Report on a special investigation of the City of Pacific Junction for the period July 1, 2008 through June 30, 2013

Report on a special investigation of the City of Pacific Junction for the period July 1, 2008 through June 30, 2013

Surface-functionalized ultrasmall superparamagnetic nanoparticles as magnetic delivery vectors for camptothecin.

Drug-nanoparticle conjugates: The anticancer drug camptothecin (CPT) was covalently linked at the surface of ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) via a linker, allowing drug release by cellular esterases. Nanoparticles were hierarchically built to achieve magnetically-enhanced drug delivery to human cancer cells and antiproliferative activity.The linking of therapeutic drugs to ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) allowing intracellular release of the active drug via cell-specific mechanisms would achieve tumor-selective magnetically-enhanced drug delivery. To validate this concept, we covalently attached the anticancer drug camptothecin (CPT) to biocompatible USPIOs (iron oxide core, 9-10 nm; hydrodynamic diameter, 52 nm) coated with polyvinylalcohol/polyvinylamine (PVA/aminoPVA). A bifunctional, end-differentiated dicarboxylic acid linker allowed the attachment of CPT to the aminoPVA as a biologically labile ester substrate for cellular esterases at one end, and as an amide at the other end. These CPT-USPIO conjugates exhibited antiproliferative activity in vitro against human melanoma cells. The intracellular localization of CPT-USPIOs was confirmed by transmission electron microscopy (iron oxide core), suggesting localization in lipid vesicles, and by fluorescence microscopy (CPT). An external static magnetic field applied during exposure increased melanoma cell uptake of the CPT-USPIOs.

Agreed-upon procedures report on the City of Central City, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Central City, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Blue Grass, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Blue Grass, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Bedford, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Bedford, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Pleasant Plain, Iowa for the period January 1, 2014 through December 31, 2014

Agreed-upon procedures report on the City of Pleasant Plain, Iowa for the period January 1, 2014 through December 31, 2014

Agreed-upon procedures report on the City of Patterson, Iowa for the period December 1, 2013 through November 30, 2014

Agreed-upon procedures report on the City of Patterson, Iowa for the period December 1, 2013 through November 30, 2014

Agreed upon procedures report on the City of Galt, Iowa for the period August 1, 2013 through July 31, 2014

Agreed upon procedures report on the City of Galt, Iowa for the period August 1, 2013 through July 31, 2014

Report on a special investigation of the Center for Behavioral Health for the period January 1, 2011 through May 21, 2013

Report on a special investigation of the Center for Behavioral Health for the period January 1, 2011 through May 21, 2013

Agreed-upon procedures report on the City of Cambridge, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Cambridge, Iowa for the period August 1, 2013 through July 31, 2014

The effect of a period of intensive exercise on the isoform test to detect growth hormone doping in sports.

OBJECTIVE: The major objective of this study was to investigate the effects of several days of intense exercise on growth hormone (hGH) testing using the World Anti-Doping Agencies hGH isoform differential immunoassays. Additionally the effects of circadian variation and exercise type on the isoform ratios were also investigated. STUDY DESIGN: 15 male athletes performed a simulated nine day cycling stage race. Blood samples were collected twice daily over a period of 15days (stage race+three days before and after). hGH isoforms were analysed by the official WADA immunoassays (CMZ Assay GmbH). RESULTS: All measured isoform ratios were far below the WADA decision limits for an adverse analytical finding. Changes in the isoform ratios could not be clearly connected to circadian variation, exercise duration or intensity. CONCLUSIONS: The present study demonstrates that the hGH isoform ratios are not significantly affected by exercise or circadian variation. We demonstrated that heavy, long term exercise does not interfere with the decision limits for an adverse analytical finding.

Report on a special investigation of the band program of the Monticello Community School District for the period January 1, 2009 through December 31, 2013

Report on a special investigation of the band program of the Monticello Community School District for the period January 1, 2009 through December 31, 2013

Agreed-upon procedures report on the City of Gilman, Iowa for the period September 1, 2013 through August 31, 2014

Agreed-upon procedures report on the City of Gilman, Iowa for the period September 1, 2013 through August 31, 2014

Agreed-upon procedures report on the City of Mount Ayr, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Mount Ayr, Iowa for the period July 1, 2013 through June 30, 2014

Electrical conductivity testing of corn seeds as influenced by temperature and period of storage

The objective of this work was to evaluate the effects of temperature (10, 20, 30, 20/10 and 30/10ºC) and period of storage on electrical conductivity (EC) in four seed lots of corn (Zea mays L.), as well as the mineral composition of the soaking solution. EC test determines indirectly the integrity of seed membrane systems, and is used for the assessment of seed vigor, because this test detects the seed deterioration process since its early phase. The research comprised determinations of water content, germination, accelerated aging (AA), cold (CT) and EC vigor tests, and determinations of Ca2+, Mg2+ and K+ release to the solution, after seed soaking of four corn seed lots. The evaluations were performed each four months during a period of 16 months. For statistical analysis, a completely randomized split plot design was used with eight replications. Except for seed lots stored at 10ºC, all vigor evaluations revealed a decline in vigor, but AA and CT showed more sensitiveness to declines of seed physiological quality than EC. Potassium was the main leached ion regardless of the storage temperature.