Obtenção de anticorpos monoclonais murinos contra antígenos de Staphylococcus aureus resistentes à meticilina por imunização subtrativa

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Análise da frequencia do polimorfismo rs12979860 C/T no gene IL28B em pacientes acometidos por câncer gástrico

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Mutagenic potential of lettuce grown from irradiated seeds

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Phytotoxicity of Tagetes erecta L. and Tagetes patula L. on plant germination and growth

This study evaluated the phytotoxic potential and antioxidant activity of Tagetes patula and Tagetes erecta, and total phenols and flavonoids of the extracts were quantified. Laboratory bioassays for both pre- and post-emergence were performed in Lactuca sativa L. seeds and in the Allium cepa seeds test. The antioxidant activity was evaluated by DPPH radical scavenging and reducing power of iron, besides total phenols and flavonoids quantification in the extracts. Thus, was observed a reduction in the mitotic index when in compared with the negative control. Was observed also a reduction the germination and development of tested seedlings and was verified a considerable antioxidant potential and presence of flavonoids and phenolic compounds in the extracts. According to these results, it is possible to conclude that T. erecta and T. patula have phytotoxic compounds that may enhance and expand their use in the management of organic agriculture, mainly in vegetables.

Produção de ácidos orgânicos por cepas leveduras assimiladoras de xilose

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Seleção, avaliação e utilização de uma levedura personalizada para a produção de etanol

Pós-graduação em Microbiologia - IBILCE

Expressão diferencial de mRNA em células de cultivo infectadas ple herpesvírus bovino 5

Pós-graduação em Medicina Veterinária - FMVZ

Chitosan nanoparticles loaded the herbicide paraquat: The influence of the aquatic humic substances on the colloidal stability and toxicity

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Genotoxic potential of the latex from cotton-leaf physicnut (Jatropha gossypiifolia L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Genotoxicity evaluation of environmental pollutants using analysis of nucleolar alterations

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Qualidade de cebola minimamente processada em diferentes tipos de cortes

The high perishability of the onion has limited its conservation period and caused great post-harvest losses. The need of consuming fresh products has led the market of minimally processed products to considerably rise. Cut operations during the minimal processing may not only cause important biochemical reactions in the onion tissues, but also affect its sensorial quality, resulting in a drastic reduction in the post-harvest life of the product. In this study the influence of different cut types was assessed on the quality of minimally processed onions. 'Baia Periforme'onions were transported to the laboratory, where they were selected, cleaned, classified, peeled, minimally processed into slices, grated and chopped, and subsequently sanitized for 10 minutes in sodium hypochlorite at 100 mg L-1. The product was kept on polystyrene trays covered with plastic film (PVC) as 80 g portions and conserved in a cold chamber at 5 +/- 1 degrees C and 85 +/- 5% RH, during 6 days. Physical, chemical and physico-chemical assays were carried out. Statistical design was completely randomized with 3 treatments and 3 replications (plot group) and 5 replicationss for the control group. For sensorial analysis (aspect and smell), 10 replications were used. To compare means, Tukey test at 5% significance was adopted. During the refrigerated conservation, sliced onions kept more stable levels of soluble solids, titratable acidity, total sugars, reducing and non-reducing sugars. The grated onion was superior considering the sensorial characteristics, indicating that the cut types interfered in these features and the grated onion was more accepted by evaluators.

Effect of a spray formulation on the reproductive parameters of a susceptible population of Rhipicephalus (Boophilus) microplus

The number of studies emphasizing the possible damage that acaricidal spray formulations can cause on engorged female ticks'reproductive parameters is small. The present study evaluated the deleterious effects of a spray formulation (dichlorvos 60% + chlorpyrifos 20%) on the reproductive parameters of a susceptible population of Rhipicephalus (B.) microplus females, using the Stall Test. The ticks were allocated randomly to treatments according to the mean numbers of females detached from each cow on days -3, -2 and -1 and the cattle pen location. The numbers of engorged female ticks that naturally detached from the cattle were counted daily from day 1 to day 30. For each group, 20 detached engorged female ticks or the available number collected daily were evaluated regarding reproductive parameters. Associations of organophosphates demonstrated elevated acaricidal efficacy, as well as deleterious effects on the reproductive parameters of R. (B.) microplus females. The engorged female weight (days 1 to 7), weight of egg masses (days 5 to 10) and larval hatching percentage (days 5 to 19) were decreased (P <= 0.05). It is possible that a formulation can lead to deleterious effects on R. (B.) microplus females when the tick population analyzed shows elevated sensitivity towards a particular formulation. However, further studies need to be conducted.

Efeito de soluções fluoretadas contendo xilitol e sorbitol no número de estreptococos do grupo mutans na saliva de seres humanos

The objective of this study was to assess the effect of 0.05% sodium fluoride solutions containing 2.5% or 12.5% xylitol on the number of Streptococcus mutans in the human mouth. Fifty boys between 8 and 16 years of age participated in this double-blind crossover study. Of the original 50 boys, 33 finished the study. Participants were randomly divided into four groups. The following solutions were employed: placebo solution; 0.05% sodium fluoride solution; 0.05% sodium fluoride + 2.5% xylitol + 2% sorbitol; 0.05% sodium fluoride + 12.5% xylitol + 2% sorbitol. Each solution was used for a 28-day period (20 mL/day, twice a day), with a 10-day washout period between solutions. There were no significant differences (P = 0.32) between the two xylitol-containing solutions (2.5% vs. 12.5%) concerning the number of Streptococcus mutans. However, there was a significant difference between these two xylitol-containing solutions and the sodium fluoride and placebo solutions (P < 0.001). Our results suggest that the 0.05% sodium fluoride solutions containing either 2.5% or 12.5% xylitol caused a significant reduction in the number of Streptococcus mutans.

A flow cytometry method for testing the susceptibility of Cryptococcus spp. to amphotericin B

Human fungal infections have increased at an alarming rate in recent years, particularly in immunocompromised individuals. Cryptococcosis is the second most prevalent systemic fungal infection worldwide, and the most prevalent systemic infection in immunocompromised individuals, representing more than 70% of cases. The incidence of cryptococcosis is high in people with HIV/acquired immunodeficiency syndrome (AIDS), with recent estimates indicating that there are one million cases of cryptococcal meningitis globally per year in AIDS patients. The aim of this research was to develop a rapid flow cytometric antifungal susceptibility test and to compare the results with the standard methods. A reference strain and clinical isolates of Cryptococcus neoformans and Cryptococcus gattii were tested for susceptibility to amphotericin B by flow cytometry using propidium iodide as indicator of viability. Flow cytometry (FC) results were compared with the minimum inhibitory concentration (MIC) values determined by microdilution. The antifungal activity of amphotericin B ranged from MICs of 0.06 to 2μg/ml for the 11 isolates studied. The same results were found by FC. The FC method allows same-day results, assisting in the selection of appropriate antifungal therapies. These results demonstrate an excellent correlation between FC and the classic methods of testing for susceptibility to antifungal agents. This rapid diagnosis method makes it possible to quickly administer effective therapeutic interventions, often saving lives.

Ansiedade e pensamentos automáticos em atletas de basquetebol

Segundo a Confederação Brasileira de Basquetebol (CBB), o basquete é um esporte popular, praticado em todo o mundo por mais de 300 milhões de pessoas. Manoel Tubino (2001, p. 57), aponta é um fenômeno social que atingiu níveis muito complexos de desenvolvimento nas diversas sociedades. Sobre o jovem atleta Cratty (1984) destaca que este acaba vivenciando situações em que seu rendimento, tanto sendo um sucesso como um fracasso se torne visível perante a sociedade, deixando que os sentimentos emocionais e psíquicos se aflorem junto com os fisiológicos influenciando no desempenho. Entre os vários fatores intra e extra quadra que podem influenciar no resultado, estão os aspectos psicológicos e os pensamentos automáticos. Para Cecarelli (2011) um dos fatores que mais afetam os atletas é a ansiedade e está diretamente ligado, a importância da competição e de cada jogo, distância de casa, viagem, mudança de hábitos, adaptação ao clima e fuso horário, entre outros aspectos que provocam possível processamento errôneo do pensamento automático e um aumento da ansiedade. Desta maneira torna-se imprescindível destacar que os aspectos fisiológicos e psicológicos de jovens atletas não se encontram eficientemente maduros para lidar com cobranças e pressões advindas do meio esportivo, cabendo aos sujeitos envolvidos na prática do basquetebol (técnicos, diretores, pais, patrocinadores, psicólogo do esporte e atletas) fazer uso da psicologia do esporte a fim de objetivar-se um bom rendimento. O objetivo deste estudo foi compreender como se dá a relação entre os pensamentos automáticos e a ansiedade. A metodologia deste estudo pautou-se em uma revisão de literatura utilizando os principais referenciais teóricos que circunscrevem os temas principais deste trabalho. Segundo Beck (2005) é tão possível perceber um pensamento, focar nele e avaliá-lo, como é possível identificar e refletir sobre uma sensação...