970 resultados para Factorial experimental design


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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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The aim of this work was to evaluate the application of different concentrations of ascorbic acid on Orange Flesh melon. Whole Melons were sanifi ed with 500 mg L-1 of sodium hypochlorite for 10 minutes and the cuts into cubes with 100 mg L-1 for 1 minute before being tested under different concentrations of ascorbic acid (0, 1, 2 and 3%) in immersion at room temperature for 10 minutes. After drainage, the cuts were packed in PET packages lined with polyethylene fi lm of 18 µm and stored at 5 ± 1°C and 85 ± 5% of RH for 8 days, being evaluated every 2 days. Physicochemical, microbiological and sensorial analyses were performed. The experimental design utilized for the experiment was the completely randomized in factorial scheme. Ten replicates were used for non-destructive analyses and 3 replicates were used for destructive ones. The application of ascorbic acid reduced the loss of mass; the fruits presented a low population of psychrotrophic bacterias, fi lamentous, fungi and yeasts, reduction of soluble solids, pH and fi rmness and, consequently, extended postharvest life of the fruits by 2 days. The appearance, fl avor and taste were also affected. The application of 1% of ascorbic acid was the best treatment for the fresh cuts “Orange Flesh” melons.

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The objective of the present work was to evaluate the in vitro mycelial growth of ten L. edodes strains (LED 12, LED 20, LED 25, LED 27, LED 33, LED 35, LED 51, LED 55, LED 58 and LED 75) submitted to the temperatures of 15, 20 and 25 ºC. An agar medium prepared with eucalyptus wood extract and soy bran was used and radial measurement of the mycelial growth of L. edodes strains was performed. The experimental design was totally randomized, in a 10 x 3 factorial scheme. Each treatment corresponded to a Petri plate and consisted of 5 repetitions. It was verified that L. edodes growth is influenced by the incubation temperature, that is the temperature of 25 ºC was the most favorable for the mycelial growth of all L. edodes strains, especially for LE 75, LE 55, LE 33 and LE 12 strains, which obtained the highest mycelial growth averages at 25 ºC at the end of the cultivation cycle.

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Lentinus strigosus (Schwein.) Fr. is an exploitable edible mushroom occurring in the Brazilian Amazon, being part of a huge diversity of edible mushrooms which are little grown. The use of regional waste is recommended to reduce production costs of any kind of edible mushroom. Thus, the mycelial growth of L. strigosus in culture media based on regional wood waste extract by using substrates based on Protium puncticulatum, Cariniana micrantha and Caryocar glabum sawdust, supplemented with 20% of wheat bran (Triticum aestivum), corn bran (Zea sp.) or rice bran (Oryza sp.) was observed. Eucalyptus (Eucaliptus sp.) sawdust was used for comparison with the other wood wastes because it is commonly used in the cultivation of edible fungi. The experimental design employed was totally randomized, in 4 x 3 factorial scheme (sawdust x bran), adding up 12 treatments with 5 repetitions, being that each repetition corresponded to a Petri dish, totalizing 60 dishes, incubated at 35 ºC. The diameter of the colony was daily evaluated until the fungus reached the borders of the Petri dish in one of the treatments. After that period, the media based on P. puncticulatum sawdust obtained thebest results of mycelial growth, showing potential to be used as an alternative residuein a future production of L. strigosus in the state of Amazonas.

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The effect of the contaminant fungi Trichoderma sp. and Chaetomium olivacearum on the cultivation of the ABL 99/30 and ABL 04/49 isolates of A. blazei in two compost formulations made up with tyfton (Cynodom dactylon) and oat (Avena sativa) was evaluated. The experimental design was a totally randomized 2 x 2 x 3 factorial design with 6 repetitions. The experimental unit consisted of 12-12.5 kg of wet compost. During the spawning, 150 g of Trichoderma sp. and C. olivacearum were added to the compost. The experiment was carried out in a climatized room, with humidity between 75-90% and temperature of 28º C. The productivity averages of the ABL 99/30 isolate of A. blazei were higher than those of ABL 04/49 and Trichoderma sp. and C. olivacearum negatively influenced the production of A. blazei. The different composts (based on tyfton and oat straw) did not influence the production of basidiomata.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The root growth is genetically controlled. There is variability among cultivars and soil factors such as toxicant Al and low contents of Ca and Mg can harm it. The objective of this study was to evaluate the root growth and the shoot growth of millet cultivars in response to liming. The experiment was carried out in pots with 13 dm3 of soil, in greenhouse conditions until 62 days after the emergence of the plants. The used experimental design was completely randomized, in a 2x4 factorial scheme, that is to say,two painço cultivars (AL Mogi e AL Tibagi), and four level of calcareous (0,0; 0,83, 1,67 e 3,34 g dm-3 ), with four replications. Liming promotes increase of the root system and of the aerial parts of the cultivars. The AL Tibagi cultivar presents a root system more tolerant to the low saturation by bases in comparison to the AL Mogi, however, the maximum root growth was occurred in saturations at around 64%. Millet cultivars in high acidity conditions reduce root diameter at the expense of the growth in length.

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Two compost formulations, based on Braquiaria straw (Brachiaria sp.), a conventional one and a spent one, were tested in the cultivation of ABL 99/30 and ABL 04/49 strains of Agaricus blazei. The experimental design was in a completely randomized factorial scheme with four treatments (two strains of A. blazei x two types of compost) and 30 repetitions. Each experimental unit consisted of a box with 10 to 10.5 kg of moist fresh compost. According to the results obtained, the loss of organic matter of the composts was affected by the A. blazei strain and the type of compost used. The traditional compost lost a higher organic matter content compared to the spent compost, and the ABL 99/30 strain caused a higher loss of organic matter in the composts compared to the ABL 04/49 strain. Yield, biological efficiency, mass and number of basidiomata produced were similar between the conventional and the spent compost, as well as the chemical analysis of the produced basidiomata. However, the A. blazei strains showed some differences among each other, the basidiomata of strain ABL 04/49 obtained a higher percentage of crude protein in their composition, compared to the ABL 99/30, in both composts. Thus, the utilization of spent compost in the cultivation of A. blazei did not impair the basidiomata yield nor their nutritional value, demonstrating it to be a good option to be used as an ingredient in the compost formulation for the A. blazei cultivation.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The objective of the work was to evaluate the in vitro mycelial growth of five A. blazei strains (ABL-05/53, ABL-04/49, ABL-03/44, ABL-99/30 and ABL-02/51) when submitted to the temperatures of 20 and 25 ºC. In a laminar flow chamber, discs of the strains were inoculated in the middle of Petri’s plates containing CA (compost-agar) medium and incubated in BOD. After 48 hours, measurements of the mycelial growth began, with the help of a ruler with scale in millimeters, by means of four equidistant measurements, until the moment when the fungal colony reached near the edges of the Petri’s plate in one of the treatments. The experimental design was totally randomized, in 5 x 2 factorial design. Each treatment consisted of seven repetitions, corresponding to one Petri’s plate, totalizing seventy experimental units. We verified that A. blazei growth is influenced by incubation temperature, being that the temperature of 25 ºC was more favorable for the mycelial growth of all A. blazei strains tested, with attention for ABL-04/49 and ABL-03/44 strains, which obtained the highest averages for mycelial growth under this temperature condition at the end of the cultivation cycle.

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We evaluated the effect of gamma irradiation doses (0, 125, 250, and 500 Gy) in control of psychrotrophic bacteria in different strains of Agaricus bisporus (ABI-07/06, ABI-05/03, and PB-1) during storage, cultivated in composts based on oat straw (Avena sativa) and Brachiaria spp. The experimental design was completely randomized in a factorial scheme 4  2  3 (irradiation doses  composts  strains), with 24 treatments, each consisting of 2 replicates, totaling 48 experimental units (samples of mushrooms). The mushrooms collected from all culture conditions were packaged in plastic polypropylene with 200 g each and subjected to Cobalt-60 irradiator, type Gammacell 220, and dose rate 0.740 kGy h–1 , according to the treatments. Subsequently, the control (nonirradiated) and other treatments were maintained at 4 ± 1°C and 90% relative humidity (RH) in a climatic chamber to perform the microbiological analysis of mushrooms on the 1st and 14th day of storage. According to the results, it was found that the highest mean colony psychotrophic count, after 14 days of storage, was observed in strain ABI-07/06 1.30 × 108 g -1 most probable number (MPN) in nonirradiated mushrooms, coming from Brachiaria grass-based compost, and this same strain under the same storage conditions, coming from the same type of compost that underwent a dose of 500 Gy, obtained a significant reduction in mean colonies of psychrotrophic bacteria (2.25 × 104 g –1 MPN). Thus, the irradiation doses tested favored reducing the number of colonies of psychrotrophic bacteria, regardless of the type of compound and strain of A. bisporus.