Phylogenetic relationships among two species of golden monkey and three species of leaf monkey inferred from rDNA variation

Restriction maps of rDNA repeats of five species of Colobinae and three outgroup taxa, Hylobates leucogenys, Macaca mulatta, and Macaca irus, were constructed using 15 restriction endonucleases and cloned 18S and 28S rRNA gene probes. The site variation between Rhinopithecus roxellana and Rhinopithecus bieti is comparable to that between Presbytis francoisi and Presbytis phayrei, implying that R. bieti is a valid species rather than a subspecies of R. roxellana. Phylogenetic analysis on the 47 informative sites supports the case for Rhinopithecus being an independent genus and closely related to Presbytis. Furthermore, branch lengths of the tree seem to support the hypothesis that the leaf monkeys share some ancestral traits as well as some automorphic characters.

Avian comparative genomics: reciprocal chromosome painting between domestic chicken (Gallus gallus) and the stone curlew (Burhinus oedicnemus, Charadriiformes) - An atypical species with low diploid number

The chicken is the most extensively studied species in birds and thus constitutes an ideal reference for comparative genomics in birds. Comparative cytogenetic studies indicate that the chicken has retained many chromosome characters of the ancestral avia

No accelerated evolution of 3 ' UTR region in human for brain-expressed genes

The difference in cognitive skills between humans and nonhuman primates is one of the major characters that define our own species. It was previously hypothesized that this divergence might be attributable to genetic differences at gene expression level,

Morphometry, length-weight relationship and biology of Sepia aculeata (d'Orbigny, 1848) from Mumbai coast, India

Seventeen morphometric characters of Sepia aculeata of Mumbai coast have been studied and the relationships of morphometric characters with dorsal mantle length (DML) were established. The characters compared showed a fair to high degree of correlation ('r' 0.63-0.99). Number of arm suckers and shell rings were related with DML. The shell rings also showed high degree of correlation with DML ('r' 0.79-0.95). However, the relationship between arm suckers and DML was not so good ('r' 0.1-0.4). The length-weight relationship is described as W=0.1821336 L sub(2.801102). Food and feeding analysis confirm the carnivorous feeding behaviour of the species. Mature females found in all months indicate that it has prolonged spawning season with two peaks, september and march-april. Absolute fecundity ranged from 214 to 4143 eggs.

Allozyme and morphological variation in four hatchery stocks of Thai pangas, Pangasius hypophthalmus in Mymensingh, Bangladesh

Genetic and morphological characters of four hatchery population (Shambhuganj, Brahmaputra, Anudan and Bhai-Bhai) of Thai pangas, Pangasius hypophthalmus in Mymensingh region of Bangladesh was studied using morphological characters and allozyme markers from 29 November 2001 to 29 November 2002. A total of 14 morphometric and 6 meristic characters were verified, among which 3 morphometric (BDA, PELFL and HW) and 2 meristic characters (AFR, CFR) of Anudan hatchery population were found to be significantly higher (p>0.001) than those of the other three hatchery populations. Brahmaputra hatchery population was also significantly higher in two meristic characters (PCFR and CFR). For allozyme electrophoresis nine enzyme markers were used viz.: Esr-1*, G3pdh-2*, Gpi-1*, Gpi-2*, Ldh-1*, Ldh-2*, Mdh-1*, Mdh-2* and Pgm* where three loci (Esr-1*, Gpi-2* and Pgm*) were polymorphic (p>0.95) in Anudan and Brahmaputra hatchery populations. The mean proportion of polymorphic loci per population was higher (33.3%) in Brahmaputra and Anudan hatchery populations. Also the expected heterozygosity levels were 0.149 and 0.177 in Brahmaputra and Anudan hatchery populations, respectively. Based on Nei's (1972) genetic distances, the UPGMA dendrogram grouped the populations into two clusters. The Brahmaputra and Anudan populations are in one group; Shambhuganj, and Bhai-Bhai populations are in the second group. High genetic variation in Thai pangas was observed in the Brahmaputra and Anudan hatchery populations and less variation in the other two hatchery populations.

Experimental pathogenesis of Aeromonas hydrophila bacteria in shing Heteropneustes fossilis(Bloch)

Pathogenicity of Aeromonas hydrophila bacteria was tested on the stinging catfish Heteropneustes fossilis. Before artificial infection the morphological, biochemical and physiological characters of Aeromonas hydrophila were studied. The infections were done by two different methods, viz., intramuscular (IM) and intraperitoneal (IP) injection. In infection experiment, each group of 10 fish were injected either intramuscularly or intra peritoneally with one dose higher than the LD50 dose (9.6 x 107 CFU/fish). All the fish tested died within 1 to 9 days. Both in cases of intramuscular and intraperitoneal injection, external pathology were found. Haemorrhagic lesions were evident at the site of injection. The posterior end of the body surface was found to develop greyish-white lesion that was extended up to caudal fin. Hyperemic anal region and the fin bases were also observed. Total bacterial loads in liver, kidney and intestine were determined. Aeromonas hydrophila could be isolated from liver, kidney and intestine of the experimentally infected fish. In case of intramuscular injection the highest and the lowest bacterial load was found to be 2.4 x 107 CFU/g of liver and 2.1 x 102 CFU/g of kidney and in case of intraperitoneal injection they were found to be 3.6 x 106 CFU/g of kidney and 1.2 x 104 CFU/g of kidney respectively. It was concluded that A. hydrophila could cause serious disease condition to Heteropneustes fossilis and its pathogenesis in the fish was also very efficient.

Growth performance and morphological variations of local and Thai climbing perch (Anabas testudineus, Bloch)

Climbing perch locally known as koi (Anabas testudineus) is a popular food fish in our country. Thai climbing perch was introduced in Bangladesh from Thailand. To explore the variation in growth performance and orphological features of local and Thai climbing perch a study was undertaken. The highest gain in length, weight and SGR were found in Thai koi 12.23±0.38 cm, 55.83±0.53 g and 7.92±0.11 %/day respectively. Fourteen morphometric characters were studied where eleven (TL, SL, HL, HBD, LBD, DFL, PECFL, PELFL, AFL, UJL and LJL) showed significant difference (p<0.01) in Thai koi from the local ones. Of the meristic characters no. of dorsal fin rays (hard), anal fin rays (hard), caudal fin rays and scale along lateral line (upper and lower) as recorded from the Thai koi were significantly higher (p<0.01) than that of the local koi. The no. of dorsal fin rays (soft) in Thai koi were also significantly higher (p<0.05) from that of local koi. The number of vertebra were also variable in local and Thai climbing perch (25 in case of local koi and 26 in case of Thai koi). Hence, the results obtained form the present study satisfy the characteristics of A. testudineus which reveals that both the local and Thai koi belongs to the same species. Growth performance of Thai koi was better compared to local koi reared in same conditions.

Seasonal variations of bacterial flora of fresh oil sardines (Sardinella longiceps)

An elaborate study was made on the qualitative and quantitative seasonal variations in the bacterial flora of fresh oil sardines and their biochemical reactions. It was observed that the total bacterial loads and their phosphorescent and biochemical characters were influenced by changes in seasons. During monsoon season total bacterial count was high. Mesophiles predominated during summer, but phosphorescent bacteria were less. Winter favoured the selection of biochemically less active groups of bacteria.

A phylogeny of Chinese leaf monkeys using mitochondrial ND3-ND4 gene sequences

The phylogeny of Chinese leaf monkeys, especially the snub-nosed monkeys (Rhinopithecus), has not been thoroughly investigated using molecular sequence data, perhaps due to their rarity in the wild and their poor representation in institutional collections. Despite several proposed classifications, systematic relationships of these species remain poorly defined and this has hindered their conservation. To clarify the phylogenetic relationships of the leaf monkey clade in China, we sequenced the mitochondrial ND3, ND4L, ND4, tRNA(Arg), tRNA(His), tRNA(Ser), and tRNA(Leu) genes for Rhinopithecus bieti, R. roxellana, Trachypithecus francoisi, T. f. leucocephalus, and T. phayrei as well as Pygathrix nemaeus and Colobus guereza. We included a rotal of 2252 characters for each individual, excluding gaps in primary sequences. Our interpretation of the results from character- and distance-based phylogenetic analyses suggest that (1) Pygathrix nemaeus is sister to Rhinopithecus rather than to Trachypithecus though it is quite divergent from the former; (2) the Yunnan snub-nosed monkey, Rhinopithecus bieti, represents a valid species; (3) the white-headed leaf monkey is not a distinct species, but instead is a subspecies of Trachypithecus francoisi (T. f. leucocephalus), though it should still be considered a separate evolutionarily significant unit (ESU); and (4) because two individuals of the Phayrei's leaf monkey, T. phayrei, are genetically distinct from one another, a more extensive revision of the taxonomy of this putative species in China is needed. These results, plus ongoing work on the molecular systematics of the entire Asian leaf monkey radiation, can provide a sound basis for identifying the appropriate units of conservation for this endangered group of primates.

A new species of Paramesotriton (Caudata : Salamandridae) from Guizhou Province, China

We describe a new species of salamander, Paramesotriton zhijinensis, from Guizhou Province, China. The generic allocation of the new species is based on morphological and molecular characters. In morphology, it is most similar to Paramesotriton chinensis

Photosynthetic bacteria in the marine environment at Porto-Novo

Sediment and water samples were collected from mangrove and estuarine biotopes at fortnightly intervals. The physico-chemical characters of the overlying water were studied. In the mangrove biotope maximum temperature (31.5°C) and in the estuarine biotope maximum salinity (35.6‰) were recorded during the summer season, whereas in post-monsoon period the sulphate content was increased to 516 p.p.m. and the pH was reduced to 7.4. Invariably both in the enriched sediment and water samples four major peaks (at wavelengths 460, 705, 772 and 850 nm) and two minor peaks (at wavelengths 580 and 663 nm) of absorption spectra were noticed. A pure culture of Chromatium sp., isolated from mangroves sediment, showed three peaks of absorption spectra at wavelengths, 500, 580 and 850 nm. The effect of sodium chloride on the growth of Chromatium sp., was also studied and it was observed that maximum growth occurred in the range 1-3% sodium chloride concentration. This isolate was also capable of utilizing various sulphur and carbon compounds. Glycerol and glucose did not show any specific effect whereas pyruvate, malate and acetate increased the growth.

A re-description of the little known rabbit fish Siganus lineatus (Cuvier and Valenciennes) (Pisces:Siganide) with notes on siganid fishes of the Vembanad Lake

Siganus lineatus (Cuv. and Val) is reported from the coast of peninsular India for the first time and a detailed description of it is given based on morphology and meristic characters. It differs from the other allied species of the genus Siganus Forskal in having an oval body without spots and having fifteen parallel longitudinal golden yellow bands, relatively larger last dorsal spine, maxillary position far below the level of the orbit and cheek with distinct rows of scales. S. javus (Linnaeus) and S. canaliculatus (Park) were also obtained from the Vembanad Lake. Short notes on the habitat, occurrence, abundance, seasonal and geographic distribution in relation to fluctuating hydrological conditions of the Vembanad Lake are incorporated together with a key for their identification.

Molecular genetic basis for the rhino mouse from Chinese KM subcolony

Both the rhino mouse and hairless mouse resulted from hairless gene mutation, but they show different phenotypes of skin physiology. The rhino mouse has more similar histological characters to human papular alopecia. Therefore rhino mouse is a good experimental animal model for human papular alopecia. This study reports a hairless mouse named rhino KIZ, arose from KM colony in Kunming Institue of Zoology, by systematic studies on morphology, skin histopathology, gene sequence, pedigree and protein domain analysis. The results demonstrate that a C-to-T transition in exon 11 of hr gene (The mutant gene has been applied for a Chinese patent (patent No. 03135280)) results in the rhino KIZ. The rhino KIZ with clear genetic mechanism will be a useful animal model.

Identification of the postlarval Penaeus (Crustacea, Decapoda, Penaeidae) appearing along shore waters

Diagnostic characters for the identification of postlarval Penaeus appearing along the coastal waters of the Philippines are reviewed and categorized, based on materials from the wild and laboratory. Presently there are 7 adult known species belonging to the genus Penaeus around Panay Island and its adjacent waters, namely: P. monodon, P. semisulcatus, P. merguiensis, P. indicus, P. japonicus , P. canaliculatus, and P. latisulcatus.

Study morphologic, phylogenic relationships DNA barcoding of of identified decapod group (Crustacean) of Persian Gulf and Oman Sea.

Phylogenetic relationships among all described species (total of 12 taxa) of the decapoda, were examined with nucleotide sequence data from portions of mitochondrial gene and cytochrome oxidase subunit I (COI). The previous works on phylogeny proved that the mitochondrial COI gene in crustacean is a good discriminative marker at both inter- and intra-specific levels. We provide COI barcode sequences of commertial decapoda of Oman Sea, Persian Gulf, Iran. Industrial activities, ecologic considerations, and goals of the decapoda Barcode of Life campaign make it crucial that species of the south costal be identified. The reconstruction of evolut phylogeny of these species are crucial for revealing stock identity that can be used for the management of fisheries industries in Iran. Mitochondrial DNA sequences were used to reconstruct the phylogeny of the Penaeus species of marine shrimp. For this purpose, DNA was extracted using phenol- chloroform well as CTAB method. The evolutionary relationships among 12 species of the decapoda were examined using 610 bp of mitochondrial (mt) DNA from the cytochrome oxidase subunit I gene. Finally the cladograms were compared and the resulted phylogenetic trees confirmed that the Iran's species origin is Indo-west pacific species. Iran's species, which were not grouped with the other decapoda taxa seem to always form a sister clade with Indo-west pacific species with strong bootstrap support 100%. The result completely agrees with the previously defined species using morphological characters.However, we still lack any comprehensive and clear understanding of phylogenetic relationships in this group.