Intergeneric catla - rohu hybrids: F2 generation

Intergeneric catla-rohu hybrids were bred through hypophysation and about 5.25 lakh spawn of F2 generations were produced. The rate of survival from fertilized eggs to spawn ranged from 62.5 to 96.4% at 26-30 degree C.

A simple technique of controlled breeding of carps in semi-arid environment

A semi-arid environment is a major constraint for production of carp seed through hypophysation. At a water temperature above 31 degree C fishes often fail to respond to induced breeding or produce partial or full eggs with fairly less fertilization, leading to their mortality during embryonic development. Field trials with Labee rohita and Cyprinus carpio communis prove that hypophysation followed by stripping and hatching in a water medium with reduced temperature (below 31 degree C) through controlled use of ice-water and water showers can result in 50-60% fertilization of eggs and 50-72% hatching for L. rohita, and 40-90% fertilization and 49-77% hatching for C. carpio communis. Simultaneous breeding experiments of the species in normal water temperature (>31 degree C) showed negative results.

Recent advances in our knowledge of the biology and conservation of mahseers

Mahseers (Tor khudree), at one time considered to be of single species, are now represented by 6 valid species distributed all over India. Studies on their food habits, ecology of spawning grounds, eggs, larval development and especially methods of artificial propagation have advanced in recent years. Transport of eggs of Tor khudree by air in moist cotton has been possible for easy distribution. Breeding of T. khudree not only by hypophysation, but even without it in small ponds by manipulation of flow of water, exercise and feed has also been possible. Fry and fingerlings of T. khudree are being distributed to many of the states in the country to help conserve the species.

Fecundity of the snow-trout, Schizothorax plagiostomus (Heckel) from Garhwal Himalaya

Absolute fecundity of Schizothorax plagiostomus varied from 3474 in a fish measuring 345 mm (T.L.) and weighing 315 g to 13916 in a fish measuring 540 mm (T.L.) and weighing 1370 g. The weight of the fish ovaries accounted for 7.02 to 17.78% of the total weight of the fish. The average number of eggs per kg weight of fish and per gram of ovary weight was found to be 10820 and 81 respectively. The fecundity per kg of body weight was found to be 11539. The relationships between fecundity and other parameters (fish length/fish weight/ovary weight/ovary volume/ovary length) and fish length-ovary weight and fish weight-ovary weight were also determined.

Early and better maturity in carp brood stock with control of environment and feeding

Available of carp breeders in their prim state of maturity is a major constraint in hypophysation. Experiments conducted in a fish farm at Naihati, West Bengal, for two consecutive years, 1983-84 and 1984-85, clearly prove that by manipulation of environmental parameters such as metabolites, dissolved oxygen, running water conditions, as also of stock densities and quality and quantity of feed. Catla catla, Hypophythalmichthys molitrix, Labeo rohita, Cirrhina mrigala and Ctenopharyngodon idella can be made to attain better maturity and spawning stage much earlier than normal i.e. even in summer months and the entire stock spawned during the period from March to September. Percentage of successful breeding, quantities of eggs released and fertilised in relation to the body weight of all the species, were also found to be more in comparison to the brood stock raised through the conventional methods.

Occurrence of Chelonibia testudinaria (Linnaeus) (Crustacea: Cirripedia) in coastal waters of Pakistan

Five specimens of sessile barnacle, Chelonibia testudinaria (Linnaeus) were collected, in February 1993, from the back of a green turtle, Chelonea mydas, which was going back to the sea after laying eggs on Sandspit beach (24 degree 49'N; 66 degree 56'E). Chelonibia testudinaria is widely distributed throughout tropical and temperate seas and has only been reported to be found attached to turtles (Newman and Ross, 1976). In spite of its wide distribution it has not been reported from Northern Arabian Sea bordering Pakistan. The only species of genus Chelonibia known to occur in coastal waters of Pakistan is C. patula (Ranzani) recently reported by Javed and Mustaquim (1992). A brief description of C. testudinaria based on the present material is given below. All the specimens are housed in the Centre of Excellence in Marine Biology.

Observations on the mating behavior, spawn mass and larval development of Hydatina physis Linne, 1758 (Cephalaspidea: Hydatinidae) from Karachi

Reproduction of Hydatina physis was studied in a population from Karachi, Pakistan, including mating and egg laying behavior, spawn characteristics and development.Individuals first appear in the field in October and remain until March. The spawning occurs from mid-November till mid-February with a peak in December. During this period the individuals were also observed pairing. In captivity, mating lasts for 30 minutes, second mating occurs two days later. Oviposition occurs in a very interesting and unusual manner. The mother turns "up-side-down" with its food fully expanded and the shell completely hidden underneath, the expanded foot serves as protective cover to the eggs. Eggs are deposited in a complexly folded mass with a short stem and an adhesive disc. Capsules, arranged in a single layer, contain 4-6 eggs each of wich is 70 um in diameter. Development is planktotrophic and veligers hatch after 14 days at a temperature of 26-28 degrees Celsius.

Observations on spawning and egg capsule of Melongena bucephala (Lamarck)(Mollusca: Gastropoda) from the Karachi coast

No work has been done on the reproduction of Melongena bucephala from northern Arabian Sea (Karachi coast). This paper presents a description of spawning, egg capsule and macromophology of eggs of M. bucephala collected from the rocky shore of Buleji on May 28, 1986 at a tidal height of 0.5m. The individual was found in the act of spawning. The egg mass and development of M. bucephala from Karachi coast are also compared with the Hemifusus ternatanus from the Pacific Ocean (Amio, 1963).

Studies on the biochemical composition of some freshwater fishes. Pt. 3. Ovary

Biochemical composition of the spent ovaries of 23 different species of freshwater fishes showed a much lower value for protein and fat with higher moisture content than those of the ripe eggs, suggesting an inverse relationship between fat and moisture. Ovaries during the recovering phase showed fairly high values of phosphorus, but lower than those for the ripe eggs. Higher values of calcium and iron in spent ovaries than those in ripe eggs probably suggest that there is a greater requirement of calcium and iron during the spent phase of the gonads.

Studies on the preparation of fish protein concentrate

The paper deals with the method of preparation of an edible fish protein concentrate from cheap miscellaneous fish. The method consists in cooking the fish with 0.5% glacial acetic acid, and extracting batch—wise, using ethyl alcohol followed by an azeotropic mixture of hexane and alcohol (B. Pt. 58-68°C). The product is finally vacuum dried during which the residual solvent is also removed. The concentrate prepared by this method contains 85% protein of which 96% is pepsin digestible. The product is practically odorless and almost white in color.

Studies on technological problems associated with the processing of cooked frozen prawns. Pt. 1. Preprocess storage conditions of raw material in relation to quality

The chemical and organoleptic properties of prawn held in ice for different days prior to cooking and the changes after freezing and subsequent storage were studied with three different species of prawn viz. Metapenaeus monoceros, Metapenaeus dobsoni and Parapeneopsis stylifera. The optimum period for which the prawn can be kept under ideal conditions of icing prior to cooking has been worked out.

A few observations on the biology of Tachysurus thalassinus Ruppell from Veraval (Gujarat)

The length-weight relationship of T. thalassinus differed in the sexes and, therefore separate equations were obtained; for males log W = -5.1728 4- 3.0495 log L; and for females log W = -5.7456 + 3.2798 log L. The spawning period of this species appears to be restricted to a short period from October to November. Fecundity has been found to range from 33 to 55 mature eggs in specimens of the size range 421-564mm. The sex ratio of males to females was 1.8:1. T. thalassinus is a bottom carnivore, crustaceans ranking first in food followed by fishes, mollusc and polychaetes.

Induction of mitotic and meiotic gynogenesis and production of genetic clones in rohu, Labeo rohita Ham.

Studies were undertaken to produce genetic clones derived from all homozygous mitotic gynogenetic individuals in rohu, Labeo rohita Ham. ln view of this, attempts were made to interfere with the normal functioning of the spindle apparatus during the first mitotic cell division of developing eggs using heat shocks, there by leading to the induction of mitotic gynogenetic diploids in the F1 generation. Afterwards, viable mitotic gynogenetic alevins were reared and a selected mature female fish was used to obtain ovulated eggs which were fertilized later with UV-irradiated milt. Milt was diluted with Cortland’s solution and the sperm concentration was maintained at 10⁸/ml. The UV-irradiation was carried out for 2 minutes at the intensity of 200 to 250 µW/cm² at 28± 1°C. The optimal heat shock of 40°C for 2 minutes applied at 25 to 30 minutes a.f. was used to induce mitotic gynogenesis in first (F1) generation and at 3 to 5 minutes a.f. to induce meiotic gynogenesis in the second (F2) generation. The results obtained are presented and the light they shed on the timing of the mitotic and meiotic cell division in this species is discussed.