic and Kreb's cycle metabolism, islets were cultured on extracellular matrix that promotes cell spreading and allows spatial resolution of the NAD(P)H signals from the cytoplasm and mitochondria. The metabolic responses in these two compartments are shown to be differentially stimulated by various nutrient applications. The glucose-stimulated increase of NAD(P)H fluorescence within the cytoplasmic domain is estimated to be ≈7 μM. Likewise, the NAD(P)H increase of the mitochondrial domain is ≈60 μM and is delayed with respect to the change in cytoplasmic NAD(P)H by ≈20 sec. The large mitochondrial change in glucose-stimulated NAD(P)H thus dominates the total signal but may depend on the smaller but more rapid cytoplasmic increase.

C.U.C.P. alumni journal.

25

New crocodilians from the Upper Paleocene of western Colorado / by Karl P. Schmidt --

v.6:no.21(1938)

Crocodile hunting in Central America / by Karl P. Schmidt, Chief Curator, Dept. of Zoology.

no.15(1952)

Turtles of the Chicago area / by Karl P. Schmidt, Curator of Amphibians and Reptiles.

no.14(1938)

The truth about snake stories / by Karl P. Schmidt, Asst. Curator of Reptiles and Amphibians.

no.10(1929)

Notes of proceedings and cases determined in the high court of errors and appeals, the court of chancery, and the orphans court of the state of Delaware, 1792-1832

Contains summaries of cases heard by the Delaware Supreme Court and the Delaware Appeals Court in the counties of Sussex, Kent, and Newcastle covering a variety of legal topics. Supposedly based on Wilson's Red Book.

Report of the Secretary of State on the claim of Patterson and Murguiendo with a further argument of the claimants.

Mode of access: Internet.

Women's Volleyball Team, 2000 UM

Back Row: head coach Mark Rosen, assistant coach Leisa Rosen, assistant coach Jun Liu, Cohey Soji, Sarah Allen, Sarah Behnke, Alija Pittenger, Shannon Melka, Shawna Olson, student assistant coach Sarah Paura, strength trainer Jason Cole, athletic trainer Joel Pickering

Front Row: Lisa Gamalski, Kris Kraut, Chantel Reedus, Katrina Lehman, Dana Chapman, Nicole Poquette, Joanna Fielder, Nicole Kacor, Annie Maxwell, Erin Moore

... Hombres de América (Montalvo-Bolívar-Rubén Darío) Discursos parlamentarios

"Discursos parlamentarios; selección de los discursos pronuncisdos en la honorable Cámara de representantes del Uruguay por José Enrique Rodó" p. [160]-255

A brief history of early Kelly Field, 1916-1918 /

Shipping list no.: 88-213-P.

Monuments arabes et moresques de Cordove, Séville et Grenade : dessinés et mesurés en 1832 et 1833 /

Special t.-p. for Granada: Souvenir de Grénade et de L'Alhambra. Paris, 1837.